Abstract:
:In this study, we cloned and characterized three Manduca sexta odorant receptors (ORs). One receptor is a putative pheromone receptor expressed exclusively in a cell associated with male-specific type-I trichoid sensilla. We describe the results of real-time PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) experiments that show MsextaOR1 is expressed only in male antennae. In situ hybridization labels a single cell associated with type-1 trichoid sensilla, which houses two neurons that have been previously determined to respond to the major components of the pheromone blend. The second receptor, MsextaOR2, was discovered using degenerate primers designed to conserved motifs of a unique group ORs that share as much as 88% identity. Comparison of RT-PCR, qRT-PCR, and in situ hybridization results with those of ORs in the Drosophila melanogaster Or83b subfamily shows a strong sequence and expression pattern similarity. The third receptor, MsextaOR3, was found by 5'-end sequencing of a normalized and subtracted cDNA library from male M. sexta antennae. RT-PCR and qRT-PCR show that this receptor is expressed only in male and female antennae. These are the first ORs, including a putative pheromone receptor, to be described from M. sexta.
journal_name
Chem Sensesjournal_title
Chemical sensesauthors
Patch HM,Velarde RA,Walden KK,Robertson HMdoi
10.1093/chemse/bjp002subject
Has Abstractpub_date
2009-05-01 00:00:00pages
305-16issue
4eissn
0379-864Xissn
1464-3553pii
bjp002journal_volume
34pub_type
杂志文章相关文献
CHEMICAL SENSES文献大全abstract::The sweet taste of sucrose acts as an analgesic, whereas the taste of a bitter substance decreases pain tolerance. The present experiment explores the analgesic effect of a complex taste and asks how adding cocoa, a substance often associated with sweet foods but that has a bitter taste, to a sucrose solution affects ...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjq016
更新日期:2010-05-01 00:00:00
abstract::Aiming to unravel interspecific differences in olfactory preferences, we performed comparative studies of odor valence in flies, mice, and humans. Our analysis suggests a model where flies and mice share similar olfactory preferences, but neither species share odor preferences with humans. This model contrasts with a ...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjy074
更新日期:2019-01-01 00:00:00
abstract::Since the discovery of adult neurogenesis, a major issue is the role of newborn neurons and the function-dependent regulation of adult neurogenesis. We decided to use an animal model with a relatively simple brain to address these questions. In the adult cricket brain as in mammals, new neurons are produced throughout...
journal_title:Chemical senses
pub_type: 杂志文章,评审
doi:10.1093/chemse/bjm010
更新日期:2007-05-01 00:00:00
abstract::The sense of smell deteriorates in normal aging, but the underling mechanisms are still elusive. Here we investigated age-related alterations in expression patterns of odorant receptor (OR) genes and functional properties of olfactory sensory neurons (OSNs)-2 critical factors that define the odor detection threshold i...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjp056
更新日期:2009-10-01 00:00:00
abstract::Odors are inherently ambiguous and therefore susceptible to redundant sensory as well as context information. The identification of an odor object relies largely on visual input. Thus far, it is unclear whether visual and olfactory stimuli are indeed integrated at an early perceptual stage and which role the congruenc...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjy018
更新日期:2018-05-23 00:00:00
abstract::Prior research has documented a modulating effect of taste on swallowing. We hypothesized that presentation of tastant stimuli would be a significant variable in swallowing-respiratory coordination, duration of oral bolus preparation, and submental muscle contraction. Twenty-three healthy females were presented with 1...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjl037
更新日期:2007-02-01 00:00:00
abstract::The experiments had 2 main goals: 1) to add to the sparse literature on how retronasal aromas interact with bitter tastes, and 2) to determine whether modulation of taste intensity by aroma depends on temporal contiguity, as one might expect if flavor interactions depend on cross-modal binding (similar to object perce...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjw059
更新日期:2016-09-01 00:00:00
abstract::Glutathione, a natural substance, acts on calcium receptors on the tongue and is known to enhance basic taste sensations. However, the effects of glutathione on brain activity associated with taste sensation on the tongue have not been determined under standardized taste delivery conditions. In this study, we investig...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjw066
更新日期:2016-09-01 00:00:00
abstract::Mosquito control is essential to protect humans from mosquito-borne diseases. The host recognition between mosquitoes and humans is achieved by the mosquito olfactory system. Antennal sensilla, which house olfactory receptor neurons, are responsible for detecting chemical cues from hosts. To deepen our understanding o...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjv089
更新日期:2016-06-01 00:00:00
abstract::In this issue of Chemical Senses, Baker et al. propose a provocative and intriguing explanation for a commonly observed phenomenon in moth chemocommunication. Sex pheromones in moths typically consist of mixtures of long-chain unsaturated compounds in specific ratios. These ratios are correspondingly detected by male ...
