Abstract:
:In early ARDS (Adult Respiratory Distress Syndrome) and other inflammatory pulmonary disorders the lung might benefit from a high local deposition of an active drug, in order to optimize the local concentration without systemic side effects. In this methodological study we used pigs under controlled ventilation. The study was carried out in two steps. In the first part Evans blue dye in NaCl was delivered in aerosolized form. In the second part a dry powder containing FITC (fluorescein-isothiocyanate)-labelled-liposomes in NaCl was delivered in the same way. We evaluated whether there was an even, central, peripheral and/or alveolar deposition, whether the procedure was reproducible, and whether there was an interaction with alveolar macrophages. Sixteen animals under chlormethiazole anaesthesia and intermittent positive pressure ventilation (IPPV) were included in the study. Four animals were sacrificed after nebulization and baseline measurements. Five animals served as controls and received saline i.v. Six animals received endotoxin i.v. (18 micrograms.kg-1.h-1). One animal underwent broncho-alveolar lavage 15 min and 2 h after liposome administration. At the end of each experiment the lungs were inflated with air, excised and dried in a microwave oven. The left lung of each animal was sliced in a reproducible manner and lung-pieces from different regions were analyzed. The Evans blue dye or the phospholipid fraction of the lungs (containing liposomes), was extracted and analyzed spectrofluorometrically. This study shows that it is possible, under reproducible conditions, to administer aerosolized Evans blue dye and liposomes and to achieve a deposition in the terminal airways and/or alveolar spaces. The broncho-alveolar lavage demonstrated an interaction of liposomes with alveolar macrophages. The results imply that liposomes carrying active drugs and administered by inhalation may be used for local pulmonary treatment in early ARDS and other related inflammatory pulmonary diseases.
journal_name
Ups J Med Scijournal_title
Upsala journal of medical sciencesauthors
Forsgren P,Modig J,Gerdin B,Axelsson B,Dahlbäck Mdoi
10.3109/03009739009178581subject
Has Abstractpub_date
1990-01-01 00:00:00pages
117-36issue
2eissn
0300-9734issn
2000-1967journal_volume
95pub_type
杂志文章abstract::Introduction: Carotid endarterectomy (CEA) is a surgical procedure used in the prevention of ischemic stroke. However, this procedure can cause complications of ischemia-reperfusion injury to the brain. Clusterin (CLU) is a cytoprotective chaperone protein that is released from neurons in response to various neurologi...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.1080/03009734.2019.1646359
更新日期:2019-08-01 00:00:00
abstract::For the corticotropin-releasing-hormone stimulation test the number of samplings and measurements are reduced to two--and the ratio between concentrations at 60 min and 0 min is calculated. The difference between the information given by absolute concentrations and ratio is negligible, but by using ratio, the influenc...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009739309179325
更新日期:1993-01-01 00:00:00
abstract::In this report, we present a 68-year-old man with rice body formation in the flexor tendon sheath of the fingers without any inflammatory diseases such as tuberculosis or rheumatoid arthritis. The patient visited our institute in March 2004 with a one-month history of swelling and pain of the right distal forearm. Lab...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.1080/03009730902931408
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Two standard sets of criteria are used to evaluate the tumor response of hepatocellular carcinoma (HCC): RECIST (Response Evaluation Criteria in Solid Tumors) and modified RECIST (mRECIST). The purpose was to compare two tumor response evaluation criteria, RECIST version 1.1 and mRECIST, for HCC treated usin...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章,多中心研究
doi:10.3109/03009734.2012.729104
更新日期:2013-03-01 00:00:00
abstract::This paper discusses briefly: analytical quality and its determinants; design of quality assurance procedures on the basis of quality; specifications and quality-cost models; test quality and medical costs; external quality assessment and harmonization of clinical chemical test results; the potential role of computers...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009738609178519
更新日期:1986-01-01 00:00:00
abstract::During the last decade, capillary electrophoresis (CE) has emerged as an interesting alternative to traditional analysis of serum, plasma and urine proteins by agarose gel electrophoresis. Initially there was a considerable difference in resolution between the two methods but the quality of CE has improved significant...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/2000-1967-219
更新日期:2008-01-01 00:00:00
