Isolation of a yeast artificial chromosome spanning the 8;21 translocation breakpoint t(8;21)(q22;q22.3) in acute myelogenous leukemia.

abstract：:The suprachiasmatic nucleus (SCN), the master circadian clock in mammals, sends major output signals to the subparaventricular zone (SPZ) and further to the paraventricular nucleus (PVN), the neural mechanism of which is largely unknown. In this study, the intracellular calcium levels were measured continuously in cul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu YE,Enoki R,Oda Y,Huang ZL,Honma KI,Honma S

更新日期：2018-10-02 00:00:00

abstract：:The surface density of the triggering receptors responsible for the natural killer (NK)-mediated cytotoxicity is crucial for the ability of NK cells to kill susceptible target cells. In this study, we show that transforming growth factor beta1 (TGFbeta1) down-regulates the surface expression of NKp30 and in part of NK...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castriconi R,Cantoni C,Della Chiesa M,Vitale M,Marcenaro E,Conte R,Biassoni R,Bottino C,Moretta L,Moretta A

更新日期：2003-04-01 00:00:00

abstract：:Standard economic and evolutionary models assume that humans are fundamentally selfish. On this view, any acts of prosociality--such as cooperation, giving, and other forms of altruism--result from covert attempts to avoid social injunctions against selfishness. However, even in the absence of social pressure, individ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zaki J,Mitchell JP

更新日期：2011-12-06 00:00:00

abstract：:We present a method for studying multiple retroviral integration events into a small DNA target in vivo. Episomal simian virus 40 (SV40) genomes established by infection of CV-1 cells served as integration targets during subsequent infection with murine leukemia virus (MLV). Using a PCR-based assay for the abundance a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pryciak PM,Müller HP,Varmus HE

更新日期：1992-10-01 00:00:00

abstract：:The plant aquaporin plasma membrane intrinsic proteins (PIP) subfamily represents one of the main gateways for water exchange at the plasma membrane (PM). A fraction of this subfamily, known as PIP1, does not reach the PM unless they are coexpressed with a PIP2 aquaporin. Although ubiquitous and abundantly expressed, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yaneff A,Sigaut L,Marquez M,Alleva K,Pietrasanta LI,Amodeo G

更新日期：2014-01-07 00:00:00

abstract：:Biotin carboxylase [biotin-carboxyl-carrier-protein:carbon-dioxide ligase (ADP-forming), EC 6.3.4.14] is the enzyme mediating the first step of the acetyl-CoA carboxylase [acetyl-CoA:carbon-dioxide ligase (ADP-forming), EC 6.4.1.2] reaction. We screened an Escherichia coli DNA library and a DNA fragment carrying the b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kondo H,Shiratsuchi K,Yoshimoto T,Masuda T,Kitazono A,Tsuru D,Anai M,Sekiguchi M,Tanabe T

更新日期：1991-11-01 00:00:00

abstract：:KcsA is a prokaryotic pH-dependent potassium (K) channel. Its activation, by a decrease in the intracellular pH, is coupled with its subsequent inactivation, but the underlying mechanisms remain elusive. Here, we have investigated the conformational changes and equilibrium of KcsA by using solution NMR spectroscopy. C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Imai S,Osawa M,Takeuchi K,Shimada I

更新日期：2010-04-06 00:00:00

abstract：:The conservation of developmental functions exerted by Antp-class homeoproteins in protostomes and deuterostomes suggested that homologs with related functions are present in diploblastic animals. Our phylogenetic analyses showed that Antp-class homeodomains belong either to non-Hox or to Hox/paraHox families. Among t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gauchat D,Mazet F,Berney C,Schummer M,Kreger S,Pawlowski J,Galliot B

更新日期：2000-04-25 00:00:00

abstract：:Efficient 3'-end processing of cell cycle-regulated mammalian histone premessenger RNAs (pre-mRNAs) requires an upstream stem-loop and a histone downstream element (HDE) that base pairs with the U7 small ribonucleoprotein. Insertions between these elements have two effects: the site of cleavage moves in concert with t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scharl EC,Steitz JA

更新日期：1996-12-10 00:00:00

abstract：:Fast axoplasmic transport was studied in dorsal root ganglion cells of the cat. Proteins carried in the fast axoplasmic flow were labeled after an intraganglionic injection of (L)-[4,5-(3)H]leucine. The rate of transport was 380 +/- 26 mm/day in both the central and peripheral branches of the bifurcating axons that ar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson LE,McClure WO

