The Apc 1322T mouse develops severe polyposis associated with submaximal nuclear beta-catenin expression.

Abstract:

BACKGROUND & AIMS:We previously demonstrated that the 2 APC mutations in human colorectal tumors are coselected, because tumorigenesis requires an optimal level of Wnt signaling. We and others subsequently showed that the truncated APC proteins in colorectal tumors usually retain a total of 1-2 beta-catenin binding/degradation repeats (20AARs); very few intestinal tumors have proteins with no 20AARs. The coselection of the "2 hits" at APC makes it difficult to undertake further mechanistic studies in this area in humans. In mice, however, second hits appear to vary with the strain or genetic background used. This suggested the possibility of creating suboptimal Apc genotypes in the mouse. METHODS:We have constructed a mouse, Apc(1322T), with a mutant protein retaining one 20AAR. After repeated backcrossing to the C57BL/6J background, we compared the 1322T animals with the widely used Min mouse in which the mutant Apc protein has zero 20AARs. RESULTS:In both mice, intestinal adenomas showed copy-neutral loss of heterozygosity, making them homozygous for the mutant Apc allele. 1322T animals had markedly more severe polyposis, with earlier-onset, larger, more numerous, and more severely dysplastic adenomas. 1322T tumors also had more marked Paneth cell differentiation and higher frequencies of crypt fission. Somewhat surprisingly, nuclear beta-catenin expression was lower in 1322T than Min tumors. CONCLUSIONS:We propose that the Apc(1322T) mutation produces submaximal beta-catenin levels that promote early tumor growth more effectively than the Apc(Min) mutation.

journal_name

Gastroenterology

journal_title

Gastroenterology

authors

Pollard P,Deheragoda M,Segditsas S,Lewis A,Rowan A,Howarth K,Willis L,Nye E,McCart A,Mandir N,Silver A,Goodlad R,Stamp G,Cockman M,East P,Spencer-Dene B,Poulsom R,Wright N,Tomlinson I

doi

10.1053/j.gastro.2009.02.058

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

2204-2213.e1-13

issue

7

eissn

0016-5085

issn

1528-0012

pii

S0016-5085(09)00342-4

journal_volume

136

pub_type

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