Abstract:
:The ATP-dependent chromatin assembly and remodelling factor (ACF) functions to generate regularly spaced nucleosomes, which are required for heritable gene silencing. The mechanism by which ACF mobilizes nucleosomes remains poorly understood. Here we report a single-molecule FRET study that monitors the remodelling of individual nucleosomes by ACF in real time, revealing previously unknown remodelling intermediates and dynamics. In the presence of ACF and ATP, the nucleosomes exhibit gradual translocation along DNA interrupted by well-defined kinetic pauses that occurred after approximately seven or three to four base pairs of translocation. The binding of ACF, translocation of DNA and exiting of translocation pauses are all ATP-dependent, revealing three distinct functional roles of ATP during remodelling. At equilibrium, a continuously bound ACF complex can move the nucleosome back-and-forth many times before dissociation, indicating that ACF is a highly processive and bidirectional nucleosome translocase.
journal_name
Naturejournal_title
Natureauthors
Blosser TR,Yang JG,Stone MD,Narlikar GJ,Zhuang Xdoi
10.1038/nature08627subject
Has Abstractpub_date
2009-12-24 00:00:00pages
1022-7issue
7276eissn
0028-0836issn
1476-4687pii
nature08627journal_volume
462pub_type
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