Abstract:
AIMS:To assess the potential of electrospun poly(lactide-co-glycolide) membranes to provide a biodegradable cell carrier system for limbal epithelial cells. MATERIAL & METHODS:50:50 poly(lactide-co-glycolide) scaffolds were spun, sterilized and seeded with primary rabbit limbal epithelial cells. Cells were cultured on the scaffolds for 2 weeks and then examined by confocal microscopy, cryosectioning and scanning-electron microscopy. The tensile strength of scaffolds before and after annealing and sterilization was also studied. RESULTS:The limbal cells had formed a continuous multilayer of cells on either side of the scaffold. Scaffolds with cells showed signs of the onset of degradation within 2 weeks in culture media at 37 degrees C. Scaffolds that were annealed resulted in a more brittle and stiff mat. CONCLUSIONS:We suggest this carrier membrane could be used as a replacement for the human amniotic membrane in the treatment of limbal stem cell deficiency, lowering the risk of disease transmission to the patient.
journal_name
Regen Medjournal_title
Regenerative medicineauthors
Deshpande P,McKean R,Blackwood KA,Senior RA,Ogunbanjo A,Ryan AJ,MacNeil Sdoi
10.2217/rme.10.16subject
Has Abstractpub_date
2010-05-01 00:00:00pages
395-401issue
3eissn
1746-0751issn
1746-076Xjournal_volume
5pub_type
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