Procentriole elongation and recruitment of pericentriolar material are downregulated in cyst cells as they enter quiescence.

Abstract:

:The apical region of the Drosophila testis contains a niche with two stem cell populations: germline stem cells (GSCs) and cyst progenitor cells (CPCs). Asymmetrical division of these stem cells leads to gonioblast daughters (which undergo further mitoses) and cyst cell daughters (which withdraw from the cell cycle and become quiescent). Although a considerable body of evidence indicates important roles for centrosomes in spindle orientation and asymmetrical division of GSCs, the behaviour and function of the centrioles in CPCs and their daughters remain unknown. Here, we show that quiescent cyst cells lose centrosome components after two divisions of the spermatogonia they envelop, but keep the centriolar component SAS-6. Cyst cells do have centriole pairs, but they are formed by a mother and a very short daughter that does not elongate or mature. The presence of procentrioles in quiescent cyst cells suggests that the centriole duplication cycle is uncoupled from the G1-S transition and that it might begin even earlier, in mitosis. Failure to enter the cell cycle might result in the improper recruitment of centriolar components at the mother centriole, thus hampering the full elongation of its daughter. Procentriole maturation defects could thus lead to the inability to maintain centrosomal components during development.

journal_name

J Cell Sci

journal_title

Journal of cell science

authors

Riparbelli MG,Colozza G,Callaini G

doi

10.1242/jcs.049957

subject

Has Abstract

pub_date

2009-10-15 00:00:00

pages

3613-8

issue

Pt 20

eissn

0021-9533

issn

1477-9137

pii

jcs.049957

journal_volume

122

pub_type

杂志文章
  • Recruitment of antigenic gamma-tubulin during mitosis in animal cells: presence of gamma-tubulin in the mitotic spindle.

    abstract::It has been claimed repeatedly that gamma-tubulin is exclusively localized at the spindle poles in mitotic animal cells, where it plays a role in microtubule nucleation. In addition to this localization, we have observed a gamma-tubulin-specific staining of the mitotic spindle in several animal cells (human, kangaroo ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Lajoie-Mazenc I,Tollon Y,Detraves C,Julian M,Moisand A,Gueth-Hallonet C,Debec A,Salles-Passador I,Puget A,Mazarguil H

    更新日期:1994-10-01 00:00:00

  • Steric hindrance in the upper 50 kDa domain of the motor Myo2p leads to cytokinesis defects in fission yeast.

    abstract::Cytokinesis in many eukaryotes requires a contractile actomyosin ring that is placed at the division site. In fission yeast, which is an attractive organism for the study of cytokinesis, actomyosin ring assembly and contraction requires the myosin II heavy chain Myo2p. Although myo2-E1, a temperature-sensitive mutant ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.205625

    authors: Palani S,Srinivasan R,Zambon P,Kamnev A,Gayathri P,Balasubramanian MK

    更新日期:2018-01-04 00:00:00

  • Notch in mechanotransduction - from molecular mechanosensitivity to tissue mechanostasis.

    abstract::Tissue development and homeostasis are controlled by mechanical cues. Perturbation of the mechanical equilibrium triggers restoration of mechanostasis through changes in cell behavior, while defects in these restorative mechanisms lead to mechanopathologies, for example, osteoporosis, myopathies, fibrosis or cardiovas...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:10.1242/jcs.250738

    authors: Stassen OMJA,Ristori T,Sahlgren CM

    更新日期:2020-12-21 00:00:00

  • Electrical dimensions in cell science.

    abstract::Cells undergo a variety of physiological processes, including division, migration and differentiation, under the influence of endogenous electrical cues, which are generated physiologically and pathologically in the extracellular and sometimes intracellular spaces. These signals are transduced to regulate cell behavio...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.023564

    authors: McCaig CD,Song B,Rajnicek AM

    更新日期:2009-12-01 00:00:00

  • Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody.

    abstract::Using a reconstituted complex of profilin and skeletal muscle actin as an antigen, we generated a monoclonal mouse antibody against actin, termed 2G2. As revealed by immunoblots of proteolytic actin fragments and by pepscan analysis, the antibody recognises a nonsequential epitope on actin which is located within thre...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Gonsior SM,Platz S,Buchmeier S,Scheer U,Jockusch BM,Hinssen H

    更新日期:1999-03-01 00:00:00

  • Extra-mitochondrial localisation of frataxin and its association with IscU1 during enterocyte-like differentiation of the human colon adenocarcinoma cell line Caco-2.

