Effects of intraocular irrigating solutions on the spreading of rabbit corneal endothelial cells on extracellular matrices.

Abstract:

:The effects of four commercially available irrigating solutions on the spreading of rabbit corneal endothelial cells on various extracellular matrices were studied. Cultured rabbit corneal endothelial cells, suspended in one of the following intraocular irrigating solutions, Opeguard MA, BSS, BSS Plus, lactated Ringer solution (Lactec) or physiological saline, were placed on uncoated tissue culture plates or on plates coated with extracellular matrices (fibronectin, laminin, collagen type I, or collagen type IV). The cell area was measured after 45 minutes' incubation. The cells spread on all of the extracellular matrices examined but not on the uncoated tissue culture plates. On the fibronectin or laminin matrix, the cell area was significantly greater with Opeguard MA or BSS Plus. On laminin and collagen type IV, the cell area was the greatest with Opeguard MA. On collagen type I, the cell area was significantly greater with Opeguard MA, BSS, or BSS Plus. These results demonstrated that the rabbit corneal endothelial cells responded to the extracellular matrices, and that Opeguard MA or BSS Plus provided more favorable conditions for the spreading of these cells. These results indicated that both Opeguard MA and BSS Plus might aid the spreading of corneal endothelial cells during wound-healing immediately after intraocular surgery.

journal_name

Jpn J Ophthalmol

authors

Nishida T,Otori T

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

61-7

issue

1

eissn

0021-5155

issn

1613-2246

journal_volume

35

pub_type

杂志文章
  • Fluorophotometry with a small animal adapter.

    abstract::The usefulness of the Fluorotron Master fluorophotometer fitted with a small animal adapter was evaluated with tree shrews. We also determined that the optimal measurement parameters for these animals, which are regarded as promising for experimental use in ophthalmic research, are: fluorescein-Na concentration, 0.5 x...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:

    authors: Ishiko S,Yoshida A,Kitaya N,Abiko T,Mori F,Kado M,Takahashi M,Saito K

    更新日期:1996-01-01 00:00:00

  • Pharmacological dissection of multifocal electroretinograms of rabbits with Pro347Leu rhodopsin mutation.

    abstract:PURPOSE:To determine whether photoreceptor degeneration in transgenic (Tg) rabbits carrying the Pro347Leu rhodopsin mutation alters the neural activity of the middle and inner retinal neurons. METHODS:Multifocal electroretinograms (mfERGs) were recorded from eight 12-week-old Tg rabbits both before and after intravitr...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1007/s10384-010-0842-9

    authors: Yokoyama D,Machida S,Kondo M,Terasaki H,Nishimura T,Kurosaka D

    更新日期:2010-09-01 00:00:00

  • Use of infrared TV cameras built into head-mounted display to measure torsional eye movements.

    abstract:PURPOSE:The head-mounted display (HMD) has produced conflict between visual and vestibular stimuli because the HMD image does not move with the head motion of the wearer. The HMD can show binocular parallax three-dimensional (3D) images, in which vergence and accommodation conflict. Thus, the HMD may affect the normal ...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1016/s0021-5155(00)00289-6

    authors: Ukai K,Saida S,Ishikawa N

    更新日期:2001-01-01 00:00:00

  • Current practice in the management of branch retinal vein occlusion in Japan: Survey results of retina specialists in Japan.

    abstract:PURPOSE:To elucidate the current clinical practice patterns of branch retinal vein occlusion (BRVO) management by retina specialists in Japan in the era of anti-vascular endothelial growth factor (VEGF) therapy. STUDY DESIGN:A voting survey using an answer pad system. METHODS:On May 28, 2017, forty-one retina special...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章,多中心研究

    doi:10.1007/s10384-019-00685-4

    authors: Ogura Y,Kondo M,Kadonosono K,Shimura M,Kamei M,Tsujikawa A

    更新日期:2019-09-01 00:00:00

  • Long-term outcome of systemic cyclosporine treatment following penetrating keratoplasty.

    abstract:PURPOSE:To perform a retrospective study to evaluate the long-term outcome of systemic cyclosporine treatment as an adjunct to topical corticosteroid treatment after penetrating keratoplasty (PKP). METHODS:Twenty-six high-risk patients (27 eyes) who received systemic cyclosporine following PKP for an average of 5.4 mo...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1016/s0021-5155(01)00339-2

    authors: Inoue K,Kimura C,Amano S,Sato T,Fujita N,Kagaya F,Kaji Y,Tsuru T,Araie M

    更新日期:2001-07-01 00:00:00

  • Shortening the duration of prone positioning after macular hole surgery- comparison between 1-week and 1-day prone positioning.

