Abstract:
OBJECTIVE:Outcome in osteochondral allografting is limited by the immunological incompatibility of the grafted tissue. Based on a resistance of chondrocytes to photodynamic therapy in cell culture it is proposed that 5-aminolevulinic acid-based photodynamic therapy (5-ALA-PDT) might be used to inactivate bone while maintaining viability of chondrocytes and thus immunomodulate bone selectively. METHODS:Chondrocytes and osteoblasts from porcine humeral heads were either isolated (cell culture) or treated in situ (tissue culture). To quantify cytotoxic effects of 5-ALA-PDT (0-20 J/cm(2), 100 mW/cm(2)) an (3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide) (MTT)-assay was used in cell culture and in situ hybridization in tissue culture to assess metabolic active cells (functional osteoblasts: col alpha(1)(I) mRNA, functional chondrocytes: col alpha(1)(II) mRNA). RESULTS:In cell culture, survival after 5-ALA-PDT was significantly higher for chondrocytes (5 J/cm(2): 87+/-12% compared to untreated cells) than for osteoblasts (5J/cm(2): 12+/-11%). In tissue culture, the percentage of functional chondrocytes in cartilage showed a decrease after 5-ALA-PDT (direct fixation: 92+/-2%, 20 J/cm(2): 35+/-15%; P<0.0001). A significant decrease in the percentage of bone surfaces covered by functional osteoblasts was observed in freshly harvested (31+/-3%) compared to untreated tissues maintained in culture (11+/-4%, P<0.0001), with no further decrease after 5-ALA-PDT. CONCLUSION:Chondrocytes were more resistant to 5-ALA-PDT than osteoblasts in cell culture, while in tissue culture a loss of functional chondrocytes was observed after 5-ALA-PDT. Since osteoblasts - but not chondrocytes - were sensitive to the tissue culture conditions, devitalized bone with functional cartilage might already be achieved by applying specific tissue culture conditions even without 5-ALA-PDT.
journal_name
Osteoarthritis Cartilagejournal_title
Osteoarthritis and cartilageauthors
Bastian JD,Egli RJ,Ganz R,Hofstetter W,Leunig Mdoi
10.1016/j.joca.2008.08.005subject
Has Abstractpub_date
2009-04-01 00:00:00pages
539-46issue
4eissn
1063-4584issn
1522-9653pii
S1063-4584(08)00294-Xjournal_volume
17pub_type
杂志文章abstract:OBJECTIVE:The purpose of this controlled laboratory study was to determine the efficacy of Interleukin-1 Receptor Antagonist Protein (IRAP) treatment as an early intervention strategy by examining the changes in microRNA and mRNA expression in cartilage in an ex-vivo porcine knee joint impact model. METHODS:Custom imp...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2015.05.010
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVE:To describe the reproducibility and validity of six different measurement techniques for knee subchondral bone mineral density (sBMD). METHODS:A consecutive sample of 50 male and female participants from a population-based longitudinal study had sBMD assessed using dual energy X-ray absorptiometry scans. Ant...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2008.04.012
更新日期:2008-12-01 00:00:00
abstract:OBJECTIVE:The prevalence of osteoarthritis (OA) varies between joints. Cartilage in eight different joints was evaluated to elucidate the disparate susceptibilities between joints to post-traumatic OA (PTOA) and provide evidence for joint-specific clinical treatments. The hypothesis was that cartilage in different join...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2015.02.023
更新日期:2015-07-01 00:00:00
abstract:OBJECTIVE:Prospectively monitor how treatment of acutely ruptured anterior cruciate ligament (ACL) affects biomarkers of inflammation and proteolytic degradation over 5 years. DESIGN:We studied 119 subjects with acute ACL injury from the randomized controlled knee anterior cruciate ligament, non-surgical versus surgic...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joca.2017.05.009
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVE:To provide an insightful summary of studies on biochemical markers for osteoarthritis (OA). DESIGN:Two investigators systematically searched the electronic PubMed database for clinical studies into soluble biochemical markers for OA in humans that were published between 01-03-2018 and 01-03-2019. Data from s...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2019.11.007
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVE:Our objective was to investigate relationships between proximal tibial subchondral bone mineral density (BMD) and nocturnal pain in patients with knee osteoarthritis (OA). METHODS:The preoperative knee of 42 patients booked for knee arthroplasty was scanned using quantitative computed tomography (QCT). Pain ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2015.04.012
