Abstract:
AIM OF STUDY:Solanum lyratum herba (SLH) has been traditionally used for the treatment of febrifuge, diarrhea, eye disease and cancer with little scientific evidences. Thus, in the present study, to elucidate the antitumor mechanism of SLH: in vitro and in vivo experiments were performed with hexane fraction of Solanum lyratum herba (HSLH). MATERIALS AND METHODS:Cytotoxicity assay, 4'-6-diamidino-2-phenylindole (DAPI) staining, flow cytometric analysis for sub-G1 peaks, Western blot analysis were used with the antibodies of apoptosis related proteins in vitro. In addition, the effect of HSLH on in vivo tumor growth was evaluated in Lewis lung carcinoma (LLC) tumor model and immunohistochemistry also was performed with terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining in tumor section. RESULTS:HSLH exhibited cytotoxicity against LLC cells most effectively among its solvent fractions. Ladder like DNA fragmentation and apoptotic features such as chromatin condensation and apoptotic bodies were observed in HSLH treated LLC cells by 4'-6-diamidino-2-phenylindole staining. HSLH also significantly increased sub-G(1) peaks, activated caspase-8, -9 and -3 proteins and cleaved poly(ADP-ribose) polymerase (PARP). Furthermore, HSLH increased the phosphorylation of extracellular signal-regulated kinase (ERK), transiently activated phospho-JNK (c-jun N-terminal kinase) and downregulated phospho-p38 MAPK. In addition, we have found for the first time HSLH treatment effectively suppressed the in vivo growth of LLC to up to approximately 30% of untreated control at 50mg/kg and significantly increased apoptotic expression in tumor section by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. Taken together, these findings strongly demonstrate that hexane fraction of Solanum lyratum herba exerts antitumor activity via caspase activation and MAPK regulation and can be effectively applied to lung cancer as a cancer chemopreventive agent.
journal_name
J Ethnopharmacoljournal_title
Journal of ethnopharmacologyauthors
Lee JH,Lee YH,Lee HJ,Lee HJ,Lee EO,Ahn KS,Shim BS,Bae H,Choi SH,Ahn KS,Baek NI,Kim DK,Kim SHdoi
10.1016/j.jep.2009.02.013subject
Has Abstractpub_date
2009-05-04 00:00:00pages
121-7issue
1eissn
0378-8741issn
1872-7573pii
S0378-8741(09)00084-1journal_volume
123pub_type
杂志文章abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Panax ginseng is one of the most well-known medicinal herbs in Korea and China, which has been used for treatment and prevention of cancer, obesity, diabetes, and cardiovascular diseases. Ginsenosides are the major components of P. ginseng, having a wide range of pharmacological activitie...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2016.08.039
更新日期:2016-12-24 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Chrysanthemum morifolium Ramat (Asteraceae) has (CM) long been used in Korean and Chinese traditional herbal medicines with numerous therapeutic applications. AIM OF THE STUDY:To evaluate the neuroprotective activities of Chrysanthemum morifolium (CM) extract against 1-methyl-4-phenylpri...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2009.09.017
更新日期:2009-12-10 00:00:00
abstract::A complex prescription (CP) used traditionally for the treatment of vitiligo was prepared from water extract of eight plant materials. To study the mechanism of this preparation its effect on gene expression of B-16 murine melanoma cells was estimated using differential display method (DDRT-PCR). The results showed th...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2004.03.021
更新日期:2004-06-01 00:00:00
abstract:AIM OF STUDY:Diabetes mellitus is frequently combined with vascular diseases, which are associated with the expression of vascular endothelial growth factor (VEGF). An approach that can reverse the induction of VEGF by hyperglycemia may potentially benefit the outcome of diabetic patients. Therefore, in the present stu...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.07.018
更新日期:2011-09-02 00:00:00
abstract::When bradykinin-induced contraction of the isolated rat ileum was tested in the presence of Aloe barbadensis Mill. (Liliaceae) gel (fraction F-1) and with the fraction obtained by precipitation of the F-1 with 55% ammonium sulfate (F-55), the maximal responses to bradykinin were reduced by 10 and 22%, respectively. Fu...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2004.03.030
更新日期:2004-07-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Based on traditional medicine, infusions of Bidens species (Asteraceae) have been successfully used in the treatment of acute and chronic enteritis. Additionally, ethnopharmacological reports demonstrating the gastrointestinal, gastroprotective, anti-inflammatory, antiulcerogenic and immu...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.112982
更新日期:2020-09-15 00:00:00