    abstract::Friedreich's ataxia is a recessive neurodegenerative disease due to insufficient expression of the mitochondrial protein frataxin. Although it has been shown that frataxin is involved in the control of intracellular iron metabolism, by interfering with the mitochondrial biosynthesis of proteins with iron/sulphur (Fe/S...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.02516

    authors: Acquaviva F,De Biase I,Nezi L,Ruggiero G,Tatangelo F,Pisano C,Monticelli A,Garbi C,Acquaviva AM,Cocozza S

    更新日期:2005-09-01 00:00:00

  • Syndecan-4 mediates antithrombin-induced chemotaxis of human peripheral blood lymphocytes and monocytes.

    abstract::Antithrombin inhibits chemokine-induced migration of neutrophils by activating heparan sulfate proteoglycan-dependent signaling. Whether antithrombin affects migration of other types of leukocytes is not known. We investigated the effects of antithrombin on spontaneous and chemokine-triggered migration of lymphocytes ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Kaneider NC,Reinisch CM,Dunzendorfer S,Römisch J,Wiedermann CJ

    更新日期:2002-01-01 00:00:00

  • Ets family members induce lymphangiogenesis through physical and functional interaction with Prox1.

    abstract::Prox1 plays pivotal roles during embryonic lymphatic development and maintenance of adult lymphatic systems by modulating the expression of various lymphatic endothelial cell (LEC) markers, such as vascular endothelial growth factor receptor 3 (VEGFR3). However, the molecular mechanisms by which Prox1 transactivates i...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.083998

    authors: Yoshimatsu Y,Yamazaki T,Mihira H,Itoh T,Suehiro J,Yuki K,Harada K,Morikawa M,Iwata C,Minami T,Morishita Y,Kodama T,Miyazono K,Watabe T

    更新日期:2011-08-15 00:00:00

  • Three-dimensional geometry of motile hamster spermatozoa.

    abstract::Micrographs were made of free-swimming hamster spermatozoa using a high intensity xenon flash and two-colour darkground illumination - conditions that permit three-dimensional reconstruction of the instantaneous shape of the flagellum. From the waveforms observed, we constructed an account of the three-dimensional kin...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Woolley DM,Osborn IW

    更新日期:1984-04-01 00:00:00

  • Mechanisms underlying p53 regulation of PIK3CA transcription in ovarian surface epithelium and in ovarian cancer.

    abstract::Inactivation of the transcription factor and tumor suppressor p53, and overexpression or mutational activation of PIK3CA, which encodes the p110alpha catalytic subunit of phosphatidylinositol-3-kinase (PI3K), are two of the most common deleterious genomic changes in cancer, including in ovarian carcinomas. We investig...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.013029

    authors: Astanehe A,Arenillas D,Wasserman WW,Leung PC,Dunn SE,Davies BR,Mills GB,Auersperg N

    更新日期:2008-03-01 00:00:00

  • Centriolar cycle of fused cells.

    abstract::Ultrastructure of centrioles of fused cells containing heterophasic interphase nuclei, premature chromosome condensation, and a telophase-like nucleus were studied. The study indicated that the heterophasic cellular environments contributed by the different cell partners exert a mutually opposite effect on the structu...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Manandhar G,Onishchenko GE

    更新日期:1995-02-01 00:00:00

  • Novel function of beta-arrestin2 in the nucleus of mature spermatozoa.

    abstract::A growing number of proteins originally found in endocytic structures of the plasma membrane appear to be able to traffic into the nucleus, but the cellular function of this translocation remains unclear. We have found that beta-arrestin2, which typically shows a cytoplasmic localization owing to constitutive nuclear ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.03046

    authors: Neuhaus EM,Mashukova A,Barbour J,Wolters D,Hatt H

    更新日期:2006-08-01 00:00:00

  • Vimentin contributes to human mammary epithelial cell migration.

    abstract::Vimentin expression in human mammary epithelial MCF10A cells was examined as a function of their migratory status using an in vitro wound-healing model. Analysis of the trajectories of the cells and their migratory speeds by time lapse-video microscopy revealed that vimentin mRNA and protein expression were exclusivel...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Gilles C,Polette M,Zahm JM,Tournier JM,Volders L,Foidart JM,Birembaut P

    更新日期:1999-12-01 00:00:00

  • Fe-S cluster coordination of the chromokinesin KIF4A alters its subcellular localization during mitosis.

    abstract::Fe-S clusters act as co-factors of proteins with diverse functions, for example, in DNA repair. Downregulation of the cytosolic iron-sulfur protein assembly (CIA) machinery promotes genomic instability through the inactivation of multiple DNA repair pathways. Furthermore, CIA deficiencies are associated with so far un...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.211433

    authors: Ben-Shimon L,Paul VD,David-Kadoch G,Volpe M,Stümpfig M,Bill E,Mühlenhoff U,Lill R,Ben-Aroya S

