Abstract:
:Pigment epithelium-derived factor (PEDF) is reported to play a protective role against diabetic vascular complications through its anti-oxidative properties. However, since a commercially available kit is not suitable for measurement of serum PEDF in humans, kinetics and regulation of serum PEDF are not known in these devastating disorders. Therefore, we developed a simple, specific and reliable method for measurement of serum PEDF in humans using a competitive enzyme-linked immunosorbent assay (ELISA) system. Assay linearity was shown intact with 50~300-fold dilution of urea-pretreated serum by phosphate-buffered saline. The recovery ratio of added recombinant human PEDF in serum was 94.2 +/- 1.7 %. Inter- and intra-assay coefficient of variations of the ELISA were 4.7 and 7.3 %, respectively. When we measured serum PEDF levels in a general population, PEDF levels were elevated in proportion to the accumulation of the number of the components of the metabolic syndrome. Further, the percent changes in serum levels of PEDF during 1-year observational periods were positively correlated with those of body mass index (BMI) in patients with type 2 diabetes. In addition, PEDF mRNA levels in cultured adipocytes were increased in parallel to the BMI values of subjects from which adipocytes were derived, especially in omental adipocytes. These observations suggest that PEDF is generated from adipose tissues and could be increased as a counter-system against vascular cell damage in humans. PEDF may be one of the useful biomarkers for vascular injury in high-risk patients.
journal_name
Curr Mol Medjournal_title
Current molecular medicineauthors
Fukami K,Yamagishi SI,Okuda Sdoi
10.2174/156652410791065318subject
Has Abstractpub_date
2010-04-01 00:00:00pages
317-20issue
3eissn
1566-5240issn
1875-5666pii
CMM # 37journal_volume
10pub_type
杂志文章abstract::Type 2 diabetes (T2D) is a metabolic disorder characterised by the inability of β-cells to secrete enough insulin to maintain glucose homeostasis. Pancreatic β-cells secrete insulin in a biphasic manner, first and second phase insulin secretion, and loss of first phase insulin secretion is an independent predictor of ...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:In vertebrates, cilium is crucial for Hedgehog signaling transduction. Forkhead box transcriptional factor FoxF1 is reported to be associated with Sonic Hedgehog (Shh) signaling in many cases. However, the role of FoxF1 in cilium remains unknown. Here, we showed an essential role of FoxF1 in the regulation o...
journal_title:Current molecular medicine
pub_type: 信件
doi:10.2174/1566524019666190405115420
更新日期:2019-01-01 00:00:00
abstract::Cancer is one of the most important disorders which is associated with high mortality and high costs of treatment for patients. Despite several efforts, finding, designing and developing, new therapeutic platforms in treatment of cancer patients are still required. Utilization of microorganisms particularly bacteria h...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524020999200817113730
更新日期:2020-08-17 00:00:00
abstract::Pancreatic Adenocarcinoma (PDAC) is one of the most deadly malignant tumors worldwide. A variety of mechanisms are involved in PDAC biological behaviors, of which, the mechanisms of immune escape may be a pivotal hallmark. HLA-G is a tolerant molecule implicated in tumor escape and serves as a prognostic biomarker in ...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524015666150401102218
更新日期:2015-01-01 00:00:00
abstract::Plaque disruption and subsequent thrombus formation play a critical role in the clinical manifestations of atherothrombosis. Vulnerable lesions are characterized by the existence of core rich in lipid, macrophages and tissue factor (TF). Plaque disruption facilitates the interaction between flowing blood with the inne...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652406778018707
更新日期:2006-08-01 00:00:00
abstract::The generation of functional neural progenitor cells (NPCs) independent of donor brain tissue and embryonic tissues is of great therapeutic interest with regard to regenerative medicine and the possible treatment of neurodegenerative disorders. Traditionally, NPCs are derived through the differentiation of embryonic s...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/156652412798889018
更新日期:2012-02-01 00:00:00
abstract::A major benefit of the genomics revolution in biomedical research has been the establishment of transcriptome analysis as an enabling technology in the drug development process. Nowhere in the realm of drug development has the expectation of the impact of transcriptome analysis been greater than in the area of pre-cli...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524053152825
更新日期:2005-02-01 00:00:00