abstract::Determinations were made of the 2-hour 22Na space and the 1 and 2 hour 51Cr EDTA spaces in a number of tissues. In regions where the two 51Cr-EDTA spaces were similar and smaller than or similar to the 22Na space the former spaces were regarded as measures of the extra-cellular volume. Using previously published data ...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009737909179148
更新日期:1979-01-01 00:00:00
abstract::Antimicrobial susceptibility testing with phenotypic methods requires breakpoints, i.e. a minimum inhibitory concentration (MIC) categorizing micro-organisms into susceptible, intermediately susceptible, and resistant for the relevant antimicrobial agent. Determinations of breakpoints require tools such as the underst...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章,评审
doi:10.3109/03009734.2014.901446
更新日期:2014-05-01 00:00:00
abstract::Alkaline phosphatase from calf intestinal mucosa dephosphorylated histone H1 and fibrinogen that had been phosphorylated with protein kinase C. The reaction velocity was dependent on the ionic strength of the buffer; decreasing with increasing concentration. The pH optimum was around 7, which is lower than pH-optima d...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009739109179262
更新日期:1991-01-01 00:00:00
abstract::For staging of Hodgkin's disease 45 patients underwent lymphography and abdominal computed tomography (CT); in 31 of them ultrasound examination was also performed. In cases of retroperitoneal lymph node enlargement lymphography and CT are diagnostically equivalent, whereas the poorer resolution of ultrasound reduces ...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009738409179500
更新日期:1984-01-01 00:00:00
abstract:BACKGROUND:To achieve a better understanding of the repertoire of receptor tyrosine kinases (RTKs) in adult retinal ganglion cells (RGCs) we performed polymerase chain reaction (PCR), using degenerate primers directed towards conserved sequences in the tyrosine kinase domain, on cDNA from isolated single RGCs univocall...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009731003597119
更新日期:2010-02-01 00:00:00
abstract::The bisphosphonates are potent inhibitors of osteoclastic bone resorption that mainly have been used for treatment of hypercalcaemia secondary to malignancy. We have performed a controlled dose-finding study of oral pamidronate that was given to 60 patients with a history of fracture of the distal forearm, an enhanced...
journal_title:Upsala journal of medical sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.3109/03009739109179272
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:Shoulder external rotation braces used for patients with a first-time shoulder dislocation are designed with a variety of arm positions in abduction as well as external rotation. However, few studies have focused on their immobilizing performance, comfort, or user-friendliness. Especially, there have been no...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009734.2012.744374
更新日期:2013-05-01 00:00:00
abstract::Background: Hepatic de novo lipogenesis (DNL) is ideally measured in very low-density lipoprotein (VLDL)-triacylglycerol (TAG). In the fasting state, the majority of plasma TAG typically represents VLDL-TAG; however, the merits of measuring DNL in total plasma TAG have not been assessed. This study aimed to assess the...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.1080/03009734.2020.1739789
更新日期:2020-08-01 00:00:00
abstract::Previous experiments have demonstrated that neuronal cells within pancreatic islets survive the isolation procedure and constitute an integral part of transplanted pancreatic islets. The aim of the present study was to investigate to what extent immunosuppressive drugs affects the acute survival of intra-islet neurons...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/2000-1967-167
更新日期:2001-01-01 00:00:00
abstract::Epidemiological data on drug-treated diabetes mellitus was obtained by analyzing prescriptions of insulin and oral antidiabetics in the county of Jämtland Sweden. During a 12-year period, from 1971 to 1982, the prevalence of diabetes increased from 1.5% to 2.2%, while there was no significant change in the annual inci...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009738609178494
更新日期:1986-01-01 00:00:00
abstract::We herein outline the rationale for a Swedish cohort consortium, aiming to facilitate greater use of Swedish cohorts for world-class research. Coordination of all Swedish prospective population-based cohorts in a common infrastructure would enable more precise research findings and facilitate research on rare exposure...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.1080/03009734.2018.1556754
更新日期:2019-01-01 00:00:00
abstract::The present investigation on 20 patients after total hip replacement surgery has confirmed that the posttraumatic increase of the fibrinolysis inhibition activity (FIA) in serum and plasminogen-depleted serum is due to the primary fibrinolysis inhibitor (PFI, alpha 2-antiplasmin). This protein exists in at least two f...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009738209178409
更新日期:1982-01-01 00:00:00
