Abstract：
INTRODUCTION:Porphyromonas gingivalis and Tannerella forsythia are anaerobic bacteria commonly involved in root canal infections. Although previous investigations have assessed these species by strictly qualitative approaches, accurate determination of their cell levels by a sensitive quantitative technique may contribute with additional information regarding relevance in pain of endodontic origin. METHOD:The root canal levels of P. gingivalis, T. forsythia, and total bacteria were investigated by a quantitative polymerase chain reaction (PCR) assay based on unique copy molecular markers. A total of 32 symptomatic (n = 14) and asymptomatic (n = 18) cases of endodontic infections were analyzed. Root canal samples were collected; genomic DNA was extracted and submitted to SYBR Green I real-time PCR targeting the rgpB (P. gingivalis), bspA (T. forsythia), and rpoB (total bacteria) single copy genes. RESULTS:Overall, P. gingivalis, T. forsythia, and the coexistence of both species were encountered in 28%, 66%, and 22% of the subjects, respectively. P. gingivalis and T. forsythia levels ranged from 5.65 x 10(-6) to 1.20 x 10(-2) and from 5.76 x 10(-6) to 1.35 x 10(-1). T. forsythia was highly prevalent and numerous in the study groups, whereas P. gingivalis was moderately frequent and less abundant, displaying 19-fold lower average levels than the former. CONCLUSIONS:The endodontic levels of P. gingivalis and T. forsythia, individually or in conjunction, did not display significant associations with the manifestation of pain of endodontic origin.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Saito D,Coutinho LL,Borges Saito CP,Tsai SM,Höfling JF,Gonçalves RBdoi
10.1016/j.joen.2009.08.005subject
Has Abstractpub_date
2009-11-01 00:00:00pages
1518-24issue
11eissn
0099-2399issn
1878-3554pii
S0099-2399(09)00679-7journal_volume
35pub_type
杂志文章abstract：:The purpose of this study was to evaluate a new periapical index based on cone beam computed tomography (CBCT) for identification of apical periodontitis (AP). The periapical index proposed in this study (CBCTPAI) was developed on the basis of criteria established from measurements corresponding to periapical radioluc...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.08.013
更新日期：2008-11-01 00:00:00
abstract：:This study compared the weight and radiographic appearance of Ca(OH)(2) delivered into standardized, minimally and fully prepared canals using (a) an injection system, (b) an endodontic Flex-O file rotated counter-clockwise, (c) a lentulo spiral, and (d) a .04 rotary NiTi file rotated in reverse. Two extracted human s...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.01.009
更新日期：2006-07-01 00:00:00
abstract：:Nickel-titanium engine-driven rotary instruments are used increasingly in endodontic practice. One frequently mentioned problem is fracture of an instrument in the root canal. Very few studies have been conducted on torsional characteristics of these instruments, and none has been done under dynamic conditions. The pu...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200003000-00007
更新日期：2000-03-01 00:00:00
abstract：:A comparison was made of the apical leakage of three sealers. Fifty single-root human teeth were randomly divided into 5 groups (n = 10; 3 experimental and 2 control). The teeth of the positive-control and experimental groups were instrumented with K-type files to size 45. The experimental groups were obturated by lat...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200206000-00001
更新日期：2002-06-01 00:00:00
abstract：INTRODUCTION:Complement activation is considered a major mechanism in innate immunity. Although it is mainly involved in initiating inflammation, recent data reported its involvement in other processes such as tissue regeneration. In the dental pulp, complement C5a fragment has been shown to be involved in the recruitm...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.06.011
更新日期：2016-09-01 00:00:00
abstract：INTRODUCTION:Various factors may influence intracanal calcification in teeth treated with regenerative endodontic procedures. Bioactive materials, including ProRoot MTA (MTA; Dentsply Tulsa Dental Specialties, Memphis, TN) and Biodentine (BD; Septodont, Saint-Maur-des-Fossés, France), have been widely used as a coronal...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.03.011
更新日期：2019-06-01 00:00:00
abstract：:The purpose of this study was to examine the effect of the smear layer on apical microleakage over 16 wk. One hundred and four extracted human teeth were assigned to four groups treated as follows: group A1--smear layer was left intact and canals were obturated with gutta-percha and Roth 811; group A2--smear layer was...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(99)80010-7
更新日期：1999-02-01 00:00:00
