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Characterization of the Sesbania rostrata phytochelatin synthase gene: alternative splicing and function of four isoforms.

Abstract：

:Phytochelatins (PCs) play an important role in detoxification of heavy metals in plants. PCs are synthesized from glutathione by phytochelatin synthase (PCS), a dipeptidyltransferase. Sesbania rostrata is a tropical legume plant that can tolerate high concentrations of Cd and Zn. In this study, the S. rostrata PCS gene (SrPCS) and cDNAs were isolated and characterized. Southern blot and sequence analysis revealed that a single copy of the SrPCS gene occurs in the S. rostrata genome, and produces four different SrPCS mRNAs and proteins, SrPCS1-SrPCS4, by alternative splicing of the SrPCS pre-mRNA. The SrPCS1 and SrPCS3 proteins conferred Cd tolerance when expressed in yeast cells, whereas the SrPCS2 and SrPCS4 proteins, which lack the catalytic triad and the N-terminal domains, did not. These results suggested that SrPCS1 and SrPCS3 have potential applications in genetic engineering of plants for enhancing heavy metal tolerance and phytoremediation of contaminated soils.

journal_name

Int J Mol Sci

journal_title

International journal of molecular sciences

authors

Li AM,Yu BY,Chen FH,Gan HY,Yuan JG,Qiu R,Huang JC,Yang ZY,Xu ZF

doi

10.3390/ijms10083269

subject

Has Abstract

pub_date

2009-07-24 00:00:00

pages

3269-82

issue

8

issn

1422-0067

journal_volume

10

pub_type

杂志文章

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