Abstract:
BACKGROUND & AIMS:We previously demonstrated that the 2 APC mutations in human colorectal tumors are coselected, because tumorigenesis requires an optimal level of Wnt signaling. We and others subsequently showed that the truncated APC proteins in colorectal tumors usually retain a total of 1-2 beta-catenin binding/degradation repeats (20AARs); very few intestinal tumors have proteins with no 20AARs. The coselection of the "2 hits" at APC makes it difficult to undertake further mechanistic studies in this area in humans. In mice, however, second hits appear to vary with the strain or genetic background used. This suggested the possibility of creating suboptimal Apc genotypes in the mouse. METHODS:We have constructed a mouse, Apc(1322T), with a mutant protein retaining one 20AAR. After repeated backcrossing to the C57BL/6J background, we compared the 1322T animals with the widely used Min mouse in which the mutant Apc protein has zero 20AARs. RESULTS:In both mice, intestinal adenomas showed copy-neutral loss of heterozygosity, making them homozygous for the mutant Apc allele. 1322T animals had markedly more severe polyposis, with earlier-onset, larger, more numerous, and more severely dysplastic adenomas. 1322T tumors also had more marked Paneth cell differentiation and higher frequencies of crypt fission. Somewhat surprisingly, nuclear beta-catenin expression was lower in 1322T than Min tumors. CONCLUSIONS:We propose that the Apc(1322T) mutation produces submaximal beta-catenin levels that promote early tumor growth more effectively than the Apc(Min) mutation.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Pollard P,Deheragoda M,Segditsas S,Lewis A,Rowan A,Howarth K,Willis L,Nye E,McCart A,Mandir N,Silver A,Goodlad R,Stamp G,Cockman M,East P,Spencer-Dene B,Poulsom R,Wright N,Tomlinson Idoi
10.1053/j.gastro.2009.02.058subject
Has Abstractpub_date
2009-06-01 00:00:00pages
2204-2213.e1-13issue
7eissn
0016-5085issn
1528-0012pii
S0016-5085(09)00342-4journal_volume
136pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract::In seven healthy volunteers pure pancreatic juice was obtained by endoscopic cannulation of the papilla of Vater. Synthetic secretin was intravenously infused in doses doubled every 20 min. The volume of pancreatic juice was proportional to the log of the secretin dose. A significant rise (P less than 0.05) in pancrea...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1976-04-01 00:00:00
abstract:BACKGROUND & AIMS:Refractory gastroesophageal reflux disease (GERD) reduces quality of life and creates significant financial burden on the health care system. Approximately 30% of patients with GERD who receive label-dose proton pump inhibitors (PPIs) still have symptoms. We performed a trial to evaluate the efficacy ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2020.02.031
更新日期:2020-06-01 00:00:00
abstract::Using a newly developed radioimmunoassay for porcine gastrin-releasing peptide in plasma, we studied the pharmacokinetics of this peptide after infusing it into pigs at two dose levels. The disappearance of the peptide from plasma was characterized by two components, a fast one (t 1/2 1.4 min) and a slow one (t 1/2 6....
