Abstract:
BACKGROUND:Prostaglandin E2 (PGE2) is involved in several chronic inflammatory diseases including periodontitis, which causes loss of the gingival tissue and alveolar bone supporting the teeth. We have previously shown that tumor necrosis factor alpha (TNFalpha) induces PGE2 synthesis in gingival fibroblasts. In this study we aimed to investigate the global gene expression profile of TNFalpha-stimulated primary human gingival fibroblasts, focusing on signal pathways related to the PGE2-synthesizing enzymes prostaglandin E synthases (PGES), as well as the upstream enzyme cyclooxygenase-2 (COX-2) and PGE2 production. RESULTS:Microarray and western blot analyses showed that the mRNA and protein expression of the inflammatory induced microsomal prostaglandin E synthase-1 (mPGES-1) was up-regulated by the cytokine TNFalpha, accompanied by enhanced expression of COX-2 and increased production of PGE2. In contrast, the expression of the isoenzymes microsomal prostaglandin E synthase-2 (mPGES-2) and cytosolic prostaglandin E synthase (cPGES) was unaffected by TNFalpha treatment. Using oligonucleotide microarray analysis in a time-course factorial design including time points 1, 3 and 6 h, differentially expressed genes in response to TNFalpha treatment were identified. Enrichment analysis of microarray data indicated two positively regulated signal transduction pathways: c-Jun N-terminal kinase (JNK) and Nuclear Factor-kappaB (NF-kappaB). To evaluate their involvement in the regulation of mPGES-1 and COX-2 expression, we used specific inhibitors as well as phosphorylation analysis. Phosphorylation analysis of JNK (T183/Y185) and NF-kappaB p65 (S536) showed increased phosphorylation in response to TNFalpha treatment, which was decreased by specific inhibitors of JNK (SP600125) and NF-kappaB (Bay 11-7082, Ro 106-9920). Inhibitors of JNK and NF-kappaB also decreased the TNFalpha-stimulated up-regulation of mPGES-1 and COX-2 as well as PGE2 production. CONCLUSION:In the global gene expression profile, the enrichment analysis of microarray data identified the two signal transduction pathways JNK and NF-kappaB as positively regulated by the cytokine TNFalpha. Inhibition of these TNFalpha-activated signal pathways reduced the expression of mPGES-1 and COX-2 as well as their end product PGE2 in gingival fibroblasts. The involvement of the signal pathways JNK and NF-kappaB in the regulation of PGE2 induced by TNFalpha may suggest these two pathways as possible attractive targets in the chronic inflammatory disease periodontitis.
journal_name
BMC Genomicsjournal_title
BMC genomicsauthors
Båge T,Lindberg J,Lundeberg J,Modéer T,Yucel-Lindberg Tdoi
10.1186/1471-2164-11-241subject
Has Abstractpub_date
2010-04-15 00:00:00pages
241issn
1471-2164pii
1471-2164-11-241journal_volume
11pub_type
杂志文章相关文献
BMC GENOMICS文献大全abstract:BACKGROUND:Protein structure comparison and classification is an effective method for exploring protein structure-function relations. This problem is computationally challenging. Many different computational approaches for protein structure comparison apply the secondary structure elements (SSEs) representation of prot...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-S2-S1
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Two fifths of the world's population is at risk from dengue. The absence of effective drugs and vaccines leaves vector control as the primary intervention tool. Understanding dengue virus (DENV) host interactions is essential for the development of novel control strategies. The availability of genome sequenc...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-380
更新日期:2010-06-16 00:00:00
abstract:BACKGROUND:The food spoilage yeast species Zygosaccharomyces bailii exhibits an extraordinary capacity to tolerate weak acids, in particular acetic acid. In Saccharomyces cerevisiae, the transcription factor Haa1 (ScHaa1) is considered the main player in genomic expression reprogramming in response to acetic acid stres...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-3443-2
更新日期:2017-01-13 00:00:00
abstract:BACKGROUND:Nuclear receptors are hormone-regulated transcription factors whose signaling controls numerous aspects of development and physiology. Many receptors recognize DNA hormone response elements formed by direct repeats of RGKTCA motifs separated by 1 to 5 bp (DR1-DR5). Although many known such response elements ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-8-23
更新日期:2007-01-19 00:00:00
abstract:BACKGROUND:Intensive selection has resulted in increased growth rates and muscularity in broiler chickens, in addition to adverse effects, including delayed organ development, sudden death syndrome, and altered metabolic rates. The biological mechanisms underlying selection responses remain largely unknown. Non-artific...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-3845-9
更新日期:2017-06-20 00:00:00