abstract:BACKGROUND:There is evidence suggesting that genetic variants in the chondroitin sulfate proteoglycan2 (CSPG2, also known as versican) gene are involved in the pathogenesis of intracranial aneurysms (IAs). Some authors have demonstrated that single nucleotide polymorphisms (SNPs) rs173686 and rs251124 in the promoter r...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/2000-1967-201
更新日期:2007-01-01 00:00:00
abstract::In 2 monkeys of the species Macaca fascicularis the three dorsal column nuclei and the lateral cervical nucleus have been investigated immunocytochemically with antiserum against Substance P. The Substance P labeling was widely spread but rather sparse. It occurred in small structures of the size of boutons in the gra...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009738909178557
更新日期:1989-01-01 00:00:00
abstract::Isokinetic torque of the knee and elbow during maximal extension and flexion and isometric strength of the handgrip, as well as balance and some circulatory variables, were tested in 37 patients one year after an acute stroke. Correlations with locomotion, household work and other activities of daily living (ADL) were...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009738209178405
更新日期:1982-01-01 00:00:00
abstract::Despite the fact that the composition of urine varies a lot during the day, this has essentially been neglected as a factor of importance in determinations of urine bicarbonate. The investigations reviewed in combination with an own study shows that qualitative and quantitative factors in urine composition impacts the...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009738209178420
更新日期:1982-01-01 00:00:00
abstract::The acute phase reaction is an unspecific response to inflammatory stimuli characterized by alterations in the concentration of several plasma proteins. It is of great clinical value to monitor the inflammatory state in patients with rheumatoid arthritis. The erythrocyte sedimentation rate (ESR) and C-reactive protein...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009739809178943
更新日期:1998-01-01 00:00:00
abstract::The vertebral end-plates and the epiphyses from the lumbar region of the spine in juvenile pigs were studied. Cell density of the hyaline cartilage of the vertebral bodies was measured as well as cartilage thickness. The relative bone content in the region of the nucleus pulposus was calculated. A certain negative int...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009738509178481
更新日期:1985-01-01 00:00:00
abstract:INTRODUCTION:Many women and men lack knowledge about fertility, including timing of the fertile window, age-related decline, and lifestyle factors that may impair fertility. The Internet has become an important source of information, but evidence-based information on fertility and reproduction in Swedish on the Interne...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.1080/03009734.2018.1476423
更新日期:2018-06-01 00:00:00
abstract::In this study we describe an unusual complication in a patient suffering from chronic calcifying pancreatitis. The patient had a fistula between the common bile duct, the pancreatic duct, and the portal vein. He received supportive medical treatment and achieved long-term survival. A review of the literature including...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章,评审
doi:10.3109/2000-1967-059
更新日期:2006-01-01 00:00:00
abstract::Oxyhemoglobin, methemoglobin and hematin were found to catalyze xanthine-oxidase-induced peroxidation of phospholipid liposomes, while oxy- and metmyoglobin were inactive in this respect. The peroxidation was inhibited by desferrioxamine and by EDTA. Peroxidation catalyzed by 0.4 microM oxyhemoglobin was decreased by ...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009738809178547
更新日期:1988-01-01 00:00:00
abstract::22 patients with untreated, essential hypertension (diastolic blood-pressure greater than or equal to 140 mmHg) and in most cases also showing neurological symptoms were given a single oral dose of 400 mg labetalol. All patients displayed a gradual decrease of the blood-pressure down to a diastolic pressure about 110 ...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009738709178680
更新日期:1987-01-01 00:00:00
abstract:AIM:To investigate how individuals fulfilling the Rome II criteria for irritable bowel syndrome (IBS) spontaneously described their symptoms. METHOD:From a general population, 1,244 randomly sampled adults were asked to describe their gastrointestinal symptoms (if any) verbally, in their own words, at a semi-structure...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009734.2015.1040529
更新日期:2015-01-01 00:00:00
abstract::Post-heparin plasma hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL) activities were 8-34 and 3.5-21 (range) mu mol/ml/hr respectively in males with normal serum lipid concentrations. In females the corresponding values were 4-25 and 4-16 mu mol/ml/hr. No significant differences were observed between a...
journal_title:Upsala journal of medical sciences
pub_type: 杂志文章
doi:10.3109/03009737909179158
更新日期:1979-01-01 00:00:00