Radioimmunoassay of herpes-simplex and measles virus antibodies in serum and cerebrospinal fluid of patients without infectious or demyelinating diseases of the central nervous system.

Abstract:

:A solid-phase radioimmunoassay was used to detect IgG antibodies against herpes-simplex virus antigens (capsid, envelope and excreted) and against measles virus antigen in serum and cerebrospinal fluid (CSF) specimens of 61 patients with no evidence of infectious or demyelinating disease of the central nervous system. Quantitative determinations of IgG and albumin in serum and CSF were also performed. Of the 61 serum and 61 CSF samples tested, 57 and 56 respectively contained antibodies against subunit antigens of herpes simplex virus. Antibody against measles virus was found in 59 serum and 47 CSF specimens. A positive correlation (P less than 0-001) was found between each of the four serum to CSF antibody ratios and the serum to CSF total IgG ratios. This indicated that the distribution of antiviral IgG antibodies in serum and CSF normally follows the distribution of total IgG. The ratios between viral antibody in serum and CSF were also correlated with albumin ratios (P less than 0-05). An inverse relation (P less than 0-001) was found between the age of the patients and their serum to CSF albumin ratios, but not their IgG ratios, suggesting that the albumin ratio is a useful indicator of a blood brain barrier lesion and that the IgG ratio should be used in evaluating disturbed antibody ratios.

journal_name

J Med Microbiol

authors

Kalimo KO,Marttila RJ,Ziola BR,Matikainen MT,Panelius M

doi

10.1099/00222615-10-4-431

subject

Has Abstract

pub_date

1977-11-01 00:00:00

pages

431-8

issue

4

eissn

0022-2615

issn

1473-5644

journal_volume

10

pub_type

杂志文章
  • In-vitro characterisation of the phagocytosis and fate of anthrax spores in macrophages and the effects of anti-PA antibody.

    abstract::Antibodies (Abs) to the protective antigen (PA) component of the anthrax toxins have anti-spore as well as anti-toxin activities. Anti-PA antisera and purified anti-PA Abs enhance the phagocytosis by murine-derived macrophages (MQs) of spores of the Ames and Sterne strains and retard the germination of extracellular s...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-51-10-821

    authors: Welkos S,Friedlander A,Weeks S,Little S,Mendelson I

    更新日期:2002-10-01 00:00:00

  • Protection against experimental salmonellosis in mice and sheep by immunisation with aromatic-dependent Salmonella typhimurium.

    abstract::Mice immunised by the oral or intraperitoneal route with a live aromatic-dependent strain of Salmonella typhimurium exhibited significantly less protection against oral challenge with 50 LD50 of an ovine isolate of S. typhimurium (12313) than when a bovine isolate with the same O antigens and phage-type as strain 1231...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-24-1-11

    authors: Mukkur TK,McDowell GH,Stocker BA,Lascelles AK

    更新日期:1987-08-01 00:00:00

  • Liver abscess caused by Brevundimonas vesicularis in an immunocompetent patient.

    abstract::Invasive infections caused by Brevundimonas vesicularis are very rare in humans. We experienced an unusual case of liver abscess due to B. vesicularis in an immunocompetent young male. The patient was successfully treated by liver abscess drainage and with antimicrobial therapy of ceftriaxone followed by ampicillin/su...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.045120-0

    authors: Yoo SH,Kim MJ,Roh KH,Kim SH,Park DW,Sohn JW,Yoon YK

    更新日期:2012-10-01 00:00:00

  • Intracellular, biofilm-inhibitory and membrane-damaging activities of nimbolide isolated from Azadirachta indica A. Juss (Meliaceae) against meticillin-resistant Staphylococcus aureus.

    abstract::Staphylococcus aureus is a leading aetiologic agent of nosocomial- and community-acquired infectious diseases worldwide. The public health concern regarding staphylococcal infections is inflated by the increasing occurrence of multidrug-resistant strains, e.g. multidrug- and meticillin-resistant S.aureus (MDR MRSA). T...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000343

    authors: Sarkar P,Acharyya S,Banerjee A,Patra A,Thankamani K,Koley H,Bag PK

    更新日期:2016-10-01 00:00:00

  • Identification of urinary pathogens by the RAPIDEC ur system.

    abstract::A commercial method (RAPIDEC ur; bioMérieux UK Ltd, Basingstoke) and an in-house scheme based on conventional tests were compared for the identification of 179 commonly encountered gram-negative urinary pathogens. The methods identified correctly to genus level 97% and 84% of organisms, respectively. RAPIDEC ur was su...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-43-1-72

    authors: Batchelor BI

    更新日期:1995-07-01 00:00:00

  • An improved selective medium for the isolation of Escherichia coli O157.

