Abstract：
:Analysis of autoantibodies (AAB) by indirect immunofluorescence (IIF) remains the hallmark of diagnosing autoimmune diseases despite the introduction of multiplex techniques. Non-organ specific AAB are screened in routine diagnostics by IIF on HEp-2 cells. However, IIF results vary due to objective (e.g., cell fixation) and subjective factors (e.g., expert knowledge). Therefore, inter- and intralaboratory variance is relatively high. Standardisation of AAB testing by IIF remains a critical issue in and between routine laboratories and may be improved by automated interpretation systems. An overview of existing interpretation techniques will be given taking into account own data of the first fully automated reading system AKLIDES. The novel system provides fully automated reading of IIF images and software algorithms for the mathematical description of IIF AAB patterns. It can be used for screening and preclassification of non-organ specific AAB in routine diagnostics regarding systemic autoimmune and autoimmune liver diseases. Furthermore, this system paves the way for economic data processing of cell-based IIF assays and can contribute to the reduction of interlaboratory variance of AAB testing. More sophisticated pattern recognition algorithms and novel calibration systems will improve standardised quantifications of IIF image interpretation.
journal_name
Autoimmun Revjournal_title
Autoimmunity reviewsauthors
Hiemann R,Büttner T,Krieger T,Roggenbuck D,Sack U,Conrad Kdoi
10.1016/j.autrev.2009.02.033subject
Has Abstractpub_date
2009-09-01 00:00:00pages
17-22issue
1eissn
1568-9972issn
1873-0183pii
S1568-9972(09)00073-1journal_volume
9pub_type
杂志文章,评审abstract：:There is a thriving interest in the field of hereditary autoinflammatory disorders (HAID), a gamut of heterogeneous conditions deriving from an aberrant orchestration of innate immunity, unified by the common feature of seemingly unprovoked inflammation, which might be systemic or occur in localized niches of the orga...
journal_title：Autoimmunity reviews
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journal_title：Autoimmunity reviews
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Autoimmunity reviews
pub_type： 杂志文章,评审
doi：10.1016/j.autrev.2010.12.001
更新日期：2011-04-01 00:00:00
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journal_title：Autoimmunity reviews
pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
doi：10.1016/j.autrev.2016.12.011
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pub_type： 杂志文章,评审
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更新日期：2007-03-01 00:00:00
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journal_title：Autoimmunity reviews
pub_type： 杂志文章,评审
doi：10.1016/j.autrev.2006.03.001
更新日期：2006-11-01 00:00:00
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pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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journal_title：Autoimmunity reviews
pub_type： 杂志文章,评审
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更新日期：2007-11-01 00:00:00
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pub_type： 杂志文章,meta分析
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abstract：:Immune thrombocytopenia (ITP) is a rare autoimmune disease due to an abnormal T cell response, notably supported by splenic T follicular helper cells, that stimulates the proliferation and differentiation of autoreactive B cells. The antiplatelet autoantibodies they produce facilitate platelet phagocytosis by macropha...
journal_title：Autoimmunity reviews
pub_type： 杂志文章,评审
doi：10.1016/j.autrev.2017.04.012
更新日期：2017-06-01 00:00:00
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