Abstract:
:Initiation of DNA replication must be restricted to occur only once per cell cycle. In most bacteria, DnaA protein binds replication origins and promotes the initiation of DNA replication. We have found that in Bacillus subtilis, DnaA only colocalizes with origin regions at early or late stages of the cell cycle, when the replication machinery is assembling or disassembling, respectively. In contrast, DnaA colocalizes with the DNA replication machinery during most of the cell cycle. Indeed, we present evidence that a primary function of YabA, a negative regulator of replication initiation, is to tether DnaA to the polymerase-clamp protein DnaN. Thus, YabA ensures that once the origin is duplicated, it moves away from the replisome and from DnaA. We propose that DnaA colocalization with origins is specific to the time of initiation, and that replisome/YabA-mediated spatial sequestration of DnaA prevents inappropriate reinitiation of DNA replication.
journal_name
Dev Celljournal_title
Developmental cellauthors
Soufo CD,Soufo HJ,Noirot-Gros MF,Steindorf A,Noirot P,Graumann PLdoi
10.1016/j.devcel.2008.09.010subject
Has Abstractpub_date
2008-12-01 00:00:00pages
935-41issue
6eissn
1534-5807issn
1878-1551pii
S1534-5807(08)00392-4journal_volume
15pub_type
杂志文章abstract::The Golgi apparatus undergoes a ubiquitin-dependent disassembly and reassembly process during each cycle of cell division. Here we report the identification of the Golgi t-SNARE syntaxin 5 (Syn5) as the ubiquitinated substrate. Syn5 is monoubiquitinated by the ubiquitin ligase HACE1 in early mitosis and deubiquitinate...
journal_title:Developmental cell
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doi:10.1016/j.devcel.2016.06.001
更新日期:2016-07-11 00:00:00
abstract::Although the mechanisms underlying the spatial pattern formation of sensory maps have been extensively investigated, those triggering sensory map formation during development are largely unknown. Here we show that the birth of pups instructively and selectively regulates the initiation of barrel formation in the somat...
journal_title:Developmental cell
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doi:10.1016/j.devcel.2013.09.002
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abstract::DNA crosslinking agents are commonly used in cancer chemotherapy; however, responses of normal tissues to these agents have not been widely investigated. We reveal in mouse interfollicular epidermal, mammary and hair follicle epithelia that genotoxicity does not promote apoptosis but paradoxically induces hyperplasia ...
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abstract::Inflammatory pathways protect the body from infection and promote healing following injury. Recent reports demonstrate the surprising involvement of these pathways during hematopoietic stem cell emergence from the hemogenic endothelium in both zebrafish and mice. ...
journal_title:Developmental cell
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doi:10.1016/j.devcel.2014.11.026
更新日期:2014-12-08 00:00:00
abstract::Reporting in Developmental Cell, Schlaitz et al. (2013) show that endoplasmic reticulum (ER) membrane exclusion from the mitotic spindle is an active process requiring REEP membrane proteins. REEP protein depletion results in ER membrane retention on the spindle and chromosomes, leading to defects in chromosome segreg...
journal_title:Developmental cell
pub_type: 评论,杂志文章
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abstract::Recent studies have yielded a number of important insights into the mechanisms of hair follicle development and cycling and have highlighted the particularly important roles played by stem cells and Wnt signaling pathways. ...