    abstract:PURPOSE:To shorten the duration of prone positioning after macular hole surgery from 1 week to 1 day, and to evaluate preoperative factors and the initial hole closure rate. METHODS:The subjects were 33 patients (34 eyes) who underwent macular hole surgery between April 1998 and August 1999, and maintained the prone p...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1016/s0021-5155(01)00468-3

    authors: Isomae T,Sato Y,Shimada H

    更新日期:2002-01-01 00:00:00

  • Imaging of a retinal pigment epithelium aperture using polarization-sensitive optical coherence tomography.

    abstract:PURPOSE:To evaluate cases with a retinal pigment epithelium (RPE) aperture using polarization-sensitive optical coherence tomography (PS-OCT). STUDY DESIGN:Retrospective consecutive case series. METHODS:A retrospective study that included three eyes (three patients) with RPE aperture and age-related macular degenerat...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1007/s10384-020-00787-4

    authors: Obata R,Yoshinaga A,Yamamoto M,Komatsu K,Aoki N,Yamanari M,Sugiyama S,Minami T,Azuma K,Inoue T,Aihara M,Kato S

    更新日期:2020-11-10 00:00:00

  • Sweet's syndrome with panuveitis resembling Behçet's disease.

    abstract:PURPOSE:Sweet's syndrome (SS) is a skin disorder clinically characterized by fever, neutrophilia, and painful edematous plaques that occasionally causes posterior uveitis. We present two cases of SS with panuveitis resembling Behçet's disease (BD). SUBJECTS:Two patients with panuveitis associated with SS. OBSERVATION...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1007/s10384-012-0129-4

    authors: Matsumiya W,Kusuhara S,Yamada Y,Azumi A,Negi A

    更新日期:2012-05-01 00:00:00

  • Evaluation of lamina cribrosa thickness and depth in ocular hypertension.

    abstract:PURPOSE:To investigate the differences in thickness and depth of the lamina cribrosa (LC) between ocular hypertension (OH) patients and normal control subjects, using enhanced depth imaging (EDI) spectral-domain optical coherence tomography (SD-OCT). METHODS:EDI-OCT data were obtained from the subjects in a cross-sect...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1007/s10384-015-0407-z

    authors: Han JC,Choi DY,Kwun YK,Suh W,Kee C

    更新日期:2016-01-01 00:00:00

  • Posterior subtenon infusion of triamcinolone acetonide as adjunctive treatment to panretinal photocoagulation using pattern scan laser for diabetic retinopathy.

    abstract:PURPOSE:To estimate the effect of sub-Tenon's capsule triamcinolone acetonide injection (STTA) combined with panretinal photocoagulation (PRP) using pattern scan laser (PSL) for high risk non-proliferative diabetic retinopathy (NPDR), in terms of the inflammation and the progression of diabetic macular edema (DME). ST...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1007/s10384-018-0628-z

    authors: Yamada Y,Takamura Y,Matsumura T,Morioka M,Gozawa M,Inatani M

    更新日期:2018-11-01 00:00:00

  • The results of serial dynamic enhanced computed tomography in patients with carotid-cavernous sinus fistulas.

    abstract:PURPOSE:To assess serial dynamic enhanced computed tomography (serial DE-CT) as a diagnostic tool for carotid-cavernous sinus fistula (CCF). METHODS:Serial DE-CT was performed in seven patients (ages 31-74) with CCF. Contrast material was injected intravenously at a dose of 60 mL with an injection speed of 4 mL per se...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1016/s0021-5155(99)00118-5

    authors: Ohtsuka K,Hashimoto M

    更新日期:1999-11-01 00:00:00

  • Falciform retinal fold as sign of familial exudative vitreoretinopathy.

    abstract::We studied family members of 9 patients with falciform retinal fold, and found a number of cases showing features of familial exudative vitreoretinopathy (FEVR) in the fundi. Retinal fold was also seen in the eye of a cousin of the propositus. Three cases with falciform retinal fold were bilateral and 7 cases were uni...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:

    authors: Nishimura M,Yamana T,Sugino M,Kohno T,Yamana Y,Minei M,Sanui H

    更新日期:1983-01-01 00:00:00

  • Prevalence of superior segmental optic nerve hypoplasia in Korea.

    abstract:PURPOSE:To investigate the prevalence of superior segmental optic nerve hypoplasia in Korea. METHODS:We reviewed the fundus photographs of 3905 individuals who were 40 years old or older and who participated in the comprehensive medical examination program at the Health Promotion Center of Seoul National University Bu...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1007/s10384-009-0663-x

    authors: Han SB,Park KH,Kim DM,Kim TW

    更新日期:2009-05-01 00:00:00

  • Effects of voxel size on detection of lateral geniculate nucleus activation in functional magnetic resonance imaging.

