Mutational analysis of Smad7 in human cervical cancer.

Abstract:

:Our aim was to carry out mutational analysis of Smad7 exon 4 that codes for the Smad7 MH2 domain in human cervical cancer tissues. This study is warranted since genetic abnormalities of components of the transforming growth factor-beta (TGF-beta)/Smad signaling system have been implicated in a variety of human cancers. Further, Smad7 is an important member of the Smad family that functions as a negative feedback regulator of TGF-beta responses. By direct sequencing, we screened 60 histopathologically confirmed human cervical carcinomas for mutations in exon 4 of Smad7 that encodes the MH2 domain of the protein, a region of the protein believed to be critical for receptor interaction. No mutations or aberrations could be identified in any of the 60 analyzed tumor samples. However, we identified a previously reported, heterozygous, silent G to C variant in codon 391 of Smad7 that was found twice in the set of 60 clinical samples. Thirty of the clinical samples analyzed in the study were positive for the presence of high-risk human papillomavirus (HPV) subtypes, while the rest were oncogenic HPV-negative. The two instances of the silent variant was found in oncogenic HPV-negative samples. We report mutational analysis of Smad7 in cervical cancer for the first time. Mutations and variants of Smad7 are unlikely to be of major significance to the pathogenesis of HPV-induced cervical cancer.

journal_name

Oncol Rep

journal_title

Oncology reports

authors

Hariharan R,Babu JM,P R,Pillai MR

doi

10.3892/or_00000315

subject

Has Abstract

pub_date

2009-04-01 00:00:00

pages

1001-4

issue

4

eissn

1021-335X

issn

1791-2431

journal_volume

21

pub_type

杂志文章
  • BNIPL-2 promotes the invasion and metastasis of human hepatocellular carcinoma cells.

    abstract::Enhanced cell migration and invasion play key roles in cancer metastasis. However, the molecules involved in this process are not fully understood. In this study, a full-length human BNIPL-2 (Bcl-2/adenovirus E1B 19 kDa interacting protein 2 like-2) cDNA was transfected into human hepatocellular carcinoma cells with l...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Xie L,Qin W,Li J,He X,Zhang H,Yao G,Shu H,Yao M,Wan D,Gu J

    更新日期:2007-03-01 00:00:00

  • Specific role of JNK in the maintenance of the tumor-initiating capacity of A549 human non-small cell lung cancer cells.

    abstract::Deregulation of c-Jun NH2-terminal kinase (JNK) signaling is now increasingly reported in a variety of human malignancies. Non-small cell lung cancer (NSCLC) is among such human malignancies with aberrant JNK activation; yet the exact role(s) of JNK deregulation in NSCLC biology, in particular in vivo, remains unclear...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2655

    authors: Okada M,Shibuya K,Sato A,Seino S,Watanabe E,Suzuki S,Seino M,Kitanaka C

    更新日期:2013-10-01 00:00:00

  • Association of FOSB exon 4 unmethylation with poor prognosis in patients with late‑stage non‑small cell lung cancer.

    abstract::Alterations in DNA methylation have a central role in the development and outcome of most human malignancies. Non‑small cell lung cancer (NSCLC), the most common lung cancer, leads to the largest number of cancer‑related deaths worldwide. FBJ murine osteosarcoma viral oncogene homolog B (FOSB) is a key component of th...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.7431

    authors: Kim DS,Lee WK,Park JY

    更新日期:2020-02-01 00:00:00

  • Novel EXT1 mutation identified in a pedigree with hereditary multiple exostoses.

    abstract::Hereditary multiple exostoses (HME) is an autosomal dominant bone disorder characterized by the presence of multiple benign cartilage-capped tumors. EXT1 located on chromosome 8q23-q24 and EXT2 located on 11p11-p12 are the main disease-causing genes which are responsible for ~90% of HME cases. Mutations of EXT1 or EXT...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2891

    authors: Cao L,Liu F,Kong M,Fang Y,Gu H,Chen Y,Zhao C,Zhang S,Bi Q

    更新日期:2014-02-01 00:00:00

  • Quercetin induces gadd45 expression through a p53-independent pathway.

