Chemical and electroporated transformation of Edwardsiella ictaluri using three different plasmids.

abstract：:Nocardioides sp. strain MTD22 degraded atrazine, ametryn and atraton, as did Arthrobacter aurescens strain TC1 and Nocardioides sp. strain C190. These strains contain trzN, a gene coding for TrzN, triazine hydrolase showing a broad substrate range. However, Nocardioides sp. strain AN3 degraded only atrazine despite co...

journal_title：FEMS microbiology letters

authors： Yamazaki K,Fujii K,Iwasaki A,Takagi K,Satsuma K,Harada N,Uchimura T

更新日期：2008-09-01 00:00:00

abstract：:The objective of this work was to analyze the effects of thermosonication (TS) on induction of a viable but non-culturable (VBNC) state in Salmonella Typhimurium and to examine incubation factors affecting subsequent resuscitation of cells. A TS treatment of 380 W at 53°C for 30 min induced the VBNC state in S. Typhim...

journal_title：FEMS microbiology letters

authors： Liao H,Jiang L,Zhang R

更新日期：2018-02-01 00:00:00

abstract：:A cDNA from Penicillium minioluteum HI-4 encoding a dextranase (1,6-alpha-glucan hydrolase, EC 3.2.1.11) was isolated and characterized. cDNA clones corresponding to genes expressed in dextran-induced cultures were identified by differential hybridization. Southern hybridization and restriction mapping analysis of sel...

journal_title：FEMS microbiology letters

authors： Garcia B,Margolles E,Roca H,Mateu D,Raices M,Gonzales ME,Herrera L,Delgado J

更新日期：1996-10-01 00:00:00

abstract：:A genomic bank was constructed in Escherichia coli HB101, consisting of DNA fragments from Bacteroides thetaiotaomicron strain 489 inserted within the vector pBR322. By screening on complex medium containing blue dextran, 10 stable dextranase-positive (Dex+) clones were isolated. Seven groups of Dex+ inserts were iden...

journal_title：FEMS microbiology letters

authors： Joncquiert JC,Béchet M,Tierny Y,Courtois J,Dubourguier HC,Guillaume JB

更新日期：1991-12-01 00:00:00

abstract：:Control of Helminthosporium solani, the cause of silver scurf in potato tubers, has been impaired by selection of benzimidazole-resistant strains as a result of repeated use of the fungicide thiabendazole. Identification of thiabendazole-resistant strains of H. solani by conventional techniques takes several weeks. Pr...

journal_title：FEMS microbiology letters

authors： McKay GJ,Cooke LR

更新日期：1997-07-15 00:00:00

abstract：:Serine hydroxymethyltransferase (SHMT) is a key enzyme in cellular one-carbon pathway and has been studied in many living organisms from bacteria to higher plants and mammals. However, biochemical and molecular characterization of SHMT from photoautotrophic microorganisms remains a challenge. Here, we isolated the SHM...

journal_title：FEMS microbiology letters

authors： Waditee-Sirisattha R,Sittipol D,Tanaka Y,Takabe T

更新日期：2012-08-01 00:00:00

abstract：:The formation of dormant endospores is a complex morphological process that permits long-term survival in inhospitable environments for many Gram-positive bacteria. Sporulation for the anaerobic gastrointestinal pathogen Clostridium difficile is necessary for survival outside of the gastrointestinal tract of its host....

journal_title：FEMS microbiology letters

authors： Edwards AN,McBride SM

更新日期：2014-09-01 00:00:00

abstract：:The effectiveness of maintaining prokaryotic RNA in Synechococcus and Pseudomonas cells, fixed in 96% ethanol, 4% paraformaldehyde, or suspended in RNAlater, and held in cold storage for 3 months was compared. Fluorometric determination of the RNA extracted from Synechococcus and Pseudomonas cells indicated that the c...

journal_title：FEMS microbiology letters

authors： Bachoon DS,Chen F,Hodson RE

更新日期：2001-07-24 00:00:00

abstract：:Haemin (iron protoporphyrin IX) is an essential growth factor for the periodontal pathogen. Porphyromonas gingivalis. Iron protoporphyrin IX (IPP IX) binding to the avirulent P. gingivalis beige variant (W50/BE1) and the black-pigmenting parent wild-type strain W50 was quantified. W50/BE1 grown in a chemostat under ha...

journal_title：FEMS microbiology letters

authors： Smalley JW,Birss AJ,McKee AS,Marsh PD

更新日期：1996-07-15 00:00:00

abstract：:The wine yeast Saccharomyces cerevisiae is central in the production of aroma compounds during fermentation. Some of the most important yeast-derived aroma compounds produced are esters. The esters ethyl acetate and isoamyl acetate are formed from alcohols and acetyl-CoA in a reaction catalysed by alcohol acetyltransf...