    abstract:PURPOSE:To study the effect of voxel size on the detection of lateral geniculate nucleus (LGN) activation during visual stimulation using functional magnetic resonance imaging (fMRI) at 1.5 T. METHODS:Ten normal subjects were scanned during visual stimulation with flashing goggles, four times, with four different voxe...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1007/s10384-004-0124-5

    authors: Miki A,Liu CS,Liu GT

    更新日期:2004-11-01 00:00:00

  • Prevention of murine experimental corneal trauma by epigenetic events regulating claudin 6 and claudin 9.

    abstract:PURPOSE:To study the effects of epigenetic events on corneal barrier functions, we examined tight junctions (TJs), the most important factor in the barrier function of the cornea, by using in vitro and in vivo corneal trauma models. METHODS:We examined alteration of TJ-associated gene expression and corneal barrier fu...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1007/s10384-008-0524-z

    authors: Nishikiori N,Sawada N,Ohguro H

    更新日期:2008-05-01 00:00:00

  • Temporal raphe of the retinal nerve fiber layer revealed by medullated fibers.

    abstract::Three cases of medullated nerve fibers of the retina were seen. They had medullated nerve fibers in both the upper and lower quadrants of the temporal retina, whereby the temporal raphe of the retinal nerve fiber layer could be visualized. Some nerve fibers from the upper and lower quadrants crossed each other on the ...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:

    authors: Sakai T,Sano K,Tsuzuki K,Ueno M,Kawamura Y

    更新日期:1987-01-01 00:00:00

  • Pharmacokinetics of topically applied ciprofloxacin in rabbit tears.

    abstract::Fluoroquinolones provide an important single antibiotic therapy for bacterial keratitis caused by Staphylococcus aureus and numerous gram-negative bacteria, including Pseudomonas aeruginosa. The pharmacokinetics of three ocular fluoroquinolones, norfloxacin (CHIBOXIN, Merck, Sharp & Dohme), ciprofloxacin (CILOXAN, Alc...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:

    authors: Green LC,Callegan MC,Engel LS,Shimomura Y,Jasheway DW,O'Callaghan RJ,Hill JM

    更新日期:1996-01-01 00:00:00

  • Light-evoked changes of glycine-like immunoreactivity in the rat retina.

    abstract::Glycine is an important inhibitory neurotransmitter in the vertebrate retina. Although many reports of glycine localization by the immunocytochemical method have been published, none has distinguished the neurotransmitter function of glycine from metabolic change. If glycine is the neurotransmitter, it seems that it m...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:

    authors: Nishikawa S,Ishiguro S,Tamai M

    更新日期:1996-01-01 00:00:00

  • National survey of pellucid marginal corneal degeneration in Japan.

    abstract:PURPOSE:A national survey was conducted in Japan to analyze the clinical characteristics of pellucid marginal corneal degeneration (PMD). METHODS:A questionnaire was sent to all members of the Japan Cornea Society requesting demographic and clinical findings on PMD patients who had been examined between 2008 and 2010....

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章,多中心研究

    doi:10.1007/s10384-016-0462-0

    authors: Shimazaki J,Maeda N,Hieda O,Ohashi Y,Murakami A,Nishida K,Tsubota K,Japan Pellucid Marginal Corneal Degeneration Study Group.

    更新日期:2016-09-01 00:00:00

  • Measurement repeatability of the dynamic Scheimpflug analyzer.

    abstract:PURPOSE:To evaluate the repeatability of corneal deformation parameters measured using a dynamic Scheimpflug analyzer and the impact of baseline clinical factors on the repeatability of each parameter. STUDY DESIGN:Retrospective, cross-sectional study. METHODS:Forty-eight eyes (48 healthy subjects; mean age, 49.0 ± 1...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1007/s10384-017-0534-9

    authors: Miki A,Maeda N,Asai T,Ikuno Y,Nishida K

    更新日期:2017-11-01 00:00:00

  • Acute unilateral inner retinal dysfunction with photophobia: importance of electrodiagnosis.

    abstract:PURPOSE:To establish with negative electroretinogram (ERG) the clinical entity of eight patients with unilateral severe photophobia, essentially normal fundus, good visual acuity, and severe cone and rod dysfunction. STUDY DESIGN:Multicenter retrospective observation case series. METHODS:Comprehensive ophthalmologic ...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1007/s10384-020-00780-x

    authors: Hirakata T,Fujinami K,Saito W,Kanda A,Hirakata A,Ishida S,Murakami A,Tsunoda K,Miyake Y

    更新日期:2020-11-12 00:00:00

  • Spectral sensitivity of monochromatic ERG c-wave of chicken under color adaptation.

