Evaluation of the CellaVision DM automated microscope in pediatrics.

Abstract:

:The DM is an automated microscope, which performs WBC differential counts and monitors red cell morphology. The user either validates the cell recognition if the DM has correctly identified the WBCs or reclassifies the WBCs in the good category in case of a DM mis-assignment. Morphological anomalies of leukocytes, red blood cells or platelets are analyzed and registered. We studied 521 newborns and infants sorted by age and pathology. The results correlated well with those using conventional microscopy except for samples containing blasts, in which the percentage of malignant cells was underestimated. Newborns had the lowest rates of overall accuracy and postclassification agreement. For red cell analysis, 10% of the selected areas were considered unreadable. However, the DM diagnosed faithfully all studied red cell pathologies. The DM was also very useful in analyzing samples of storage diseases. Timesavings ranging from 1 up to 10 min (for vacuolated lymphocyte screening) were observed when performing analysis with the DM. The DM represents a useful diagnostic and training tool. However, conventional microscopy remains essential, in particular when the image quality is poor, such as in the case of lymphoblasts, and in the screening of platelet clusters.

journal_name

Int J Lab Hematol

authors

Billard M,Lainey E,Armoogum P,Alberti C,Fenneteau O,Da Costa L

doi

10.1111/j.1751-553X.2009.01219.x

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

530-8

issue

5

eissn

1751-5521

issn

1751-553X

pii

CLH1219

journal_volume

32

pub_type

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