The application of artificial intelligence to microarray data: identification of a novel gene signature to identify bladder cancer progression.

Abstract:

BACKGROUND:New methods for identifying bladder cancer (BCa) progression are required. Gene expression microarrays can reveal insights into disease biology and identify novel biomarkers. However, these experiments produce large datasets that are difficult to interpret. OBJECTIVE:To develop a novel method of microarray analysis combining two forms of artificial intelligence (AI): neurofuzzy modelling (NFM) and artificial neural networks (ANN) and validate it in a BCa cohort. DESIGN, SETTING, AND PARTICIPANTS:We used AI and statistical analyses to identify progression-related genes in a microarray dataset (n=66 tumours, n=2800 genes). The AI-selected genes were then investigated in a second cohort (n=262 tumours) using immunohistochemistry. MEASUREMENTS:We compared the accuracy of AI and statistical approaches to identify tumour progression. RESULTS AND LIMITATIONS:AI identified 11 progression-associated genes (odds ratio [OR]: 0.70; 95% confidence interval [CI], 0.56-0.87; p=0.0004), and these were more discriminate than genes chosen using statistical analyses (OR: 1.24; 95% CI, 0.96-1.60; p=0.09). The expression of six AI-selected genes (LIG3, FAS, KRT18, ICAM1, DSG2, and BRCA2) was determined using commercial antibodies and successfully identified tumour progression (concordance index: 0.66; log-rank test: p=0.01). AI-selected genes were more discriminate than pathologic criteria at determining progression (Cox multivariate analysis: p=0.01). Limitations include the use of statistical correlation to identify 200 genes for AI analysis and that we did not compare regression identified genes with immunohistochemistry. CONCLUSIONS:AI and statistical analyses use different techniques of inference to determine gene-phenotype associations and identify distinct prognostic gene signatures that are equally valid. We have identified a prognostic gene signature whose members reflect a variety of carcinogenic pathways that could identify progression in non-muscle-invasive BCa.

journal_name

Eur Urol

journal_title

European urology

authors

Catto JW,Abbod MF,Wild PJ,Linkens DA,Pilarsky C,Rehman I,Rosario DJ,Denzinger S,Burger M,Stoehr R,Knuechel R,Hartmann A,Hamdy FC

doi

10.1016/j.eururo.2009.10.029

subject

Has Abstract

pub_date

2010-03-01 00:00:00

pages

398-406

issue

3

eissn

0302-2838

issn

1873-7560

pii

S0302-2838(09)01135-X

journal_volume

57

pub_type

杂志文章
  • Nephroblastoma associated with mesangioproliferative glomerulonephritis.

    abstract::The case of a girl showing left-sided Wilms tumor together with a nephrotic syndrome is presented. Recovery after tumor nephrectomy was uneventful. After cessation of cytostatic chemotherapy chronic renal failure developed. Open wedge biopsy of the right kidney revealed endstage glomerular disease. Renal histology in ...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1159/000473495

    authors: Ringert RH,Pistor K

    更新日期:1982-01-01 00:00:00

  • Near-infrared fluorescence imaging: emerging applications in robotic upper urinary tract surgery.

    abstract:BACKGROUND:Near-infrared fluorescence (NIRF) imaging is a technology with emerging applications in urologic surgery. OBJECTIVE:To describe surgical techniques and provide clinical outcomes for robotic partial nephrectomy (RPN) with selective clamping and robotic upper urinary tract reconstruction featuring novel appli...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1016/j.eururo.2013.09.023

    authors: Bjurlin MA,Gan M,McClintock TR,Volpe A,Borofsky MS,Mottrie A,Stifelman MD

    更新日期:2014-04-01 00:00:00

  • Effects of 3 months' neoadjuvant hormonal treatment with a GnRH analogue (triptorelin) prior to radical retropubic prostatectomy on prostate-specific antigen and tumour volume in prostate cancer.

    abstract::In this retrospective study the relationship between serum prostate-specific antigen (PSA) and tumour volume (assessed by the planimetric method on whole mount section) was analysed in 56 patients subjected to radical prostatectomy, of whom 28 received 3 months of neoadjuvant GnRH analogue (triptorelin) treatment. Ser...

    journal_title:European urology

    pub_type: 临床试验,杂志文章

    doi:10.1159/000475337

    authors: Aus G,Brändstedt S,Häggman M,Pedersen K,de la Torre M,Hugosson J

    更新日期:1994-01-01 00:00:00

  • Prognostic Factors and Treatment Results After Bleomycin, Etoposide, and Cisplatin in Germ Cell Cancer: A Population-based Study.