    abstract::The spectral sensitivity of the ERG c-wave was studied in the chicken under yellow or red light adaptation of various intensities, ie, 1, 3, 4 and 5 W/m2 at the corneal surface in the former, and 3, 4 and 5 W/m2 in the latter. The peak wavelength of the spectral sensitivity curve of the c-wave amplitude under both yel...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:

    authors: Takahashi K,Fukuda A,Mori T,Takahashi Y,Onoe S,Yoshimura Y,Tazawa Y

    更新日期:1993-01-01 00:00:00

  • Unilateral disc-shaped cataract with coloboma of lens.

    abstract::A case of unilateral disc-shaped cataract with coloboma of the lens is reported. The patient, a 17-year-old girl, had best corrected visual acuity of 0.01 (+ 9 D) in the right eye and 1.2 in the left eye. Exotropia of 10 Hirschberg degrees was found in her right eye. The visual disturbance of that eye was thought to b...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:

    authors: Kihara Y,Shimomura Y,Omoto T,Fukuda M

    更新日期:1988-01-01 00:00:00

  • Ocular findings in a patient with Prader-Willi syndrome.

    abstract::A 25-year-old woman is described whose clinical features included infantile hypotonia, obesity after infancy, intellectual impairment, dysmorphic facial features, short stature, small hands and feet, and abnormal dentition, which are typical of the Prader-Willi syndrome. The patient had almond-shaped eyes, spot-like h...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:

    authors: Wang XC,Norose K,Kiyosawa K,Segawa K

    更新日期:1995-01-01 00:00:00

  • Cycloplegic effect of 0.5% tropicamide and 0.5% phenylephrine mixed eye drops: objective assessment in Japanese schoolchildren with myopia.

    abstract:PURPOSE:To evaluate the cycloplegic effect of mixed eye drops containing 0.5% tropicamide and 0.5% phenylephrine in myopic children, and to determine whether their efficacy was associated with their clinical characteristics. METHODS:Eighty-one myopic children (age, mean +/- SD, 11.0 +/- 1.5 years; mean spherical equiv...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1007/s10384-006-0400-7

    authors: Hamasaki I,Hasebe S,Kimura S,Miyata M,Ohtsuki H

    更新日期:2007-03-01 00:00:00

  • Effect of 3,3'-iminodipropionitrile on experimental intravitreal proliferation in albino rabbits.

    abstract::The effect of 3,3'-iminodipropionitrile (IDPN) on experimental intravitreal proliferation in albino rabbits was studied by light and electron microscopy. About 2.5 x 10(5) tissue cultured fibroblasts were injected into the vitreous cavity of albino rabbits. Immediately after the injection, phosphate-buffered solution ...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:

    authors: Akiyama K

    更新日期:1990-01-01 00:00:00

  • Evaluation of efficacy of infliximab for retinal vasculitis and extraocular symptoms in Behçet disease.

    abstract:PURPOSE:We evaluated ocular symptoms and activity of retinal vasculitis of Behçet disease before and after infliximab therapy, using Behçet disease ocular attack score 24 (BOS24) and fluorescein angiography (FA) score. We also analyzed the efficacy of infliximab for ocular and extraocular symptoms. STUDY DESIGN:Retros...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1007/s10384-018-0589-2

    authors: Umazume A,Kezuka T,Usui Y,Suzuki J,Goto H

    更新日期:2018-05-01 00:00:00

  • Effects of a new excitotoxic amino acid, dysiherbaine, on cultured Müller cells.

    abstract:PURPOSE:To determine the pharmacological response when dysiherbaine acts on cultured Müller cells, considering the glutamate receptor functions. Dysiherbaine is a new excitotoxic amino acid, which was recently isolated from the liquid extract of a certain sponge. METHODS:Retinas of adult rabbits were used to culture t...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1016/s0021-5155(01)00488-9

    authors: Minei R

    更新日期:2002-03-01 00:00:00

  • Transconjunctival immunotherapy using cholera toxin B to treat experimental allergic conjunctivitis in a mouse model.

    abstract:PURPOSE:We evaluated the use of immunological biomarkers in transconjunctival immunotherapy by using cholera toxin B for treating experimental allergic conjunctivitis in a mouse model. METHODS:Balb/c mice were sensitized using intraperitoneal injections of ovalbumin (OVA) and were then divided into two groups. The fir...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1007/s10384-011-0047-x

    authors: Oikawa A,Shoji J,Inada N,Sawa M

    更新日期:2011-09-01 00:00:00

  • Lymphatic sinus-like structures in choroid.

    abstract::Lymphatic sinus-like structures were demonstrated in the choroid of the monkey eye by transmission and scanning electron microscopy. These lymphatic sinus-like structures were lined by fibroblast-like cells with large intercellular gaps. When the specimens were viewed by scanning electron microscopy, the surface morph...

    journal_title:Japanese journal of ophthalmology

    pub_type: 杂志文章

    doi:

    authors: Sugita A,Inokuchi T

    更新日期:1992-01-01 00:00:00