abstract:BACKGROUND:Gaucher disease is caused by defective glucocerebrosidase activity and the consequent accumulation of glucosylceramide. The pathogenic pathways resulting from lipid laden macrophages (Gaucher cells) in visceral organs and their abnormal functions are obscure. RESULTS:To elucidate this pathogenic pathway, de...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-12-20
更新日期:2011-01-11 00:00:00
abstract:BACKGROUND:Fungal plant pathogens belonging to the genus Venturia cause damaging scab diseases of members of the Rosaceae. In terms of economic impact, the most important of these are V. inaequalis, which infects apple, and V. pirina, which is a pathogen of European pear. Given that Venturia fungi colonise the sub-cuti...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-3699-1
更新日期:2017-05-02 00:00:00
abstract:BACKGROUND:The trypanosomatids Leishmania major, Trypanosoma brucei and Trypanosoma cruzi cause some of the most debilitating diseases of humankind: cutaneous leishmaniasis, African sleeping sickness, and Chagas disease. These protozoa possess complex life cycles that involve development in mammalian and insect hosts, ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-6-127
更新日期:2005-09-15 00:00:00
abstract:BACKGROUND:Azadirachtin A is a triterpenoid from neem tree exhibiting excellent activities against over 600 insect species in agriculture. The production of azadirachtin A depends on extraction from neem tissues, which is not an eco-friendly and sustainable process. The low yield and discontinuous supply of azadirachti...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-07124-6
更新日期:2020-10-28 00:00:00
abstract:BACKGROUND:Digital gene expression profiling was used to characterize the assembly of genes expressed in equine skeletal muscle and to identify the subset of genes that were differentially expressed following a ten-month period of exercise training. The study cohort comprised seven Thoroughbred racehorses from a single...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-398
更新日期:2010-06-23 00:00:00
abstract:BACKGROUND:Nucleosomes facilitate the packaging of the eukaryotic genome and modulate the access of regulators to DNA. A detailed description of the nucleosomal organization under different transcriptional programmes is essential to understand their contribution to genomic regulation. RESULTS:To visualize the dynamics...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-813
更新日期:2013-11-21 00:00:00
abstract:BACKGROUND:Elucidating gut microbiota among gallstone patients as well as the complex bacterial colonization of cholesterol gallstones may help in both the prediction and subsequent lowered risk of cholelithiasis. To this end, we studied the composition of bacterial communities of gut, bile, and gallstones from 29 gall...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-669
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:The genome of pathogenic Leptospira interrogans contains two chromosomes. Plasmids and prophages are known to play specific roles in gene transfer in bacteria and can potentially serve as efficient genetic tools in these organisms. Although plasmids and prophage remnants have recently been reported in Leptos...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-1321-y
更新日期:2015-02-15 00:00:00
abstract:BACKGROUND:Parasitic wasps constitute one of the largest group of venomous animals. Although some physiological effects of their venoms are well documented, relatively little is known at the molecular level on the protein composition of these secretions. To identify the majority of the venom proteins of the endoparasit...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-693
更新日期:2010-12-07 00:00:00
abstract:BACKGROUND:Quercetin is a polyphenol of great interest given its antioxidant activity and involvement in the immune response. Although quercetin has been well studied at the molecular level as a gene regulator and an activator of specific cellular pathways, not much attention has been given to its mechanism of action a...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-5966-9
更新日期:2019-07-23 00:00:00
abstract:BACKGROUND:The androgen receptor plays a critical role throughout the progression of prostate cancer and is an important drug target for this disease. While chromatin immunoprecipitation coupled with massively parallel sequencing (ChIP-Seq) is becoming an essential tool for studying transcription and chromatin modifica...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-355
更新日期:2012-07-31 00:00:00
abstract:BACKGROUND:Horizontal gene transfer has shaped the evolution of the ammonium transporter/ammonia permease gene family. Horizontal transfers of ammonium transporter/ammonia permease genes into the fungi include one transfer from archaea to the filamentous ascomycetes associated with the adaptive radiation of the leotiom...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-225
更新日期:2013-04-04 00:00:00
abstract:BACKGROUND:Whole-exome sequencing (WES) is rapidly evolving into a tool of choice for rapid, and inexpensive identification of molecular genetic lesions within targeted regions of the human genome. While biases in WES coverage of nucleotides in targeted regions are recognized, it is not well understood how repetition o...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-2107-y