    更新日期:2018-06-27 00:00:00

  • Actin filament turnover removes bundles from Drosophila bristle cells.

    abstract::Drosophila bristle cells form enormous extensions that are supported by equally impressive scaffolds of modular, polarized and crosslinked actin filament bundles. As the cell matures and support is taken over by the secreted cuticle, the actin scaffold is completely removed. This removal begins during cell elongation ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Guild GM,Connelly PS,Vranich KA,Shaw MK,Tilney LG

    更新日期:2002-02-01 00:00:00

  • The endonuclease Ankle1 requires its LEM and GIY-YIG motifs for DNA cleavage in vivo.

    abstract::The LEM domain (for lamina-associated polypeptide, emerin, MAN1 domain) defines a group of nuclear proteins that bind chromatin through interaction of the LEM motif with the conserved DNA crosslinking protein, barrier-to-autointegration factor (BAF). Here, we describe a LEM protein annotated in databases as 'Ankyrin r...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.098392

    authors: Brachner A,Braun J,Ghodgaonkar M,Castor D,Zlopasa L,Ehrlich V,Jiricny J,Gotzmann J,Knasmüller S,Foisner R

    更新日期:2012-02-15 00:00:00

  • Cellular functions of WASP family proteins at a glance.

    abstract::Proteins of the Wiskott-Aldrich syndrome protein (WASP) family function as nucleation-promoting factors for the ubiquitously expressed Arp2/3 complex, which drives the generation of branched actin filaments. Arp2/3-generated actin regulates diverse cellular processes, including the formation of lamellipodia and filopo...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:10.1242/jcs.199570

    authors: Alekhina O,Burstein E,Billadeau DD

    更新日期:2017-07-15 00:00:00

  • Antennapedia homeobox as a signal for the cellular internalization and nuclear addressing of a small exogenous peptide.

    abstract::In a previous study we demonstrated that a homeobox peptide corresponding to the 60 amino acid long DNA-binding region of the Drosophila antennapedia homeo-protein was capable of crossing the plasma membrane of cells in culture. This finding has led us to investigate whether chimeric molecules encompassing the homeobo...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Perez F,Joliot A,Bloch-Gallego E,Zahraoui A,Triller A,Prochiantz A

    更新日期:1992-08-01 00:00:00

  • Formation of multivesicular endosomes in Dictyostelium.

    abstract::Multivesicular endosomes are present in virtually every eucaryotic cell, where they arise by intra-endosomal budding of the limiting endosomal membrane. Some genetic diseases such as Chediak-Higashi syndrome are characterized by enlarged membrane-filled endosomes. The same altered endosomal morphology can be observed ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.01524

    authors: Marchetti A,Mercanti V,Cornillon S,Alibaud L,Charette SJ,Cosson P

    更新日期:2004-12-01 00:00:00

  • Ultrastructure of the dinoflagellate Polykrikos. I. Development of the nematocyst-taeniocyst complex and morphology of the site for extrusion.

    abstract::Development of the nematocyst-taeniocyst complex in the four-zooid stage of a dinoflagellate, Polykrikos kofoidi, was studied by electron microscopy. We observed the following stages: formation of large spherical bodies in islets of cytoplasm containing extensive rough endoplasmic reticulum and Golgi complexes; differ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Westfall JA,Bradbury PC,Townsend JW

    更新日期:1983-09-01 00:00:00

  • Identification of PTPsigma as an autophagic phosphatase.

    abstract::Macroautophagy is a dynamic process whereby portions of the cytosol are encapsulated in double-membrane vesicles and delivered to the lysosome for degradation. Phosphatidylinositol-3-phosphate (PtdIns3P) is concentrated on autophagic vesicles and recruits effector proteins that are crucial for this process. The produc...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.080341

    authors: Martin KR,Xu Y,Looyenga BD,Davis RJ,Wu CL,Tremblay ML,Xu HE,MacKeigan JP

    更新日期:2011-03-01 00:00:00

  • Tumor necrosis factor-alpha (TNFalpha) regulates the epithelial barrier in the human intestinal cell line HT-29/B6.

    abstract::Cytokines are supposed to be mediators in diarrhoeal diseases. The aim of this study is to characterize the effect of tumor necrosis factor-alpha (TNFalpha) on epithelial barrier function in the colonic epithelial cell line HT-29/B6. Active ion transport and barrier function were measured as short-circuit current and ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Schmitz H,Fromm M,Bentzel CJ,Scholz P,Detjen K,Mankertz J,Bode H,Epple HJ,Riecken EO,Schulzke JD