    abstract:BACKGROUND:First-line treatment for patients with disseminated germ cell cancer (GCC) is bleomycin, etoposide, and cisplatin (BEP). A prognostic classification of patients receiving chemotherapy was published by the International Germ Cell Cancer Collaborative Group (IGCCCG) in 1997, but only a small proportion of the ...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1016/j.eururo.2016.09.015

    authors: Kier MG,Lauritsen J,Mortensen MS,Bandak M,Andersen KK,Hansen MK,Agerbaek M,Holm NV,Dalton SO,Johansen C,Daugaard G

    更新日期:2017-02-01 00:00:00

  • Ureteral reimplantation for management of ureteral strictures: a retrospective comparison of laparoscopic and open techniques.

    abstract:OBJECTIVES:To compare the results of laparoscopic ureteral reimplantation with a previous series of open surgery. MATERIALS AND METHODS:We compared ten patients who underwent laparoscopic vesicopsoas-hitch with (n=4) or without Boari-flap (n=6) technique for ureteral obstructions with ten patients treated by open uret...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1016/j.eururo.2006.08.004

    authors: Rassweiler JJ,Gözen AS,Erdogru T,Sugiono M,Teber D

    更新日期:2007-02-01 00:00:00

  • Toxicity and results of MVAC (methotrexate, vinblastine, adriamycin and cisplatin) chemotherapy in advanced urothelial carcinoma.

    abstract:OBJECTIVE:The experience with MVAC (methotrexate, vinblastine, Adriamycin and cisplatin) chemotherapy in advanced urothelial cancer is reviewed with emphasis on toxicity and efficacy. METHODS:We report on 28 patients with advanced, progressive transitional cell carcinoma (TCC) of the bladder (27) or ureter (1), treate...

    journal_title:European urology

    pub_type: 临床试验,杂志文章

    doi:10.1159/000474499

    authors: Witjes JA,Wullink M,Oosterhof GO,de Mulder P

    更新日期:1997-01-01 00:00:00

  • Opiate antagonists in erectile dysfunction: a possible new treatment option? Results of a pilot study with naltrexone.

    abstract::Opioids have an inhibitory effect on sexual functions in both animals and humans. Twenty patients with idiopathic, nonvascular, nonneurogenic erectile dysfunction were treated with the opiate receptor antagonist naltrexone in a randomized, placebo-controlled, double-blind study for 8 weeks. Libido and frequency of sex...

    journal_title:European urology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1159/000475059

    authors: van Ahlen H,Piechota HJ,Kias HJ,Brennemann W,Klingmüller D

    更新日期:1995-01-01 00:00:00

  • Survival with Newly Diagnosed Metastatic Prostate Cancer in the "Docetaxel Era": Data from 917 Patients in the Control Arm of the STAMPEDE Trial (MRC PR08, CRUK/06/019).

    abstract:BACKGROUND:Prostate cancer (PCa) is the second most common disease among men worldwide. It is important to know survival outcomes and prognostic factors for this disease. Recruitment for the largest therapeutic randomised controlled trial in PCa--the Systemic Therapy in Advancing or Metastatic Prostate Cancer: Evaluati...

    journal_title:European urology

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.eururo.2014.09.032

    authors: James ND,Spears MR,Clarke NW,Dearnaley DP,De Bono JS,Gale J,Hetherington J,Hoskin PJ,Jones RJ,Laing R,Lester JF,McLaren D,Parker CC,Parmar MKB,Ritchie AWS,Russell JM,Strebel RT,Thalmann GN,Mason MD,Sydes MR

    更新日期:2015-06-01 00:00:00

  • Activation of the thromboxane A2 pathway in human prostate cancer correlates with tumor Gleason score and pathologic stage.

    abstract:OBJECTIVE:We investigated the potential involvement of the thromboxane A(2) (TXA(2)) pathway in human prostate cancer (PCa). METHODS:Expression of cyclooxygenase-2 (COX-2), TXA(2) synthase (TXS), and TXA(2) receptors (TPRs), the main actors of the TXA(2) pathway, was analyzed on serial tissue sections from 46 human PC...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1016/j.eururo.2006.01.036

    authors: Dassesse T,de Leval X,de Leval L,Pirotte B,Castronovo V,Waltregny D

    更新日期:2006-11-01 00:00:00

  • The 111 Study: A Single-arm, Phase 3 Trial Evaluating One Cycle of Bleomycin, Etoposide, and Cisplatin as Adjuvant Chemotherapy in High-risk, Stage 1 Nonseminomatous or Combined Germ Cell Tumours of the Testis.

