A nicotinic acetylcholine receptor-like alpha-bungarotoxin-binding site on outer hair cells.

Abstract:

:Acetylcholine (ACh) appears to be the major neurotransmitter liberated from olivocochlear efferents terminating on outer hair cells (OHC). Recently, cholinergic receptor epitopes were visualized at the basal pole of the OHCs. To evaluate the ACh receptor type at OHC we performed binding studies with [125I]-labelled alpha-bungarotoxin (alpha-bgtx), a close to irreversibly acting blocker of the nicotinic acetylcholine receptor (nAChR) of skeletal muscle and of electrocytes of Torpedo and Electrophorus. An irreversible and saturable binding (80 nM) of the radiolabelled compound to OHCs was observed. The number of alpha-bgtx sensitive binding sites present on each OHC was calculated to be about 2 X 10(-17) mol/OHC, which would amount to about 10(7) binding sites/cell. Preincubation with the reversibly acting cholinergic ligands, carbamylcholine (1 mM), nicotine (0.1 mM) and d-tubocurarine (1-100 microM) was found to inhibit alpha-bgtx binding to a varying degree. Atropine (0.05 mM), a muscarinic antagonist, had no influence on the binding of alpha-bgtx to OHCs. [3H]-QNB, a specific marker and antagonist for muscarinic AChR, and [125I]-kappa-toxin, known to react with neuronal and ganglionic nAChR, showed no specific binding to OHCs. The data indicate that a peripheral type nAChR is present on OHCs mediating ACh-induced modulation of the biomechanics of the cochlea by influencing OHC motility.

journal_name

Hear Res

journal_title

Hearing research

authors

Plinkert PK,Zenner HP,Heilbronn E

doi

10.1016/0378-5955(91)90219-y

subject

Has Abstract

pub_date

1991-05-01 00:00:00

pages

123-30

issue

1

eissn

0378-5955

issn

1878-5891

pii

0378-5955(91)90219-Y

journal_volume

53

pub_type

杂志文章
  • Temporal modulation transfer functions measured from auditory-nerve responses following sensorineural hearing loss.

    abstract::The ability of auditory-nerve (AN) fibers to encode modulation frequencies, as characterized by temporal modulation transfer functions (TMTFs), generally shows a low-pass shape with a cut-off frequency that increases with fiber characteristic frequency (CF). Because AN-fiber bandwidth increases with CF, this result ha...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2012.02.004

    authors: Kale S,Heinz MG

    更新日期:2012-04-01 00:00:00

  • Magnitude and phase of three-dimensional (3D) velocity vector: Application to measurement of cochlear promontory motion during bone conduction sound transmission.

    abstract::Recent measurements of vibrational motion to assess sound transmission through ear structures and skull contents have included three-dimensional (3D) behavior. The 3D motion of a point has been described with the three orthogonal components in the 3D space. In this article, a method to represent the 3D velocity with t...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2018.03.022

    authors: Dobrev I,Sim JH

    更新日期:2018-07-01 00:00:00

  • Plastic changes along auditory pathway during salicylate-induced ototoxicity: Hyperactivity and CF shifts.

    abstract::High dose of salicylate, the active ingredient in aspirin, has long been known to induce transient hearing loss, tinnitus and hyperacusis making it a powerful experimental tool. These salicylate-induced perceptual disturbances are associated with a massive reduction in the neural output of the cochlea. Paradoxically, ...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2016.10.021

    authors: Jiang C,Luo B,Manohar S,Chen GD,Salvi R

    更新日期:2017-04-01 00:00:00

  • Responses of neurons in the feline inferior colliculus to modulated electrical stimuli applied on and within the ventral cochlear nucleus; Implications for an advanced auditory brainstem implant.

    abstract::Auditory brainstem implants (ABIs) can restore useful hearing to persons with deafness who cannot benefit from cochlear implants. However, the quality of hearing restored by ABIs rarely is comparable to that provided by cochlear implants in persons for whom those are appropriate. In an animal model, we evaluated eleme...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2018.03.009

    authors: McCreery D,Yadev K,Han M

    更新日期:2018-06-01 00:00:00

  • Exponential processes in human auditory excitation and adaptation.

    abstract::Peripheral auditory adaptation has been studied extensively in animal models, and multiple exponential components have been identified. This study explores the feasibility of estimating these component processes for human listeners with a peripheral model of adaptation. The processes were estimated from off-frequency ...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(01)00428-2

    authors: Formby C,Rutledge JC,Sherlock LP

    更新日期:2002-02-01 00:00:00

  • Masking by harmonic complexes in birds: behavioral thresholds and cochlear responses.