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1984-08-01 00:00:00
abstract:BACKGROUND & AIMS:Sigmoidoscopy is an effective screening strategy for colorectal cancer that is not widely used by primary care providers. The aim of this study was to assess the impact of "academic detailing" in the form of an outreach educational seminar combined with implementation of on-site sigmoidoscopy services...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章
doi:10.1053/gast.1999.0029900304
更新日期:1999-08-01 00:00:00
abstract::Although long-term parenteral nutrition is lifesaving in patients with intestinal failure, it is expensive and associated with serious complications such as catheter sepsis, venous occlusions, and liver failure and severely impairs the quality of life in the short bowel patients. Therefore, treatments that increase th...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2005.09.068
更新日期:2006-02-01 00:00:00
abstract:BACKGROUND AND AIMS:CD4+CD25+Foxp3+ T-regulatory (Treg) cells control immune responses and maintain immune homeostasis. However, under inflammatory conditions, Treg cells produce cytokines that promote inflammation. We investigated production of tumor necrosis factor (TNF) by Treg cells in patients with acute hepatitis...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2017.11.277
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND & AIMS:Hepcidin is an antimicrobial peptide thought to be involved in the regulation of intestinal iron absorption. To further investigate its role in this process, we examined hepatic and duodenal gene expression in rats after the switch from a control diet to an iron-deficient diet. METHODS:Adult rats on ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2002.35353
更新日期:2002-09-01 00:00:00
abstract:BACKGROUND & AIMS:Long-term data on the risk of colorectal cancer according to dose, duration, and consistency of aspirin therapy are limited. METHODS:We conducted a prospective study of 47,363 male health professionals who were ages 40-75 years at enrollment in 1986. Biennially, we collected data on aspirin use, othe...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2007.09.035
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND & AIMS:New serologic markers of inflammatory bowel disease may be useful for differentiating between Crohn's disease and ulcerative colitis and for disease stratification. We profiled sugar-binding antibodies to identify novel antiglycan antibodies that may be associated with inflammatory bowel disease. MET...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2006.04.030
更新日期:2006-08-01 00:00:00
abstract:BACKGROUND & AIMS:Cyclooxygenase 2 (COX-2)-specific inhibitors (coxibs) decrease gastrointestinal (GI) events in controlled trials, but results in clinical practice are unknown. We assessed GI-related resource use and costs in patients switching from chronic nonsteroidal anti-inflammatory drug (NSAID) therapy to chroni...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(03)00900-4
更新日期:2003-08-01 00:00:00
abstract:BACKGROUND & AIMS:Although a number of epidemiologic studies have found dietary fiber and whole grains to be inversely associated with colorectal cancer incidence, studies of dietary and other risk factors for small intestinal cancer have been sparse and all of a case-control design. We conducted a prospective cohort s...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2008.07.015
更新日期:2008-10-01 00:00:00
abstract::The target appearance of grey scale ultrasound is thought to be a characteristic sign of gastrointestinal wall thickening. It consists of a strong echogenic center surrounded by a sonolucent rim. In a prospective controlled study, the sensitivity and specificity of the ultrasonic B-scan for Crohn's disease were invest...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1982-08-01 00:00:00
abstract:BACKGROUND & AIMS:Nitric oxide is a major inhibitory neurotransmitter in the enteric nervous system. Loss or dysfunction of nitrinergic neurons is associated with serious disruptions of motility, intractable symptoms, and long-term suffering. The aim of this study was to evaluate the effect of intrapyloric transplantat...
journal_title:Gastroenterology
pub_type: 评论,杂志文章
doi:10.1053/j.gastro.2005.08.055
更新日期:2005-12-01 00:00:00
abstract::A patient who developed obstructive jaundice as a complication of a liver biopsy is presented. The jaundice was found to be attributable to obstruction of the biliary tree at the level of the bifurcation by an intrahepatic hematoma. The patient had a complete and spontaneous recovery. ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract:DESCRIPTION:This expert review was commissioned and approved by the AGA Institute Clinical Practice Updates Committee and the AGA Governing Board to provide timely guidance on a topic of high clinical importance to the AGA membership. The intent is to evaluate the current data on mechanism of altered coagulation in pat...
journal_title:Gastroenterology
pub_type: 杂志文章,实务指引,评审
doi:10.1053/j.gastro.2019.03.070
更新日期:2019-07-01 00:00:00
abstract:BACKGROUND & AIMS:Intercellular communication through gap junctions has been proposed to be an important mechanism for the maintenance of tissue homeostasis. However, few studies have considered the role of gap junctions in gastric mucosa. The purpose of this study was to evaluate the distribution of gap junction-speci...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(95)90635-5
更新日期:1995-11-01 00:00:00