abstract::Saussurea costus (Falc.) Lipschitz, syn Saussurea lappa C.B. Clarke is a well known and important medicinal plant widely used in several indigenous systems of medicine for the treatment of various ailments, viz. asthma, inflammatory diseases, ulcer and stomach problems. Sesquiterpene lactones have been reported as the...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.jep.2006.12.033
更新日期:2007-04-04 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Ganoderma lucidum Polysaccharide (GLP),traditional Chinese medicine (TCM) active ingredient, has a long history and has good curative effects on radiation injury. However, the mechanism of GLP treating radiation injury has not been clearly elucidated. THE AIM OF THE STUDY:This study was ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.112814
更新日期:2020-08-10 00:00:00
abstract::In vitro and in vivo studies revealed that Malaysian medicinal plants, Piper sarmentosum, Andrographis paniculata and Tinospora crispa produced considerable antimalarial effects. Chloroform extract in vitro did show better effect than the methanol extract. The chloroform extract showed complete parasite growth inhibit...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(98)00135-4
更新日期:1999-03-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:FuFang ZhenZhu TiaoZhi (FTZ) is a hospitalized traditional Chinese medicine herbal formula with documented metabolic benefits. Polycystic ovary syndrome (PCOS) characterized by ovarian dysfunction and insulin resistance represents one of the most common endocrine disorders in close associ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.113587
更新日期:2021-03-25 00:00:00
abstract::Previously, we demonstrated that Moutan Cortex prevents acetaminophen (AAP)-induced cytotoxicity in vitro. The present study examined the protective effect of Moutan Cortex on AAP induced hepatotoxicity and the possible mechanisms underlying this effect in mice. When Montan Cortex was administered to ICR mice, followe...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2003.11.004
更新日期:2004-02-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Typha angustata is used in traditional Chinese medicine for a variety of clinical disorders. Its pharmacological actions include beneficial effects on hyperlipidemia and myocardial infarction, as well as labor-inducing and antibacterial effects. AIM OF THE STUDY:We investigated the mecha...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.12.038
更新日期:2012-02-15 00:00:00
abstract::In the legendary biographies of some Buddhist adepts from the 2nd- and 9th-centuries there are some clues which can be interpreted to reveal that the adepts were consuming psychedelic Amanita muscaria, 'fly agaric', mushrooms to achieve enlightenment. This secret ingredient in the alchemical elixir they used to attain...
journal_title:Journal of ethnopharmacology
pub_type: 历史文章,杂志文章
doi:10.1016/0378-8741(95)01292-l
更新日期:1995-10-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Magnolia officinalis bark is used in traditional Chinese medicine for the treatment of cough, colds, fever, chronic bronchitis and stomach ailments. AIM OF THE STUDY:To investigate therapeutic effects of polyphenol rich extract from M. officinalis bark (MPE) in influenza virus A-infected...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2010.11.074
更新日期:2011-03-08 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Yin-Chen-Hao Tang (YCHT) has been a very popular, hepatoprotective three-herb formula with an unclear chemical base. AIM OF THIS STUDY:To reveal the hepatoprotective chemical base of oral-dosed YCHT, we bridged the hepatic disposition of six compounds in vivo and their hepatoprotection i...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2019.02.022
更新日期:2019-05-23 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Mesembrine alkaloids are considered to be the primary active constituents of the South African medicinal plant Sceletium tortuosum (L.) N.E.Br. (Aizoaceae), and it is used as the dried or fermented aerial material from the plant, which is known as kanna (aka, channa, kougoed). Traditional...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.jep.2016.12.004
更新日期:2017-01-04 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Terminalia arjuna (Roxb. ex DC.) Wight & Arn. (T. arjuna) has been widely used in the traditional ayurvedic system of medicine as a cardioprotectant and for acute and chronic renal diseases supporting its ethnopharmacological use. AIM OF THE STUDY:The present study aimed at evaluating th...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2016.01.002
更新日期:2016-03-02 00:00:00
abstract::A bioguided separation of Lepechinia graveolens (Reg.) Epling. (Lamiaceae) for antioxidant activity was carried out. The ethyl acetate fraction (EAF) from the methanolic extract was chromatographed on Sephadex LH-20 affording five active fractions. The radical scavenging activity of each fraction, as well as that of t...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2004.05.017
