Modification of carbonic anhydrase II with acetaldehyde, the first metabolite of ethanol, leads to decreased enzyme activity.

abstract：BACKGROUND:Plant latex is the cytoplasm of highly specialized cells known as laticifers, and is thought to have a critical role in defense against herbivorous insects. Proteins abundantly accumulated in latex might therefore be involved in the defense system. RESULTS:We purified latex abundant protein a and b (LA-a an...

journal_title：BMC biochemistry

authors： Kitajima S,Kamei K,Taketani S,Yamaguchi M,Kawai F,Komatsu A,Inukai Y

更新日期：2010-01-28 00:00:00

abstract：BACKGROUND:The anticancer drug, 6-mercaptopurine (6MP) is subjected to metabolic clearance through xanthine oxidase (XOD) mediated hydroxylation, producing 6-thiouric acid (6TUA), which is excreted in urine. This reduces the effective amount of drug available for therapeutic efficacy. Co-administration of allopurinol, ...

journal_title：BMC biochemistry

authors： Kalra S,Jena G,Tikoo K,Mukhopadhyay AK

更新日期：2007-05-18 00:00:00

abstract：BACKGROUND:The expression of the drug-metabolizing enzyme cytochrome P450 3A4 (CYP3A4) is regulated by the pregnane x receptor (PXR), which is modulated by numerous signaling pathways, including the cyclin-dependent kinase (Cdk) pathway. Flavonoids, commonly consumed by humans as dietary constituents, have been shown t...

journal_title：BMC biochemistry

authors： Dong H,Lin W,Wu J,Chen T

更新日期：2010-06-16 00:00:00

abstract：BACKGROUND:We have previously described the identification and characterization of polyserase-1 and polyserase-2, two human serine proteases containing three different catalytic domains within the same polypeptide chain. Polyserase-1 shows a complex organization and it is synthesized as a membrane-bound protein which c...

journal_title：BMC biochemistry

authors： Cal S,Peinado JR,Llamazares M,Quesada V,Moncada-Pazos A,Garabaya C,López-Otín C

更新日期：2006-03-27 00:00:00

abstract：BACKGROUND:Mycobacterium tuberculosis DNA topoisomerase I is an attractive target for discovery of novel TB drugs that act by enhancing the accumulation of the topoisomerase-DNA cleavage product. It shares a common transesterification domain with other type IA DNA topoisomerases. There is, however, no homology between ...

journal_title：BMC biochemistry

authors： Annamalai T,Dani N,Cheng B,Tse-Dinh YC

更新日期：2009-06-11 00:00:00

abstract：BACKGROUND:Aberrant mitochondrial function, including excessive reactive oxygen species (ROS) production, has been implicated in the pathogenesis of human diseases. The use of mitochondrial inhibitors to ascertain the sites in the electron transport chain (ETC) resulting in altered ROS production can be an important to...

journal_title：BMC biochemistry

authors： Gusdon AM,Fernandez-Bueno GA,Wohlgemuth S,Fernandez J,Chen J,Mathews CE

更新日期：2015-09-10 00:00:00

abstract：BACKGROUND:Helical repeat motifs are common among regulatory subunits for type-1 and type-2A protein Ser/Thr phosphatases. Yeast Sit4 is a distinctive type-2A phosphatase that has dedicated regulatory subunits named Sit4-Associated Proteins (SAPS). These subunits are conserved, and three human SAPS-related proteins are...

journal_title：BMC biochemistry

authors： Guergnon J,Derewenda U,Edelson JR,Brautigan DL

更新日期：2009-10-16 00:00:00

abstract：BACKGROUND:The two myogenic regulatory factors Myf5 and MyoD are basic helix-loop-helix muscle transcription factors undergoing differential cell cycle dependent proteolysis in proliferating myoblasts. This regulated degradation results in the striking expression of these two factors at distinct phases of the cell cycl...

journal_title：BMC biochemistry

authors： Doucet C,Gutierrez GJ,Lindon C,Lorca T,Lledo G,Pinset C,Coux O

更新日期：2005-12-01 00:00:00

abstract：BACKGROUND:The serotonin type 3 receptor (5-HT3R) is a member of a superfamily of ligand gated ion channels. All members of this family share a large degree of sequence homology and presumably significant structural similarity. A large number of studies have explored the structure-function relationships of members of t...

journal_title：BMC biochemistry

authors： Venkataraman P,Venkatachalan SP,Joshi PR,Muthalagi M,Schulte MK

更新日期：2002-06-13 00:00:00

abstract：BACKGROUND:Some functions of 4.1R in non-erythroid cells are directly related with its distinct sub-cellular localisation during cell cycle phases. During mitosis, 4.1R is implicated in cell cycle progression and spindle pole formation, and co-localizes with NuMA1. However, during interphase 4.1R is located in the nucl...

