Abstract：
:Inhibitor studies and mutant analysis revealed a C(30) pathway via 4,4'-diapophytoene and 4,4'-diaponeurosporene to 4,4'-diaponeursoporene-4-oic acid esters related to staphyloxanthin in Halobacillus halophilus. Six genes may be involved in this biosynthetic pathway and could be found in two adjacent gene clusters. Two genes of this pathway could be functionally assigned by functional pathway complementation as a 4,4'-diapophytoene synthase and a 4,4'-diapophytoene desaturase gene. These genes were organized in two operons together with two putative oxidase genes, a glycosylase and an acyl transferase ortholog. Pigment mutants were obtained by chemical mutagenesis. Carotenoid analysis showed that a white mutant accumulated 4,4'-diapophytoene due to a block in desaturation. In a yellow mutant carotenogenesis was blocked at the stage of 4,4'-diaponeurosporene and in an orange mutant at the stage of 4,4'-diaponeurosporene-4-oic acid. The protective function of these pigments could be demonstrated for H. halophilus after inhibition of carotenoid synthesis by initiation of oxidative stress. A degree of oxidative stress which still allowed 50% growth of carotenogenic cells resulted in the death of the cells devoid of colored carotenoids.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Köcher S,Breitenbach J,Müller V,Sandmann Gdoi
10.1007/s00203-008-0431-1subject
Has Abstractpub_date
2009-02-01 00:00:00pages
95-104issue
2eissn
0302-8933issn
1432-072Xjournal_volume
191pub_type
杂志文章abstract：:In this study, two quorum sensing (QS) system genes, las and rhI; N-3-oxo-dodecanoyl homoserine lactone (AHL; 3-O-C12-HSL); and QS-related virulence factors and correlation between them were assessed in 30 fish origin P. aeruginosa isolates. The detection of two QS system of the isolates, and eight gene regions consis...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02159-5
更新日期：2021-01-04 00:00:00
abstract：:The gut microbiota inhabits the animal intestinal tract, and dysbiosis of the gut microbiota may result in disease. Senecio scandens has pharmaceutical antibacterial activities and is regarded as a broad-spectrum antibiotic in traditional Chinese medicine. Extracts of S. scandens are reported to show strong antimicrob...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02144-y
更新日期：2021-01-04 00:00:00
abstract：:The L-alanine mediated germination of food isolated Bacillus cereus DSA 1 spores, which lacked an intact exosporium, increased in the presence of D-cycloserine (DCS), which is an alanine racemase (Alr) inhibitor, reflecting the activity of the Alr enzyme, capable of converting L-alanine to the germination inhibitor D-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0946-y
更新日期：2014-02-01 00:00:00
abstract：:Triacylglycerol granules synthesized and accumulated by Rhodococcus opacus and Rhodococcus ruber were isolated by glycerol density gradient centrifugation. Whereas only one type of granule could be isolated from R. opacus, two types of granules with different specific densities were isolated from R. ruber. Both types ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-001-0355-5
更新日期：2001-12-01 00:00:00
abstract：:To clarify the deactivation mechanism of pyruvate formate-lyase (PFL) and its role in the regulation of fermentation in Streptococcus bovis, the molecular properties and genetic expression of multifunctional alcohol dehydrogenase (ADHE) were investigated. S. bovis was found to have ADHE, which was deduced to consist o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0638-0
更新日期：2004-02-01 00:00:00
abstract：:Cadmium is a non-essential toxic heavy metal for organisms, including plants and cyanobacteria. Cadmium resistance transporters involved in resistance of cells against various toxicants such as drugs and effluxes cytotoxic compounds from cells. However, cadmium resistance-associated protein (CadD) has never been repor...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1462-2
更新日期：2018-04-01 00:00:00
abstract：:Representative strains of Gardnerella vaginalis were degraded using both an alkaline and an acid methanolysis and the fatty acid methyl esters released examined by thin-layer and gas chromatography. The profiles obtained were both qualitatively and quantitatively similar and were comprised of straight chain saturated ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425410
更新日期：1984-05-01 00:00:00
abstract：:The diversity among 45 cyanobacterial isolates from 11 different Gunnera species originating from different geographical areas was examined. By means of polymerase chain reaction (PCR) fingerprinting with short tandemly repeated repetitive (STRR) sequences as primers, ten groups of symbiotic cyanobacteria and five uni...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002039900113
