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Identification of multiple transcription start sites in the human insulin-like growth factor-I gene.

Abstract：

:We have localized four transcription initiation sites in the human insulin-like growth factor-I (IGF-I) gene. Two transcription start sites were identified which result in a longer and shorter version of the leader derived from the known exon 1 of the IGF-I gene. Transcription starting at the upstream transcription initiation site results in a leader exon 1 of about 1155 nucleotides (nt), whereas transcription starting at the downstream initiation site results in a leader of about 240 nt. The majority of the transcripts initiate at the latter site. We further identified a region in the human IGF-I gene between exons 1 and 2, which shows a high degree of homology with the rat IGF-I leader exon 1B. By means of the polymerase chain reaction (PCR) we detected human IGF-I mRNAs containing this novel leader. The corresponding exon was designated exon 1B according to the rat IGF-I gene terminology. PCR and RNase protection analyses identified two transcription start sites within this alternative leader exon 1B. Transcription initiated at the most upstream start site results in a leader of about 750 nt, whereas transcription starting at the downstream site is heterogeneous, resulting in leaders of 65-75 nt long. No consensus TATA-box or AT-rich regions are present immediately upstream of all four transcription start sites identified, nor are these regions particularly GC-rich. The IGF-I gene is known to be expressed differentially in a tissue- and development-specific fashion. Differential activation of multiple promoters could very well play a crucial role in IGF-I gene regulation.

journal_name

Mol Cell Endocrinol

journal_title

Molecular and cellular endocrinology

authors

Jansen E,Steenbergh PH,LeRoith D,Roberts CT Jr,Sussenbach JS

doi

10.1016/0303-7207(91)90192-u

subject

Has Abstract

pub_date

1991-06-01 00:00:00

pages

115-25

issue

1-2

eissn

0303-7207

issn

1872-8057

journal_volume

78

pub_type

杂志文章

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