更新日期:2015-09-01 00:00:00
abstract::In this brief abbreviated review of regulatory issues regarding the development of drugs and or devices for the treatment of osteoarthritis (OA), the steps that are expected by both the Food and Drug Administration (FDA) and the European Medicines Agency (EMA) are discussed. ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2015.03.010
更新日期:2015-05-01 00:00:00
abstract:OBJECTIVE:The effects of mechanical deformation of intact cartilage tissue on chondrocyte biosynthesis in situ have been well documented, but the mechanotransduction pathways that regulate such phenomena have not been elucidated completely. The goal of this study was to examine the effects of tissue deformation on the ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2004.08.004
更新日期:2004-12-01 00:00:00
abstract:OBJECTIVES:The etiology of degenerative meniscus tear is unclear but could be related to a generalized osteoarthritic disease process. We studied whether radiographic hand osteoarthritis (OA) is associated with meniscus damage. METHODS:We examined 974 persons aged 50-90 years drawn via census tract data and random-dig...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2015.08.005
更新日期:2016-02-01 00:00:00
abstract:OBJECTIVES:To systematically appraise the evidence on measurement properties of performance-based outcome measures to assess physical function in young and middle-aged people known to be at high risk of hip and/or knee osteoarthritis (OA). METHODS:Electronic searches were performed in MEDLINE, CINAHL, Scopus and SPORT...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2013.10.021
更新日期:2014-01-01 00:00:00
abstract::To highlight methodological challenges in the design and conduct of randomized trials of surgical interventions and to propose strategies for addressing these challenges. This paper focuses on three broad areas: enrollment; intervention; and assessment including implications for analysis. For each challenge raised in ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2015.02.024
更新日期:2015-05-01 00:00:00
abstract:OBJECTIVES:Using a mouse surgical model of post-traumatic osteoarthritis (OA), we sought to determine if muscle function is altered following acute joint injury and whether this relates to OA progression. DESIGN:Male C57BL/6 mice underwent surgical transection of the medio-meniscal tibial ligament destabilisation of t...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2015.12.008
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVE AND METHODS:To evaluate the immune-modulator effect of chondroitin sulfate (CS) by means of the review of the literature. RESULTS:Inflammatory reactions are primarily originated by infectious agents, immune reactions and by sterile tissue lesions that activate membrane receptors by means of pathogen-associat...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2010.02.017
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVE:This narrative review covers original research publications related to imaging advancements in osteoarthritis (OA) published in the English language between 1st April 2016 and 30th April 2017. METHODS:Relevant human studies (excluding pre-clinical and in vitro studies), were searched and selected from PubMed...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2018.01.007
更新日期:2018-03-01 00:00:00
abstract:OBJECTIVE:We investigated whether innate differences in cytokine response were associated with the absence of osteoarthritis (OA) in old age. DESIGN:In 82 participants from a cross-sectional birth cohort, radiographs of hands, hips and knees were taken at the age of 90 years. OA was defined as a Kellgren-Lawrence scor...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2010.03.016
更新日期:2010-07-01 00:00:00
abstract:OBJECTIVE:The relative differences in effectiveness of subchondral stimulation, osteochondral grafts, and autologous chondrocyte implantation (ACI) are still unclear. It is the objective of this study to systematically review the literature on ACI compared to other treatments by clinical outcome and the quality of the ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2010.03.005
更新日期:2010-06-01 00:00:00
abstract::The design and execution of prevention trials for OA have methodological issues that are distinct from trials designed to impact prevalent disease. Disease definitions and their precise and sensitive measurement, identification of high-risk populations, the nature of the intervention (pharmaceutical, nutraceutical, be...
