Abstract:
:Hyperpolarization-activated cyclic nucleotide-gated ion channels (HCN) are key determinants of CNS functions. Here we describe an increase in hyperpolarization-activated current (I(h)) at the beginning of whole-cell recordings in rat layer 5 cortical neurons. For a closer investigation of this I(h) increase, we overexpressed the predominant layer 5 rat subunit HCN1 in HEK293 cells. We characterized the resulting I(h) in the cell-attached and whole-cell configurations. Breaking into whole-cell configuration led to about a 30% enhancement of rat HCN1-mediated I(h) accompanied by a depolarizing shift in voltage dependence and an accelerated time course of activation. This current enhancement is not species specific; for human HCN1, the current similarly increases in amount and kinetics. Although the changes were bound to cytosolic solution exchange, they were independent of cAMP, ATP, GTP, and the phosphate group donor phosphocreatine. Together, these data provide a characterization of heterologous expression of rat HCN1 and suggest that cytosolic contents suppress I(h). Such a mechanism might constitute a reserve in h-channel function in vivo.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Battefeld A,Bierwirth C,Li YC,Barthel L,Velmans T,Strauss Udoi
10.1002/jnr.22475subject
Has Abstractpub_date
2010-11-01 00:00:00pages
3067-78issue
14eissn
0360-4012issn
1097-4547journal_volume
88pub_type
杂志文章abstract::Heme oxygenase-1 (HO1) catalyzes oxidation of the heme molecule in concert with NADPH-cytochrome P450 reductase following the specific cleavage of heme into carbon monoxide, iron, and biliverdin, which is rapidly metabolized to bilirubin. HO1 is a stress-inducible protein that protects cells against oxidative injury, ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10514
更新日期:2003-02-15 00:00:00
abstract::Nicotine has been reported to be neuroprotective in experimental and epidemiological studies. In addition to nicotine, tobacco and cigarette smoke contain cembranoids, which are antagonists of neuronal nicotinic receptors (nAChR). Exposure of hippocampal slices to N-methyl-D-aspartate (NMDA) decreases the population s...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20666
更新日期:2005-12-01 00:00:00
abstract::The combination of nerve growth factor (NGF) and phorbol 12-myristate 13-acetate (PMA) rapidly induced the locomotion of PC12 cells by sequentially stimulating lamellar spreading, ruffling with pinocytosis, and polarization by retraction from the substratum. During migration, cells acquired long processes as a result ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960515)44:4<308::AID-JNR
更新日期:1996-05-15 00:00:00
abstract::Here we show that rabbit Müller cell differentiation from radial glial progenitor cells is accompanied by a decreasing capability to respond to specific stimuli (depolarization and extracellular adenosine 5'-triphosphate [ATP]) with an elevation of intracellular calcium. Intracellular free calcium was recorded in reti...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10406
更新日期:2002-10-15 00:00:00
abstract::To explore the feasibility of cell type-specific gene expression in oligodendrocytes as a possible therapeutic approach for demyelinating diseases, the cell specificity, tissue specificity, and duration of gene expression were investigated using recombinant adeno-associated viral vectors (rAAV) carrying a green fluore...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990215)55:4<504::AID-JNR
更新日期:1999-02-15 00:00:00
abstract::In our in vitro model, rasagiline a selective irreversible monoamine oxidase-B (MAO-B) inhibitor, protected nerve growth factor (NGF)-differentiated PC12 cells from cell death under oxygen and glucose deprivation (OGD). The severity of the OGD insult, as expressed by cell death, was time-dependent. Exposure of the cel...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::In this study, we examined the neuroprotective effect of standardized Bacopa monniera extract (BME: BESEB CDRI-08) against the D-galactose (D-gal)-induced brain aging in rats. Experimental groups were subjected to contextual-associative learning task. We found that the administration of BME in the D-gal-treated group ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23080
更新日期:2012-10-01 00:00:00
abstract::Studies of the effects of hypoglycemia on the brain using neurocognitive testing have suggested that mainly complex functions subserved by secondary and tertiary cortex are affected by mild to moderate hypoglycemia and that intensively treated patients with Type I diabetes mellitus (T1DM) may have altered sensitivity ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21146
