Abstract:
BACKGROUND:Helicobacter pylori has been strongly associated with chronic gastritis, peptic and duodenal ulcers, and it is a risk factor for gastric cancer. Three major virulence factors of H. pylori have been described: the vacuolating toxin (VacA), the cytotoxin-associated gene product (CagA) and the adhesion protein BabA2. Since considerable geographic diversity in the prevalence of H. pylori virulence factors has been reported, the aim of this work was to establish the H. pylori and vacA, cagA and babA2 gene status in 238 adult patients, from a marginal urban area of Mexico, with chronic gastritis. METHODS:H. pylori was identified in cultures of gastric biopsies by nested PCR. vacA and cagA genes were detected by multiplex PCR, whereas babA2 gene was identified by conventional PCR. RESULTS:H. pylori-positive biopsies were 143 (60.1%). All H. pylori strains were vacA+; 39.2% were cagA+; 13.3% were cagA+ babA2+ and 8.4% were babA2+. Mexican strains examined possessed the vacA s1, m1 (43.4%), s1, m2 (24.5%), s2, m1 (20.3%) and s2, m2 (11.9%) genotypes. CONCLUSION:These results show that the Mexican patients suffering chronic gastritis we have studied had a high incidence of infection by H. pylori. Forty four percent (63/143) of the H. pylori strains analyzed in this work may be considered as highly virulent since they possessed two or three of the virulence markers analyzed: vacA s1 cagA babA2 (9.8%, 14/143), vacA s1 babA2 (4.9%, 7/143), and vacA s1 cagA (29.4%, 42/143). However, a statistically significant correlation was not observed between vacAs1, cagA and babA2 virulence markers (chi2 test; P > 0.05).
journal_name
Ann Clin Microbiol Antimicrobjournal_title
Annals of clinical microbiology and antimicrobialsauthors
Paniagua GL,Monroy E,Rodríguez R,Arroniz S,Rodríguez C,Cortés JL,Camacho A,Negrete E,Vaca Sdoi
10.1186/1476-0711-8-14subject
Has Abstractpub_date
2009-04-30 00:00:00pages
14issn
1476-0711pii
1476-0711-8-14journal_volume
8pub_type
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章,多中心研究
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章,多中心研究
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-1-6
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-10-2
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journal_title:Annals of clinical microbiology and antimicrobials
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pub_type: 杂志文章
doi:10.1186/1476-0711-9-12
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-10-32
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-10-24
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journal_title:Annals of clinical microbiology and antimicrobials
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章,评审
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更新日期:2018-08-10 00:00:00
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/s12941-017-0222-0
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-3-13
更新日期:2004-07-15 00:00:00
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-6-3
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章,随机对照试验
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-13-1
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-6-17
更新日期:2007-12-28 00:00:00
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-11-31
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
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更新日期:2015-07-31 00:00:00