Abstract:
:To identify the repair dynamics involved in high linear energy transfer (LET) radiation-induced DNA damage, phospho-H2AX (gammaH2AX) foci formation was analyzed after cellular exposure to iron ions (Fe-ions, 500 MeV u(-1), 200 KeV microm(-1)). The foci located at DNA damage sites were visualized using immunocytochemical methods. Since H2AX is phosphorylated at sites of radiation-induced double strand breaks (DSB), gammaH2AX foci were used to detect or illuminate tracks formed by DSB after exposure to various doses of ionizing radiation. Additional DSB-recognition proteins such as ATM phospho-serine 1981, DNA-PKcs phospho-threonine 2609, NBS1 phospho-serine 343 and CHK2 phospho-threonine 68 all co-localized with gammaH2AX at high LET radiation induced DSB. In addition, Fe-ion induced foci remained for longer times than X-radiation induced foci. These findings suggest that Fe-ion induced damage is repaired more slowly than X-radiation induced damage, possibly because Fe-ion induced damage or lesions are more complex or extensive. Antibodies for all these phosphorylated DNA DSB recognition proteins appear to be very effective for the detection and localization of DSB.
journal_name
J Radiat Resjournal_title
Journal of radiation researchauthors
Takahashi A,Yamakawa N,Kirita T,Omori K,Ishioka N,Furusawa Y,Mori E,Ohnishi K,Ohnishi Tdoi
10.1269/jrr.08007subject
Has Abstractpub_date
2008-11-01 00:00:00pages
645-52issue
6eissn
0449-3060issn
1349-9157pii
JST.JSTAGE/jrr/08007journal_volume
49pub_type
杂志文章abstract::Modern treatment machines have shown small inter-unit variability regarding beam data. Recently, vendor-provided average beam data, such as the Representative Beam Data (RBD) of the TrueBeam (Varian Medical Systems, Palo Alto, CA, USA), has been used for modeling of the Eclipse (Varian Medical Systems) treatment plann...
journal_title:Journal of radiation research
pub_type: 杂志文章,多中心研究
doi:10.1093/jrr/rrz082
更新日期:2020-03-23 00:00:00
abstract::This study evaluated synthetic trehalose dicorynomycolate (S-TDCM), an immunomodulator, for its survival enhancing capacity and behavioral toxicity in B6D2F1 female mice. In survival experiments, mice were administered S-TDCM (25-400 micrograms/mouse i.p.) 20-24 hr before 5.6 Gy mixed-field fission-neutron irradiation...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1269/jrr.38.45
更新日期:1997-03-01 00:00:00
abstract::Perirectal hyaluronate gel injection (HGI) appears to be a promising technique for healthy tissue dose sparing in pelvic radiotherapy. In this analysis, we report our initial experience of HGI in gynecologic brachytherapy, focusing on its safety and effectiveness for dose reduction to the rectum. Between July 2013 and...
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abstract::The purpose of this multi-institutional Phase II trial study was to prospectively investigate the efficacy of the herbal medicine TJ-14 for acute radiation-induced enteritis (ARE). TJ-14 was administered orally as a first-line treatment for ARE. The primary end point was efficacy at 1 week. The secondary end points we...
journal_title:Journal of radiation research
pub_type: 杂志文章,多中心研究
doi:10.1093/jrr/rrz025
更新日期:2020-01-23 00:00:00
abstract::Immunohistochemical examinations were performed on rat pulmonary tumors induced by inhalation exposures to 239PuO2 aerosols, or by X-ray-irradiation to identify and compare cellular origins or, in turn, target cells at risk for radiation carcinogenesis. Both plutonium-induced and X-ray-induced pulmonary tumors appeare...
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doi:10.1269/jrr.43.301
更新日期:2002-09-01 00:00:00
abstract::In the last few years, hospital-based facilities for carbon-ion radiotherapy are being constructed and proposed in Europe and Asia. During the next few years, several new facilities will be opened for carbon-ion radiotherapy in the world. These facilities in operation or under construction are categorized in two types...
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更新日期:2010-01-01 00:00:00
abstract::miR-34a is transcriptionally induced by the tumor suppressor gene p53, which is often downregulated in non-small cell lung cancer (NSCLC). To address whether the downstream signal of miR-34a is sufficient to induce apoptosis and to alter cellular radiosensitivity, a chemical synthetic miR-34a mimic was delivered into ...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rrs136
更新日期:2013-07-01 00:00:00
abstract::The current study retrospectively analyzed the results of radiotherapy for clinically localized sebaceous carcinoma of the eyelid.We reviewed records of 83 patients with histologically confirmed sebaceous carcinoma who were treated radiotherapeutically between 1983 and 2015. Sixty-five patients (78%) were initially tr...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rrz046
更新日期:2019-10-23 00:00:00
abstract::Six Japanese patients in Hokkaido area were diagnosed as xeroderma pigmentosum (XP) at two University Hospital; one patient, a 29 years old female (XP2AS) at Asahikawa Medical College, and five patients, namely, a 53 years old male (XP1SA), a 45 years old female (XP2SA), a 46 years old female (XP3SA), a 47 years old m...