abstract：INTRODUCTION:The purpose of this retrospective study was to determine the success of the inferior alveolar nerve (IAN) block using either 3.6 mL or 1.8 mL 2% lidocaine with 1:100,000 epinephrine in patients presenting with symptomatic irreversible pulpitis. METHODS:As part of 7 previously published studies, 319 emerge...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.04.007
更新日期：2013-08-01 00:00:00
abstract：:It has been shown that rotary nickel-titanium files cannot be used indefinitely. Researchers and clinicians have not been able to agree on how many times a file can be used before being discarded, except if a file has fractured or become visibly distorted. This study used ISO size 20 files of 0.04 taper in the curved ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200202000-00014
更新日期：2002-02-01 00:00:00
abstract：:The aim of this study was to determine the relationship between histologic and radiologic signs of inflammation in human root-filled teeth. In addition, other factors with possible importance for apical inflammation were assessed. Fifty-three block sections of root-filled teeth were gathered from human cadavers. The b...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200402000-00003
更新日期：2004-02-01 00:00:00
abstract：:There is an increasing interest in the utility of dental pulp stem cells (DPSCs) for dentin regeneration. The mechanisms involved in DPSC differentiation remain poorly understood. The purpose of the study was to investigate the mineralization capacity of human dental pulp cells (DPCs) and identify potential markers fo...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.02.009
更新日期：2007-06-01 00:00:00
abstract：:Reports on the prevalence of Enterococcus faecalis in root canals vary considerably, potentially because of variations in clinical sampling and sample analysis methods. This study compared culture and real-time quantitative polymerase chain reaction (qPCR) to detect and quantify E. faecalis in the same root canal samp...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.10.037
更新日期：2006-03-01 00:00:00
abstract：:This study compared the cytotoxicity of MetaSEAL (Parkell Inc, Farmington, NY), a methacrylate resin-based sealer with an epoxy resin-based (AH Plus Jet; Dentsply Caulk, Milford, DE) and a zinc oxide-eugenol-based sealer (Pulp Canal Sealer; SybronEndo, Orange, CA). Five-millimeter diameter disks prepared from the resp...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.06.020
更新日期：2008-09-01 00:00:00
abstract：:Although many options are proposed for the treatment of cracked posterior teeth, most treatment decisions are not evidence based. Thus, considerable individual variation can occur regarding treatment recommendations for the same scenario. To our knowledge, there are no studies in the literature assessing practitioners...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.03.029
更新日期：2017-09-01 00:00:00
abstract：INTRODUCTION:In a clinical situation, an apically resected tooth is often accompanied by a varying degree of periodontal bone loss. The purpose of this study was to assess the influence of apical root resection combined with periodontal bone loss on the biomechanical response of a single-rooted tooth. METHODS:A basic ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.11.011
更新日期：2015-03-01 00:00:00
abstract：:The purpose of this study was to evaluate the number of canals and isthmuses obturated after Cr, Er: YSGG laser treatment. Fifty-two canals were instrumented to a size 40 Profile 0.06 file combined with RC-Prep and sodium hypochlorite, or sterile water. The teeth were divided into 3 groups: (I) canals were flooded wit...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2007.05.012
更新日期：2007-09-01 00:00:00
abstract：:Conventional and surgical endodontic management were performed on a two-rooted maxillary central incisor with a periapical lesion. Anatomical complexity of the tooth lead to perforation in the distal root during conventional treatment, thus a surgical approach was taken. A 4-month postoperative radiograph shows repair...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200306000-00011
更新日期：2003-06-01 00:00:00
abstract：INTRODUCTION:The aim of this prospective study was to evaluate the postoperative pain that followed root canal treatments performed with a single-file reciprocating system on asymptomatic uniradicular necrotic teeth with and without foraminal enlargements (FEs). METHODS:Forty-six volunteers were randomly divided into ...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2015.09.018
更新日期：2016-01-01 00:00:00
abstract：:Silicone tubes filled with CRCS, a calcium hydroxide-based cement, were implanted at two different localizations in the right tibias of white male Wistar rats. A similar number of solid silicone rods were implanted in the left tibias of the same animals and were used as inert controls. Fifteen days after implantation ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(89)80090-1
