Abstract：
:Recent evidence indicates that the N-methyl-D-aspartate (NMDA) receptor-channel complex contains a glycine subunit whose activation may be necessary for channel operation. It has been previously shown that stimulation of the NMDA receptor leads to an increase in intracellular ionic Ca2+ [( Ca2+]i); therefore, we examined the role of the NMDA receptor-associated glycine site in modulating [Ca2+]i using the fluorescent dye Fura II in hippocampal neuron cultures. A 3-s pulse of 200 microM NMDA resulted in a mean [Ca2+]i increase of 363 nM above the average resting concentration of 122 nM. Perfusion of the glycine site antagonist 7-chlorokynurenate (Cl-Kyn) essentially eliminated the NMDA-induced alteration in [Ca2+]i. Either 40 microM glycine or 50 microM D-serine completely reversed the effect of Cl-Kyn, indicating that the drug was acting at the glycine site. The NMDA receptor antagonists 2-amino-5-phosphonovalerate (AP5) and ketamine, which bind to the glutamate recognition site and the ion channel, respectively, also blocked the NMDA-mediated [Ca2+]i response; however, glycine or D-serine did not reverse this effect. These data show that the glycine binding site coupled to the NMDA receptor modulates the NMDA-mediated increase in [Ca2+]i. Antagonists of the glycine site provide a new tool to investigate and possibly control neuroplasticity and neurotoxicity related to the NMDA receptor complex.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Oliver MW,Shacklock JA,Kessler M,Lynch G,Baimbridge KGdoi
10.1016/0304-3940(90)90071-gsubject
Has Abstractpub_date
1990-07-03 00:00:00pages
197-202issue
2eissn
0304-3940issn
1872-7972pii
0304-3940(90)90071-Gjournal_volume
114pub_type
杂志文章abstract：:The irreversible labeling by [3H]flunitrazepam of three proteins with apparent molecular weights of 51,000 (P51), 55,000 (P55) and 59,000 (P59) was investigated using hippocampal membranes isolated from rats at various timepoints after birth. The present results indicate that [3H]flunitrazepam predominantly labels P55...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(82)90056-8
更新日期：1982-07-20 00:00:00
abstract：:Several studies have suggested that the activity of nitric oxide synthase (NOS) may be involved in the regulation of food intake in the genetically obese Zucker rats. In the present study, we investigated the expression of NOS in various hypothalamic regions of obese and lean Zucker rats using nicotinamide adenine din...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(00)01428-2
更新日期：2000-09-29 00:00:00
abstract：:The pathological basis for essential tremor (ET) is unknown. We used proton magnetic resonance spectroscopic imaging (1H MRSI) in 16 ET patients and 11 controls to measure levels of intracellular metabolites, including N-acetyl-L-aspartate (NAA) and total choline, relative to total creatine (tCR), in several brain reg...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(02)00966-7
更新日期：2002-11-15 00:00:00
abstract：:Depression associated with diabetes has been described as a highly debilitating comorbidity. Due to its complex and multifactorial mechanisms, the treatment of depression associated with diabetes represents a clinical challenge. Cannabidiol (CBD), the non-psychotomimetic compound derived from Cannabis sativa, has been...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2018.06.006
更新日期：2018-08-24 00:00:00
abstract：:Gangliosides are sialic acid-containing sphingolipids that have promoting effects on the survival, growth, and functions of neurons in the central nervous system. Glial cells including astrocytes assist neurons with regard to their proliferation, maintenance, and function. In the present study, we found that astrocyte...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2010.11.029
更新日期：2011-01-20 00:00:00
abstract：:The anatomical distribution of insulin-degrading enzyme (IDE) was studied in normal and Alzheimer's disease (AD) human brains. By use of a monospecific, polyclonal antiserum against the enzyme we identified IDE antigen in multiple cortical and subcortical neurons. Glia did not show IDE immunoreactivity. In AD brains i...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00135-4
更新日期：1999-03-26 00:00:00
abstract：:Normal cerebellar tissue, obtained from 15-day-old CBA/JNCij mouse embryos, was transplanted into the cerebellum of 4-week-old weaver mice. At the 6th week after the transplantation, the grafted tissue was distinguishable from the host cerebellum, developing a trilaminar organization. The formation of synapses by the ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(87)90437-x
更新日期：1987-08-31 00:00:00
abstract：:To test the hypothesis that the distribution of the pathology in variant Creutzfeldt-Jakob disease (vCJD) represents haematogenous spread of the disease, we studied the spatial correlation between the vacuolation, prion protein (PrP) deposits, and the blood vessel profiles in the cerebral cortex, hippocampus, dentate ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(03)00696-7
更新日期：2003-09-04 00:00:00
abstract：:Transmeatal cochlear laser (TCL) treatment has recently been proposed as a therapeutic procedure for cochlear dysfunction such as chronic cochlear tinnitus or sensorineural hearing loss. The aim of this study was to investigate whether TLC has any influence on the central nervous system using functional MRI with healt...