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pub_type: 杂志文章
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更新日期:1994-09-01 00:00:00
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journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rrw024
更新日期:2016-07-01 00:00:00
abstract::Liquid ionization chambers (LICs) are highly sensitive to dose irradiation and have small perturbations because of their liquid-filled sensitive volume. They require a sensitive volume much smaller than conventional air-filled chambers. However, it has been reported that the collection efficiency has dependencies on t...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rrx088
更新日期:2018-05-01 00:00:00
abstract::The yield of OH radical induced by ionizing radiation was estimated by an empirical model; a prescribed diffusion model for a spur of single size applying to neutral water. Two representative spur distances were introduced, one for an incident primary charged particle and one for a representative secondary electron, t...
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pub_type: 杂志文章
doi:10.1269/jrr.46.333
更新日期:2005-09-01 00:00:00
abstract::Single-photon emission computed tomography/computed tomography (SPECT/CT) demonstrates the precise location of the sentinel lymph nodes (SLNs) in patients with breast cancer. We evaluated the relationship between SLNs and postoperative tangential fields by using SPECT/CT images. Subjects included 72 patients with earl...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rrv035
更新日期:2015-09-01 00:00:00
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更新日期:2019-01-01 00:00:00
abstract::Differentiation-induction therapy is an attractive approach in leukemia treatment. It has been suggested that the accumulation of intracellular reactive oxygen species (ROS) is involved in megakaryocytic differentiation induced by phorbol 12-myristate 13-acetate (PMA) in the K562 leukemia cell line. Therefore, a ROS-i...
journal_title:Journal of radiation research
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doi:10.1093/jrr/rrs125
更新日期:2013-05-01 00:00:00
abstract::Microbeams have undergone a renaissance since their introduction and early use in the mid 60s. Recent advances in imaging, software and beam delivery have allowed rapid technological developments in microbeams for use in a range of experimental studies. The resurgence in the use of microbeams since the mid 90s has coi...
journal_title:Journal of radiation research
pub_type: 杂志文章,评审
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更新日期:2009-03-01 00:00:00
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journal_title:Journal of radiation research
pub_type: 临床试验,杂志文章
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更新日期:2005-06-01 00:00:00
abstract::Millimeter waves are used in various fields, and the risks of this wavelength range for human health must be carefully evaluated. In this study, we investigated the effects of millimeter waves on genotoxicity and heat shock protein expression in human corneal epithelial (HCE-T) and human lens epithelial (SRA01/04) cel...
journal_title:Journal of radiation research
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abstract::In Japan, the use of adjuvant radiotherapy after prostatectomy for prostate cancer has not increased compared with the use of salvage radiotherapy. We retrospectively evaluated the outcome of adjuvant radiotherapy together with prognostic factors of outcome in Japan. Between 2005 and 2007, a total of 87 patients were ...
journal_title:Journal of radiation research
pub_type: 杂志文章,多中心研究
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更新日期:2014-05-01 00:00:00
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journal_title:Journal of radiation research
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更新日期:2006-02-01 00:00:00
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journal_title:Journal of radiation research
pub_type: 杂志文章
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更新日期:2006-06-01 00:00:00
abstract:BACKGROUND:The aim of this study is to evaluate the cost-effectiveness of proton beam therapy with cochlear dose reduction compared with conventional X-ray radiotherapy for medulloblastoma in childhood. METHODS:We developed a Markov model to describe health states of 6-year-old children with medulloblastoma after trea...
journal_title:Journal of radiation research
pub_type: 杂志文章
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更新日期:2014-03-01 00:00:00
abstract::The Radiation Effects Research Foundation (RERF) continues to conduct a follow-up study initiated some years ago of cancer mortality and incidence among the survivors of the atomic bombing of Hiroshima and Nagasaki exposed in utero. Although only 18 incident cases of cancer were identified in the years 1950-1984 (of w...
journal_title:Journal of radiation research
pub_type: 杂志文章,评审
doi:10.1269/jrr.32.supplement_231
更新日期:1991-03-01 00:00:00
abstract::Male C3H/HeN mice were exposed to 252Cf radiation and mated with unirradiated C57BL/6N females. F1 mice were analyzed for germline mutation at the paternally derived C3H/HeN allele of a hypervariable minisatellite locus, Ms6hm. This locus exhibited a high frequency of length change mutation spontaneously, and the muta...
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更新日期:1996-09-01 00:00:00
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更新日期:2017-11-01 00:00:00
abstract::In the 1970s and 1980s, Planel et al. reported that the growth of paramecia was decreased by shielding them from background radiation. In the 1990s, Takizawa et al. found that mouse cells displayed a decreased growth rate under shielded conditions. The purpose of the present study was to confirm that growth is impaire...
journal_title:Journal of radiation research
pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
abstract::Numerous attempts have been made to estimate the distance at which 50% of the individuals exposed to the A-bombing of Hiroshima and Nagasaki died, ostensibly from their exposure to ionizing radiation. It is difficult to convert most of these distance estimates to a dose-related LD50, since radiation shielding was igno...
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更新日期:1991-03-01 00:00:00