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RNA interference in mammalia cells by RNA-3'-PNA chimeras.

Abstract：

:The discovery of siRNAs as the mediators of RNA interference has led to an increasing interest in their therapeutic applications. Chemical modifications are introduced into siRNAs to optimize the potency, the stability and the pharmacokinetic properties in vivo. Here, we synthesize and test the effects of RNA-3'-PNA chimeras on siRNA functioning and stability. We demonstrate that the chemical modifications are compatible with the siRNA machinery, because all the PNA-modified siRNAs can efficiently mediate specific gene silencing in mammalian cells. Furthermore, we find that the modification on the sense strand of siRNA results in an increased persistence of the activity, whereas modification on both strands results in enhanced nuclease resistance in serum.

journal_name

Int J Mol Sci

journal_title

International journal of molecular sciences

authors

Potenza N,Moggio L,Milano G,Salvatore V,Di Blasio B,Russo A,Messere A

doi

10.3390/ijms9030299

subject

Has Abstract

pub_date

2008-03-01 00:00:00

pages

299-315

issue

3

issn

1422-0067

journal_volume

9

pub_type

杂志文章

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