Abstract:
:The delivery of AMPA receptors to the plasma membrane is a critical step both for the synaptic delivery of these receptors and for the regulation of synaptic transmission. To directly visualize fusion events of transport vesicles containing the AMPA receptor GluA2 subunit with the plasma membrane we used pHluorin-tagged GluA2 subunits and total internal reflection fluorescence microscopy. We demonstrate that the plasma membrane insertion of GluA2 requires the NSF binding site within its intracellular cytoplasmic domain and that RNA editing of the Q/R site in the ion channel region plays a key role in GluA2 plasma membrane insertion. Finally, we show that plasma membrane insertion of heteromeric GluA2/3 receptors follows the same rules as homomeric GluA2 receptors. These results demonstrate that the plasma membrane delivery of GluA2 containing AMPA receptors is regulated by its unique structural elements.
journal_name
Proc Natl Acad Sci U S Aauthors
Araki Y,Lin DT,Huganir RLdoi
10.1073/pnas.1006584107subject
Has Abstractpub_date
2010-06-15 00:00:00pages
11080-5issue
24eissn
0027-8424issn
1091-6490pii
1006584107journal_volume
107pub_type
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