    abstract::Sorbitol-MacConkey medium has become widely used for the isolation of verotoxigenic (VT+) Escherichia coli O157. However, many organisms other than VT+ E. coli O157, especially other serogroups of E. coli and Proteus spp., may not ferment sorbitol, and thus may be confused initially with VT+ E. coli O157. Rhamnose is ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-35-2-107

    authors: Chapman PA,Siddons CA,Zadik PM,Jewes L

    更新日期:1991-08-01 00:00:00

  • Identification of an immunogenic 18-kDa protein of Helicobacter pylori by alkaline phosphatase gene fusions.

    abstract::The use of alkaline phosphatase fusion methodology to identify Helicobacter pylori exported proteins enabled the identification of an open reading frame (ORF) encoding a highly immunogenic, previously uncharacterised exported protein. The predicted aminoacid sequence displays a typical N-terminal signal peptide and co...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-49-7-643

    authors: Oliaro J,Johnson RD,Chen W,Chadwick VS,Murray A

    更新日期:2000-07-01 00:00:00

  • Same-day antimicrobial susceptibility test using acoustic-enhanced flow cytometry visualized with supervised machine learning.

    abstract::Purpose. Antimicrobial susceptibility is slow to determine, taking several days to fully impact treatment. This proof-of-concept study assessed the feasibility of using machine-learning techniques for analysis of data produced by the flow cytometer-assisted antimicrobial susceptibility test (FAST) method we developed....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001092

    authors: Inglis TJJ,Paton TF,Kopczyk MK,Mulroney KT,Carson CF

    更新日期:2020-05-01 00:00:00

  • Resistance to carbapenems in sequence type 11 Klebsiella pneumoniae is related to DHA-1 and loss of OmpK35 and/or OmpK36.

    abstract::This study investigated the molecular mechanisms of carbapenem resistance in clinical isolates of Klebsiella pneumoniae from Korea and the clinical outcomes of resistant K. pneumoniae infection. Sixteen K. pneumoniae isolates showing resistance to carbapenems collected from a tertiary-care hospital were examined for t...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.037036-0

    authors: Shin SY,Bae IK,Kim J,Jeong SH,Yong D,Kim JM,Lee K

    更新日期:2012-02-01 00:00:00

  • Experimental rabbit model of meningitis produced by Haemophilus influenzae serotype c.

    abstract::The virulence of Haemophilus influenzae type c when inoculated intracisternally (i.c.) into rabbits was evaluated. Rabbits are relatively resistant to infection with H. influenzae type b, such that inocula of the order of 10(6-9) cfu are required to produce meningitis in this model. In contrast, fatal meningitis was p...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-36-5-312

    authors: Sulc P,Hengstler B,Krinke G,O'Reilly T,Zak O

    更新日期:1992-05-01 00:00:00

  • Virulent Toxoplasma gondii strain RH promotes T-cell-independent overproduction of proinflammatory cytokines IL12 and gamma-interferon.

    abstract::The aim of this study was the analysis of the cytokine response in BALB/c mice infected with the highly virulent RH or the weakly virulent Beverley strains of Toxoplasma gondii. Analysis of cytokine messages showed increased expression of IL12, IFN-gamma and TNF-alpha, but not IL4 mRNAs in spleen cells after infection...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.04860-0

    authors: Nguyen TD,Bigaignon G,Markine-Goriaynoff D,Heremans H,Nguyen TN,Warnier G,Delmee M,Warny M,Wolf SF,Uyttenhove C,Van Snick J,Coutelier JP

    更新日期:2003-10-01 00:00:00

  • Phagocytosis and ingestion of influenza virus by human polymorphonuclear leucocytes in vitro: electronmicroscopy studies.

    abstract::Interaction of human polymorphonuclear leucocytes (PMNL) and influenza virus (IFV) was studied in vitro. At 0 degree C, the viral particles were bound extensively to the surface of the PMNL membrane with a ratio of about 1000 virus particles to a single PMNL. The binding was sensitive to neuraminidase, suggesting atta...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-28-3-191

    authors: Yamamoto K,Suzuki K,Suzuki K,Mizuno S

    更新日期:1989-03-01 00:00:00

  • In-vitro anti-chlamydial activities of free and liposomal tetracycline and doxycycline.

    abstract::The purpose of this study was to evaluate the anti-chlamydial activities in vitro of liposome-encapsulated doxycycline (Dox) and tetracycline (Tet) in comparison with free Dox and Tet. Dox and Tet encapsulated in cationic (CAL), anionic (ANL) and neutral (NTL) liposomes by sonication, were quantified by high-performan...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-48-7-689

    authors: Sangare L,Morisset R,Ravaoarinoro M

    更新日期:1999-07-01 00:00:00

  • Vaginal carriage and neonatal acquisition of Clostridium difficile.