    abstract:BACKGROUND:Standard management in the UK for high-risk stage 1 nonseminoma germ cell tumours of the testis (NSGCTT) is two cycles of adjuvant bleomycin, etoposide (360 mg/m2), and cisplatin (BE360P) chemotherapy, or surveillance. OBJECTIVE:To test whether one cycle of BE500P achieves similar recurrence rates to two cy...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1016/j.eururo.2019.11.022

    authors: Cullen M,Huddart R,Joffe J,Gardiner D,Maynard L,Hutton P,Mazhar D,Shamash J,Wheater M,White J,Goubar A,Porta N,Witts S,Lewis R,Hall E,111 Trial Management Group.

    更新日期:2020-03-01 00:00:00

  • Urothelial histopathology surrounding bladder carcinomas.

    abstract::We studied the histological condition of the urothelium surrounding bladder carcinomas in 65 patients who underwent partial resection of the bladder. These examinations showed that urothelial abnormalities were always present, i.e. a direct transition from tumour to normal urothelium was never observed. The histologic...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1159/000472763

    authors: Brünger R,Schubert GE

    更新日期:1987-01-01 00:00:00

  • Diagnostic and therapeutic problems in 10 cases of pheochromocytomae.

    abstract::Ten cases of pheochromocytomae, of which eight were benign and two malignant have been considered. All patients presented with hypertension, either paroxysmal (7 cases) or permanent (3 cases). It is essential to emphasize that the initial symptomatology of these patients on their admission to the hospital was often at...

    journal_title:European urology

    pub_type: 杂志文章

    doi:

    authors: Dimopoulos C,Scouteris M,Hatziconstantinou B,Polyzos L,Ballas P,Daicos G

    更新日期:1975-01-01 00:00:00

  • Clinical progress with a new antiandrogen, Casodex (bicalutamide).

    abstract::Casodex (bicalutamide, Zeneca Limited) is a nonsteroidal competitive inhibitor of androgens at the androgen receptor. The drug was developed to fulfil a number of needs for the treatment of prostate cancer. The specific aim was to demonstrate pharmacological activity, which would translate into clinical efficacy, good...

    journal_title:European urology

    pub_type: 杂志文章,评审

    doi:10.1159/000473847

    authors: Blackledge GR

    更新日期:1996-01-01 00:00:00

  • Clinical application of the Bardex IC Foley catheter.

    abstract:OBJECTIVES:We performed two randomized prospective studies with the silver-coated Bardex IC catheter in order to evaluate the incidence of bacteriuria during short- and medium-term catheterization after urological procedures. METHODS:During catheterization only consecutive suprapubic urine samples were taken and cultu...

    journal_title:European urology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1159/000068005

    authors: Verleyen P,De Ridder D,Van Poppel H,Baert L

    更新日期:1999-09-01 00:00:00

  • Cystinuria in children.

    abstract::7 children, 20 months to 11 years old, with cystinuria and renal calculi were studied. Surgical treatment and alpha-mercaptopropionylglycine (MPG) gave satisfactory results in 5 children. The causes of the recurrences in the other 2 children are discussed. MPG therapy is effective but can cause a nephrotic syndrome at...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1159/000473204

    authors: Pavanello L,Rizzoni G,Dussini N,Zacchello G,Passerini G,Tasca A,Pagano F

    更新日期:1981-01-01 00:00:00

  • Sigmoid kidney associated with renal adenocarcinoma.

    abstract::A rare case of adenocarcinoma in a sigmoid kidney is reported. The literature is reviewed and the operative technique described. Preoperative arteriogram and cavogram are an essential part of the evaluation and provide aid in the surgical approach. ...

    journal_title:European urology

    pub_type: 杂志文章

    doi:

    authors: Vandendris M,Schulman CC,Struyven J,Grégoir W

    更新日期:1975-01-01 00:00:00

  • Squamous cell carcinoma of bladder diverticulum with initial symptoms produced by metastasis to maxillary sinus.