journal_title：Neuroscience letters
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.neulet.2006.08.049
更新日期：2007-01-16 00:00:00
abstract：:The antigenic components of Lewy bodies in the cerebral cortex and substantia nigra in 5 cases of diffuse Lewy body disease were examined by immunocytochemistry, using antibodies to neurofilaments (in the phosphorylated or non-phosphorylated forms); to ubiquitin; to the microtubule-associated proteins MAP1, MAP2 and t...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(89)90651-4
更新日期：1989-05-22 00:00:00
abstract：:The Rose Bengal model in rats is widely used to study brain plasticity, functional recovery and restorative therapies. The present study evaluated temporal profiles of hemorrhage and edema by magnetic resonance imaging (MRI) in rats in relation to sensorimotor impairment after photochemically induced cortical infarct....
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2007.09.043
更新日期：2007-11-27 00:00:00
abstract：:Gastrin-releasing peptide (GRP) was demonstrated by immunohistochemistry and high-performance liquid chromatography to be present in the great majority of neurons in the geniculate ganglion of rat and man. This was in contrast to findings in the sphenopalatine ganglion of rat, where only little GRP was found, and in t...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(92)90562-l
更新日期：1992-05-25 00:00:00
abstract：:One of the clinical characteristics of psychogenic tremors (PT) is the disruption or transient cessation of tremor with distractive manoeuvres, including those involving the performance of voluntary movements with the contralateral hand. Seven patients with PT, 11 patients with Parkinson's disease (PD), 10 patients wi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.08.009
更新日期：2004-11-11 00:00:00
abstract：:When standing quietly, human upright stance is typically approximated as a single segment inverted pendulum. In contrast, investigations which perturb upright stance with support surface translations or visual driving stimuli have shown that the body behaves like a two-segment pendulum, displaying both in-phase and an...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.11.071
更新日期：2005-03-29 00:00:00
abstract：:The act of tasting is the product of inseparable integrative behavior consisting of multi-sensory processing and orolingual motor coordination. Often tasting-induced brain activity is looked at in a reductionist manner as a set of isolated components. However, brain activity as a whole during tasting may not simply be...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.12.016
更新日期：2009-02-20 00:00:00
abstract：:Recent reports have suggested that variability in the alpha2-macroglobulin gene is a genetic risk factor for Alzheimer's disease. Here we have both tested a common polymorphism in the gene (I1000V) for association with the disease in a four-site case control study design, and tested the locus for linkage in a large se...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00035-x
更新日期：1999-03-05 00:00:00
abstract：:The retrotrapezoid nucleus (RTN), a part of the rostral ventrolateral medulla, is involved in the control of breathing. The mechanisms by which the RTN modulate the activity of respiratory neurons during chemoreceptor stimulation are not fully understood. This electrophysiological study performed in the cat demonstrat...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(00)00770-9
更新日期：2000-02-18 00:00:00
abstract：:A modified "cross-modal delayed response" paradigm was used to investigate putative processing of face expression in the absence of focused attention to the face. Neutral, happy and sad faces were presented during intervals occurring between a tone and a response imperative signal (a faint click), while subjects were ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2006.09.081
更新日期：2006-12-20 00:00:00
abstract：:The bradykinin B2 receptor (B2R) mediates many physiological processes such as hypotension, inflammation and blood-vessel permeability. Hypoxia/reoxygenation (H/R) induces neuronal cell apoptosis. It was found that B2R expression was enhanced in primary cultured cortical neurons after H/R treatment. Addition of bradyk...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.10.110
更新日期：2009-01-23 00:00:00