更新日期:2007-02-15 00:00:00
abstract::Using a canine model of classical late-infantile neuronal ceroid lipofuscinosis (CLN2 disease), a study was conducted to evaluate the potential pharmacological activity of recombinant human tripeptidyl peptidase-1 (rhTPP1) enzyme replacement therapy administered directly to the cerebrospinal fluid (CSF). CLN2 disease ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23423
更新日期:2014-11-01 00:00:00
abstract::Elk is a member of the eph family of receptor-like tyrosine kinases. Although its function is unknown, elk is postulated to play a role in nervous system development. Using Northern analysis, we examined the developmental regulation of RNAs encoding elk, and several ligands for the eph family of RTKs, the LERKs. Expre...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490420207
更新日期:1995-10-01 00:00:00
abstract::Accumulating evidence points to roles for oxidative stress, amyloid beta (Aβ), and mitochondrial dysfunction in the pathogenesis of Alzheimer's disease (AD). In neurons, the base excision repair pathway is the predominant DNA repair (BER) pathway for repairing oxidized base lesions. Apurinic/apyrimidinic endonuclease ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23565
更新日期:2015-06-01 00:00:00
abstract::Peroxides are generated continuously in cells that consume oxygen. Among the different peroxides, hydrogen peroxide is the molecule that is formed in highest quantities. In addition, organic hydroperoxides are synthesized as products of cellular metabolism. Generation and disposal of peroxides is a very important proc...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.20280
更新日期:2005-01-01 00:00:00
abstract::In the embryonic and postnatal rat retina a cell surface antigen that is detected by monoclonal antibody JONES is distributed in a dorsoventral gradient. Biochemical analysis has determined that the antigen is a modified ganglioside, 9-O-acetyl GD3. In the present study, the distributions of 9-O-acetyl GD3 and its pos...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490210231
更新日期:1988-10-01 00:00:00
abstract::The most visible and, until very recently, the only hypothesis regarding the involvement of microglial cells in Alzheimer's disease (AD) pathogenesis is centered around the notion that activated microglia are neurotoxin-producing immune effector cells actively involved in causing the neurodegeneration that is the caus...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.20093
更新日期:2004-07-01 00:00:00
abstract::The thyroid hormones triiodothyronine (T3) and L-thyroxine appear to enhance regeneration in the peripheral and central nervous system (CNS). The following experiments examine possible metabolic substrates for the action of T3 on the adult rat CNS after spinal hemisection. The protein incorporation of (3H)lysine after...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490060507
更新日期:1981-01-01 00:00:00
abstract::The neonatal lesion with 6-hydroxydopamine (6-OHDA) in rodents induces juvenile hyperactivity and paradoxical hypolocomotor response to psychostimulants, in striking contrast to what is observed when similar lesions are carried out in adults. The early disruption of central dopaminergic pathways is followed by increas...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20245
更新日期:2004-10-15 00:00:00
abstract::To investigate the mechanism underlying the regulation of claudin-5, a tight junction protein that participates primarily in the constitution of the blood-brain barrier by bradykinin (BK), we established a primary culture of rat brain microvascular endothelial cells (BMECs). BMECs were treated with 10(-5) M BK, and ch...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23350
更新日期:2014-05-01 00:00:00
abstract::Most previous studies on spinal cord injury (SCI) have used rodent models. Direct extrapolation of the results obtained in rodents to clinical cases is difficult, however, because of neurofunctional and anatomic differences between rodents and primates. In the present study, the development of histopathologic changes ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20435
更新日期:2005-04-15 00:00:00
abstract::Primary brain tumors, particularly glioblastomas (GB), remain a challenge for oncology. An element of the malignant brain tumors' aggressive behavior is the fact that GB are among the most densely vascularized tumors. To determine some of the molecular regulations occuring at the brain tumor endothelium level during t...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10865