更新日期：2000-02-01 00:00:00
abstract：:Synechococcus elongatus strain PCC7942 cells were grown in high or low environmental concentrations of inorganic C (high-C(i), low-C(i)) and subjected to a light shift from 50 micromol m(-2) s(-1) to 500 micromol m(-2) s(-1). We quantified photosynthetic reductant (O(2) evolution) and molar cellular contents of phycob...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0761-1
更新日期：2005-03-01 00:00:00
abstract：:The interaction between Shigella dysenteriae or Shigella sonnei and Acanthamoeba castellanii was studied by viable counts, gentamicin assay and electron microscopy. The result showed that Shigella dysenteriae or Shigella sonnei grew and survived in the presence of amoebae for more than 3 weeks. Gentamicin assay showed...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0422-2
更新日期：2009-01-01 00:00:00
abstract：:An unidentified filamentous purple bacterium, probably belonging to a new genus or even a new family, is found in close association with the filamentous, mat-forming cyanobacterium Microcoleus chthonoplastes in a hypersaline pond at Guerrero Negro, Baja California Sur, Mexico, and in Solar Lake, Sinai, Egypt. This or...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00463477
更新日期：1987-01-01 00:00:00
abstract：:The presence of one periplasmic [NiFe] hydrogenase, one periplasmic [Fe] hydrogenase, and one cytoplasmic NADP-reducing hydrogenase has been previously established in Desulfovibrio fructosovorans. In the present work, marker-exchange mutagenesis was performed to determine the function of the tetrameric NADP-reducing h...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050414
更新日期：1997-01-01 00:00:00
abstract：:The role of the genomic bipyrimidine nucleotide frequency in pyrimidine dimer formation caused by germicidal UV radiation was studied in three microbial reference organisms (Escherichia coli K12, Deinococcus radiodurans R1, spores and cells of Bacillus subtilis 168). The sensitive HPLC tandem mass spectrometry assay w...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0579-3
更新日期：2010-07-01 00:00:00
abstract：:A novel coccoid-shaped, hyperthermophilic, heterotrophic member of the archaea was isolated from a shallow marine hydrothermal system at Vulcano Island, Italy. The isolate grew between 56 and 90 degrees C with an optimum around 85 degrees C. The pH range for growth was 6.5 to 10.5, with an optimum around 9.0. Polysulf...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：:Cellulomonas uda (DSM 20108/ATCC 21399) is one of the few described cellulolytic facultative anaerobes. Based on these characteristics, we initiated a physiological study of C. uda with the aim to exploit it for cellulase production in simple bioreactors with no or sporadic aeration. Growth, cellulase activity and fer...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1230-8
更新日期：2016-10-01 00:00:00
abstract：:Tetrahydrofolate was shown to function as a methyl acceptor in the anaerobic demethylation of dimethylsulfoniopropionate to methylthiopropionate in cell extracts of the sulfate-reducing bacterium strain WN. Dimethylsulfoniopropionate-dependent activities were 0.56 micromol methyltetrahydrofolate min-1 (mg protein)-1 a...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050545
更新日期：1998-01-01 00:00:00
abstract：:We previously constructed an arabinose-inducible recombinant Bifidobacterium longum that could efficiently express secreted IFN-alpha2b in vitro (Deng et al. in Arch Microbiol 191:681-686, 2009). Here, we investigated the influence of oral pBAD-SPIFN-transformed B. longum on immunomodulation and IFN-induced Mx1 gene t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0589-1
更新日期：2010-08-01 00:00:00
abstract：:Chlorobenzoates (CBA) arise as intermediates during the degradation of polychlorinated biphenyls (PCBs) and some chlorinated herbicides. Since PCBs were produced as complex mixtures, a range of mono-, di-, and possibly trichloro-substituted benzoates would be formed. Chlorobenzoate degradation has been proposed to be ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF01692864
更新日期：1996-03-01 00:00:00
abstract：:The molecular weight of the protein moiety of a phospholipoprotein complex isolated from Pseudomonas facilis has been examined with a variety of sodium dodecylsulfate-polyacrylamide gel electrophoretic systems. A molecular weight of 35 000 was determined for the protein in all analyses. A 35 000-dalton protein was pre...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00427842