    abstract::Thresholds for pure tones embedded in harmonic complexes were measured behaviorally and physiologically for three species of birds, and physiologically in gerbils. The harmonic maskers were generated using the Schroeder-phase algorithm, characterized by monotonically increasing or decreasing phase across frequency. Pr...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(00)00249-5

    authors: Dooling RJ,Dent ML,Leek MR,Gleich O

    更新日期:2001-02-01 00:00:00

  • Increase in glutamate-aspartate transporter (GLAST) mRNA during kanamycin-induced cochlear insult in rats.

    abstract::Kanamycin (KM)-induced changes in expression of the gene for glutamate-aspartate transporter (GLAST) in the rat cochlea were analyzed by Northern blotting. With the administration of KM (600 mg/kg/day) once daily for 20 days, the expression of GLAST mRNA gradually increased and reached a peak on day 20. Although the e...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(99)00050-7

    authors: Matsuda K,Ueda Y,Doi T,Tono T,Haruta A,Toyama K,Komune S

    更新日期:1999-07-01 00:00:00

  • Endocochlear potential in focal lesions of the guinea pig cochlea.

    abstract::To estimate the correlation between the cochlear lateral wall and endocochlear potential (EP), the EP was measured at different time intervals up to 3 weeks at cochlear focal lesions made in the guinea pig. Lesions were produced by a photochemical reaction between systemically administered Rose Bengal and green light ...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(98)00204-4

    authors: Wu R,Hoshino T,Nagura M

    更新日期:1999-02-01 00:00:00

  • Neurophysiological model of tinnitus: dependence of the minimal masking level on treatment outcome.

    abstract::Validity of the neurophysiological model of tinnitus (Jastreboff, 1990), outlined in this paper, was tested on data from multicenter trial of tinnitus masking (Hazell et al., 1985). Minimal masking level, intensity match of tinnitus, and the threshold of hearing have been evaluated on a total of 382 patients before an...

    journal_title:Hearing research

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1016/0378-5955(94)90113-9

    authors: Jastreboff PJ,Hazell JW,Graham RL

    更新日期:1994-11-01 00:00:00

  • Oncomodulin is abundant in the organ of Corti.

    abstract::A small, acidic Ca(2+)-binding protein (CBP-15) was recently detected in extracts of the mammalian auditory receptor organ, the organ of Corti [Senarita et al. (1995) Hear. Res. 90, 169-175]. N-terminal sequence data for CBP-15 [Thalmann et al. (1995) Biochem. Biophys. Res. Commun. 215, 142-147] implied membership in ...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(97)00005-1

    authors: Henzl MT,Shibasaki O,Comegys TH,Thalmann I,Thalmann R

    更新日期:1997-04-01 00:00:00

  • Subtypes of GABAergic cells in the inferior colliculus.

    abstract::The inferior colliculus occupies a central position in ascending and descending auditory pathways. A substantial proportion of its neurons are GABAergic, and these neurons contribute to intracollicular circuits as well as to extrinsic projections to numerous targets. A variety of types of evidence - morphology, physio...

    journal_title:Hearing research

    pub_type: 杂志文章,评审

    doi:10.1016/j.heares.2018.10.001

    authors: Schofield BR,Beebe NL

    更新日期:2019-05-01 00:00:00

  • Morphological and morphometric characteristics of vestibular hair cells and support cells in long term cultures of rat utricle explants.

    abstract::A method for long term culture of utricular macula explants is demonstrated to be stable and reproducible over a period of 28 days in vitro (DIV). This culture system for four-day-old rat utricular maculae is potentially suitable for studies of hair cell loss, repair and regeneration processes as they occur in post-na...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2011.11.003

    authors: Werner M,Van De Water TR,Andersson T,Arnoldsson G,Berggren D

    更新日期:2012-01-01 00:00:00

  • The effects of moderate and low levels of acoustic overstimulation on stereocilia and their tip links in the guinea pig.

    abstract::Guinea pigs were exposed to pure tones of 10 kHz at intensities between 98 and 115 dB SPL for 5-30 min, to produce varying degrees of acoustic trauma. Changes in auditory thresholds were measured electrophysiologically, and the animals were immediately fixed for scanning electron microscopy. Correlation between morpho...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(96)00092-5

    authors: Clark JA,Pickles JO

    更新日期:1996-09-15 00:00:00

  • Functional organization of the forebrain auditory centres of the European starling: a study based on natural sounds.