    abstract::Quercetin, a kind of flavonoid, is found in edible fruits and vegetables and has anti-tumorigenic activity. However, the mechanism of activity has not been elucidated. We show for the first time that gadd45 is a molecular target of quercetin, which inhibits growth of human cervical cancer HeLa cells. Apoptosis was det...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Yoshida T,Maeda A,Horinaka M,Shiraishi T,Nakata S,Wakada M,Yogosawa S,Sakai T

    更新日期:2005-11-01 00:00:00

  • Long non-coding RNA SNHG1 predicts a poor prognosis and promotes colon cancer tumorigenesis.

    abstract::Colon cancer is the main cause of cancer mortality worldwide. Its poor prognosis is mainly ascribed to high recurrence rates. Identifying novel prognostic biomarkers and therapeutic key points for management is crucial and important. Long non-coding RNAs (lncRNAs) are a class of RNAs, which have various roles in carci...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2018.6412

    authors: Yang H,Wang S,Kang YJ,Wang C,Xu Y,Zhang Y,Jiang Z

    更新日期:2018-07-01 00:00:00

  • Efficacy of oral UFT plus leucovorin therapy for colon cancer with ovarian and multiple liver metastases: report of two cases.

    abstract::Case 1: a patient was diagnosed as having ascending colon cancer with right ovarian metastasis, and underwent palliative right hemicolectomy plus oophorectomy. The tumor was a well-differentiated adenocarcinoma with right ovarian metastasis, and the disease was classified as stage IV. Oral chemotherapy with UFT plus L...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.8.5.1079

    authors: mukai M,Moriya H,Himeno S,Oida Y,mukohyama S,Nishi T,Nakasaki H,Satoh S,Makuuchi H

    更新日期:2001-09-01 00:00:00

  • Inhibition of proliferation, sprouting, tube formation and Tie2 signaling of lymphatic endothelial cells by the histone deacetylase inhibitor SAHA.

    abstract::Histone deacetylase (HDAC) inhibitors exert potent inhibitory effects on various types of human cancer. The pioneer drug suberoylanilide hydroxamic acid (SAHA) is currently used in the clinic for cancer treatment. However, the effect of SAHA on tumor lymphangiogenesis is unclear. We recently showed that SAHA suppresse...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2523

    authors: Cheng HT,Hung WC

    更新日期:2013-08-01 00:00:00

  • Possible involvement of TGF‑β‑SMAD‑mediated epithelial‑mesenchymal transition in pro‑metastatic property of PAX6.

    abstract::Paired box 6 (PAX6) is a transcription factor that has oncogenic features. In breast cancer, PAX6 facilitates tumor progression; however, the underlying mechanism is largely unknown. The majority of breast cancer‑related mortalities are associated with metastasis of cancer cells. Therefore, the present study aimed to ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2020.7644

    authors: Jin M,Gao D,Wang R,Sik A,Liu K

    更新日期:2020-08-01 00:00:00

  • Tunicamycin-induced ER stress regulates chemokine CCL5 expression and secretion via STAT3 followed by decreased transmigration of MCF-7 breast cancer cells.

    abstract::Chemokine C-C motif ligand 5 (CCL5) is an important marker related to the progression of breast cancer and is upregulated in cancer cells. However, the mechanism of the overexpression of CCL5 in tumours has not yet been clarified. The present study aimed to investigate the role of endoplasmic reticulum (ER) stress in ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2014.3479

    authors: Zhang Y,Liao S,Fan W,Wei W,Wang C,Sun S

    更新日期:2014-12-01 00:00:00

  • Triptolide induced DNA damage in A375.S2 human malignant melanoma cells is mediated via reduction of DNA repair genes.

