Finding sRNA generative locales from high-throughput sequencing data with NiBLS.

Abstract:

BACKGROUND:Next-generation sequencing technologies allow researchers to obtain millions of sequence reads in a single experiment. One important use of the technology is the sequencing of small non-coding regulatory RNAs and the identification of the genomic locales from which they originate. Currently, there is a paucity of methods for finding small RNA generative locales. RESULTS:We describe and implement an algorithm that can determine small RNA generative locales from high-throughput sequencing data. The algorithm creates a network, or graph, of the small RNAs by creating links between them depending on their proximity on the target genome. For each of the sub-networks in the resulting graph the clustering coefficient, a measure of the interconnectedness of the subnetwork, is used to identify the generative locales. We test the algorithm over a wide range of parameters using RFAM sequences as positive controls and demonstrate that the algorithm has good sensitivity and specificity in a range of Arabidopsis and mouse small RNA sequence sets and that the locales it generates are robust to differences in the choice of parameters. CONCLUSIONS:NiBLS is a fast, reliable and sensitive method for determining small RNA locales in high-throughput sequence data that is generally applicable to all classes of small RNA.

journal_name

BMC Bioinformatics

journal_title

BMC bioinformatics

authors

MacLean D,Moulton V,Studholme DJ

doi

10.1186/1471-2105-11-93

subject

Has Abstract

pub_date

2010-02-18 00:00:00

pages

93

issn

1471-2105

pii

1471-2105-11-93

journal_volume

11

pub_type

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