    更新日期:1999-01-01 00:00:00

  • Integrin-matrix interactions affect the form of the structures developing from human mammary epithelial cells in collagen or fibrin gels.

    abstract::The HB2 cell line, developed from luminal epithelial cells cultured from milk, forms ball-like structures in collagen gels which show a uniform branching response to hepatocyte growth factor. The alpha2beta1 integrin is the major integrin expressed by luminal epithelial cells, and the role of this integrin in mammary ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Alford D,Baeckström D,Geyp M,Pitha P,Taylor-Papadimitriou J

    更新日期:1998-02-01 00:00:00

  • Reversible solidification of fission yeast cytoplasm after prolonged nutrient starvation.

    abstract::Cells depend on a highly ordered organisation of their content and must develop strategies to maintain the anisotropic distribution of organelles during periods of nutrient shortage. One of these strategies is to solidify the cytoplasm, which was observed in bacteria and yeast cells with acutely interrupted energy pro...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.231688

    authors: Heimlicher MB,Bächler M,Liu M,Ibeneche-Nnewihe C,Florin EL,Hoenger A,Brunner D

    更新日期:2019-11-08 00:00:00

  • Flow-dependent cellular mechanotransduction in atherosclerosis.

    abstract::Atherosclerosis depends on risk factors such as hyperlipidemia, smoking, hypertension and diabetes. Although these risk factors are relatively constant throughout the arterial circulation, atherosclerotic plaques occur at specific sites where flow patterns are disturbed, with lower overall magnitude and complex change...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.138313

    authors: Conway DE,Schwartz MA

    更新日期:2013-11-15 00:00:00

  • Effect of targeted expression of clusterin in photoreceptor cells on retinal development and differentiation.

    abstract::Clusterin expression is increased in tissues undergoing apoptosis, including neurodegenerative retina, but the causal relationships remain to be clarified. To test the hypothesis that overexpression of clusterin could induce apoptosis in neurons, transgenic mice were generated in which rat clusterin transgene was expr...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Jomary C,Chatelain G,Michel D,Weston A,Neal MJ,Jones SE

    更新日期:1999-05-01 00:00:00

  • LINCing the eukaryotic tree of life - towards a broad evolutionary comparison of nucleocytoplasmic bridging complexes.

    abstract::The nuclear envelope is much more than a simple barrier between nucleoplasm and cytoplasm. Nuclear envelope bridging complexes are protein complexes spanning both the inner and outer nuclear envelope membranes, thus directly connecting the cytoplasm with the nucleoplasm. In metazoans, they are involved in connecting t...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:10.1242/jcs.186700

    authors: Meier I

    更新日期:2016-10-01 00:00:00

  • The chemotactic and mitogenic effects of platelet-derived growth factor-BB on rat aorta smooth muscle cells are inhibited by basic fibroblast growth factor.

    abstract::In response to endovascular injury, platelet-derived growth factor-BB (PDGF-BB) and basic fibroblast growth factor (bFGF) are released locally and modulate vascular smooth muscle cells (SMC) proliferation and migration within the vascular wall. The aim of the present in vitro study was to determine how rat aorta SMC r...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Facchiano A,De Marchis F,Turchetti E,Facchiano F,Guglielmi M,Denaro A,Palumbo R,Scoccianti M,Capogrossi MC

    更新日期:2000-08-01 00:00:00

  • Contractility-dependent actin dynamics in cardiomyocyte sarcomeres.

    abstract::In contrast to the highly dynamic actin cytoskeleton in non-muscle cells, actin filaments in muscle sarcomeres are thought to be relatively stable and undergo dynamics only at their ends. However, many proteins that promote rapid actin dynamics are also expressed in striated muscles. We show that a subset of actin fil...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.046805

    authors: Skwarek-Maruszewska A,Hotulainen P,Mattila PK,Lappalainen P

    更新日期:2009-06-15 00:00:00

  • The kinesin-homologous protein encoded by the Chlamydomonas FLA10 gene is associated with basal bodies and centrioles.

    abstract::We previously reported that the FLA10 locus on the uni linkage group of Chlamydomonas encodes a kinesin homologous protein, KHP1. The fla10 phenotype, which is a temperature-sensitive defect for flagellar assembly and maintenance, is rescued by transformation with the wild-type KHP1 gene. In the present study we ident...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Vashishtha M,Walther Z,Hall JL

    更新日期:1996-03-01 00:00:00