Cell-cycle-dependent spatial sequestration of the DnaA replication initiator protein in Bacillus subtilis.

Abstract:

:Initiation of DNA replication must be restricted to occur only once per cell cycle. In most bacteria, DnaA protein binds replication origins and promotes the initiation of DNA replication. We have found that in Bacillus subtilis, DnaA only colocalizes with origin regions at early or late stages of the cell cycle, when the replication machinery is assembling or disassembling, respectively. In contrast, DnaA colocalizes with the DNA replication machinery during most of the cell cycle. Indeed, we present evidence that a primary function of YabA, a negative regulator of replication initiation, is to tether DnaA to the polymerase-clamp protein DnaN. Thus, YabA ensures that once the origin is duplicated, it moves away from the replisome and from DnaA. We propose that DnaA colocalization with origins is specific to the time of initiation, and that replisome/YabA-mediated spatial sequestration of DnaA prevents inappropriate reinitiation of DNA replication.

journal_name

Dev Cell

journal_title

Developmental cell

authors

Soufo CD,Soufo HJ,Noirot-Gros MF,Steindorf A,Noirot P,Graumann PL

doi

10.1016/j.devcel.2008.09.010

subject

Has Abstract

pub_date

2008-12-01 00:00:00

pages

935-41

issue

6

eissn

1534-5807

issn

1878-1551

pii

S1534-5807(08)00392-4

journal_volume

15

pub_type

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