abstract:BACKGROUND & AIMS:Prior thermal stress induces heat shock protein 70 (HSP70) expression in the pancreas and protects against secretagogue-induced pancreatitis, but it is not clear that this thermal stress-induced protection is actually mediated by HSP70 since thermal stress may have other, non-HSP related, effects. ME...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2002.30314
更新日期:2002-01-01 00:00:00
abstract::Two types of benzodiazepine receptors have been identified in the central nervous system. The aim of these experiments was to determine if ligands for these receptors alter basal water absorption by rat ileum in vivo after central administration. Specifically, the effects on net water flux of the systemic and central ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(87)91023-7
更新日期:1987-08-01 00:00:00
abstract:BACKGROUND & AIMS:The early detection of colorectal cancer is desired because this cancer can be cured surgically if diagnosed early. The purpose of the present study was to determine the feasibility of a new methodology for isolating colonocytes from naturally evacuated feces, followed by cytology or molecular biology...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2005.10.007
更新日期:2005-12-01 00:00:00
abstract:BACKGROUND & AIMS:Ethanol impairs the mitochondrial transport of reduced glutathione (GSH), resulting in lower mitochondrial GSH (mGSH) levels. Our purpose was to evaluate the role of acetaldehyde on the regulation of mGSH in HepG2 cells. METHODS:mGSH levels and transport, mitochondrial membrane microviscosity, and li...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2003.50089
更新日期:2003-03-01 00:00:00
abstract:BACKGROUND/AIMS:Despite putative roles of calcium in biliary physiology and gallstone formation, quantitative aspects of calcium binding to bile salt (BS) monomers, simple micelles, mixed micelles, and vesicles, which constitute the lipid aggregates in bile, remain unexplored. METHODS:Calcium activity was measured usi...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(94)90134-1
更新日期:1994-09-01 00:00:00
abstract::Evidence-based medicine combines clinical expertise and the best available evidence from systematic research to aid decision making in patient care. Levels of evidence can be graded from I to V, with level I, the strongest, coming from large randomized controlled trials (RCTs). When a definitive RCT has not been perfo...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1016/s0016-5085(97)80022-4
更新日期:1997-12-01 00:00:00
abstract:BACKGROUND & AIMS:Germline mutations of DNA mismatch repair genes are responsible for cancer susceptibility in hereditary nonpolyposis colorectal cancer (HNPCC) kindreds. Transforming growth factor beta type II receptor (TGF-beta RII) has been found to be somatically altered in HNPCC. The aim of this study was to clari...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(97)70216-6
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND & AIMS:We have previously shown that patients with irritable bowel syndrome (IBS) have impaired transit of intestinal gas loads. Because abnormal gas retention can be experimentally reproduced in healthy subjects by pharmacological inhibition of gut motility, we hypothesized that impaired gas transit and ret...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1053/gast.2002.33658
更新日期:2002-06-01 00:00:00
abstract:BACKGROUND & AIMS:Crohn's disease (CD) is an inflammatory bowel disease characterized by uncontrolled immune responses to bacterial flora, with excessive activation of T lymphocytes. MICA is a stress-induced major histocompatibility complex-related molecule expressed on normal intestinal epithelial cells (IECs) and rec...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2007.03.025
更新日期:2007-06-01 00:00:00
abstract::We have prospectively studied changes in serum postprandial cholylglycine (CG) concentration during 297 pregnancies. We found an increase in CG concentration from 0.3 mumol/L at 15 weeks' pregnancy to 0.6 mumol/L at 40 weeks' pregnancy. Although this increase was statistically significant (p less than 0.005), median c...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(86)90682-7
更新日期:1986-10-01 00:00:00
abstract::The effect of somatostatin 28, a noval intestinal peptide containing somatostatin in its C-terminal portion, was tested on basal exocrine pancreatic secretion on conscious dogs and after stimulation by secretin (1 CU x kg-1 x hr-1), caerulein (37.5 ng x kg-1 x hr-1) or secretin (2 CU x kg-1 x hr-1) caerulein (37.5 ng ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1980-10-01 00:00:00
abstract:BACKGROUND & AIMS:Modulation of the host innate immune response is an attractive means of inhibiting hepatitis C virus (HCV) replication. Having previously determined that expression of the interferon-sensitive gene (ISG)15 protease USP18 is increased in the liver biopsy specimens of patients who do not respond to inte...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2006.08.043
更新日期:2006-11-01 00:00:00
abstract:BACKGROUND & AIMS:There is no clear and point for the response to treatment of gastrointestinal human cytomegalovirus (HCMV) disease. HCMV-DNA quantitation on gastrointestinal biopsy specimens has proven its value for the diagnosis of gastrointestinal HCMV disease in patients with acquired immunodeficiency syndrome (AI...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1996.v111.pm8690210
更新日期:1996-08-01 00:00:00
abstract:BACKGROUND:No enzyme replacement therapy exists for patients with congenital sucrase-isomaltase deficiency (CSID). A by-product of the manufacture of baker's yeast is a liquid preparation containing high sucrase activity. The aim of the present study was to investigate the activity and stability of this preparation and...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0016-5085(93)90950-h
更新日期:1993-10-01 00:00:00