Generation and analysis of 10 000 ESTs from the half-smooth tongue sole Cynoglossus semilaevis and identification of microsatellite and SNP markers.

Abstract:

:Three normalized cDNA libraries were constructed, two of which were constructed from reproductive tissues ovary and testis, and the other one from pooled immune tissues including head kidney, intestine, liver and spleen. A total of 10 542 clones were sequenced generating 10 128 expressed sequence tags (ESTs). Cluster analysis indicated a total of 5808 unique sequences including 1712 contigs and 4096 singletons. A total of 4249 (73%) of the unique ESTs had significant hits to the non-redundant protein database, 2253 of which were annotated using Gene Ontology (GO) terms. A total of 311 microsatellites (with 246 having sufficient flanking sequences for primer design) and 6294 putative SNPs were identified. These genome resources provide the material basis for future microarray development, marker validation and genetic linkage and QTL analysis.

journal_name

J Fish Biol

journal_title

Journal of fish biology

authors

Sha Z,Wang S,Zhuang Z,Wang Q,Wang Q,Li P,Ding H,Wang N,Liu Z,Chen S

doi

10.1111/j.1095-8649.2010.02592.x

subject

Has Abstract

pub_date

2010-04-01 00:00:00

pages

1190-204

issue

5

eissn

0022-1112

issn

1095-8649

pii

JFB2592

journal_volume

76

pub_type

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