更新日期:2015-11-25 00:00:00
abstract:BACKGROUND:Cultivated peanut (Arachis hypogaea) is an allotetraploid species whose ancestral genomes are most likely derived from the A-genome species, A. duranensis, and the B-genome species, A. ipaensis. The very recent (several millennia) evolutionary origin of A. hypogaea has imposed a bottleneck for allelic and ph...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-469
更新日期:2012-09-11 00:00:00
abstract:BACKGROUND:Cadmium (Cd) is a serious heavy metal (HM) soil pollutant. To alleviate or even eliminate HM pollution in soil, environmental-friendly methods are applied. One is that special plants are cultivated to absorb the HM in the contaminated soil. As an excellent economical plant with ornamental value and sound ada...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-6152-9
更新日期:2019-11-20 00:00:00
abstract:BACKGROUND:Human genetic variation produces the wide range of phenotypic differences that make us individual. However, little is known about the distribution of variation in the most conserved functional regions of the human genome. We examined whether different subsets of the conserved human genome have been subjected...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-10-614
更新日期:2009-12-16 00:00:00
abstract:BACKGROUND:NGS data contains many machine-induced errors. The most advanced methods for the error correction heavily depend on the selection of solid k-mers. A solid k-mer is a k-mer frequently occurring in NGS reads. The other k-mers are called weak k-mers. A solid k-mer does not likely contain errors, while a weak k-...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-5272-y
更新日期:2018-12-31 00:00:00
abstract:BACKGROUND:Bacterial vaginosis (BV) is an ecological disorder of the vaginal microbiota that affects millions of women annually, and is associated with numerous adverse health outcomes including pre-term birth and the acquisition of sexually transmitted infections. However, little is known about the overall structure a...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-488
更新日期:2010-09-07 00:00:00
abstract:BACKGROUND:Bacteroides thetaiotaomicron, a predominant member of the human gut microbiota, is characterized by its ability to utilize a wide variety of polysaccharides using the extensive saccharolytic machinery that is controlled by an expanded repertoire of transcription factors (TFs). The availability of genomic seq...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-873
更新日期:2013-12-12 00:00:00
abstract:BACKGROUND:Laboratory evolution is an important tool for developing robust yeast strains for bioethanol production since the biological basis behind combined tolerance requires complex alterations whose proper regulation is difficult to achieve by rational metabolic engineering. Previously, we reported on the evolved i...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-1737-4
更新日期:2015-07-09 00:00:00
abstract:BACKGROUND:Genomic approaches provide unique opportunities to study interactions of insects with their pathogens. We developed a cDNA microarray to analyze the gene transcription profile of the lepidopteran pest Spodoptera frugiperda in response to injection of the polydnavirus HdIV associated with the ichneumonid wasp...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-7-160
更新日期:2006-06-21 00:00:00
abstract:BACKGROUND:Cattle and other ruminants have evolved the ability to derive most of their metabolic energy requirement from otherwise indigestible plant matter through a symbiotic relationship with plant fibre degrading microbes within a specialised fermentation chamber, the rumen. The genetic changes underlying the evolu...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-8-75
更新日期:2007-03-15 00:00:00
abstract:BACKGROUND:Exosomes, endosome-derived membrane microvesicles, contain specific RNA transcripts that are thought to be involved in cell-cell communication. These RNA transcripts have great potential as disease biomarkers. To characterize exosomal RNA profiles systemically, we performed RNA sequencing analysis using thre...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-319
更新日期:2013-05-10 00:00:00
abstract:BACKGROUND:Malaria, caused by the parasitic protist Plasmodium falciparum, represents a major public health problem in the developing world. The P. falciparum genome has been sequenced, which provides new opportunities for the identification of novel drug targets. Eukaryotic protein kinases (ePKs) form a large family o...
journal_title:BMC genomics
pub_type: 杂志文章,评审
doi:10.1186/1471-2164-5-79
更新日期:2004-10-12 00:00:00
abstract:BACKGROUND:Common Pekin and Muscovy ducks and their intergeneric hinny and mule hybrids have different abilities for fatty liver production. RNA-Seq analyses from the liver of these different genetic types fed ad libitum or overfed would help to identify genes with different response to overfeeding between them. Howeve...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-07099-4
更新日期:2020-10-02 00:00:00