更新日期:2004-01-15 00:00:00
abstract::Gangliosides, sialic acid-containing glycosphingolipids, are ubiquitously expressed in all eukaryotic cells and are localized primarily in the plasma membrane. For a rat pheochromocytoma cell line, PC12, which has been used frequently as a model for investigating events leading to neuronal differentiation, it is gener...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21031
更新日期:2006-11-01 00:00:00
abstract::Neural precursor cells (NPCs) critically regulate brain morphogenesis and recent studies have revealed an unexpectedly high frequency of NPC chromosomal abnormalities and apoptosis in the developing brain. We have shown previously that the apoptotic response of NPCs to genotoxic agents is dependent on p53 and caspase-...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10738
更新日期:2003-11-01 00:00:00
abstract::Previously, we showed that in rat cortical neurons, chronic donepezil treatment (10 microM, 4 days) up-regulates nicotinic receptors (nAChR) and makes neurons more sensitive to the neuroprotective effect of donepezil. Here we examined the mechanism of donepezil-induced neuroprotection in neurons chronically treated wi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21798
更新日期:2008-12-01 00:00:00
abstract::We previously demonstrated that a synthetic retinoic acid receptor agonist, Am80, attenuated intracerebral hemorrhage (ICH)-induced neuropathological changes and neurological dysfunction. Because inflammatory events are among the prominent features of ICH pathology that are affected by Am80, this study investigated th...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23379
更新日期:2014-08-01 00:00:00
abstract::In a number of pathological states of the brain, the activation of the inducible nitric oxide synthase (iNOS) plays a major role. Interleukin (IL)-1beta is believed to be an essential factor in the induction of iNOS. However, little is known about the cascade of events culminating in iNOS expression in vivo. To identi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::SH-SY5Y cells differentiate into neuronal-like cells and express marker proteins like growth-associated protein (GAP-43) and neuropeptide tyrosine when treated with a low concentration (16 nM) of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in the presence of growth factors or serum. Both control and d...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490410609
更新日期:1995-08-15 00:00:00
abstract::Neural stem cells can be propagated in culture as neurospheres, yielding neurons and glial cells upon differentiation. Although the neurosphere model is widely used, the functional properties of the neurosphere-derived neurons have been only partially characterized, and it is unclear whether repeated passaging alters ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20835
更新日期:2006-06-01 00:00:00
abstract::Iron deficiency and chronic mild carbon monoxide (CO) exposure are nutritional and environmental problems that can be experienced simultaneously. We examined the effects of chronic mild CO exposure and iron availability on auditory development in the rat. We propose that chronic mild CO exposure creates an oxidative s...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20495
更新日期:2005-06-01 00:00:00
abstract::Cathepsin D-like activity in homogenates of five brain areas of 3-month-old and 24-month-old Fischer 344 rats was measured. With hemoglobin as substrate at pH 3.2, more than 90% of the activity was inhibited by pepstatin. In each area studied, activity was more than twice as high in the old rat brain: 140-160% higher ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490230412
更新日期:1989-08-01 00:00:00
abstract::Cerebrospinal fluid was collected from the lateral ventricles and cisternal space from beagle dogs under light pentobarbital anesthesia. Homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA), and 3-methoxy-4-hydroxyphenylglyco-sulfate (MOPEG-SO4) levels in the liquor taken from these two regions were evaluated,...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490010513
更新日期:1975-01-01 00:00:00
abstract::Low density dissociated cultures of embryonic rat hippocampal cells were used to study the effects of neurotrophin-3 (NT-3) on neuronal morphogenesis. The results obtained indicate that NT-3 enhances neurite outgrowth and branching; this is a dose-dependent effect, detected in approximately 50% of the neurons, and pre...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490390212
更新日期:1994-10-01 00:00:00