Pre-clinical studies of retrovirally transduced human chondrocytes expressing transforming growth factor-beta-1 (TG-C).

Abstract:

BACKGROUND AIMS:The aim was to evaluate cartilage regeneration in animal models involving induced knee joint damage. Through cell-mediated gene therapy methods, a cell mixture comprising a 3:1 ratio of genetically unmodified human chondrocytes and transforming growth factor beta-1 (TGF-beta1)-secreting human chondrocytes (TG-C), generated via retroviral transduction, resulted in successful cartilage proliferation in damaged regions. METHODS:Non-clinical toxicology assessments for efficacy, biodistribution and local/systemic toxicity of single intra-articular administration of the cell mixture in mice, rabbits and goats was conducted. RESULTS:Administration of the mixture was tolerated well in all of the species. There was evidence of cartilage proliferation in rabbits and goats. As an additional precautionary step, the efficacy of TGF-beta1 secretion in irradiated human chondrocytes was also demonstrated. CONCLUSIONS:Four studies in rabbits and goats demonstrated the safety and efficacy of TG-C following direct intra-articular administration in animal models involving induced knee joint damage. Based on these pre-clinical studies authorization has been received from the USA Food and Drug Administration (FDA) to proceed with an initial phase I clinical study of TG-C for degenerative arthritis.

journal_name

Cytotherapy

journal_title

Cytotherapy

authors

Noh MJ,Copeland RO,Yi Y,Choi KB,Meschter C,Hwang S,Lim CL,Yip V,Hyun JP,Lee HY,Lee KH

doi

10.3109/14653240903470639

subject

Has Abstract

pub_date

2010-05-01 00:00:00

pages

384-93

issue

3

eissn

1465-3249

issn

1477-2566

pii

S1465-3249(10)70400-X

journal_volume

12

pub_type

杂志文章
  • Reprogramming of mouse renal tubular epithelial cells to induced pluripotent stem cells.

    abstract::Kidney disease has reached epidemic proportions and is associated with high mortality and morbidity rates. Stem cell-based therapy may effectively treat kidney damage by cell transplantation. The breakthrough discovery using a combination of four transcription factors to reprogram genetically somatic cells into induce...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1016/j.jcyt.2013.01.008

    authors: Wang WW,Wang W,Jiang Y,Han GF,Lu S,Li G,Zhang J

    更新日期:2013-05-01 00:00:00

  • Bimodal ex vivo expansion of T cells from patients with head and neck squamous cell carcinoma: a prerequisite for adoptive cell transfer.

    abstract:BACKGROUND AIMS:Adoptive transfer of tumor-infiltrating lymphocytes (TIL) has proven effective in metastatic melanoma and should therefore be explored in other types of cancer. The aim of this study was to examine the feasibility of potentially expanding clinically relevant quantities of tumor-specific T-cell cultures ...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.3109/14653249.2011.563291

    authors: Junker N,Andersen MH,Wenandy L,Dombernowsky SL,Kiss K,Sørensen CH,Therkildsen MH,Von Buchwald C,Andersen E,Straten PT,Svane IM

    更新日期:2011-08-01 00:00:00

  • Addition of varicella zoster virus-specific T cells to cytomegalovirus, Epstein-Barr virus and adenovirus tri-specific T cells as adoptive immunotherapy in patients undergoing allogeneic hematopoietic stem cell transplantation.

    abstract:BACKGROUND AIMS:Virus-specific T-cell immunotherapy is emerging as a promising management strategy for virus infections in patients after hematopoietic stem cell transplant (HSCT). Here we present outcomes of 10 adult patients who received multi-virus-specific T cells prophylactically after HSCT. METHODS:Donor-derived...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1016/j.jcyt.2015.07.005

    authors: Ma CK,Blyth E,Clancy L,Simms R,Burgess J,Brown R,Deo S,Micklethwaite KP,Gottlieb DJ

    更新日期:2015-10-01 00:00:00

  • Cell therapy in critical limb ischemia: A comprehensive analysis of two cell therapy products.

    abstract:BACKGROUND:Cell therapy has been proposed as a salvage limb procedure in critical limb ischemia (CLI). In spite of the fact that clinical trials found some efficacy, the mechanism of action remains elusive. The objective of this study was to characterize two autologous cell therapy products (CTPs) obtained from patient...