journal_title:Developmental cell
pub_type: 杂志文章,评审
doi:10.1016/s1534-5807(01)00022-3
更新日期:2001-07-01 00:00:00
abstract::Sox2 expression marks neural and sensory primordia at various stages of development. A 50 kb genomic region of chicken Sox2 was isolated and scanned for enhancer activity utilizing embryo electroporation, resulting in identification of a battery of enhancers. Although Sox2 expression in the early embryonic CNS appears...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/s1534-5807(03)00088-1
更新日期:2003-04-01 00:00:00
abstract::How kinetochores regulate microtubule dynamics to ensure proper kinetochore-microtubule interactions is unknown. Here, we studied this during early mitosis in Saccharomyces cerevisiae. When a microtubule shrinks and its plus end reaches a kinetochore bound to its lateral surface, the microtubule end attempts to tether...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2011.09.006
更新日期:2011-11-15 00:00:00
abstract::Blood vessels form an important interface between the environment and the organism by carrying oxygen and nutrients to all cells and thus determining cellular metabolism. It is therefore not surprising that oxygen and metabolism influence the development of the vascular network. Here, we discuss recent insights regard...
journal_title:Developmental cell
pub_type: 杂志文章,评审
doi:10.1016/j.devcel.2009.01.003
更新日期:2009-02-01 00:00:00
abstract::Serrate (SE) is a key component in RNA metabolism. Little is known about whether and how it can regulate epigenetic silencing. Here, we report histone methyltransferases ATXR5 and ATXR6 (ATXR5/6) as novel partners of SE. ATXR5/6 deposit histone 3 lysine 27 monomethylation (H3K27me1) to promote heterochromatin formatio...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2018.05.023
更新日期:2018-06-18 00:00:00
abstract::Metazoans express three unfolded protein response transducers (IRE1, PERK, and ATF6) ubiquitously to cope with endoplasmic reticulum (ER) stress. ATF6 is an ER membrane-bound transcription factor activated by ER stress-induced proteolysis and has been duplicated in mammals. Here, we generated ATF6alpha- and ATF6beta-k...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2007.07.018
更新日期:2007-09-01 00:00:00
abstract::Caspase proteases execute apoptosis but also function in development. In this issue of Developmental Cell, Weaver et al. report that C. elegans CED-3 caspase promotes animal growth through PMK-1/p38 kinase cleavage, and at the expense of pathogen and stress immunity, revealing an unexpected homeostatic relationship be...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2020.04.006
更新日期:2020-05-04 00:00:00
abstract::In vertebrate embryos, retinoic acid (RA) synthesized in the mesoderm by Raldh2 emanates to the hindbrain neuroepithelium, where it induces anteroposterior (AP)-restricted Hox expression patterns and rhombomere segmentation. However, how appropriate spatiotemporal RA activity is generated in the hindbrain is poorly un...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2011.03.011
更新日期:2011-04-19 00:00:00
abstract::In this issue of Developmental Cell, Tang et al. (2018) and Li et al. (2018) combine genetic manipulation, mechanical perturbation, and live imaging to show how mechanical forces and local growth factors intersect to influence epithelial behavior and cell fate specification within the developing lung. ...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2018.01.015
更新日期:2018-02-05 00:00:00
abstract::Messenger RNAs (mRNAs) are synthesized in the cell nucleus and transported through nuclear pores to the cytoplasm for protein synthesis. Reporting in Nature Cell Biology, Mor et al. now track in living cells in real time the journey of single mRNA molecules as they transit from nucleus to cytoplasm. ...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2010.05.021
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abstract::Cytoplasmic dynein-1 is a minus-end-directed motor protein that transports cargo over long distances and organizes the intracellular microtubule (MT) network. How dynein motor activity is harnessed for these diverse functions remains unknown. Here, we have uncovered a mechanism for how processive dynein-dynactin compl...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2017.12.023
更新日期:2018-01-22 00:00:00
abstract::Epithelial tubes are found in many vital organs and require uniform and correct tube diameters for optimal function. Tube size depends on apical membrane growth and subapical cytoskeletal reorganization, but the cues that coordinate these events to ensure functional tube shape remain elusive. We find that epithelial t...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2005.07.012
更新日期:2005-09-01 00:00:00
abstract::Neutrophils, in response to a chemoattractant gradient, undergo dynamic F-actin remodeling, a process important for their directional migration or chemotaxis. However, signaling mechanisms for chemoattractants to regulate the process are incompletely understood. Here, we characterized chemoattractant-activated signali...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2011.10.023
更新日期:2011-12-13 00:00:00
abstract::The conserved Hedgehog signaling pathway is essential for embryonic development and tissue homeostasis and is tightly regulated. In this issue of Developmental Cell, Ma et al. (2016) demonstrate that sumoylation works in parallel with phosphorylation to stabilize Smoothened, antagonizing its ubiquitination and subsequ...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2016.11.001
更新日期:2016-11-21 00:00:00
abstract::All complex multicellular organisms must solve the problem of generating diverse and appropriately patterned cell types. Asymmetric division, in which a single mother cell gives rise to daughters with distinct identities, is instrumental in the generation of cellular diversity and higher-level patterns. In animal syst...