    abstract::The field L complex is thought to be the highest auditory centre and the input in the song vocal nuclei. Different anatomical and functional subdivisions have been described in field L. Auditory neurons of field L are well activated by natural sounds and especially by species-specific sounds. A complex sound coding ap...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2005.01.008

    authors: Cousillas H,Leppelsack HJ,Leppelsack E,Richard JP,Mathelier M,Hausberger M

    更新日期:2005-09-01 00:00:00

  • Hyperpolarization-activated current (Ih) in primary auditory neurons.

    abstract::A hyperpolarization-activated current (termed I[h]) is believed to provide a pacemaker depolarization in sinoatrial node cells and in some central and peripheral neurons. In the present study, we examined if such an inward cation current exists in primary auditory neurons using the whole-cell patch-clamp technique. A ...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(97)00078-6

    authors: Chen C

    更新日期:1997-08-01 00:00:00

  • RNA analysis of inner ear cells from formalin fixed paraffin embedded (FFPE) archival human temporal bone section using laser microdissection--a technical report.

    abstract:OBJECTIVE:Molecular analysis using archival human inner ear specimens is challenging because of the anatomical complexity, long-term fixation, and decalcification. However, this method may provide great benefit for elucidation of otological diseases. Here, we extracted mRNA for RT-PCR from tissues dissected from archiv...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2013.04.008

    authors: Kimura Y,Kubo S,Koda H,Shigemoto K,Sawabe M,Kitamura K

    更新日期:2013-08-01 00:00:00

  • An active process in cochlear mechanics.

    abstract::A model for cochlear mechanics is proposed to take account of its two systems, one passive and one active. The classical passive system stimulates the inner hair cells directly at levels above about 40 dB SL. At intensities below about 60 dB an active process, the 'cochlear amplifier' (CA), somehow provides additional...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/0378-5955(83)90136-3

    authors: Davis H

    更新日期:1983-01-01 00:00:00

  • Selective attention reduces physiological noise in the external ear canals of humans. II: visual attention.

    abstract::Human subjects performed in several behavioral conditions requiring, or not requiring, selective attention to visual stimuli. Specifically, the attentional task was to recognize strings of digits that had been presented visually. A nonlinear version of the stimulus-frequency otoacoustic emission (SFOAE), called the nS...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2014.03.013

    authors: Walsh KP,Pasanen EG,McFadden D

    更新日期:2014-06-01 00:00:00

  • Turtle utricle dynamic behavior using a combined anatomically accurate model and experimentally measured hair bundle stiffness.

    abstract::Anatomically correct turtle utricle geometry was incorporated into two finite element models. The geometrically accurate model included appropriately shaped macular surface and otoconial layer, compact gel and column filament (or shear) layer thicknesses and thickness distributions. The first model included a shear la...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2014.10.010

    authors: Davis JL,Grant JW

    更新日期:2014-12-01 00:00:00

  • A comparison of auditory evoked potentials to acoustic beats and to binaural beats.

    abstract::The purpose of this study was to compare cortical brain responses evoked by amplitude modulated acoustic beats of 3 and 6 Hz in tones of 250 and 1000 Hz with those evoked by their binaural beats counterparts in unmodulated tones to indicate whether the cortical processes involved differ. Event-related potentials (ERPs...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2010.01.013

    authors: Pratt H,Starr A,Michalewski HJ,Dimitrijevic A,Bleich N,Mittelman N

    更新日期:2010-04-01 00:00:00

  • Tectorial membrane regeneration in acoustically damaged birds: an immunocytochemical technique.

    abstract::A novel immunocytochemical method was used to determine whether the sound-damaged adult quail ear can repair its tectorial membrane (TM) and to compare the repair in quail to that in chicks. Birds were exposed to an octave band noise with a center frequency of 1.5 kHz at 116 dB SPL for 4 h. The chicks were grouped bas...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/0378-5955(95)00051-5

    authors: Adler HJ,Niemiec AJ,Moody DB,Raphael Y

    更新日期:1995-06-01 00:00:00

  • Simulation of ILD sensitive neurons in the inferior colliculus of the barn owl.