更新日期:2004-09-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Ethnobotanical surveys are detecting an increasing frequency of exotic plant species in pharmacopeias, which has led researchers to investigate the role of such species in traditional medical systems. According to the diversification hypothesis, exotic species are included to complete pha...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2014.07.036
更新日期:2014-09-11 00:00:00
abstract::Aconitum species have been used in China as an essential drug in Traditional Chinese Medicine (TCM) for 2000 years. Reviewing the clinical application of Aconitum, their pharmacological effects, toxicity and detoxifying measures, herb-herb interactions, clinical taboos, famous herbal formulas, traditional and current ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.jep.2009.07.031
更新日期:2009-10-29 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:It is said that black tea is effective against type 2 diabetes mellitus because it can help modulate postprandial hyperglycemia. However, the mechanism underlying its therapeutic and preventive effects on type 2 diabetes mellitus is unclear. In this study, we focused on the effect of blac...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2014.12.009
更新日期:2015-02-23 00:00:00
abstract:ETHNOPHARMOCOLOGICAL RELEVANCE:Cortex Eucommiae (CE), the bark of Eucommia ulmoides Oliv., has been traditionally used for its kidney-tonifying and bone- and tendon-enhancing properties in Korea, China, and Japan. CE has been historically prescribed for inflammatory conditions such as arthritis of the knee and ankle. ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2017.08.001
更新日期:2017-09-14 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Ficus is an important commercial crop not only for its nutritive value but also, for its medicinal value. Several Ficus species have been traditionally used in the Egypt, Indian and Chinese as carminative, astringent, antibacterial, hepatoprotective, and hypolipidemic agents. AIM OF THE ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2018.11.026
更新日期:2019-03-01 00:00:00
abstract::Chronic users of non-steroidal anti-inflammatory drugs frequently develop ulcerative lesions in their intestines. The purpose of the present study was to investigate whether eupatilin, an active ingredient derived from Artemisia plants, prevents this side effect in vitro. Extracts of the whole herb of Artemisia asiati...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2008.03.010
更新日期:2008-06-19 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Labisa pumila (LP), popularly known with its local name, Kacip Fatimah, is a well known herb grown in Indochina and Southeast Asia and is traditionally used to regain energy after giving birth in women. The propensity of LP to cause drug-herb interaction via cytochrome P450 (CYP) enzyme s...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2012.07.024
更新日期:2012-09-28 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Medicinal plants possessing abortifacient activity have been used traditionally for a long time in folk medicine. Anthocephalus cadamba, is one such herb that has been known to possess abortifacient potential in ethnobotanical literature, but has not been validated scientifically. MATERI...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2015.07.031
更新日期:2015-09-15 00:00:00
abstract:BACKGROUND AND PURPOSE:Based on ethno-botanical information collected from diabetic patients in Cuba and firstly reported inhibition of PTP1B and DPPIV enzymes activities, Allophylus cominia (A. cominia) was identified as possible source of new drugs that could be used for the treatment of type 2 diabetes mellitus (T2-...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2018.01.014
更新日期:2018-04-24 00:00:00
abstract::The effect of the aqueous extract of Retama raetam (RR) on blood glucose levels was investigated in fasting normal and streptozotocin-induced diabetic rats after single and repeated oral administration. The aqueous extract of RR at a dose of 20mg/kg significantly reduced the blood glucose in normal rats 6h after a sin...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(03)00104-1
更新日期:2003-07-01 00:00:00
abstract::A crude extract prepared from Scutellaria barbata D. Don (Lamiaceae) was analyzed in the effort to discover antibacterial compounds against high-level strains of methicillin-resistant Staphylococcus aureus (MRSA). Apigenin and luteolin were isolated from the plant as active constituents against the bacteria. These fla...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(00)00265-8
更新日期:2000-10-01 00:00:00
abstract::A room temperature aqueous extract of the roots of Taverniera abyssinica antagonized the contractile responses of the guinea-pig ileum to acetylcholine and histamine. The extract also relaxed the smooth muscle of the rabbit duodenum, abolished the pendular contractions and antagonized the effects of acetylcholine and ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/0378-8741(90)90021-k
更新日期:1990-08-01 00:00:00