    journal_title:Cytotherapy

    pub_type: 临床试验,杂志文章

    doi:10.1016/j.jcyt.2016.10.013

    authors: Tournois C,Pignon B,Sevestre MA,Al-Rifai R,Creuza V,Poitevin G,François C,Nguyen P

    更新日期:2017-02-01 00:00:00

  • Adipose-derived mesenchymal stromal cells improve hemodynamic function in pulmonary arterial hypertension: identification of microRNAs implicated in modulating endothelial function.

    abstract::Pulmonary arterial hypertension (PAH) is characterized by pulmonary arterial endothelial hyperproliferation and dysfunction. Restoration of endothelial function is a common goal of available treatments. In the present study, human adipose-derived mesenchymal stromal cells (ASCs) were co-cultured with monocrotaline pyr...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1016/j.jcyt.2019.02.011

    authors: Wang P,Zhang C,Li J,Luo L,Zhang S,Dong F,Tang Z,Ni S

    更新日期:2019-04-01 00:00:00

  • Safety and biodistribution study of bone marrow-derived mesenchymal stromal cells and mononuclear cells and the impact of the administration route in an intact porcine model.

    abstract:BACKGROUND AIMS:Bone marrow mononuclear cells (BM-MNCs) and bone marrow-derived mesenchymal stem stromal cells (BM-MSCs) could have therapeutic potential for numerous conditions, including ischemia-related injury. Cells transplanted intravascularly may become entrapped in the lungs, which potentially decreases their th...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1016/j.jcyt.2014.12.004

    authors: Mäkelä T,Takalo R,Arvola O,Haapanen H,Yannopoulos F,Blanco R,Ahvenjärvi L,Kiviluoma K,Kerkelä E,Nystedt J,Juvonen T,Lehenkari P

    更新日期:2015-04-01 00:00:00

  • Simplified process for the production of anti-CD19-CAR-engineered T cells.

    abstract:BACKGROUND AIMS:Adoptive immunotherapy with the use of chimeric antigen receptor (CAR)-engineered T cells specific for CD19 has shown promising results for the treatment of B-cell lymphomas and leukemia. This therapy involves the transduction of autologous T cells with a viral vector and the subsequent cell expansion. ...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1016/j.jcyt.2013.06.003

    authors: Tumaini B,Lee DW,Lin T,Castiello L,Stroncek DF,Mackall C,Wayne A,Sabatino M

    更新日期:2013-11-01 00:00:00

  • Safety and efficacy of granulocyte-colony-stimulating factor administration following autologous intramuscular implantation of bone marrow mononuclear cells: a randomized controlled trial in patients with advanced lower limb ischemia.

    abstract:BACKGROUND AIMS:The aim was to investigate the therapeutic effect of granulocyte-colony-stimulating factor (G-CSF) administration following implantation of autologous bone marrow mononuclear cells (BM MNC) for patients with lower limb ischemia. METHODS:The design was a randomized controlled trial. Fifteen patients wit...

    journal_title:Cytotherapy

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.3109/14653240903518163

    authors: Zafarghandi MR,Ravari H,Aghdami N,Namiri M,Moazzami K,Taghiabadi E,Fazel A,Pournasr B,Farrokhi A,Sharifian RA,Salimi J,Moini M,Baharvand H

    更新日期:2010-10-01 00:00:00

  • Factors modulating circulation of hematopoietic progenitor cells in cord blood and neonates.

    abstract:BACKGROUND:Hematopoietic progenitor cells (HPC) circulate at high levels at birth and disappear rapidly afterwards, but the underlying mechanism it is not known. The aim of this study was to assess circulating HPC in cord blood at different gestational ages and shortly after birth and concomitantly study the biologic m...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1080/14653240802499148

    authors: Gonzalez S,Amat L,Azqueta C,Madrigal JA,Laïlla JM,Garcia J,Querol S

    更新日期:2009-01-01 00:00:00

  • Mesenchymal stromal cells and immunomodulation: A gathering of regulatory immune cells.

    abstract::Because of their well-recognized immunomodulatory properties, mesenchymal stromal cells (MSCs) represent an attractive cell population for therapeutic purposes. In particular, there is growing interest in the use of MSCs as cellular immunotherapeutics for tolerance induction in allogeneic transplantations and the trea...