    abstract::The relationship between vaginal carriage and subsequent neonatal acquisition of Clostridium difficile was investigated. Vaginal carriage of C. difficile was detected in 11% of women attending the Department of Genital Medicine Clinic. C. difficile was isolated from the vagina in 18% of 50 mothers before delivery, and...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-18-1-47

    authors: Tabaqchali S,O'Farrell S,Nash JQ,Wilks M

    更新日期:1984-08-01 00:00:00

  • Identification of oral bacteria associated with crevicular epithelial cells from chronic periodontitis lesions.

    abstract::Bacterial invasion of host epithelial cells plays an important role in the pathogenesis of periodontal diseases; however, the interactions between subgingival species and the gingival crevice cells are not fully understood. This study determined the prevalence of a group of oral bacterial species on or in epithelial c...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46417-0

    authors: Colombo AV,Silva CM,Haffajee A,Colombo APV

    更新日期:2006-05-01 00:00:00

  • Laboratory-based surveillance of human verocytotoxigenic Escherichia coli infection in the Republic of Ireland, 2002-2004.

    abstract::The aim of this study was to examine the frequency and distribution of human verocytotoxigenic Escherichia coli (VTEC) O157 and non-O157 in the Republic of Ireland, and also to examine the presence of virulence genes in these isolates. This genetic information combined with phenotypic tests was used to produce a compl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46147-0

    authors: Carroll AM,Gibson A,McNamara EB

    更新日期:2005-12-01 00:00:00

  • The host immune response to Clostridium difficile.

    abstract::Clostridium difficile is the most common cause of nosocomial bacterial diarrhoea in the Western world. Diarrhoea and colitis are caused by the actions of toxins A and B released by pathogenic strains of C. difficile. Adaptive immune responses to these toxins influence the outcomes of C. difficile infection (CDI). Symp...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/jmm.0.030015-0

    authors: Kelly CP,Kyne L

    更新日期:2011-08-01 00:00:00

  • Characterisation of Plesiomonas shigelloides strains that share type-specific antigen with Shigella flexneri 6 and common group 1 antigen with Shigella flexneri spp. and Shigella dysenteriae 1.

    abstract::Three strains of Plesiomonas shigelloides isolated from patients with diarrhoea were agglutinated with Shigella flexneri 6 antiserum in slide and tube tests. All the strains were also agglutinated with a monoclonal antibody to the common group 1 antigen shared between S. flexneri serotypes and S. dysenteriae type 1. F...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-39-3-211

    authors: Albert MJ,Ansaruzzaman M,Qadri F,Hossain A,Kibriya AK,Haider K,Nahar S,Faruque SM,Alam AN

    更新日期:1993-09-01 00:00:00

  • Increase in SCCmec type IV strains affects trends in antibiograms of meticillin-resistant Staphylococcus aureus at a tertiary-care hospital.

    abstract::The prevalence of community-acquired meticillin-resistant Staphylococcus aureus (CA-MRSA) strains has become a serious problem worldwide. The aim of this study was to investigate the annual transitions of MRSA strains with the CA-MRSA feature, which were identified as SCCmec type IV or V, in a hospital setting in Japa...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000080

    authors: Ito A,Nakaminami H,Fujii T,Utsumi K,Noguchi N

    更新日期:2015-07-01 00:00:00

  • The bactericidal activity of DR-3355, an optically active isomer of ofloxacin.

    abstract::The bactericidal activity of compound DR-3355, an optically active isomer of ofloxacin, was measured against Escherichia coli, Staphylococcus aureus and S. epidermidis, in nutrient broth and in phosphate-buffered saline. DR-3355 was found to be approximately twice as active as ofloxacin in terms of the concentration a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-30-3-227

    authors: Lewin CS,Amyes SG

    更新日期:1989-11-01 00:00:00

  • Prevalence of trimethoprim resistance genes in Escherichia coli isolates of human and animal origin in Lithuania.

    abstract::A total of 456 non-repetitive Escherichia coli isolates from human clinical specimens (urinary, n=134; cervix, vagina and prostate, n=52; blood, pus and wounds, n=45), healthy animals (cattle, n=45; poultry, n=20) and diseased animals (cattle, n=53; swine, n=64; poultry, n=43) obtained in Lithuania during the period 2...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.015008-0

    authors: Šeputienė V,Povilonis J,Ružauskas M,Pavilonis A,Sužiedėlienė E

    更新日期:2010-03-01 00:00:00

  • Analysis of methods commonly used for glycopeptide and oxazolidinone susceptibility testing in Enterococcus faecium isolates.