abstract::Several species of kinetoplastid protozoa cause major human infectious diseases. Trypanosoma cruzi is responsible for the fatal Chagas disease in large parts of South America, the various species of Leishmania cause a number of different human diseases with millions of patients world-wide, and the African trypanosome ...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524043360113
更新日期:2004-09-01 00:00:00
abstract::Visfatin is one of the prominent adipokines secreted by adipose tissue. The level of visfatin increases significantly in persons with obesity owing to increased body mass index (BMI). During obesity, the adipocytes, which populate adipose tissue, undergo hypertrophy and hyperplasia and secrete a number of adipocytokin...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524018666180705114131
更新日期:2018-01-01 00:00:00
abstract::Although it has been known for long time that atherosclerosis is associated with lipid deposition, only recently it has been accepted that the plasmatic concentration of cholesterol, especially LDL cholesterol, is a risk factor for atherosclerosis. However, chemically modified LDL, but not native LDL, is able to induc...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524023362177
更新日期:2002-09-01 00:00:00
abstract::Genome-wide association studies (GWAS) have become increasingly widely used to determine regions of the genome which may contain loci influencing the risk of neurological disorders. While linkage studies have identified genes that cause a number of Mendelian disorders, linkage analysis is less well suited for the more...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652409789105534
更新日期:2009-09-01 00:00:00
abstract::The ocular lens is a non-vascular and non-innervated transparent organ that plays an important role in vision processing. This unique organ is derived from the embryonic ectoderm of the brain region through a complicated differentiation process in which apoptosis plays a key role. First, when the committed ectoderm be...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652410793937741
更新日期:2010-12-01 00:00:00
abstract::Endometriosis is one of the most common gynecological inflammatory diseases, occurring in adolescents and women in the reproductive age group and leading to infertility. The precise etiopathogenesis of endometriosis is unknown, but several theories concerning the phenomena involved in its development have been propose...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524015666150921105218
更新日期:2015-01-01 00:00:00
abstract::p53 tumor suppressor plays a pivotal role in maintaining genomic integrity and preventing cancer development. The importance of p53 in tumor suppression is illustrated by the observation that about 50% human tumor cells have a dysfunctional p53 pathway. Although it has been well accepted that the activity of p53 is ma...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652408786733748
更新日期:2008-12-01 00:00:00
abstract::Evidence shows that artificially lowering body and brain temperature can significantly reduce the deleterious effects of brain injury in both newborns and adults. Although the benefits of therapeutic hypothermia have long been known and applied clinically, the underlying molecular mechanisms have yet to be elucidated....
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652412803833517
更新日期:2012-12-01 00:00:00
abstract::Necrotic cell death has long been considered an accidental and uncontrolled mode of cell death. But recently it has become clear that necrosis is a molecularly regulated event that is associated with pathologies such as ischemia-reperfusion (IR) injury, neurodegeneration and pathogen infection. The serine/threonine ki...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652408784221306
更新日期:2008-05-01 00:00:00
abstract::Although GHRH and GHRH-R are recognized as key factors in placental development, little is known about the mechanism(s) of the regulation in trophoblastic cells during placental development. The objective of this study is to determine the potential relationship between the expression levels of GHRH-R and the placental...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524016666160225154040
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Lauric acid (LA) has antimicrobial effects and the potential to replace antibiotics in feeds to prevent postweaning diarrhea and increase overall swine productivity. The effects of lauric acid on the intestinal epithelial cells remain unclear. METHODS AND RESULTS:This study investigates the effects of LA on...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524020666200128155115
更新日期:2020-01-01 00:00:00
abstract::In 1968 Berger and Hinglais published the first description of IgA nephropathy (IgAN). In the ensuing 30 years, extensive clinical, epidemiologic, and immunologic characterizations of primary (idiopathic) glomerulonephritis with IgA as the predominant or co-dominant immunoglobulin deposited in the mesangia of all glom...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524013363852
