The subcutaneous inoculation of pH 6 antigen mutants of Yersinia pestis does not affect virulence and immune response in mice.

Abstract:

:Two isogenic sets of Yersinia pestis strains were generated, composed of wild-type strains 231 and I-1996, their non-polar pH 6(-) mutants with deletions in the psaA gene that codes for its structural subunit or the whole operon, as well as strains with restored ability for temperature- and pH-dependent synthesis of adhesion pili or constitutive production of pH 6 antigen. The mutants were generated by site-directed mutagenesis of the psa operon and subsequent complementation in trans. It was shown that the loss of synthesis or constitutive production of pH 6 antigen did not influence Y. pestis virulence or the average survival time of subcutaneously inoculated BALB/c naïve mice or animals immunized with this antigen.

journal_name

J Med Microbiol

authors

Anisimov AP,Bakhteeva IV,Panfertsev EA,Svetoch TE,Kravchenko TB,Platonov ME,Titareva GM,Kombarova TI,Ivanov SA,Rakin AV,Amoako KK,Dentovskaya SV

doi

10.1099/jmm.0.005678-0

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

26-36

issue

Pt 1

eissn

0022-2615

issn

1473-5644

journal_volume

58

pub_type

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