    abstract::Numerous studies have demonstrated that triptolide induces cell cycle arrest and apoptosis in human cancer cell lines. However, triptolide-induced DNA damage and inhibition of DNA repair gene expression in human skin cancer cells has not previously been reported. We sought the effects of triptolide on DNA damage and a...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2012.2170

    authors: Chueh FS,Chen YL,Hsu SC,Yang JS,Hsueh SC,Ji BC,Lu HF,Chung JG

    更新日期:2013-02-01 00:00:00

  • An increase in the percentage of HLA-DR-positive peripheral leukocytes predicts a poor prognosis in patients with squamous cell carcinoma of the lung.

    abstract::Immunologic factors that predict survival in patients with lung cancer have not been established. We examined the relationship between the percentage of HLA-DR-positive peripheral leukocytes [HLA-DR+ (%)] and survival of patients with squamous cell carcinoma of the lung. Before initiating therapy, peripheral blood was...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Nakamura H,Idiris A,Kato Y,Kato H

    更新日期:2004-03-01 00:00:00

  • The effects of the Wnt/β‑catenin signaling pathway on apoptosis in HeLa cells induced by carbon ion irradiation.

    abstract::The molecular mechanisms underlying the biological effects of carbon ions are unclear. The aim of this study was to explore the Wnt/β‑catenin pathway in regulating carbon ion (12C6+) radiation‑induced cellular toxicity. HLY78 is a Wnt‑specific small molecular modulator, whose effects on 12C6+ radiation‑induced damage ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2020.7581

    authors: Si J,Zhang J,Gan L,Guo M,Xie Y,Di C,Sun C,Wang F,Yan J,Zhang H

    更新日期:2020-07-01 00:00:00

  • A targeting peptide improves adenovirus-mediated transduction of a glioblastoma cell line.

    abstract::The progress of the application of adenovirus in cancer gene therapy is hindered by the lack of expression of native adenovirus receptor on a variety of cancer types. Hence, strategies are needed to retarget the adenoviral vector to non-native cellular surface receptors. In the present study, a new peptide SWDIAWPPLKV...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2014.3065

    authors: Wang D,Li W,Zhang H,Mao Q,Xia H

    更新日期:2014-05-01 00:00:00

  • Latent membrane protein 1 mediates the resistance of nasopharyngeal carcinoma cells to TRAIL-induced apoptosis by activation of the PI3K/Akt signaling pathway.

    abstract::The 5-year survival rate of nasopharyngeal carcinoma (NPC) is still disappointing despite the much improved technologies in its treatment. Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) can selectively induce apoptosis in most tumor cells while sparing normal cells....

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2011.1423

    authors: Li SS,Yang S,Wang S,Yang XM,Tang QL,Wang SH

    更新日期:2011-12-01 00:00:00

  • Roles of the HGF/Met signaling in head and neck squamous cell carcinoma: Focus on tumor immunity (Review).

    abstract::c‑mesenchymal‑epithelial transition (Met) is a transmembrane tyrosine kinase receptor of hepatocyte growth factor (HGF). HGF/Met signaling stimulates numerous pathways, including the Ras/mitogenactivated protein kinase (MAPK), phosphatidylinositol 3‑kinase/protein kinase B and Wnt/β‑catenin pathways, which serve impor...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2020.7799

    authors: Liu D,Zhong M,Zhan D,Zhang Y,Liu S

    更新日期:2020-12-01 00:00:00

  • Mutational analysis of the PTEN/MMAC1 tumour suppressor gene in primary human malignant mesotheliomas.

    abstract::Eighteen primary human malignant mesotheliomas obtained from 18 patients were screened for point mutations and microdeletions/insertions in all exons of the tumour suppressor gene PTEN/MMAC1 by SSCP analysis. No mutation could be found. Our preliminary data indicate that disarrangements of PTEN/MMAC1 are at least not ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.8.6.1375

    authors: Papp T,Schipper H,Pemsel H,Unverricht M,Muller KM,Wiethege T,Schiffmann D,Rahman Q

