The effect of photopolymerization on stem cells embedded in hydrogels.

Abstract:

:Photopolymerizable hydrogels, formed by UV-exposure of photosensitive polymers in the presence of photoinitiators, are widely used materials in tissue engineering research employed for cellular entrapment and patterning. During photopolymerization, the entrapped cells are directly exposed to polymer and photoinitiator molecules. To develop strategies that prevent potential photoexposure-damage to osteoprogenitor cells, it is important to further characterize the effects of photopolymerization on the exposed cells. In this study we analyzed the viability, proliferation and osteogenic differentiation of multipotent stromal cell (MSC) monolayers after exposure to UV-light in the presence of Irgacure 2959, a frequently used photoinitiator in tissue engineering research. Cell cycle progression, apoptosis and osteogenic differentiation of encapsulated goat MSCs were studied in photopolymerized methacrylate-derivatized hyaluronic acid hydrogel and methacrylated hyperbranched polyglycerol gel. We demonstrate adverse effects of photopolymerization on viability, proliferation and reentry into the cell cycle of the exposed cells in monolayers, whereas the MSCs retain the ability to differentiate towards the osteogenic lineage. We further show that upon encapsulation in photopolymerizable hydrogels the viability of the embedded cells is unaffected by the photopolymerization conditions, while osteogenic differentiation depends on the type of hydrogel used.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Fedorovich NE,Oudshoorn MH,van Geemen D,Hennink WE,Alblas J,Dhert WJ

doi

10.1016/j.biomaterials.2008.09.037

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

344-53

issue

3

eissn

0142-9612

issn

1878-5905

pii

S0142-9612(08)00705-9

journal_volume

30

pub_type

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