journal_title:Chemical senses
pub_type: 评论,杂志文章
doi:10.1093/chemse/bjs007
更新日期:2012-05-01 00:00:00
abstract::Recent evidence has suggested that factors related to sensory perception may explain excess weight. The objective of this study was to consider multiple aspects while investigating the phenomenon of obesity. One goal was to compare taste acuity (taste threshold and density of fungiform papillae) in both normal weight ...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjv072
更新日期:2016-02-01 00:00:00
abstract::Different odorant-binding proteins (OBPs) were isolated from total antennal homogenates of male and female Bombyx mori. Proteins were separated according to their isoelectric point by using preparative fast-flow isoelectrofocusing. Odorant-binding proteins were identified in immunoblots by antisera raised against the ...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/22.5.503
更新日期:1997-10-01 00:00:00
abstract::To assess the feasibility of using odors as a potential mechanism for treating sleep apnea, we set out to test the hypothesis that odorants delivered during sleep would modify respiratory patterns without inducing arousal or wake in healthy sleepers. We used 2 mildly trigeminal odorants: the pleasant lavender and unpl...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjp079
更新日期:2010-01-01 00:00:00
abstract::The terminal nerve, which innervates the nasal epithelia of most jawed vertebrates, is believed to release neuropeptides that modulate activity of sensory receptor neurons. The terminal nerve usually contains gonadotropin-releasing hormone as well as at least one other peptide that has not been characterized, but whic...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/28.4.339
更新日期:2003-05-01 00:00:00
abstract::Our olfactory sense plays an important role in eating behavior by modulating our food preferences and intake. However, hunger or satiety may also influence how we perceive odors. Albeit speculative, contradictory results found in the past may have resulted from confounding by type of meal that participants ate to indu...
journal_title:Chemical senses
pub_type: 杂志文章,随机对照试验
doi:10.1093/chemse/bjv068
更新日期:2016-02-01 00:00:00
abstract::In rodents, the vomeronasal system controls social and sexual behavior. However, several mechanistic aspects of sensory signaling in the vomeronasal organ remain unclear. Here, we investigate the biophysical basis of a recently proposed vomeronasal signal transduction component-a Ca(2+)-activated Cl(-) current. As the...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjw077
更新日期:2016-10-01 00:00:00
abstract::The present study analyzed 42 organic solvent extracts of scent mark pools from five dominant female common marmosets by gas chromatography (GC) and combined GC and mass spectrometry. We determined whether there were qualitative or quantitative differences between the chemical composition of scent marks from individua...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/26.5.449
更新日期:2001-06-01 00:00:00
abstract::Sodium taste transduction is thought to occur via an amiloride-sensitive, sodium-selective pathway and an amiloride-insensitive, cation nonselective, anion-dependent pathway(s). It has been shown by others that amiloride, an epithelial sodium channel (ENaC) blocker, significantly reduces the chorda tympani nerve respo...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/28.5.447
更新日期:2003-06-01 00:00:00
abstract::The adult olfactory system undergoes experience-dependent plasticity to adapt to the olfactory environment. This plasticity may be accompanied by perceptual changes, including improved olfactory discrimination. Here, we assessed experience-dependent changes in the perception of a homologous aldehyde pair by testing mi...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjv065
更新日期:2016-02-01 00:00:00
abstract::In order to assess the influence of odors on human performance and implicit memory for odors, 108 subjects completed a variety of tests in weakly scented (jasmine, lavender or odorless) rooms without having been made aware of the odor. After a 30 min interval the subjects were shown slides of different surroundings, i...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/24.3.317