    journal_title:Cytotherapy

    pub_type: 杂志文章,评审

    doi:10.1016/j.jcyt.2015.10.011

    authors: Najar M,Raicevic G,Fayyad-Kazan H,Bron D,Toungouz M,Lagneaux L

    更新日期:2016-02-01 00:00:00

  • Cryopreserved mesenchymal stromal cell treatment is safe and feasible for severe dilated ischemic cardiomyopathy.

    abstract:BACKGROUND AIMS:Bone marrow (BM) mesenchymal stromal cells (MSC) represent a novel therapy for severe heart failure with extensive myocardial scarring, especially when performed concurrently with conventional revascularization. However, stem cells are difficult to transport in culture media without risk of contaminatio...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.3109/14653240903313966

    authors: Chin SP,Poey AC,Wong CY,Chang SK,Teh W,Mohr TJ,Cheong SK

    更新日期:2010-01-01 00:00:00

  • Blood and marrow transplantation activities among adult patients in Turkey.

    abstract:BACKGROUND:Since 1978, 856 hematopoietic stem-cell transplantations have been performed for adult patients in Turkey. METHODS AND RESULTS:Data were collected on the source of hematopoietic progenitor cells, recipient diagnosis, conditioning regimen, DFS and mortality. The data were analyzed by the national registry sy...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1080/146532400539288

    authors: Blood and Marrow Transplantation Subcommittee, Turkish Societ of Hematology.

    更新日期:2000-01-01 00:00:00

  • Hyperbaric oxygen treatment effects on in vitro cultured umbilical cord blood CD34+ cells.

    abstract:BACKGROUND AIMS:Umbilical cord blood (UCB) provides an alternative source for hematopoietic stem/progenitor cells (HSPCs) in the treatment of hematological malignancies. However, clinical usage is limited due to the low quantity of HSPCs in each unit of cord blood and defects in bone marrow homing. Hyperbaric oxygen (H...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1016/j.jcyt.2017.08.020

    authors: Cheung KY,Berry A,Li D,Aljitawi OS

    更新日期:2018-01-01 00:00:00

  • Number of megakaryocytic progenitors and adhesion molecule expression of stem cells predict platelet engraftment after allogeneic hematopoietic stem cell transplantation.

    abstract:BACKGROUND:The mechanism of platelet recovery after hematopoietic stem cell transplantation and the factors that influence its time-course are not fully understood. Rapid hematopoietic recovery results in a reduction of transplantation-related complications. In the present study, we questioned and analyzed whether ther...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1080/14653240701561337

    authors: Woo KS,Goh RY,Kim SH,Kwon HC,Kim HJ,Lee YH,Han JY

    更新日期:2007-01-01 00:00:00

  • Recombinant expression of coagulation factor VIII in hepatic and non-hepatic cell lines stably transduced with third generation lentiviral vectors comprising the minimal factor VIII promoter.

    abstract:BACKGROUND:Lentiviral vectors have the capacity to transduce stably non-dividing, differentiated and undifferentiated cells of various tissues, including liver. To obtain high-level expression of transgenes, vectors often rely on viral promoters. However, recent data suggest that the supraphysiologic expression from ub...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1080/14653240701656053

    authors: Picanço V,Heinz S,Bott D,Behrmann M,Covas DT,Seifried E,Tonn T

    更新日期:2007-01-01 00:00:00

  • Porcine acellular dermal matrix accelerates wound healing through miR-124-3p.1 and miR-139-5p.

    abstract:BACKGROUND AIMS:Cutaneous wound management is a major health problem and imposes a huge economic burden worldwide. Previous studies have demonstrated that wound healing is a highly coordinated process including epithelialization, angiogenesis, remodeling and scarring. This progression requires self-renewal, preservatio...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1016/j.jcyt.2020.04.042

    authors: Chen X,Yang R,Wang J,Ruan S,Lin Z,Xin Q,Yang R,Xie J

    更新日期:2020-09-01 00:00:00

  • Good manufacturing practice-compliant validation and preparation of BM cells for the therapy of acute myocardial infarction.

    abstract:BACKGROUND:Intracoronary application of BM-derived cells for the treatment of acute myocardial infarction (AMI) is currently being studied intensively. Simultaneously, strict legal requirements surround the production of cells for clinical studies. Thus good manufacturing practice (GMP)-compliant collection and prepara...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1080/14653240601052734

    authors: Griesel C,Heuft HG,Herrmann D,Franke A,Ladas D,Stiehler N,Stucki A,Braun M,Wollert KC,Meyer GP,Drexler H,Hertenstein B,Ganser A,Rüdinger W,Arseniev L