journal_title:Osteoarthritis and cartilage
pub_type: 共识发展会议,杂志文章
doi:10.1016/j.joca.2010.10.031
更新日期:2011-05-01 00:00:00
abstract:OBJECTIVE:Fetal cartilage anlage provides a framework for endochondral ossification and organization into articular cartilage. We previously reported differences between mechanical properties of talar cartilage anlagen and adult articular cartilage. However, the underlying development-associated changes remain to be es...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2011.07.013
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVE:To test whether radiographically normal knees with contralateral radiographic knee osteoarthritis (OA), but without contralateral trauma history, display greater cartilage thickness loss than knees from subjects with bilaterally radiographically normal knees. METHODS:828 radiographically normal knees (Kellgr...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2018.10.006
更新日期:2019-02-01 00:00:00
abstract::In this communication, we discuss some key issues surrounding the translation of preclinical efficacy studies in models of painful osteoarthritis (OA) to the clinical arena. We highlight potential pitfalls which could negatively impact successful translation. These include lack of alignment between a model + endpoint ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2016.08.007
更新日期:2017-03-01 00:00:00
abstract:OBJECTIVE:The purpose of this paper is to review changes in the biomechanical properties of tendons and ligaments from joint disuse. METHOD:We have reviewed 37 experimental studies on joint disuse, which have been carried out with various models of disuse and with various animals. RESULTS:Immobilization of joints has...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1053/joca.1998.0167
更新日期:1999-01-01 00:00:00
abstract:OBJECTIVE:To explore effects of weight loss and maintenance on serum cartilage biomarkers denaturation neoepitope for Collagen2 (Coll2-1) and Fibulin3 fragment (Fib3-2), as well as correlations between Coll2-1 and Fib3-2 and symptomatic improvement, in a knee osteoarthritis (KOA) population. DESIGN:192 obese KOA patie...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joca.2017.06.009
更新日期:2017-10-01 00:00:00
abstract:OBJECTIVE:To investigate the validity of joint space width (JSW) measurements in millimeters (mm) in hand osteoarthritis (OA) patients by comparison to controls, grading of joint space narrowing (JSN), and clinical features. METHODS:Hand radiographs of 235 hand OA patients (mean age 65 years, 83% women) and 471 contro...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2011.08.011
更新日期:2011-11-01 00:00:00
abstract:OBJECTIVE:To evaluate differences in tibial trabecular bone (TB) texture between subjects with and without radiographic knee osteoarthritis (OA) using a variance orientation transform (VOT) method. DESIGN:Subjects with knee OA (Kellgren & Lawrence grade > or =2) and controls without OA (both n=26, seven women) were ma...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2010.01.002
更新日期:2010-05-01 00:00:00
abstract:OBJECTIVES:MicroRNAs (miRNAs) play an important role in the regulation of chondrogenesis of mesenchymal stem cells, but their expression still remains unknown in human adipose-derived stem cells (hADSCs). In this study the miRNA expression profile during chondrogenic differentiation of hADSC and the potential mechanism...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2012.08.024
更新日期:2012-12-01 00:00:00
abstract::Animal models of osteoarthritis (OA) are essential tools for investigating the development of the disease on a more rapid timeline than human OA. Mice are particularly useful due to the plethora of genetically modified or inbred mouse strains available. The majority of available mouse models of OA use a joint injury o...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2015.05.009
更新日期:2015-10-01 00:00:00
abstract:OBJECTIVE:Lateral wedge insoles are a potential simple treatment for medial knee osteoarthritis (OA) patients by reducing the external knee adduction moment (EKAM). However in some patients, an increase in their EKAM is seen. Understanding the role of the ankle joint complex in the response to lateral wedge insoles is ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joca.2015.02.164
更新日期:2015-08-01 00:00:00
abstract:OBJECTIVES:Curcumin (diferuloylmethane) is the principal biochemical component of the spice turmeric and has been shown to possess potent anti-catabolic, anti-inflammatory and antioxidant, properties. This article aims to provide a summary of the actions of curcumin on articular chondrocytes from the available literatu...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2009.10.002
更新日期:2010-02-01 00:00:00
abstract:OBJECTIVE:To employ a magnetic resonance (MR) imaging technique for quantitative assessment of thin cartilage layers, and to validate the cartilage volume and thickness measurements. METHODS:We investigated 10 normal elbow joints (age 20 to 69 years) with a 3D gradient echo sequence with selective water excitation (TR...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1053/joca.1999.0278
更新日期:2000-03-01 00:00:00
abstract:OBJECTIVE:Characterize the effects of alendronate (ALN) on the repair process of the osteonecrotic femoral head as well as the development of secondary osteoarthritis in the ipsilateral hip in an established experimental model of osteonecrosis. METHODS:Osteonecrosis of the femoral head was induced surgically in 60 adu...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2008.07.013
更新日期:2009-03-01 00:00:00