journal_title：FEMS microbiology letters

authors： Cordente AG,Swiegers JH,Hegardt FG,Pretorius IS

更新日期：2007-02-01 00:00:00

abstract：:A relatively simple, rapid sample preparation method has been developed for analysis of low-molecular mass compounds present in soluble coal products generated by microbial coal solubilizing agents. Acidification of the sample followed by direct extraction into hexanes is coupled with gas chromatography/mass spectrome...

journal_title：FEMS microbiology letters

authors： Toth-Allen J,Torzilli AP,Isbister JD

更新日期：1994-03-01 00:00:00

abstract：:Arbuscular mycorrhizal fungi (AMF) are a key soil functional group, with an important potential to increase crop productivity and sustainable agriculture including food security. However, there is clear evidence that land uses, crop rotations and soil features affect the AMF diversity and their community functioning i...

journal_title：FEMS microbiology letters

authors： Ontivero RE,Voyron S,Allione LVR,Bianco P,Bianciotto V,Iriarte HJ,Lugo MA,Lumini E

更新日期：2020-07-01 00:00:00

abstract：:A chitinase was purified from the cytosolic fraction of the anaerobic rumen fungus Piromyces communis OTS1 by affinity chromatography using regenerated chitin, gel filtration and chromatofocusing. The chitinase was most active at pH 6.2 and at 60 degrees C in a 20-min assay. The molecular mass of the purified protein ...

journal_title：FEMS microbiology letters

authors： Sakurada M,Morgavi DP,Komatani K,Tomita Y,Onodera R

更新日期：1996-03-15 00:00:00

abstract：:Pseudomonas aeruginosa are known to have a wide physiological potential allowing them to constantly populate diverse environments leading to severe infections of humans such as septicemia, leg ulcers, and burn wounds. We set out to probe physiological characteristics of P. aeruginosa isolates from diabetic leg ulcers ...

journal_title：FEMS microbiology letters

authors： Buivydas A,Pasanen T,Senčilo A,Daugelavičius R,Vaara M,Bamford DH

更新日期：2013-06-01 00:00:00

abstract：:A gene fusion between the Saccharomyces cerevisiae actin gene promoter and the cDNA of the Fusarium solani f. sp. pisi pelA gene has been constructed. This expression cassette has been introduced into the industrial wine yeast strain T73. The resulting recombinant strain is able to secrete active PELA enzyme into the ...

journal_title：FEMS microbiology letters

authors： González-Candelas L,Cortell A,Ramon D

更新日期：1995-03-01 00:00:00

abstract：:The glycolytic pathway of the hyperthermophilic archaea that belong to the order Thermococcales (Pyrococcus, Thermococcus and Palaeococcus) differs significantly from the canonical Embden-Meyerhof pathway in bacteria and eukarya. This archaeal glycolysis variant consists of several novel enzymes, some of which catalyz...

journal_title：FEMS microbiology letters

authors： van de Werken HJ,Verhees CH,Akerboom J,de Vos WM,van der Oost J

更新日期：2006-07-01 00:00:00

abstract：:Mycobacteria belonging to the Mycobacterium tuberculosis complex have the ability to invade and replicate in non-phagocytic cells, an event that requires the presence of bacterial surface components capable of triggering a cell response and the subsequent internalization of the microorganism. In this study, we report ...

journal_title：FEMS microbiology letters

authors： Flesselles B,Anand NN,Remani J,Loosmore SM,Klein MH

更新日期：1999-08-15 00:00:00

abstract：:It is now well established that physicochemical properties of exopolysaccharides (EPS) can vary between strains of a given species and according to growth conditions. The EPS production of four strains of Lactobacillus bulgaricus was monitored during growth in milk and in a chemically defined media. All strains, inclu...

journal_title：FEMS microbiology letters

authors： Petry S,Furlan S,Waghorne E,Saulnier L,Cerning dagger J,Maguin E

更新日期：2003-04-25 00:00:00

abstract：:The development of a methodology to identify the origin of fecal pollution is important both for assessing the degree of risk posed to public health and for developing strategies to mitigate the environmental loading of pathogens associated with waterborne disease transmission. Five rep-PCR genomic fingerprinting meth...

journal_title：FEMS microbiology letters

authors： Mohapatra BR,Broersma K,Mazumder A

更新日期：2007-12-01 00:00:00

abstract：:The halophilic actinomycete genus Streptomonospora, forming a distinct branch in the 16S rRNA gene phylogenetic tree adjacent to the genera Nocardiopsis and Thermobifida, was first proposed by Cui et al. to accommodate the species type Streptomonospora salina. During a biodiversity and taxonomic study on halophilic fi...