journal_title：BMC biochemistry

authors： Treviño MA,Rodríguez-Rodríguez M,Correas I,Marcilla M,Albar JP,Rico M,Jiménez MA,Bruix M

更新日期：2010-01-28 00:00:00

abstract：BACKGROUND:The inorganic (Pi) phosphate transporter (PiT) family comprises known and putative Na(+)- or H(+)-dependent Pi-transporting proteins with representatives from all kingdoms. The mammalian members are placed in the outer cell membranes and suggested to supply cells with Pi to maintain house-keeping functions. ...

journal_title：BMC biochemistry

authors： Bøttger P,Pedersen L

更新日期：2011-05-17 00:00:00

abstract：BACKGROUND:Leptin is an adipocyte-derived hormone that acts via its hypothalamic receptor (LEPRb) to regulate energy balance. A downstream effect essential for the weight-regulatory action of leptin is the phosphorylation and activation of the latent transcription factor STAT3 by LEPRb-associated Janus kinases (JAKs). ...

journal_title：BMC biochemistry

authors： Knobelspies H,Zeidler J,Hekerman P,Bamberg-Lemper S,Becker W

更新日期：2010-01-08 00:00:00

abstract：BACKGROUND:A novel fluorescent cAMP analog (8-[Pharos-575]- adenosine-3', 5'-cyclic monophosphate) was characterized with respect to its spectral properties, its ability to bind to and activate three main isoenzymes of the cAMP-dependent protein kinase (PKA-Ialpha, PKA-IIalpha, PKA-IIbeta) in vitro, its stability towar...

journal_title：BMC biochemistry

authors： Moll D,Prinz A,Brendel CM,Berrera M,Guske K,Zaccolo M,Genieser HG,Herberg FW

更新日期：2008-06-25 00:00:00

abstract：BACKGROUND:The pyridoxal-5'-phosphate (PLP)-dependent or vitamin B6-dependent enzymes that catalyze manifold reactions in the metabolism of amino acids belong to no fewer than four evolutionarily independent protein families. The multiple evolutionary origin and the essential mechanistic role of PLP in these enzymes ar...

journal_title：BMC biochemistry

authors： Vacca RA,Giannattasio S,Capitani G,Marra E,Christen P

更新日期：2008-06-19 00:00:00

abstract：BACKGROUND:The re-replication inhibitor Geminin binds to several transcription factors including homeodomain proteins, and to members of the polycomb and the SWI/SNF complexes. RESULTS:Here we describe the TATA-binding protein-like factor-interacting protein (TIPT) isoform 2, as a strong binding partner of Geminin. TI...

journal_title：BMC biochemistry

authors： Pitulescu ME,Teichmann M,Luo L,Kessel M

更新日期：2009-06-10 00:00:00

abstract：BACKGROUND:The Gram-negative bacterium Haemophilus influenzae is a glutathione auxotroph and acquires the redox-active tripeptide by import. The dedicated glutathione transporter belongs to the ATP-binding cassette (ABC)-transporter superfamily and displays more than 60% overall sequence identity with the well-studied ...

journal_title：BMC biochemistry

authors： Vergauwen B,Van der Meeren R,Dansercoer A,Savvides SN

更新日期：2011-11-16 00:00:00

abstract：BACKGROUND:The α-isoform of the Type 1A Phosphoinositide 3-kinases (PI3Kα) has protein kinase activity as well as phosphoinositide lipid kinase activity. The best described substrate for its protein kinase activity is its regulatory subunit, p85α, which becomes phosphorylated on Serine 608. Phosphorylation of Serine 60...

journal_title：BMC biochemistry

authors： Layton MJ,Saad M,Church NL,Pearson RB,Mitchell CA,Phillips WA

更新日期：2012-12-27 00:00:00

abstract：BACKGROUND:General iron-sulfur cluster biosynthesis proceeds through assembly of a transient cluster on IscU followed by its transfer to a recipient apo-protein. The efficiency of the second step is increased by the presence of HscA and HscB, but the reason behind this is poorly understood. To shed light on the functio...

journal_title：BMC biochemistry

authors： Füzéry AK,Oh JJ,Ta DT,Vickery LE,Markley JL

更新日期：2011-01-26 00:00:00

abstract：BACKGROUND:Enzymatic allergens of storage mites that contaminate stored food products are poorly characterized. We describe biochemical and immunological properties of the native alpha-amylase allergen Aca s 4 from Acarus siro, a medically important storage mite. RESULTS:A. siro produced a high level of alpha-amylase ...

journal_title：BMC biochemistry

authors： Pytelková J,Lepšík M,Sanda M,Talacko P,Marešová L,Mareš M

更新日期：2012-01-31 00:00:00

abstract：BACKGROUND:Peptide-membrane interactions play a key role in the binding, partitioning and folding of membrane proteins, the activity of antimicrobial and fusion peptides, and a number of other processes. To gain a better understanding of the thermodynamics of such interactions, White and Wimley created an interfacial h...

