Abstract：
OBJECTIVE:To investigate the effects of the non-cytotoxic drug (cyclooxygenase-2 (COX-2) inhibitor and octreotide) on growth of the multidrug-resistant human gastric carcinoma cell line SGC-7901/ADR. METHODS:The effects of non-cytotoxic drug on the growth of SGC-7901 and SGC-7901/ADR cells were evaluated by (3)H-thymidine incorporation assay. The apoptosis of cells was measured by the TdT-mediated dUTP nick end-labeling assay (TUNEL) and flow cytometric assay. Western blotting was used to analysis the expression of cyclooxygenase (COX-2) protein in SGC-7901 cells and SGC-7901/ADR cells and P-glycoprotein (P-gp) from SGC-7901/ADR cells with variable treatments. Activator protein-1 binding activity was examined by electrophoretic mobility shift assay. RESULTS:(3)H-thymidine incorporation into SGC-7901/ADR cells treated with celecoxib was significantly lower than that of control group (471.3 +/- 79.7 cpm vs 917.5 +/- 130.8 cpm, P < 0.05). When combined with octreotide, celecoxib presented lower (3)H-thymidine incorporations than its used alone and decreased to 53.3% of that amount original. Either celecoxib or the combination group markedly induced apoptosis in SGC-7901/ADR cells. COX-2 protein in the SGC-7901/ADR cells was higher than in that of the SGC-7901 cells (1.543 +/- 0.052 vs 0.564 +/- 0.021, P < 0.05). The inhibition of P-gp could be achieved with celecoxib alone and combination with octreotide (0.486 +/- 0.012, 0.252 +/- 0.014 vs 0.941 +/- 0.033, P < 0.05). Moreover, AP-1 binding activity could be suppressed by non-cytotoxic drug and showed a synergistic effect. CONCLUSION:The combination of non-cytotoxic drug significantly improved the inhibitive effects on the growth of multidrug-resistant human gastric cancer cells.
journal_name
J Dig Disjournal_title
Journal of digestive diseasesauthors
Wang CH,Zheng WB,Qiang O,Tang CWdoi
10.1111/j.1751-2980.2009.00370.xsubject
Has Abstractpub_date
2009-05-01 00:00:00pages
91-8issue
2eissn
1751-2972issn
1751-2980pii
CDD370journal_volume
10pub_type
杂志文章abstract：OBJECTIVE:Nonalcoholic fatty liver disease (NAFLD) is a common chronic liver disease, the natural course of which has not been well documented. This study aimed to perform a prospective cohort study to investigate NAFLD in a Chinese population. METHODS:Using our previous epidemiological survey, 3543 patients were foll...
journal_title：Journal of digestive diseases
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abstract：OBJECTIVE:Early diagnosis of colitis-associated cancer and dysplasia through surveillance endoscopy is vital for patients with ulcerative colitis (UC). This study aimed to evaluate the efficacy of autofluorescence endoscopy (AFE) using 5-aminolevulinic acid (ALA) and to investigate the fluorescence signal localization ...
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journal_title：Journal of digestive diseases
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doi：10.1111/j.1751-2980.2011.00502.x
更新日期：2011-06-01 00:00:00
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doi：10.1111/1751-2980.12415
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pub_type： 杂志文章,评审
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更新日期：2014-01-01 00:00:00
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journal_title：Journal of digestive diseases
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doi：10.1111/j.1751-2980.2009.00400.x
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abstract：:Acute non-variceal upper gastrointestinal bleeding (ANVUGIB) is one of the most common medical emergencies in China and worldwide. In 2009, we published the "Guidelines for the diagnosis and treatment of acute non-variceal upper gastrointestinal bleeding" for the patients in China; however, during the past years numer...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,实务指引
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journal_title：Journal of digestive diseases
pub_type： 杂志文章,随机对照试验
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doi：10.1111/1751-2980.12452
更新日期：2017-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/1751-2980.12055
更新日期：2013-07-01 00:00:00
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journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1751-2980.2008.00346.x
更新日期：2008-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1443-9573.2007.00271.x
更新日期：2007-02-01 00:00:00
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journal_title：Journal of digestive diseases
pub_type： 杂志文章,评审
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journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12053
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journal_title：Journal of digestive diseases
pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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更新日期：2015-07-01 00:00:00
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journal_title：Journal of digestive diseases
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更新日期：2017-08-01 00:00:00
abstract：OBJECTIVE:We aimed to identify the differences in symptoms, high-resolution manometry (HRM) characteristics, and the efficacy of peroral endoscopic myotomy (POEM) regarding patients' sex in achalasia. METHODS:All patients diagnosed with achalasia by HRM who underwent POEM and were followed up for more than 6 months we...
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doi：10.1111/1751-2980.12922
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journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1751-2980.2009.00388.x
更新日期：2009-08-01 00:00:00
abstract：OBJECTIVE:The efficacy of argon plasma coagulation (APC) on gastric antral vascular ectasia (GAVE) may be impaired over time and depends greatly on the application settings. Endoscopic band ligation (EBL) may be an alternative, but study on its efficacy is limited. This study aimed to evaluate and compare the clinical ...
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pub_type： 杂志文章
doi：10.1111/1751-2980.12958
更新日期：2021-01-01 00:00:00
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journal_title：Journal of digestive diseases
pub_type： 杂志文章
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更新日期：2019-09-01 00:00:00
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journal_title：Journal of digestive diseases
pub_type： 杂志文章
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更新日期：2017-03-01 00:00:00
abstract：OBJECTIVE:To evaluate the long-term outcomes of complete common bile duct (CBD) stone removal and biliary stenting in elderly patients (≥85 years) with CBD stones. METHODS:We retrospectively examined 65 patients who underwent complete CBD stone removal (the duct clearance group) and 40 patients who underwent biliary s...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12656
更新日期：2018-10-01 00:00:00
abstract：OBJECTIVE:Transarterial chemoembolization (TACE) is the treatment modality for intermediate, or Barcelona Clinic Liver Cancer stage B, hepatocellular carcinoma (HCC), but its beneficial effect on outcomes is still unsatisfactory. This study aimed to assess the outcomes of combined TACE and sorafenib for patients with i...
journal_title：Journal of digestive diseases
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