Abstract:
:Two isogenic sets of Yersinia pestis strains were generated, composed of wild-type strains 231 and I-1996, their non-polar pH 6(-) mutants with deletions in the psaA gene that codes for its structural subunit or the whole operon, as well as strains with restored ability for temperature- and pH-dependent synthesis of adhesion pili or constitutive production of pH 6 antigen. The mutants were generated by site-directed mutagenesis of the psa operon and subsequent complementation in trans. It was shown that the loss of synthesis or constitutive production of pH 6 antigen did not influence Y. pestis virulence or the average survival time of subcutaneously inoculated BALB/c naïve mice or animals immunized with this antigen.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Anisimov AP,Bakhteeva IV,Panfertsev EA,Svetoch TE,Kravchenko TB,Platonov ME,Titareva GM,Kombarova TI,Ivanov SA,Rakin AV,Amoako KK,Dentovskaya SVdoi
10.1099/jmm.0.005678-0subject
Has Abstractpub_date
2009-01-01 00:00:00pages
26-36issue
Pt 1eissn
0022-2615issn
1473-5644journal_volume
58pub_type
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