Probing cytoplasmic organization and the actin cytoskeleton of plant cells with optical tweezers.

Abstract:

:In interphase plant cells, the actin cytoskeleton is essential for intracellular transport and organization. To fully understand how the actin cytoskeleton functions as the structural basis for cytoplasmic organization, both molecular and physical aspects of the actin organization have to be considered. In the present review, we discuss literature that gives an insight into how cytoplasmic organization is achieved and in which actin-binding proteins have been identified that play a role in this process. We discuss how physical properties of the actin cytoskeleton in the cytoplasm of live plant cells, such as deformability and elasticity, can be probed by using optical tweezers. This technique allows non-invasive manipulation of cytoplasmic organization. Optical tweezers, integrated in a confocal microscope, can be used to manipulate cytoplasmic organization while studying actin dynamics. By combining this with mutant studies and drug applications, insight can be obtained about how the physical properties of the actin cytoskeleton, and thus the cytoplasmic organization, are influenced by different cellular processes.

journal_name

Biochem Soc Trans

authors

Ketelaar T,van der Honing HS,Emons AM

doi

10.1042/BST0380823

subject

Has Abstract

pub_date

2010-06-01 00:00:00

pages

823-8

issue

3

eissn

0300-5127

issn

1470-8752

pii

BST0380823

journal_volume

38

pub_type

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