journal_title：FEMS microbiology letters

authors： Zhi XY,Tang SK,Li WJ,Xu LH,Jiang CL

更新日期：2006-10-01 00:00:00

abstract：:When the amino acid-fermenting bacterium Clostridium aminophilum F was inoculated into media containing 1 microM monensin or a bacteriocin-like inhibitory substance (BLIS) from Butyrivibrio fibrisolvens JL5, the cultures lagged and growth was not observed for more than 12 h. The monensin- and BLIS-treated cultures eve...

journal_title：FEMS microbiology letters

authors： Rychlik JL,Russell JB

更新日期：2002-03-19 00:00:00

abstract：:Ferric uptake regulator (Fur) proteins are widely recognized as repressors that in many prokaryotes regulate a large number of genes involved in iron homeostasis and oxidative stress response. In our study, we were able to identify the complete sequence of the fur gene from Microcystis aeruginosa using inverse-polymer...

journal_title：FEMS microbiology letters

authors： Martin-Luna B,Hernandez JA,Bes MT,Fillat MF,Peleato ML

更新日期：2006-01-01 00:00:00

abstract：:In this study we have investigated the ability of nonencapsulated, nontypeable Haemophilus influenzae, NT477 to survive in the J774 mouse macrophage-like cell line. Viable, intracellular nontypeable H. influenzae could still be recovered from macrophages 72 h after phagocytosis. In contrast, H. influenzae strain Rd, a...

journal_title：FEMS microbiology letters

authors： Craig JE,Cliffe A,Garnett K,High NJ

更新日期：2001-09-11 00:00:00

abstract：:The outer membrane protein (OMP) profiles of four different strains of Bacteroides fragilis, as determined by Coomassie blue stained polyacrylamide gels, were compared after growth in broth culture and in the mouse peritoneal cavity. There was no induction of the expression of large quantities of novel OMP after growt...

journal_title：FEMS microbiology letters

authors： Patrick S,Lutton DA

更新日期：1990-09-01 00:00:00

abstract：:Three 16S rRNA-targeted oligonucleotide probes, namely, PSMg437 targeting several members of the genus Pseudomonas, Hlm474 targeting several members of the genus Halomonas, and Clw844 targeting several members of the genus Colwellia, were designed. The microbial community structure and nitrogen removal ability of nitr...

journal_title：FEMS microbiology letters

authors： Yoshie S,Noda N,Tsuneda S,Hirata A,Inamori Y

更新日期：2004-06-01 00:00:00

abstract：:Streptococcus mutans, the etiologic agent of dental caries in humans, is considered a dominating force in the oral microbiome due to its highly-evolved propensity for survival. The oral pathogen encodes an elaborate array of regulatory elements, including the carbon catabolite-responsive regulator, CcpA, a global regu...

journal_title：FEMS microbiology letters

authors： Lindsay EL,Faustoferri RC,Quivey RG

更新日期：2021-01-16 00:00:00

abstract：:The main determinant of the plant pathogen Erwinia chrysanthemi virulence is the production of extracellular enzymes, mainly pectate lyases. Adjacent to a pectate lyase encoding locus, we identified the gene rotA supposed to encode a folding catalyst. Overproduction of the protein and assay of activity using a synthet...

journal_title：FEMS microbiology letters

authors： Pissavin C,Hugouvieux-Cotte-Pattat N

更新日期：1997-12-01 00:00:00

abstract：:Many antibiotic-producing actinomycetes possess at least one ABC (ATP-binding cassette) transporter which forms part of the antibiotic biosynthetic pathway and in most cases confers resistance to the drug in an heterologous host. Three types of antibiotic ABC transporters have been so far described in producer organis...

journal_title：FEMS microbiology letters

authors： Méndez C,Salas JA

更新日期：1998-01-01 00:00:00

abstract：:This report describes Vibrio seventh pandemic island II (VSP-II) and three novel variants revealed by comparative genomics of 23 Vibrio cholerae strains and their presence among a large and diverse collection of V. cholerae isolates. Three VSP-II variants were reported previously and our results demonstrate the presen...

journal_title：FEMS microbiology letters

authors： Taviani E,Grim CJ,Choi J,Chun J,Haley B,Hasan NA,Huq A,Colwell RR

更新日期：2010-07-01 00:00:00

abstract：:Primers designed to conserved regions of botulinum and tetanus clostridial toxins were used to amplify DNA fragments from non-proteolytic Clostridium botulinum type F (202F) DNA using polymerase chain reaction technology. The fragments were cloned and the complete nucleotide sequence of the gene encoding type F toxin ...

journal_title：FEMS microbiology letters

authors： East AK,Richardson PT,Allaway D,Collins MD,Roberts TA,Thompson DE

更新日期：1992-09-15 00:00:00