更新日期：1973-05-01 00:00:00

abstract：:The neuropeptides oxytocin and vasopressin are evolutionarily conserved regulators of social perception and behavior. Evidence is building that they are critically involved in the development of social recognition skills within rodent species, primates, and humans. We investigated whether common polymorphisms in the g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skuse DH,Lori A,Cubells JF,Lee I,Conneely KN,Puura K,Lehtimäki T,Binder EB,Young LJ

更新日期：2014-02-04 00:00:00

abstract：:Genetic deletion studies have shown that haploinsufficiency of Delta-like ligand (Dll) 4, a transmembrane ligand for the Notch family of receptors, results in major vascular defects and embryonic lethality. To better define the role of Dll4 during vascular growth and differentiation, we selected the postnatal retina a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lobov IB,Renard RA,Papadopoulos N,Gale NW,Thurston G,Yancopoulos GD,Wiegand SJ

更新日期：2007-02-27 00:00:00

abstract：:A vaccinia virus host range mutant (hr mutant) deleted of 18 kilobase pairs at the left end of the genome has been employed to precisely map a viral gene required for multiplication in human cells. DNA fragments from the wild-type virus were inserted into the thymidine kinase gene of mutant virus by means of in vivo h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gillard S,Spehner D,Drillien R,Kirn A

更新日期：1986-08-01 00:00:00

abstract：:To understand the basis for T-cell-specific induction of interleukin 2 (IL-2), we have analyzed nuclear factors from the Jurkat T-lymphoid leukemia cell, which can be induced to secrete IL-2. We have used an electrophoretic mobility shift assay to examine binding of proteins to the upstream regulatory region, before a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nabel GJ,Gorka C,Baltimore D

更新日期：1988-05-01 00:00:00

abstract：:Synechococcus elongatus PCC 7942 is a genetically tractable model cyanobacterium that has been engineered to produce industrially relevant biomolecules and is the best-studied model for a prokaryotic circadian clock. However, the organism is commonly grown in continuous light in the laboratory, and data on metabolic p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Diamond S,Jun D,Rubin BE,Golden SS

更新日期：2015-04-14 00:00:00

abstract：:The mechanisms underlying analgesia related to social touch are not clear. While recent research highlights the role of the empathy of the observer to pain relief in the target, the contribution of social interaction to analgesia is unknown. The current study examines brain-to-brain coupling during pain with interpers...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldstein P,Weissman-Fogel I,Dumas G,Shamay-Tsoory SG

更新日期：2018-03-13 00:00:00

abstract：:The chemotherapeutic drug Taxol is known to interact within a specific site on beta-tubulin. Although the general location of the site has been defined by photoaffinity labeling and electron crystallography, the original data were insufficient to make an absolute determination of the bound conformation. We have now co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Snyder JP,Nettles JH,Cornett B,Downing KH,Nogales E

更新日期：2001-04-24 00:00:00

abstract：:RNA interference, mediated by small interfering RNAs (siRNAs), is a conserved regulatory process that has evolved as an antiviral defense mechanism in plants and animals. It is not known whether host cells also use siRNAs as an antibacterial defense mechanism in eukaryotes. Here, we report the discovery of an endogeno...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Katiyar-Agarwal S,Morgan R,Dahlbeck D,Borsani O,Villegas A Jr,Zhu JK,Staskawicz BJ,Jin H

更新日期：2006-11-21 00:00:00

abstract：:A subpopulation of phenobarbital-induced cytochrome P-450 in rat liver has been shown to consist of four closely related forms of the enzyme that appeared to be strain-related. In the present study, polypeptides composing this family were analyzed by two-dimensional electrophoresis of hepatic microsomes from 64 indivi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rampersaud A,Walz FG Jr

更新日期：1983-11-01 00:00:00

abstract：:Mutagenesis of protein-encoding sequences occurs ubiquitously; it enables evolution, accumulates during aging, and is associated with disease. Many biotechnological methods exploit random mutations to evolve novel proteins. To quantitate protein tolerance to random change, it is vital to understand the probability tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo HH,Choe J,Loeb LA

