Sediment genotoxicity in the Tietê River (São Paulo, Brazil): in vitro comet assay versus in situ micronucleus assay studies.

Abstract:

:The in vitro comet assay with the permanent fish cell line RTL-W1 and the in situ micronucleus assay using erythrocytes from indigenous tilapia (Oreochromis niloticus) were used to detect genotoxicity in Tietê River sediments (São Paulo, Brazil). Either test was successful in identifying site-specific differences in genotoxicity, with a high correlation between in situ and in vitro results indicating the relevance of the latter even for environmental studies. Discharges from São Paulo city have major impact on genotoxic effects by sediment-bound contaminants; however, overall genotoxicity decreases downstream. The high genotoxic burden of the Tietê River warrants measures to reduce the input of toxic effluents.

journal_name

Ecotoxicol Environ Saf

authors

Rocha PS,Luvizotto GL,Kosmehl T,Böttcher M,Storch V,Braunbeck T,Hollert H

doi

10.1016/j.ecoenv.2009.04.013

subject

Has Abstract

pub_date

2009-10-01 00:00:00

pages

1842-8

issue

7

eissn

0147-6513

issn

1090-2414

pii

S0147-6513(09)00090-6

journal_volume

72

pub_type

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