更新日期：1989-01-01 00:00:00
abstract：:The cytotoxicity of four different calcium hydroxide-based root canal sealers (Sealapex, Apexit, CRCS, and Endoflas FS) was tested using human gingiva fibroblasts. The fibroblasts were acquired from the attached gingiva of apparently healthy patients. The sealers were allowed to set for 24 and 48 h before being covere...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81309-9
更新日期：1992-03-01 00:00:00
abstract：:A palatogingival groove is an anatomic malformation that predisposes the involved tooth to a severe periodontal defect. When the condition is complicated by pulpal necrosis, affected teeth often present a dilemma in terms of diagnosis and treatment planning. In this report, we describe the case of a patient with a max...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.10.009
更新日期：2016-02-01 00:00:00
abstract：INTRODUCTION:Communication between pulp and periodontal tissue has been well established. However, it is unknown when periodontal disease begins to affect the clinical response of pulp tissue. The aim of this study was to assess the influence of periodontal severity on pulp sensibility by means of electric and thermal ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.07.006
更新日期：2017-11-01 00:00:00
abstract：:The purpose of this in vitro study was to investigate the canal configuration types, and the prevalence and location of anatomical variations in the mesiobuccal (MB) and mesial roots of permanent maxillary and mandibular first molars after instrumentation. The number and the type of canals were determined before instr...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000145425.73364.91
更新日期：2005-05-01 00:00:00
abstract：INTRODUCTION:In this study, we investigated if apical periodontitis (AP) associated with diabetes influenced the levels of endogenous antioxidants, the total antioxidant capacity (TAC), and the oxidant parameter in the serum of Wistar rats. METHODS:Forty male rats were divided into 4 equal groups: normal rats (N), rat...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.05.014
更新日期：2017-10-01 00:00:00
abstract：:Occurrence of apical transportation (AT) may complicate the root filling procedure and result in a compromised seal. In part I of this study, human mandibular premolars with single, curved (21 to 39 degrees), or straight canals were prepared by Lightspeed or a step-back hand filing technique. An AT index was determine...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200004000-00003
更新日期：2000-04-01 00:00:00
abstract：:The purpose of this study was to compare the in vitro sealing capacity of five materials, each used as a temporary sealing agent for the walking bleach technique. All teeth received traditional biomechanical root canal instrumentation, after which the walking bleach agent was placed in the pulp chamber space. The occl...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200012000-00011
更新日期：2000-12-01 00:00:00
abstract：INTRODUCTION:The purpose of this prospective, randomized controlled trial was to evaluate the healing outcomes of platelet-rich fibrin (PRF) in periapical surgeries involving apicomarginal defects and to compare these results with surgeries not using any guided tissue regeneration techniques. METHODS:Thirty patients w...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2015.04.004
更新日期：2015-07-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate the effect of calcium hydroxide (CH) and antibiotic pastes, including a mixture of metronidazole and ciprofloxacin, with and without minocycline or cefaclor, on the dislocation resistance of mineral trioxide aggregate (MTA) to root dentin. METHODS:Eighty single-rooted...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.08.018
更新日期：2014-12-01 00:00:00
abstract：:Porphyromonas endodontalis (formerly Bacteroides endodontalis) is a black-pigmented anaerobic Gram-negative rod which is associated with endodontal infections. It has been isolated from infected dental root canals and submucous abscesses of endodontal origin. The presence of P. endodontalis in infected dental root can...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80843-5
更新日期：1992-09-01 00:00:00
abstract：:The aim of this study was to investigate the extent of calcium ion (Ca(2+)) removal on root dentin after treatment with 1.25% NaOCl, 2.5% NaOCl, 5.25% NaOCl, 2% chlorhexidine (CHX), 2% iodine potassium iodide (IKI), and MTAD in 3 immersion time periods. Extracted human mandibular premolars were bisected longitudinally...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.11.016
更新日期：2009-02-01 00:00:00