更新日期：1977-10-24 00:00:00
abstract：:The natural role of antibiotics in the ecology of Streptomyces is debated and still largely unknown. The predatory myxobacteria and many other genera of prokaryotic epibiotic and wolfpack predators across different taxa possess secondary metabolites with antimicrobial action, and these compounds have a role in predati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-0961-7
更新日期：2014-04-01 00:00:00
abstract：:Nitrogen (N) is among the most important macro-nutrients significantly affecting plant growth and yield production. Accordingly, N must be supplied adequately so that optimum amounts of yield are resulted. There are different ways of supplying N to the plant including the use of chemical and biological fertilization. ...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-010-0657-6
更新日期：2011-02-01 00:00:00
abstract：:The alpha-glucosidase (alpha-D-glucoside glucohydrolase, EC 3.2.1.20) of Pseudomonas fluorescens W was partially purified by (NH4)2SO4 fractionation, Sephadex G-200 and DEAE-cellulose column chromatography. The enzyme showed great specificity for maltose hydrolysis, with very little action against polymeric forms. Suc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425338
更新日期：1976-04-01 00:00:00
abstract：:We investigated the structural and immunochemical characteristics of cell wall mannan obtained from Candida sojae JCM 1644, which is a new yeast species isolated from defatted soybean flakes. The results of a slide-agglutination test and of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0339-1
更新日期：2008-05-01 00:00:00
abstract：:We report on the genome-wide response, based on DNA microarrays, of the cyanobacterium Synechocystis sp. PCC 6803 wild type and DeltasigB to a 15 min heat shock. Approximately 9% of the genes in wild type and DeltasigB were significantly regulated (P < 0.001) following this treatment, with chaperones induced the most....
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0138-0
更新日期：2006-10-01 00:00:00
abstract：:A Gram-staining-negative, non-spore-forming, non-flagellated, rod-shaped, catalase- and oxidase-negative bacterium, designated as Gsoil 193(T), was isolated from the soil of ginseng field in Pocheon province, South Korea. 16S rRNA gene sequence analysis showed that strain Gsoil 193(T) belonged to family Xanthomonadace...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1214-8
更新日期：2016-08-01 00:00:00
abstract：:Recently, it was proposed that some antibiotics stimulate the production of reactive oxygen species (ROS), which contribute to cell death. Later, other research groups have provided arguments against ROS-mediated killing of bacteria by antibiotics. At present, there remain a number of unanswered questions in understan...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1247-z
更新日期：2016-11-01 00:00:00
abstract：:Coagulase-negative staphylococci (CNS) are opportunistic pathogens that are currently emerging as causative agents of human disease. Though CNS are widespread in the clinic and food, their precise identification at species level is important. Here, using 16S rRNA sequencing, 55 staphylococcal isolates were identified ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1415-9
更新日期：2018-01-01 00:00:00
abstract：:BvgAS is a two-component system of Bordetella pertussis involved in the reciprocal regulation of the virulence genes and the flagellar biosynthesis. In this study, we found that expression of bvgAS in Escherichia coli also results in reduced motility. The repression was relieved by the addition of known chemical modul...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050688
更新日期：1999-01-01 00:00:00
abstract：:Both the changes in the activities of nitrogenase, glutamine synthetase and glutamate dehydrogenase and in the extracellular and intracellular NH4+ concentrations were investigated during the transition from an NH4+ free medium to one containing NH4+ ions for a continuous culture of Azotobacter vinelandii. If added in...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447319
更新日期：1975-06-22 00:00:00
abstract：:The synthesis of RNA was studied during the synchronous germination of Blastocladia ramosa zoospores. Comparison of RNA synthesis during germination of B. ramosa and Blastocladiella emersonii zoospores revealed that B. ramosa has a longer lag time before RNA synthesis is initiated and, in addition, the rate of RNA syn...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446529
更新日期：1975-12-31 00:00:00
