Abstract:
:Despite huge improvements in neurobiological approaches for investigating the functional properties of neurotransmitter receptors and ion channels, many difficulties are still encountered when focusing on the human brain. Electrophysiological studies aimed at performing direct determinations on human nervous tissue are limited by neurosurgery and also by pathophysiological conditions prevailing before and after the resective operation. The electrophysiological study of receptors and channels becomes difficult also in animal models when the cells are not accessible and/or the experiments last many hours, during which the examined nervous tissue usually becomes unhealthy. To increase the possibility of doing optimal electrophysiological recordings, addressed to investigate the functional properties of receptors and channels, more than two decades ago, foreign mRNAs were injected into Xenopus oocytes to heterologously express the receptors; and about a decade ago cell membranes were injected into the oocytes to directly transplant the native receptors. While the first approach needs complex procedures for mRNA isolation, the membrane preparations are simpler to obtain and the embedded receptors are transplanted in their own membrane, with their own glycosylation and together with any ancillary proteins they may have. Using injections of membranes isolated from fresh nervous tissues several issues have already been addressed and many questions can be answered in the near future. Strikingly, with this approach it has been possible to "resuscitate" receptors and ion channels from tissues kept frozen for many years. This review focuses on recently obtained information and on some new lines of biological research using receptor microtransplantation into oocytes.
journal_name
Prog Neurobioljournal_title
Progress in neurobiologyauthors
Eusebi F,Palma E,Amici M,Miledi Rdoi
10.1016/j.pneurobio.2009.01.008subject
Has Abstractpub_date
2009-05-01 00:00:00pages
32-40issue
1eissn
0301-0082issn
1873-5118pii
S0301-0082(09)00022-7journal_volume
88pub_type
杂志文章abstract::The spinocerebellar ataxias type 1 (SCA1), 2 (SCA2), 3 (SCA3), 6 (SCA6) and 7 (SCA7) are genetically defined autosomal dominantly inherited progressive cerebellar ataxias (ADCAs). They belong to the group of CAG-repeat or polyglutamine diseases and share pathologically expanded and meiotically unstable glutamine-encod...
journal_title:Progress in neurobiology
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更新日期:2013-05-01 00:00:00
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journal_title:Progress in neurobiology
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journal_title:Progress in neurobiology
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journal_title:Progress in neurobiology
pub_type: 杂志文章,评审
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journal_title:Progress in neurobiology
pub_type: 杂志文章,评审
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journal_title:Progress in neurobiology
pub_type: 杂志文章,评审
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journal_title:Progress in neurobiology
pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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journal_title:Progress in neurobiology
pub_type: 杂志文章,评审
doi:10.1016/s0301-0082(98)00029-x
更新日期:1998-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-11-01 00:00:00
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journal_title:Progress in neurobiology
pub_type: 杂志文章,评审
doi:10.1016/j.pneurobio.2012.08.002
更新日期:2012-11-01 00:00:00
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journal_title:Progress in neurobiology
pub_type: 杂志文章,评审
doi:10.1016/j.pneurobio.2011.06.001
更新日期:2011-09-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1016/j.pneurobio.2012.04.003
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journal_title:Progress in neurobiology
pub_type: 杂志文章,评审
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journal_title:Progress in neurobiology
pub_type: 杂志文章,评审
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journal_title:Progress in neurobiology
pub_type: 杂志文章,评审
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更新日期:2011-10-01 00:00:00
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journal_title:Progress in neurobiology
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更新日期:2014-01-01 00:00:00
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journal_title:Progress in neurobiology
pub_type: 杂志文章,评审
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journal_title:Progress in neurobiology
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更新日期:2002-08-01 00:00:00
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更新日期:2017-06-01 00:00:00
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