    abstract::The susceptibility to teicoplanin, vancomycin and linezolid of 30 clinical isolates of Enterococcus faecium was tested by Vitek 2, Phoenix, Etest, broth microdilution and disc diffusion tests. The vanA and vanB resistance genes and the 23S rRNA gene G2576T mutation were detected by PCR and PCR-RFLP, respectively. Resi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.016444-0

    authors: Raponi G,Ghezzi MC,Gherardi G,Lorino G,Dicuonzo G

    更新日期:2010-06-01 00:00:00

  • Characterisation of Eubacterium-like strains isolated from oral infections.

    abstract::The genus Eubacterium currently includes a heterogeneous group of gram-positive, non-spore-forming anaerobic bacilli, many of which are slow growing, fastidious and generally unreactive in biochemical tests. As a consequence, cultivation and identification of isolates are difficult and the taxonomy of the group remain...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-11-947

    authors: Downes J,Munson MA,Spratt DA,Kononen E,Tarkka E,Jousimies-Somer H,Wade WG

    更新日期:2001-11-01 00:00:00

  • Characterization of the genetic background of Vibrio cholerae O1 biotype El Tor serotype Inaba strains isolated in Trivandrum, southern India.

    abstract::Isolates of Vibrio cholerae O1 biotype El Tor serotype Inaba associated with an outbreak of cholera in Trivandrum, southern India, were characterized. PCR testing revealed that all five isolates examined carried the TCP pathogenicity island, the CTX genetic element and the RTX toxin, and produced cholera toxin (CT). R...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46868-0

    authors: Mohapatra SS,Ramachandran D,Mantri CK,Singh DV

    更新日期:2007-02-01 00:00:00

  • Importance of Providencia species as a major cause of travellers' diarrhoea.

    abstract::In this study the importance of Providencia species as a cause of travellers' diarrhoea was examined using a selective medium developed by the authors. Providencia species could easily be distinguished from other enteric pathogens by the colour of the colonies obtained. Nine strains of Providencia alcalifaciens, nine ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45846-0

    authors: Yoh M,Matsuyama J,Ohnishi M,Takagi K,Miyagi H,Mori K,Park KS,Ono T,Honda T

    更新日期:2005-11-01 00:00:00

  • Isolation and characterization of Siphoviridae phage infecting extensively drug-resistant Acinetobacter baumannii and evaluation of therapeutic efficacy in vitro and in vivo.

    abstract:PURPOSE:Extensively drug-resistant (XDR) strains of Acinetobacter baumannii are being reported worldwide, and they are associated with high morbidity and mortality rates. These strains are considered to be the highest priority for the development of new antibacterial agents. Therefore, we aimed to develop an effective ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001002

    authors: Wintachai P,Naknaen A,Pomwised R,Voravuthikunchai SP,Smith DR

    更新日期:2019-07-01 00:00:00

  • Genome sequence analysis of emm89 Streptococcus pyogenes strains causing infections in Scotland, 2010-2016.

    abstract:PURPOSE:Strains of type emm89 Streptococcus pyogenes have recently increased in frequency as a cause of human infections in several countries in Europe and North America. This increase has been molecular epidemiologically linked with the emergence of a new genetically distinct clone, designated clade 3. We sought to ex...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000622

    authors: Beres SB,Olsen RJ,Ojeda Saavedra M,Ure R,Reynolds A,Lindsay DSJ,Smith AJ,Musser JM

    更新日期:2017-12-01 00:00:00

  • Mechanisms of chloramphenicol resistance in Haemophilus influenzae in the United Kingdom.

    abstract::Of 2458 isolates of Haemophilus influenzae examined in a recent British survey, 42 were resistant to chloramphenicol. Two resistant isolates were of type b and 40 were non-capsulate. Spectrophotometric assay showed that all the resistant isolates produced chloramphenicol acetyltransferase (CAT). CAT activity did not i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-27-2-89

    authors: Powell M,Livermore DM

    更新日期:1988-10-01 00:00:00

  • An erythrocyte-sensitising antigen from Vibrio cholerae.

    abstract::Erythrocyte-sensitising antigens (AE) were prepared from Vibrio cholerae serotypes, from EL-Tor vibrio, Escherichia coli and Salmonella enteritidis by digesting the organisms with NaOH followed by precipitation with alcohol. When AE was used in indirect haemagglutination (IHA) tests, the results in a number of cases w...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-8-1-11

    authors: Garabedian GA,Barsumian EL,Malakian A

    更新日期:1975-02-01 00:00:00

  • Capnocytophaga sputigena primary iliopsoas abscess.

    abstract::Iliopsoas abscess is usually secondary to the spread of infection from a contiguous focus. Primary disease is uncommon, except in children where Staphylococcus aureus is the main pathogen. We report a 60-year-old woman who developed a primary iliopsoas abscess as a result of haematogenous spread of Capnocytophaga sput...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.022905-0

    authors: Chan JFW,Wong SSY,Leung SSM,Li IWS,To KKW,Cheng VCC,Yuen KY

    更新日期:2010-11-01 00:00:00