journal_title：BMC biochemistry

authors： Aliste MP,Tieleman DP

更新日期：2005-12-20 00:00:00

abstract：BACKGROUND:Presenilin-1 (PS1) is a transmembrane protein first discovered because of its association with familial Alzheimer's disease. Mice with null mutations in PS1 die shortly after birth exhibiting multiple CNS and non-CNS abnormalities. One of the most prominent features in the brains of PS1-/- embryos is a vascu...

journal_title：BMC biochemistry

authors： De Gasperi R,Gama Sosa MA,Elder GA

更新日期：2012-12-21 00:00:00

abstract：BACKGROUND:ATP-binding cassette transporter A1 (ABCA1) mediates the lipidation of exchangeable apolipoproteins, the rate-limiting step in the formation of high density lipoproteins (HDL). We previously demonstrated that HDL oxidized ex vivo by peroxidase-generated tyrosyl radical (tyrosylated HDL, tyrHDL) increases the...

journal_title：BMC biochemistry

authors： Hossain MA,Ngeth S,Chan T,Oda MN,Francis GA

更新日期：2012-01-16 00:00:00

abstract：BACKGROUND:Minichromosome maintenance proteins (Mcm) 2, 3, 4, 5, 6 and 7 are related by sequence and form a variety of complexes that unwind DNA, including Mcm4/6/7. A Mcm4/6/7 trimer forms one half of the Mcm2-7 hexameric ring and can be thought of as the catalytic core of Mcm2-7, the replicative helicase in eukaryoti...

journal_title：BMC biochemistry

authors： Ma X,Stead BE,Rezvanpour A,Davey MJ

更新日期：2010-09-22 00:00:00

abstract：:A functional ubiquitin proteasome system is essential for all eukaryotic cells and therefore any alteration to its components has potential pathological consequences. Though the exact underlying mechanism is unclear, an age-related decrease in proteasome activity weakens cellular capacity to remove oxidatively modifie...

journal_title：BMC biochemistry

authors： Dahlmann B

更新日期：2007-11-22 00:00:00

abstract：BACKGROUND:A group of abundant proteins of ~30 kDa is synthesized in silkworm larval peripheral fat body (PPFB) tissues and transported into the open circulatory system (hemolymph) in a time-depended fashion to be eventually stored as granules in the pupal perivisceral fat body (PVFB) tissues for adult development duri...

journal_title：BMC biochemistry

authors： Pakkianathan BC,Singh NK,Krishnan M,König S

更新日期：2012-02-28 00:00:00

abstract：BACKGROUND:Trichomonas vaginalis, a flagellated protozoan, is the agent responsible for trichomoniasis, the most common nonviral sexually transmitted infection worldwide. A reported 200 million cases are documented each year with far more cases going unreported. However, T. vaginalis is disproportionality under studied...

journal_title：BMC biochemistry

authors： Dirkx M,Boyer MP,Pradhan P,Brittingham A,Wilson WA

更新日期：2014-06-28 00:00:00

abstract：BACKGROUND:Glutamic peptidases, from the MEROPS family G1, are a distinct group of peptidases characterized by a catalytic dyad consisting of a glutamate and a glutamine residue, optimal activity at acidic pH and insensitivity towards the microbial derived protease inhibitor, pepstatin. Previously, only glutamic peptid...

journal_title：BMC biochemistry

authors： Jensen K,Østergaard PR,Wilting R,Lassen SF

更新日期：2010-12-01 00:00:00

abstract：BACKGROUNDS:Streptococcus pneumoniae expresses three distinct sialidases, NanA, NanB, and NanC, that are believed to be key virulence factors and thus, potential important drug targets. We previously reported that the three enzymes release different products from sialosides, but could share a common catalytic mechanism...

journal_title：BMC biochemistry

authors： Hayre JK,Xu G,Borgianni L,Taylor GL,Andrew PW,Docquier JD,Oggioni MR

更新日期：2012-10-02 00:00:00

abstract：BACKGROUND:The 5'-triphosphorylated, 2'-5'-linked oligoadenylate polyribonucleotides (2-5As) are central to the interferon-induced antiviral 2-5A system. The 2-5As bind and activate the RNase L, an endoRNase degrading viral and cellular RNA leading to inhibition of viral replication. The 2-5A system is tightly controll...

journal_title：BMC biochemistry

authors： Poulsen JB,Kjær KH,Justesen J,Martensen PM

更新日期：2015-06-26 00:00:00

abstract：BACKGROUND:The biosynthesis pathway of Pyrroloquinoline quinone, a bacterial redox active cofactor for numerous alcohol and aldose dehydrogenases, is largely unknown, but it is proven that at least six genes in Klebsiella pneumoniae (PqqA-F) are required, all of which are located in the PQQ-operon. RESULTS:New structu...

journal_title：BMC biochemistry

authors： Puehringer S,Metlitzky M,Schwarzenbacher R

更新日期：2008-03-27 00:00:00