    更新日期:2007-01-01 00:00:00

  • Ex vivo expansion of umbilical cord blood.

    abstract::The efficacy of cord blood (CB) transplantation is limited by the low cell dose available. Low cell doses at transplant are correlated with delayed engraftment, prolonged neutropenia and thrombocytopenia and elevated risk of graft failure. To potentially improve the efficacy of CB transplantation, approaches have been...

    journal_title:Cytotherapy

    pub_type: 杂志文章,评审

    doi:10.1080/14653240510027172

    authors: Robinson S,Niu T,de Lima M,Ng J,Yang H,McMannis J,Karandish S,Sadeghi T,Fu P,del Angel M,O'Connor S,Champlin R,Shpall E

    更新日期:2005-01-01 00:00:00

  • Ex vivo immunological evaluation of stable mixed chimeric patients after matched related donor allogeneic transplantation in sickle cell disease.

    abstract:BACKGROUND AIMS:Allogeneic hematopoietic stem cell transplantation is curative for sickle cell disease, and the use of matched related donors, non-myeloablative conditioning and sirolimus immunosuppression results in stable mixed chimerism without graft-versus-host disease (GVHD). However, the time to terminate sirolim...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1016/j.jcyt.2019.10.003

    authors: Raines LN,Hsieh MM,Nassehi T,Drysdale CM,Tisdale JF,Uchida N

    更新日期:2019-12-01 00:00:00

  • Immunogenic potential of human bone marrow mesenchymal stromal cells is enhanced by hyperthermia.

    abstract:BACKGROUND AIMS:Bone marrow-derived mesenchymal stromal cells (MSCs) have been reported to suppress T-cell proliferation and used to alleviate the symptoms of graft-versus-host disease (GVHD). MSCs are a mixed cell population and at this time there are no tools to isolate the cells responsible for the T-cell suppressio...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1016/j.jcyt.2018.10.002

    authors: McClain-Caldwell I,Vitale-Cross L,Mayer B,Krepuska M,Boyajian M,Myneni V,Martin D,GENOMICS AND COMPUTATIONAL BIOLOGY CORE.,Marko K,Nemeth K,Mezey E

    更新日期:2018-12-01 00:00:00

  • Summary of the National Institute of Standards and Technology and US Food And Drug Administration cell counting workshop: Sharing practices in cell counting measurements.

    abstract::The emergence of cell-based therapeutics has increased the need for high-quality, robust and validated measurements for cell characterization. Cell count, being one of the most fundamental measures for cell-based therapeutics, now requires increased levels of measurement confidence. The National Institute of Standards...

    journal_title:Cytotherapy

    pub_type: 杂志文章,评审

    doi:10.1016/j.jcyt.2018.03.031

    authors: Lin-Gibson S,Sarkar S,Elliott JT

    更新日期:2018-06-01 00:00:00

  • Exosome-transmitted LINC00461 promotes multiple myeloma cell proliferation and suppresses apoptosis by modulating microRNA/BCL-2 expression.

    abstract:BACKGROUND:Multiple myeloma (MM) is a hematologic cancer caused by the abnormal expansion of plasma cells, but the exact mechanism underlying MM development is not completely known. Recently, multiple long noncoding RNAs (lncRNAs) were implicated in the regulation of MM development. METHODS:Samples from patients with ...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1016/j.jcyt.2018.10.006

    authors: Deng M,Yuan H,Liu S,Hu Z,Xiao H

    更新日期:2019-01-01 00:00:00

  • Adoptive transfer of mesenchymal stromal cells accelerates intestinal epithelium recovery of irradiated mice in an interleukin-6-dependent manner.

    abstract:BACKGROUND AIMS:Apoptosis of radiosensitive cells in the bone marrow and gut is a serious, at times life-threatening, complication arising from radiation exposure. METHODS:We investigated whether adoptive transfer of allogeneic bone marrow-derived mesenchymal stromal cells (MSC) could exert cytoprotective and life-spa...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.3109/14653249.2012.684378

    authors: François M,Birman E,Forner KA,Gaboury L,Galipeau J

    更新日期:2012-11-01 00:00:00

  • Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International So

    abstract:BACKGROUND AIMS:Adipose tissue is a rich and very convenient source of cells for regenerative medicine therapeutic approaches. However, a characterization of the population of adipose-derived stromal and stem cells (ASCs) with the greatest therapeutic potential remains unclear. Under the authority of International Fede...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1016/j.jcyt.2013.02.006

    authors: Bourin P,Bunnell BA,Casteilla L,Dominici M,Katz AJ,March KL,Redl H,Rubin JP,Yoshimura K,Gimble JM