    更新日期:2001-11-01 00:00:00

  • Latexin exhibits tumor-suppressor potential in pancreatic ductal adenocarcinoma.

    abstract::Recent studies suggest that latexin (Lxn) expression is involved in stem cell regulation and that it plays significant roles in tumor cell migration and invasion. The clinicopathological significance of Lxn expression and its possible correlation with CD133 expression in pancreatic ductal adenocarcinoma (PDAC) is curr...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4353

    authors: Xue Z,Zhou Y,Wang C,Zheng J,Zhang P,Zhou L,Wu L,Shan Y,Ye M,He Y,Cai Z

    更新日期:2016-01-01 00:00:00

  • Expression of CXCL12 and CXCR4 in pT3-stage gastric cancer does not correlate with peritoneal metastasis.

    abstract::CXCR4, a chemokine receptor, is considered to be involved in the metastastic formation of various types of cancer and could influence survival. More recently, CXCR4 was reported to be associated with peritoneal metastasis in gastric cancer, and CXCL12, its ligand, as a prognostic determinant among gastric cancer of va...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Tsuboi K,Kodera Y,Nakanishi H,Ito S,Mochizuki Y,Nakayama G,Koike M,Fujiwara M,Yamamura Y,Nakao A

    更新日期:2008-11-01 00:00:00

  • Rejection of adenovirus infection is independent of coxsackie and adenovirus receptor expression in cisplatin-resistant human lung cancer cells.

    abstract::The adenovirus vector-based cancer gene therapy is controversial. Low transduction efficacy is believed to be one of the main barriers for the decreased expression of coxsackie and adenovirus receptor (CAR) on tumor cells. However, the expression of CAR on primary tumor tissue and tumor tissue survived from treatment ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.4870

    authors: Zhang NH,Peng RQ,Ding Y,Zhang XS

    更新日期:2016-08-01 00:00:00

  • Immunolabelling of the inhibin/activin-βC subunit in normal and malignant human uterine cervical tissue and cervical cancer cell lines.

    abstract::Inhibins are dimeric glycoproteins, composed of an α-subunit and one of two possible β-subunits (βA or βB), with substantial roles in human reproduction and in endocrine-responsive tumours. Recently a novel β subunit named βC was described, although it is still unclear if normal or cancerous cervical epithelial cells ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Blankenstein T,Jückstock J,Shabani N,Kunze S,Brüning A,Bergauer F,Mylonas I

    更新日期:2011-01-01 00:00:00

  • Epigenetic changes of tumor suppressor genes, P15, P16, VHL and P53 in oral cancer.

    abstract::We performed methylation specific PCR to explore the mechanism of inactivation of tumor suppressor genes P15, P16, P53 and VHL in 48 oral SCC. The frequencies of aberrant methylation on the promoter of the P15, the P16, the P53 and the VHL genes were 0.27 (13/48), 0.42 (20/48), 0.04 (2/48) and none, respectively. Alto...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Yeh KT,Chang JG,Lin TH,Wang YF,Tien N,Chang JY,Chen JC,Shih MC

    更新日期:2003-05-01 00:00:00

  • Pancreatic tumor mass in a xenograft mouse model is decreased by treatment with therapeutic stem cells following introduction of therapeutic genes.

    abstract::Pancreatic cancer is the fourth most common cause of cancer-related mortality. In the present study, we employed 2 types of therapeutic stem cells expressing cytosine deaminase (CD) with or without human interferon-β (IFN‑β), HB1.F3.CD and HB1.F3.CD.IFN-β cells, respectively, to selectively treat pancreatic cancer. Th...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2564

    authors: Kim DJ,Yi BR,Lee HR,Kim SU,Choi KC

    更新日期:2013-09-01 00:00:00

  • Expression of the EP300, TP53 and BAX genes in colorectal cancer: Correlations with clinicopathological parameters and survival.