更新日期:1999-06-01 00:00:00
abstract::Pheromone-sensitive sensilla trichodea of nine moth species belonging to six families and three superfamilies of Lepidoptera were immunolabelled with an antiserum against the pheromone-binding protein of Antheraea polyphemus. Strong immunolabelling of the sensillum lymph was observed in all long sensilla trichodea of ...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/21.6.719
更新日期:1996-12-01 00:00:00
abstract::Ten SW.B6 SOA nontaster strains congenic with the SWR/J SOA taster inbred strain were bred via repeated backcross-intercross cycles, with selection for nontasting in each cycle. Preference ratio distributions and phenotypic proportions across cycles at 0.1 mM SOA were consistent with monogenic predictions. The SW.B6 m...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/21.5.507
更新日期:1996-10-01 00:00:00
abstract::It is commonly agreed upon a strong link between emotion and olfaction. Odor-evoked memories are experienced as more emotional compared with verbal, visual, and tactile stimuli. Moreover, the emotional quality of odor cues increases memory performance, but contrary to this, odors are poor retrieval cues for verbal lab...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjv037
更新日期:2015-09-01 00:00:00
abstract::We have previously modeled the interaction of the sweet protein brazzein with the extracellular domains of the sweet taste receptor. Here, we describe the application of that model to the design of 12 new highly potent analogs of brazzein. Eight of the 12 analogs have higher sweetness potency than wild-type brazzein. ...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjp048
更新日期:2009-10-01 00:00:00
abstract::Extracellular neuronal responses were recorded from the posterior insular cortex following electrical and chemical stimulation of the thalamic reticular nucleus (Rt) regions. In the present study, most neurons (29/32) were first characterized for their responses to electrical stimulation of the superior laryngeal (SL)...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjg064
更新日期:2003-10-01 00:00:00
abstract::Feeding behavior of adult rainbow trout (Oncorhynchus mykiss) is released by visual and/or chemical stimuli. Detection of either a conditioned visual or a conditioned chemical stimulus creates an excitatory feeding state within the central nervous system which turns on feeding behavior composed of swimming, turning an...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/22.4.375
更新日期:1997-08-01 00:00:00
abstract::In order to study potential mixture interactions among bitter compounds, selected sodium salts were added to five compounds presented either alone or as binary bitter-compound mixtures. Each compound was tested at a concentration that elicited 'weak' perceived bitterness. The bitter compounds were mixed at these conce...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjh045
更新日期:2004-06-01 00:00:00
abstract::Phagocytic cells in the taste buds of rat circumvallate papillae after the sectioning of bilateral glossopharyngeal nerves were examined by electron microscopy and immunohistochemistry. Electron micrographs taken 1 day after denervation revealed that flat-shaped cells were present just beneath the taste buds and that ...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/21.4.467
更新日期:1996-08-01 00:00:00
abstract::Response accuracy and odor-sampling times were used to compare the ability of mice to detect (+)-carvone and (+)-terpinen-4-ol and to discriminate between enantiomers of carvone and of terpinen-4-ol. Except for increased odor sampling when mice were first exposed to the (+)-carvone odor, there was no difference in odo...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjm039
更新日期:2007-09-01 00:00:00
abstract::The central projections of olfactory receptor cells associated with two distinct types of antennal sensilla in the sphinx moth Manduca sexta were revealed by anterograde staining. In both sexes, receptor axons that arise from sexually isomorphic, type-II trichoid sensilla (and possibly some basiconic sensilla) project...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/20.3.313
更新日期:1995-06-01 00:00:00