Abstract：
:The blood-brain barrier (BBB) maintains the homeostasis between the central nervous system and the blood circulation. One of the main efflux transporter proteins at the BBB is P-glycoprotein (P-gP) also known as ABCB1 or MDR1. Due to the important role of P-gP for the transport barrier function of the BBB, the presence and functionality of P-gP was investigated in porcine cell line PBMEC/C1-2. Presence of P-gP was confirmed on the protein level by western blotting and immunofluorescence microscopy as well as on the mRNA level by qPCR. Functional assessment was accomplished by an established 96-well uptake assay using Rhodamine 123 and Doxorubicin as P-gP substrates and Verapamil as moderate P-gP inhibitor. In this regard, fluorescence microscopy confirmed a significant higher uptake of Rhodamine 123 into PBMEC/C1-2 cells when preincubated with Verapamil. Finally, knock-down of P-gP by antisense oligonucleotides revealed an increase of Rhodamine 123 uptake indicating decreased P-gP functionality. In summary, the presence and functionality of P-gP in the immortalised cell line PBMEC/C1-2 was proven with several techniques and assays. Thus, this cell line could be used for P-gP studies in the context of BBB relevant issues.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Neuhaus W,Stessl M,Strizsik E,Bennani-Baiti B,Wirth M,Toegel S,Modha M,Winkler J,Gabor F,Viernstein H,Noe CRdoi
10.1016/j.neulet.2009.11.079subject
Has Abstractpub_date
2010-01-22 00:00:00pages
224-8issue
2eissn
0304-3940issn
1872-7972pii
S0304-3940(09)01570-5journal_volume
469pub_type
杂志文章abstract：:Bis(7)-tacrine [bis(7)-tetrahydroaminacrine] acts as an AChE inhibitor and also exerts modulatory effects on many ligand-gated ion channels and voltage-gated Ca(2+) and K(+) channels. It has been reported previously that tacrine and some other AChE inhibitors suppressed I(K(A)) in central and peripheral neurons. The p...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2006.10.047
更新日期：2007-01-29 00:00:00
abstract：:A growing body of evidence suggests that Alzheimer's disease (AD) is associated with local inflammation processes, including the activation of inflammatory cytokines. We performed a case-control association study between sporadic AD patients and the exon 5 position +3953 polymorphism in the potent pro-inflammatory cyt...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.03.063
更新日期：2004-06-10 00:00:00
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journal_title：Neuroscience letters
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doi：10.1016/j.neulet.2007.09.043
更新日期：2007-11-27 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
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journal_title：Neuroscience letters
pub_type： 杂志文章
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(92)90401-r
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journal_title：Neuroscience letters
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journal_title：Neuroscience letters
pub_type： 杂志文章
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journal_title：Neuroscience letters
pub_type： 杂志文章
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更新日期：1997-01-24 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章,随机对照试验
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journal_title：Neuroscience letters
pub_type： 临床试验,杂志文章
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journal_title：Neuroscience letters
pub_type： 杂志文章
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2010.07.082
更新日期：2010-10-11 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(88)90641-6
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journal_title：Neuroscience letters
pub_type： 杂志文章
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journal_title：Neuroscience letters
pub_type： 杂志文章
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journal_title：Neuroscience letters
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更新日期：2010-03-08 00:00:00
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journal_title：Neuroscience letters
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journal_title：Neuroscience letters
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更新日期：2014-02-21 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
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更新日期：1994-02-14 00:00:00
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journal_title：Neuroscience letters
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更新日期：1986-09-25 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.07.049
更新日期：2004-09-30 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(90)90444-e
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journal_title：Neuroscience letters
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