    abstract::The barn owl (Tyto alba) uses interaural level difference (ILD) as a cue for the localization of sound. The first site of binaural convergence in the pathway that processes ILD is the ventral lateral lemniscus pars posterior (VLVp). Neurons in VLVp receive excitatory input from the contralateral nucleus angularis, and...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(98)00102-6

    authors: Peddicord R

    更新日期:1998-09-01 00:00:00

  • Plasticity of somatosensory inputs to the cochlear nucleus--implications for tinnitus.

    abstract::This chapter reviews evidence for functional connections of the somatosensory and auditory systems at the very lowest levels of the nervous system. Neural inputs from the dosal root and trigeminal ganglia, as well as their brain stem nuclei, cuneate, gracillis and trigeminal, terminate in the cochlear nuclei. Terminat...

    journal_title:Hearing research

    pub_type: 杂志文章,评审

    doi:10.1016/j.heares.2011.05.001

    authors: Shore SE

    更新日期:2011-11-01 00:00:00

  • CM tuning can be compatible with sharply tuned receptor potentials.

    abstract::There is convincing evidence that cochlear microphonics (CM) arise primarily from outer hair cells and have a frequency distribution that is much broader than that measured in inner hair cells by Russel and Sellick [6,7]. The broad tuning results from the fact that CM generated at each location decays exponentially al...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/0378-5955(80)90092-1

    authors: Kletsky EJ,Zwislocki JJ

    更新日期:1980-06-01 00:00:00

  • The frequency-following response (FFR) to speech stimuli: A normative dataset in healthy newborns.

    abstract::The Frequency-Following Response (FFR) is a neurophonic auditory evoked potential that reflects the efficient encoding of speech sounds and is disrupted in a range of speech and language disorders. This raises the possibility to use it as a potential biomarker for literacy impairment. However, reference values for com...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2018.11.001

    authors: Ribas-Prats T,Almeida L,Costa-Faidella J,Plana M,Corral MJ,Gómez-Roig MD,Escera C

    更新日期:2019-01-01 00:00:00

  • Auditory evoked-potential correlates of decrement detection.

    abstract::Decrement detection is a commonly used psychophysical technique in which a subject is required to detect partially filled gaps in an ongoing sound. The paradigm provides information regarding both temporal resolution and intensity discrimination. The purpose of this project was to determine if an evoked-potential para...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2005.10.011

    authors: Boettcher FA,Emery M

    更新日期:2006-02-01 00:00:00

  • Contribution of stapedial artery to blood flow in the cochlea and its surrounding bone.

    abstract::This study was performed to elucidate the contribution of the stapedial artery (SA)--which has been considered to be independent of the vertebrobasilar system--to blood flow in the ear by observing ear blood flow in Sprague-Dawley rats. A laser-Doppler (LD) probe was positioned on the bony wall of the cochlear basal t...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(03)00310-1

    authors: Yamamoto H,Tominaga M,Sone M,Nakashima T

    更新日期:2003-12-01 00:00:00

  • Cochlear-implant spatial selectivity with monopolar, bipolar and tripolar stimulation.

    abstract::Sharp spatial selectivity is critical to auditory performance, particularly in pitch-related tasks. Most contemporary cochlear implants have employed monopolar stimulation that produces broad electric fields, which presumably contribute to poor pitch and pitch-related performance by implant users. Bipolar or tripolar ...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2011.11.005

    authors: Zhu Z,Tang Q,Zeng FG,Guan T,Ye D

    更新日期:2012-01-01 00:00:00

  • Expanding the biological basis of tinnitus: crossmodal origins and the role of neuroplasticity.

    abstract::Tinnitus is most often initiated by modality specific otopathologic disturbances affecting peripheral and central auditory pathways. However, there is growing evidence indicating that the anatomical location generating tinnitus occurs at sites different from the initial pathology. Support for this notion is found in i...

    journal_title:Hearing research

    pub_type: 杂志文章,评审

    doi:10.1016/s0378-5955(02)00717-7

    authors: Cacace AT

    更新日期:2003-01-01 00:00:00

  • Computer simulation of the mechanical stimulation of the saccular membrane of bullfrog.

    abstract::A three-dimensional computer simulation of the experiment (Benser M. E., Issa N.P., Hudspeth A.J., 1993. Hear. Res. 68, 243-252), devoted to the mechanical stimulation of the surface of saccular membrane of bullfrog with the otoconial mass removed was carried out by finite-element method. Comparison of the calculated ...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(00)00034-4

    authors: Kondrachuk AV

    更新日期:2000-05-01 00:00:00