    abstract::E1A binding protein P300 (EP300), tumor protein P53 (TP53) and BCL2 associated X, apoptosis regulator (BAX) genes encode proteins which cooperate to regulate important cellular processes. The present study aimed to determine the expression levels of EP300, TP53 and BAX in colorectal cancer (CRC) and to investigate the...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.5687

    authors: Kowalczyk AE,Krazinski BE,Godlewski J,Kiewisz J,Kwiatkowski P,Sliwinska-Jewsiewicka A,Kiezun J,Sulik M,Kmiec Z

    更新日期:2017-07-01 00:00:00

  • Caudatin inhibits human hepatoma cell growth and metastasis through modulation of the Wnt/β-catenin pathway.

    abstract::In the present study, we investigated the antitumor activity of caudatin in the human hepatoma cell line SMMC‑7721 by analysis of cell viability, cell cycle distribution, apoptosis and metastasis. The results showed that caudatin impaired the cell viability and inhibited the growth of SMMC-7721 cells in a time- and do...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2749

    authors: Luo Y,Sun Z,Li Y,Liu L,Cai X,Li Z

    更新日期:2013-12-01 00:00:00

  • miR-216b inhibits glioma cell migration and invasion through suppression of FoxM1.

    abstract::MicroRNAs (miRNAs) play a vital role in tumour biological and pathologic processes. In the present study, we aimed to detect the expression and biological role of miR-216b in glioma. Our data showed that miR-216b was significantly downregulated in human glioma tissues and cells. Ectopic expression of miR-216b inhibite...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.5824

    authors: Zhang T,Ma G,Zhang Y,Huo H,Zhao Y

    更新日期:2017-09-01 00:00:00

  • MicroRNA-497 suppresses angiogenesis by targeting vascular endothelial growth factor A through the PI3K/AKT and MAPK/ERK pathways in ovarian cancer.

    abstract::MicroRNAs (miRNAs) have been shown to play an important role in diverse biological processes and cancer progression. The objective of the present study was to investigate the role of miR-497 in ovarian cancer angiogenesis. We found that miR-497 expression was downregulated in human ovarian cancer tissues, and the low ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2014.3439

    authors: Wang W,Ren F,Wu Q,Jiang D,Li H,Shi H

    更新日期:2014-11-01 00:00:00

  • Combined SN-38 and gefitinib treatment promotes CD44 degradation in head and neck squamous cell carcinoma cells.

    abstract::The aim of the present study was to search for an effective regimen among existing chemotherapies for head and neck squamous cell carcinoma (HNSCC). Among the tested drugs, we focused on combined SN-38, which is the active metabolite produced from irinotecan hydrochloride - a type I DNA topoisomerase inhibitor - after...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.6105

    authors: Nanbu T,Umemura N,Ohkoshi E,Nanbu K,Sakagami H,Shimada J

    更新日期:2018-01-01 00:00:00

  • STAT3 inhibition by WP1066 suppresses the growth and invasiveness of bladder cancer cells.

    abstract::Signal transducer and activator of transcription 3 (STAT3) regulates the expression of genes mediating cell survival, proliferation and angiogenesis and is aberrantly activated in various types of malignancies, including bladder cancer. We examined whether it could be a novel therapeutic target for bladder cancer usin...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.5902

    authors: Tsujita Y,Horiguchi A,Tasaki S,Isono M,Asano T,Ito K,Asano T,Mayumi Y,Kushibiki T

    更新日期:2017-10-01 00:00:00

  • Pien Tze Huang induces apoptosis in multidrug‑resistant U2OS/ADM cells via downregulation of Bcl‑2, survivin and P-gp and upregulation of Bax.

    abstract::Pien Tze Huang (PZH) is a well-known traditional Chinese formula that was first prescribed by a royal physician in the Ming Dynasty. PZH has been used to treat various types of cancers including osteosarcoma. Previous studies have shown that PZH may effectively inhibit osteosarcoma cell growth in vivo and in vitro via...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2916

    authors: Zhang Y,Wang Q,Niu S,Liu J,Zhang L

    更新日期:2014-02-01 00:00:00