更新日期:2001-05-01 00:00:00
abstract::Since the discovery almost fifteen years ago that E2F transcription factors are key targets of the retinoblastoma protein (RB), studies of the E2F family have uncovered critical roles in the control of transcription, cell cycle and apoptosis. E2F proteins are encoded by at least eight genes, E2F1 through E2F8. While s...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524010606070739
更新日期:2006-11-01 00:00:00
abstract::Enteroviruses are common human pathogens involved in a wide spectrum of clinical outcomes ranging from mild or non-symptomatic illness to severe diseases with neurological and/or cardiac manifestation. Despite being responsible for significant morbidity and mortality especially in immunocompromised patients and infant...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524011009060550
更新日期:2010-08-01 00:00:00
abstract:BACKGROUND & OBJECTIVE:Interleukin 37 (IL-37) is an important regulator of the anti-inflammatory T-cell response. In this study, we investigated its expression and function in peripheral blood mononuclear cells (PBMCs) of patients with HLA-B27 associated acute anterior uveitis (AAU) and idiopathic AAU. METHODS:15 pati...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524018666180207152941
更新日期:2018-03-09 00:00:00
abstract::Conformational or misfolding diseases are a large class of devastating human disorders associated with protein misfolding and aggregation. Most conformational diseases are caused by a combination of genetic and environmental factors, suggesting that spontaneous events can destabilize the protein involved in the pathol...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652408783565595
更新日期:2008-02-01 00:00:00
abstract::Mood disorders and schizophrenia share a number of common properties, including: genetic susceptibility; differences in brain structure and drug based therapy. Some genetic loci may even confer susceptibility for bipolar mood disorder and schizophrenia, and some atypical antipsychotic drugs are used as mood stabilizer...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524033479672
更新日期:2003-08-01 00:00:00
abstract::Although surgery and radiotherapy are highly efficient in local tumor control, distal metastases and tumor recurrence often limit therapeutic outcome. It is becoming progressively more evident that curative tumor therapy depends on the presence and maintenance of an intact immune system which has the capacity to elici...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652412803306666
更新日期:2012-11-01 00:00:00
abstract::Halitosis (bad breath) is estimated to influence more than half of the world's population with varying degree of intensity. More than 85% of halitosis originates from oral bacterial infections. Foul-smelling breath mainly results from bacterial production of volatile sulfur compounds (VSCs) such as hydrogen sulfide an...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/15665240113139990063
更新日期:2013-09-01 00:00:00
abstract::Strong epidemiological evidence indicates that migraine, especially migraine with aura, is associated with increased risk of ischemic stroke. However, the precise mechanisms of such a relation are currently not fully elucidated and are still a matter of speculation. Migraine may directly cause an ischemic event (i.e, ...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652409787581583
更新日期:2009-03-01 00:00:00
abstract::PTEN (Phosphatase and Tensin homologue deleted on chromosome 10, 10q23.3) is the dominant phosphatase responsible for the dephosphorylation of the 3-position phosphate from the inositol ring of phosphatidylinositol 3,4,5 triphosphate (PIP3), and thereby directly antagonizes the actions mediated by Phosphatidylinositol...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524013666131118100542
更新日期:2014-01-01 00:00:00
abstract::Immune tolerance can be induced by numerous methods. This review article aims to draw lines of similarity and contrast between two unique models of immune tolerance, namely Anterior Chamber Associated Immune Deviation (ACAID) and Nickel-induced oral tolerance. ACAID is an immune tolerance model that leads to the gener...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524015666150114114602
更新日期:2015-01-01 00:00:00
abstract:PURPOSE:Retinal ganglion cells (RGCs) apoptosis is a common characteristic of optic neuropathies. p53-induced protein with a death domain (PIDD) is a well-known regulator of genotoxic stress-induced apoptosis, which is constitutively cleaved into three main fragments: PIDD-N, PIDD-C and PIDD-CC. Thus, we aim to determi...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524019666190918160032
更新日期:2019-01-01 00:00:00