    abstract::A rare case of squamous cell carcinoma in the bladder diverticula of a 74-year-old man with initial clinical symptoms caused by metastasis to the maxillary sinus is reported. The histopathological examination at autopsy confirmed the metastatic squamous cell carcinoma in the maxillary sinus from the bladder diverticul...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1159/000473453

    authors: Nanbu A,Tsukamoto T,Kumamoto Y,Aoki M,Hirose T,Asakura K,Kataura A

    更新日期:1988-01-01 00:00:00

  • Tamoxifen as prophylaxis for prevention of gynaecomastia and breast pain associated with bicalutamide 150 mg monotherapy in patients with prostate cancer: a randomised, placebo-controlled, dose-response study.

    abstract:OBJECTIVE:To define the optimum tamoxifen dose for reducing bicalutamide (CASODEX) 150 mg monotherapy-induced breast events (ie, gynaecomastia or breast pain or both) without compromising disease control. METHODS:This was a double-blind, parallel-group, multicentre trial in which 282 patients with prostate cancer were...

    journal_title:European urology

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1016/j.eururo.2007.01.031

    authors: Fradet Y,Egerdie B,Andersen M,Tammela TL,Nachabe M,Armstrong J,Morris T,Navani S

    更新日期:2007-07-01 00:00:00

  • Celecoxib has potent antitumour effects as a single agent and in combination with BCG immunotherapy in a model of urothelial cell carcinoma.

    abstract:OBJECTIVES:Prostaglandin E2 (PGE2) is a potent immune modulator and known to suppress both tumour antigen-specific helper T (TH1) cells and the generation of cytotoxic T lymphocytes (CTLs). We hypothesised that a combination of the cyclooxygenase 2 (COX-2) selective inhibitor celecoxib and intravesical bacillus Calmett...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1016/j.eururo.2008.01.013

    authors: Dovedi SJ,Kirby JA,Davies BR,Leung H,Kelly JD

    更新日期:2008-09-01 00:00:00

  • Nanotechnology and its relevance to the urologist.

    abstract:OBJECTIVES:We review important aspects of nanotechnology, and discuss the wide range of research and clinical applications of nanomedicine in the field of urology. There is particular emphasis on key clinical and pre-clinical studies to provide an update on recent and potential applications in the care of urological pa...

    journal_title:European urology

    pub_type: 杂志文章,评审

    doi:10.1016/j.eururo.2007.04.065

    authors: Gommersall L,Shergill IS,Ahmed HU,Hayne D,Arya M,Patel HR,Hashizume M,Gill IS

    更新日期:2007-08-01 00:00:00

  • Survival and impact of clinical prognostic factors in surgically treated metastatic renal cell carcinoma.

    abstract:BACKGROUND:The survival impact of metastasectomy for metastatic renal cell carcinoma (mRCC) is still an active research field, particularly in the multimodal/targeted therapy era. OBJECTIVE:To determine the survival impact of clinical prognostic factors and their application to stratification of patients according to ...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1016/j.eururo.2012.09.037

    authors: Tosco L,Van Poppel H,Frea B,Gregoraci G,Joniau S

    更新日期:2013-04-01 00:00:00

  • EAU penile cancer guidelines 2009.

    abstract:CONTEXT:Squamous cell carcinoma (SCC) of the penis is a relatively rare but ominous disease. OBJECTIVE:To present a condensed version of the updated 2009 European Association of Urology (EAU) guidelines on penile SCC. EVIDENCE ACQUISITION:We performed a literature search of new data available up to December 2009. No ...

    journal_title:European urology

    pub_type: 杂志文章,实务指引

    doi:10.1016/j.eururo.2010.01.039

    authors: Pizzocaro G,Algaba F,Horenblas S,Solsona E,Tana S,Van Der Poel H,Watkin NA,European Association of Urology (EAU) Guidelines Group on Penile Cancer.

    更新日期:2010-06-01 00:00:00

  • DNA flow cytometry reveals depressed spermatogenetic activity in the contralateral testis within 24 h of ipsilateral spermatic cord torsion independently of the presence of the testis and epididymis.

    abstract::An experimental study was planned to evaluate if contralateral testicular deterioration following ipsilateral torsion requires the presence of a twisted ipsilateral testis and/or epididymis. Five groups, each containing 6 rats, were established. The groups underwent sham operation, epididymo-orchiectomy, testicular to...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1159/000019744

    authors: Salman AB,Okur DH,Tanyel FC

    更新日期:1998-10-01 00:00:00

  • Oncologic outcome after laparoscopic radical prostatectomy: 10 years of experience.

