Abstract:
:Multi-drug resistance (MDR) limits the effectiveness of chemotherapy. P-glycoprotein encoded by the MDR1 gene, is known to be implicated in MDR phenotype, but other factors could be determinant in MDR. The aim of this study was to investigate new molecular factors potentially associated with the MDR phenotype using a proteomic approach. Two dimensional fluorescence difference gel electrophoresis (2D-DIGE) and MALDI-TOF peptide mass fingerprinting were used to determine differentially expressed proteins between LoVo human colon carcinoma cell line and one of its MDR sublines (LoVo-R1). Thirty-four differentially expressed proteins were identified. They were classified into five groups based on their biological functions: i) proteins involved in energy request pathways, ii) in detoxification pathways, iii) in cell survival activity, iv) in drug transport and v) in chaperone functions. Among these proteins, endothelin 1 and proteasome subunit beta2 regulations were validated by immunofluorescence and Western blotting, respectively, showing complete consistency with 2D-DIGE results. In conclusion, the proteomic approach indicates that multiple mechanisms are simultaneously involved in MDR. These might be useful in the search for new forms of interventional therapeutic approaches for MDR reversal.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Visentin M,Simula MP,Sartor F,Petrucco A,De Re V,Toffoli Gsubject
Has Abstractpub_date
2009-05-01 00:00:00pages
1281-9issue
5eissn
1019-6439issn
1791-2423journal_volume
34pub_type
杂志文章abstract::In the present study, we report the utility of transrectal ultrasonography guidance for laparoscopic radical prostatectomy (LRP), and the effect of a novel surgical approach of 'seven key elements of operative skill for the early recovery of urinary continence' ('7 key elements'). Among 170 patients who underwent lapa...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2010.865
更新日期:2011-02-01 00:00:00
abstract::Although peritoneal lavage with distilled water performed after surgery prevents peritoneal seeding, cancer cells may avoid rupture under mild hypotonicity through regulatory volume decrease (RVD), which is the homeostatic regulation of ion and water transport. The aim of the present study was to investigate the effec...
journal_title:International journal of oncology
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abstract::Radioiodinated meta-iodobenzylguanidine (MIBG), an analogue of norepinephrine, has been used in management of neuroendocrine tumors. Recent studies reveal that distribution of radioiodinated MIBG in animals depends on the specific activity of this radiopharmaceutical. In order to clarify the effect of specific activit...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.4.815
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.11.5.1109
更新日期:1997-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2018-09-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
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abstract::Whether elderly patients with metastatic esophageal, gastroesophageal, and gastric cancer do as well with chemotherapy as their younger counterparts was investigated in this pooled analysis. In total, 367 patients from 8 consecutive, first-line trials were included: i) etoposide + cisplatin; ii) 5-fluorourucil + leuco...
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journal_title:International journal of oncology
pub_type: 杂志文章
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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doi:
更新日期:2006-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-08-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-05-01 00:00:00
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journal_title:International journal of oncology
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doi:
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journal_title:International journal of oncology
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doi:
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journal_title:International journal of oncology
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doi:
更新日期:2009-02-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2009-08-01 00:00:00
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journal_title:International journal of oncology
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doi:
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