Abstract:
:Three normalized cDNA libraries were constructed, two of which were constructed from reproductive tissues ovary and testis, and the other one from pooled immune tissues including head kidney, intestine, liver and spleen. A total of 10 542 clones were sequenced generating 10 128 expressed sequence tags (ESTs). Cluster analysis indicated a total of 5808 unique sequences including 1712 contigs and 4096 singletons. A total of 4249 (73%) of the unique ESTs had significant hits to the non-redundant protein database, 2253 of which were annotated using Gene Ontology (GO) terms. A total of 311 microsatellites (with 246 having sufficient flanking sequences for primer design) and 6294 putative SNPs were identified. These genome resources provide the material basis for future microarray development, marker validation and genetic linkage and QTL analysis.
journal_name
J Fish Bioljournal_title
Journal of fish biologyauthors
Sha Z,Wang S,Zhuang Z,Wang Q,Wang Q,Li P,Ding H,Wang N,Liu Z,Chen Sdoi
10.1111/j.1095-8649.2010.02592.xsubject
Has Abstractpub_date
2010-04-01 00:00:00pages
1190-204issue
5eissn
0022-1112issn
1095-8649pii
JFB2592journal_volume
76pub_type
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abstract::Latitudinal variation in egg size and number in anadromous masu salmon Oncorhynchus masou was examined. Relatively greater variation in egg size occurred among rivers than among females within rivers or within females. Egg size was generally greater and egg number generally lower at more northerly latitudes. ...
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journal_title:Journal of fish biology
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journal_title:Journal of fish biology
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更新日期:2014-09-01 00:00:00
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journal_title:Journal of fish biology
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abstract::The pencil cardinal Epigonus denticulatus is a small deep-water fish inhabiting continental slopes usually between 300 m and 600 m depth. We report the first record of E. denticulatus in the Azores archipelago, where one specimen was found floating by fisherman off Faial island. Meristic and morphometric characters ar...
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pub_type: 杂志文章
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journal_title:Journal of fish biology
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更新日期:2018-10-01 00:00:00