    abstract:BACKGROUND:While the published short-term oncologic outcomes after laparoscopic radical prostatectomy (LRP) are encouraging, intermediate and long-term data are lacking. OBJECTIVE:We analyzed the oncologic outcome after LRP based on 10 yr of experience. DESIGN, SETTING, AND PARTICIPANTS:This retrospective analysis of...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1016/j.eururo.2008.10.036

    authors: Touijer K,Secin FP,Cronin AM,Katz D,Bianco F,Vora K,Reuter V,Vickers AJ,Guillonneau B

    更新日期:2009-05-01 00:00:00

  • Reliability of clinical assessment of fluid absorption in transurethral prostatic resection.

    abstract::We evaluated 5 frequently used clinical methods of assessing absorption of irrigation fluid during 118 transurethral prostatic resections. The use of an irrigating solution containing glycine 1.5% and ethanol 1% and subsequent analysis of the alcohol concentration in the exhaled breath (ethanol monitoring) served as c...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1159/000474306

    authors: Olsson J,Rentzhog L,Hjertberg H,Hahn RG

    更新日期:1993-01-01 00:00:00

  • Management of renal angiomyolipoma: analysis of 15 cases.

    abstract:OBJECTIVE:We present our experience with 15 patients with renal angiomyolipoma warranting intervention. METHODS:The medical records and radiological studies were reviewed for patient age and sex, tumor location and size, association with tuberous sclerosis, and treatment approach. All patients were regularly followed ...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1159/000019658

    authors: Kessler OJ,Gillon G,Neuman M,Engelstein D,Winkler H,Baniel J

    更新日期:1998-01-01 00:00:00

  • Renal cysts. II. Chemical and dynamic study of cystic fluid.

    abstract::The chemical analysis of the fluid obtained by puncturing 100 simple renal cysts in adults show that the composition of cystic fluid resembles that of interstitial fluid. The chemical balance between cystic fluid and the interstitial space seems to occur passively. Antibiotics which were given orally (amoxycilline, mi...

    journal_title:European urology

    pub_type: 杂志文章

    doi:

    authors: Steg A

    更新日期:1976-01-01 00:00:00

  • A novel model of urinary tract differentiation, tissue regeneration, and disease: reprogramming human prostate and bladder cells into induced pluripotent stem cells.

    abstract:BACKGROUND:Primary culture and animal and cell-line models of prostate and bladder development have limitations in describing human biology, and novel strategies that describe the full spectrum of differentiation from foetal through to ageing tissue are required. Recent advances in biology demonstrate that direct repro...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1016/j.eururo.2013.03.054

    authors: Moad M,Pal D,Hepburn AC,Williamson SC,Wilson L,Lako M,Armstrong L,Hayward SW,Franco OE,Cates JM,Fordham SE,Przyborski S,Carr-Wilkinson J,Robson CN,Heer R

    更新日期:2013-11-01 00:00:00

  • European Association of Urology Guidelines on Non-muscle-invasive Bladder Cancer (TaT1 and Carcinoma In Situ) - 2019 Update.

    abstract:CONTEXT:This overview presents the updated European Association of Urology (EAU) guidelines for non-muscle-invasive bladder cancer (NMIBC), TaT1, and carcinoma in situ (CIS). OBJECTIVE:To provide practical recommendations on the clinical management of NMIBC with a focus on clinical presentation and recommendations. E...

    journal_title:European urology

    pub_type: 杂志文章,评审

    doi:10.1016/j.eururo.2019.08.016

    authors: Babjuk M,Burger M,Compérat EM,Gontero P,Mostafid AH,Palou J,van Rhijn BWG,Rouprêt M,Shariat SF,Sylvester R,Zigeuner R,Capoun O,Cohen D,Escrig JLD,Hernández V,Peyronnet B,Seisen T,Soukup V

    更新日期:2019-11-01 00:00:00

  • The influence of magnesium chloride on blood and urine parameters in calcium oxalate stone patients.

    abstract::The influence of magnesium chloride on various blood and urine parameters in calcium oxalate stone patients is studied. High dose magnesium therapy was found to increase urinary magnesium concentrations, whereas the oxalic acid concentration is reduced. The experiments support the statements on the role of magnesium i...

    journal_title:European urology

    pub_type: 杂志文章

    doi:10.1159/000473189

    authors: Brundig P,Berg W,Schneider HJ

    更新日期:1981-01-01 00:00:00