A candidate pheromone receptor and two odorant receptors of the hawkmoth Manduca sexta.

Abstract:

:In this study, we cloned and characterized three Manduca sexta odorant receptors (ORs). One receptor is a putative pheromone receptor expressed exclusively in a cell associated with male-specific type-I trichoid sensilla. We describe the results of real-time PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) experiments that show MsextaOR1 is expressed only in male antennae. In situ hybridization labels a single cell associated with type-1 trichoid sensilla, which houses two neurons that have been previously determined to respond to the major components of the pheromone blend. The second receptor, MsextaOR2, was discovered using degenerate primers designed to conserved motifs of a unique group ORs that share as much as 88% identity. Comparison of RT-PCR, qRT-PCR, and in situ hybridization results with those of ORs in the Drosophila melanogaster Or83b subfamily shows a strong sequence and expression pattern similarity. The third receptor, MsextaOR3, was found by 5'-end sequencing of a normalized and subtracted cDNA library from male M. sexta antennae. RT-PCR and qRT-PCR show that this receptor is expressed only in male and female antennae. These are the first ORs, including a putative pheromone receptor, to be described from M. sexta.

journal_name

Chem Senses

journal_title

Chemical senses

authors

Patch HM,Velarde RA,Walden KK,Robertson HM

doi

10.1093/chemse/bjp002

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

305-16

issue

4

eissn

0379-864X

issn

1464-3553

pii

bjp002

journal_volume

34

pub_type

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