    更新日期:2013-06-01 00:00:00

  • Differential properties of human stromal cells from bone marrow, adipose, liver and cardiac tissues.

    abstract:BACKGROUND AIMS:Mesenchymal stromal cells (MSCs), derived from several tissues including bone marrow and adipose tissue, are being evaluated in clinical trials for a range of diseases. Virtually all tissues of the body contain stromal cells, yet it is unknown whether these sources are similar in phenotype and function....

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1016/j.jcyt.2015.07.009

    authors: Kellner J,Sivajothi S,McNiece I

    更新日期:2015-11-01 00:00:00

  • Managing particulates in cellular therapy.

    abstract::he concept of particulates, while common to many in the pharmaceutical and blood transfusion disciplines, represents a distinct challenge in the field of cellular therapy. With newly discovered products advancing through clinical trials, the focus has shifted to ensuring products are manufactured in a reliable and saf...

    journal_title:Cytotherapy

    pub_type: 杂志文章,评审

    doi:10.3109/14653249.2012.706709

    authors: Clarke D,Harati D,Martin J,Rowley J,Keller J,McCaman M,Carrion M,Karnieli O,Maziarz R,Perry R,Oh S,Stanton J

    更新日期:2012-10-01 00:00:00

  • Similar proteomic profiles of human mesenchymal stromal cells from different donors.

    abstract:BACKGROUND AIMS:Bone marrow (BM) stromal cells, also referred to as mesenchymal stromal cells (MSC), can be expanded ex vivo and are able to differentiate along multiple lineages, including chondrocytes, osteoblasts and adipocytes. MSC are known to secrete a number of cytokines and regulatory molecules implicated in di...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1080/14653240902783268

    authors: Lazzarotto-Silva C,Binato R,Rocher BD,Costa JA,Pizzatti L,Bouzas LF,Abdelhay E

    更新日期:2009-01-01 00:00:00

  • A roundtable on responsible innovation with autologous stem cells in Australia, Japan and Singapore.

    abstract::We report on a roundtable event hosted in Singapore that sought to identify some of the ethical and regulatory challenges in translating autologous cell-based interventions, particularly those claiming to involve stem cells, into safe and effective therapies and to propose some solutions to encourage responsible innov...

    journal_title:Cytotherapy

    pub_type:

    doi:10.1016/j.jcyt.2018.06.004

    authors: Lysaght T,Munsie M,Castricum A,Hui JHP,Okada K,Sato Y,Sawa Y,Stewart C,Tan LK,Tan LHY,Sugii S

    更新日期:2018-09-01 00:00:00

  • Generation of potent dendritic cells with improved migration ability through p-cofilin and sarco/endoplasmic reticulum Ca(2+) transport ATPase 2 regulation.

    abstract:BACKGROUND AIMS:It is important to improve the migratory ability of dendritic cells (DCs) and to increase DC potency for successful DC-based cancer immunotherapy. The intracellular Ca(2+) signaling pathway has an important role on the regulation of DC migration. Our preliminary studies revealed that sarco/endoplasmic r...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1016/j.jcyt.2015.06.002

    authors: Choi NR,Lee HJ,Jung SH,Hong CY,Vo MC,Hoang MD,Kim HJ,Lee JJ

    更新日期:2015-10-01 00:00:00

  • BM-derived cells restore expression of peroxiredoxin V in the airways following acute naphthalene injury in mice.

    abstract:BACKGROUND:Naphthalene-induced respiratory tract toxicity in mice is characterized by specific and rapid loss of the Clara cell population, which is restored only after several days. The sources of restoration of this cell population remain unclear. We investigated whether BM-derived cells participated in the process o...

    journal_title:Cytotherapy

    pub_type: 杂志文章

    doi:10.1080/14653240500361012

    authors: Serikov VB,Popov BV,Kropotov AV,Tomilin NV

    更新日期:2005-01-01 00:00:00