journal_title:Developmental cell
pub_type: 杂志文章,评审
doi:10.1016/j.devcel.2009.05.014
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journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2017.11.019
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journal_title:Developmental cell
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doi:10.1016/j.devcel.2010.05.001
更新日期:2010-05-18 00:00:00
abstract::The 6th European Meiosis Workshop, sponsored by EMBO and organized by Franz Klein of the University of Vienna, met in mid-September. More than 150 participants who have not discovered anything more interesting than sex gathered in a bucolic Alpine valley near the town of Obertraun, Austria, surrounded by granite massi...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/s1534-5807(03)00336-8
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abstract::Changes to cell shape and interaction drive animal tissue morphogenesis. Reporting now in Developmental Cell, Del Signore et al. (2018) describe active lengthening of cell-cell contacts by Arp2/3-based actin networks. Through cycles of contact lengthening and shortening, the developing Drosophila eye passes through va...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2018.02.003
更新日期:2018-02-26 00:00:00
abstract::During embryogenesis, melanoblasts proliferate and migrate ventrally through the developing dermis and epidermis as single cells. Targeted deletion of Rac1 in melanoblasts during embryogenesis causes defects in migration, cell-cycle progression, and cytokinesis. Rac1 null cells migrate markedly less efficiently, but s...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2011.07.008
更新日期:2011-10-18 00:00:00
abstract::SAG/RBX2/ROC2 protein is an essential RING component of SCF E3 ubiquitin ligase. The role of SAG during embryogenesis remains unknown. We report a critical role for SAG in controlling vascular and neural development by modulating RAS activity via promoting degradation of neurofibromatosis type 1 (NF1). Mice mutant for...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2011.09.014
更新日期:2011-12-13 00:00:00
abstract::Connecting phosphorylation events to kinases and phosphatases is key to understanding the molecular organization and signaling dynamics of networks. We have generated a validated set of transgenic RNA-interference reagents for knockdown and characterization of all protein kinases and phosphatases present during early ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2014.07.027
更新日期:2014-10-13 00:00:00
abstract::Mbl is a bacterial actin homolog that controls cell morphogenesis in Bacillus subtilis. A functional GFP-Mbl fusion protein was used to examine the behavior of the helical cables formed by Mbl protein in live B. subtilis cells. The cables undergo dynamic changes during cell cycle progression. They are stable but not r...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/s1534-5807(02)00403-3
更新日期:2003-01-01 00:00:00
abstract::Localization of the actin crosslinking protein, alpha-actinin, to the cleavage furrow has been previously reported. However, its functions during cytokinesis remain poorly understood. We have analyzed the functions of alpha-actinin during cytokinesis by a combination of molecular manipulations and imaging-based techni...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2007.08.003
更新日期:2007-10-01 00:00:00
abstract::Airway smooth muscle is best known for its role as an airway constrictor in diseases such as asthma. However, its function in lung development is debated. A prevalent model, supported by in vitro data, posits that airway smooth muscle promotes lung branching through peristalsis and pushing intraluminal fluid to branch...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2020.02.001
更新日期:2020-04-06 00:00:00