更新日期：2004-06-22 00:00:00

abstract：:Some trypanosomatid protozoa (order Kinetoplastida) are well known to harbor bacterial endosymbionts. Their phylogenetic positions and evolutionary relationships with the hosts were deduced by comparing the rRNA gene sequences. Earlier, we observed that these symbionts from three Crithidia spp. are identical and are c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Du Y,Maslov DA,Chang KP

更新日期：1994-08-30 00:00:00

abstract：:Protein phosphatase 2C (PP2C) is a class of ubiquitous and evolutionarily conserved serine/threonine PP involved in stress responses in yeasts, mammals, and plants. Here, I present mutational analysis of two Arabidopsis thaliana PP2Cs, encoded by ABI1 and AtPP2C, involved in the plant stress hormone abscisic acid (ABA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sheen J

更新日期：1998-02-03 00:00:00

abstract：:Tritiated photoaffinity analogs of the natural lepidopteran juvenile hormones, JH I and II [epoxy[3H]bishomofarnesyl diazoacetate ([3H]EBDA) and epoxy[3H]homofarnesyl diazoacetate ([3H]EHDA)], and of the JH analog methoprene [[3H]methoprene diazoketone ([3H]MDK)] were synthesized and used to identify specific JH bindi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Palli SR,Osir EO,Eng W,Boehm MF,Edwards M,Kulcsar P,Ujvary I,Hiruma K,Prestwich GD,Riddiford LM

更新日期：1990-01-01 00:00:00

abstract：:The activation of cyclin-dependent kinases (cdks) has been implicated in apoptosis induced by various stimuli. We find that the Fas-induced activation of cdc2 and cdk2 in Jurkat cells is not dependent on protein synthesis, which is shut down very early during apoptosis before caspase-3 activation. Instead, activation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou BB,Li H,Yuan J,Kirschner MW

更新日期：1998-06-09 00:00:00

abstract：:Antibodies prepared against peptides CP2, CP4, and CP5, which occur within the first 1522 amino acid residues of the alpha 1 subunit of dihydropyridine-sensitive skeletal muscle calcium channels, specifically recognized a 175-kDa form of the alpha 1 subunit in immunoblots and immunoprecipitation experiments. In contra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Jongh KS,Merrick DK,Catterall WA

更新日期：1989-11-01 00:00:00

abstract：:Although gamma/delta T cells express numerous in vitro functions similar to alpha/beta T cells, little is known about their biological functioning in vivo. Furthermore, it is unclear whether alpha/beta T cells and gamma/delta T cells act independently or in a coordinated way. In the present study, gamma/delta T cells ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaufmann SH,Blum C,Yamamoto S

更新日期：1993-10-15 00:00:00

abstract：:Dysregulation of cyclin-dependent kinase 5 (cdk5) per relative concentrations of its activators p35 and p25 is implicated in neurodegenerative diseases. P35 has a short t½ and undergoes rapid proteasomal degradation in its membrane-bound myristoylated form. P35 is converted by calpain to p25, which, along with an exte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsai SY,Pokrass MJ,Klauer NR,Nohara H,Su TP

更新日期：2015-05-26 00:00:00

abstract：:To investigate the evolution of the mammalian sex chromosomes, we have compared the gene content of the X chromosomes in the mammalian groups most distantly related to man (marsupials and monotremes). Previous work established that genes on the long arm of the human X chromosome are conserved on the X chromosomes in a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watson JM,Spencer JA,Riggs AD,Graves JA

更新日期：1991-12-15 00:00:00

abstract：:Understanding mammalian spermatozoan development and the events surrounding fertilization has grown slowly, in part because of uncertainty about the number and identity of the cellular components involved. Determination of those transcripts expressed specifically by germ cells should provide an inclusive list of proba...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schultz N,Hamra FK,Garbers DL

更新日期：2003-10-14 00:00:00

abstract：:We report the isolation and functional characterization of cDNAs encoding a Drosophila kainate-selective glutamate receptor. The deduced mature 964-residue protein (DGluR-I) is 108,482 Da and exhibits significant homology to mammalian glutamate receptor subunits. Injection of DGluR-I cRNA into Xenopus oocytes generate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ultsch A,Schuster CM,Laube B,Schloss P,Schmitt B,Betz H

更新日期：1992-11-01 00:00:00