Analysis of spindle characteristics and embryo quality in mice stimulated with letrozole using Polscope imaging.

Abstract:

OBJECTIVE:To explore the effect of letrozole as an ovulation inducing agent on oocyte and embryo quality in mice model. DESIGN:Prospective study. SETTING:Institute of Reproductive Medicine, Kolkata, India. ANIMAL(S):Sixty-nine sexually mature female Swiss Albino mice 6-7 weeks old. INTERVENTION(S):Metaphase II (MII) oocytes from two groups of mice, one group injected with letrozole and the other with rFSH. MAIN OUTCOME MEASURE(S):Number of MII oocytes, number of oocytes with meiotic spindle (MS), different angles of MS relative to the polar body (PB), spindle characteristics, and fertilization outcome. RESULT(S):The MS was present in 84% and 71.2% of the oocytes in the letrozole and rFSH group, respectively. In the letrozole group, 73% of the oocytes had a 0 degrees spindle position compared with 35.7% in the rFSH group. With letrozole, 19% of the oocytes had 0 degrees > MS < 90 degrees and 8% had MS >90 degrees , compared with 46.1% oocytes with 0 degrees > MS < 90 degrees and 18.2% with MS >90 degrees with rFSH. Mean spindle area retardance, spindle dimensions, and 4-8-cell embryo formation rate were significantly higher with letrozole compared with rFSH. Two-cell and blastocyst formation rates were similar in both groups. CONCLUSION(S):Robust birefringent spindles were obtained in mice on superovulation with letrozole. Letrozole does not appear to increase the risk of spindle assembly and preimplantation developmental arrest in mouse oocytes.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Ganesh A,Chattopadhyay R,Narendra Babu K,Chakravarty B,Chaudhury K

doi

10.1016/j.fertnstert.2009.04.045

subject

Has Abstract

pub_date

2010-03-15 00:00:00

pages

1477-81

issue

5

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(09)01013-9

journal_volume

93

pub_type

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