abstract：:The trembler mouse suffers from a dominantly inherited mutation of the peripheral myelin protein 22 (PMP22) gene which results in an abnormal myelination of its peripheral nervous system. The recent identification of two different PMP22 mRNA differing in their 5' non-translated region led us to monitor their respectiv...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)12008-r
更新日期：1995-10-06 00:00:00
abstract：:A FORTRAN computer program is described which enables solution of a set of partial differential equations describing synaptic transmission, including neurotransmitter diffusion, binding to receptors on the postsynaptic membrane with subsequent formation of open ion-conducting channels, and hydrolysis. Integration is p...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(84)90290-8
更新日期：1984-07-13 00:00:00
abstract：:Retinal action potential (RAP) was recorded at different depths in the median eye of scorpion, using a glass micropipette electrode (tip diameter 2-3 mum). The amplitude of RAP was largest when the tip of the electrode touched the distal process of the receptor cell (RC). The polarity of RAP became reversed when the t...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(77)90164-1
更新日期：1977-06-01 00:00:00
abstract：:Viral infections are frequently found in opioid addicts, subjecting them to immune challenge. However, the effects of immune challenge on opioid withdrawal are not fully understood. In the present study, mice were intraperitoneally injected with 2mg/kg polyinosinic-polycytidylic acid (Poly I:C, a viral mimetic) for 3 ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2010.10.052
更新日期：2011-01-10 00:00:00
abstract：:The involvement of protein kinases A and C in the induction of low frequency stimulation-induced long-term depression (LTD) in the medial perforant path of the dentate gyrus in vitro has been studied using the selective PKA inhibitors H-89 and KT 5720 and PKC inhibitors Bisindolylmaleimide and Ro-31-8220. The PKC inhi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00561-3
更新日期：1999-10-22 00:00:00
abstract：:From the effects of selective lesions it is concluded that opiate receptors are located on dopamine (DA) cell-bodies and/or dendrites as well as on GABA nerve-terminals in substantia nigra. ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(79)96056-7
更新日期：1979-05-01 00:00:00
abstract：:The nucleus raphe magnus belongs to the thermoafferent system. Following iontophoretic choleratoxin b injections in its rostral part, a substantial to large number of anterogradely labeled varicose fibres were observed in the medial and lateral preoptic areas, the bed nucleus, the substantia innominata, the ventral pa...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(96)13013-5
更新日期：1996-10-04 00:00:00
abstract：:Intra-arterial (i.a.) injection of Indian red scorpion (Mesobuthus tamulus; BT) venom produces cardiorespiratory changes by involving perivascular receptors. The afferents involved in mediating these reflex responses are not known. The present investigation was conducted to examine the afferents mediating the vasosens...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.04.037
更新日期：2008-06-13 00:00:00
abstract：:Cholinergic influences on attention are well documented and recent evidence indicates that genetic variation in the alpha4beta2 nicotinic receptor affects attentional networks of the brain. Several behavioral and electrophysiological studies have shown that a common polymorphism in the CHRNA4 gene (rs1044396) affects ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2009.02.029
更新日期：2009-04-10 00:00:00
abstract：:Convulsions were induced in rats by a single injection of pentylenetetrazol (PTZ) and [3H]L-2-amino-4-phosphonobutyrate ([3H]L-AP4) in vitro receptor autoradiography was used to evaluate the effects on the expression of group III metabotropic glutamate receptors. Significant increases by 60-80% in [3H]L-AP4 binding wa...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00235-9
更新日期：1999-04-30 00:00:00
abstract：:In freely moving rats, effects of unilateral haloperidol injection into the substantia nigra were monitored with in vivo voltammetry in the bilateral striata. The electrochemical responses at 120 mV versus Ag-AgCl, reflecting mainly a level of 3,4-dihydroxyphenylacetic acid (DOPAC), increased both in the striata withi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(84)90496-8
更新日期：1984-09-07 00:00:00
