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Comparison of predicted and experimental subcellular localization of two putative rat steroid dehydrogenases from the short-chain dehydrogenase/reductase protein superfamily.

Abstract：

:In the characterization of newly identified proteins, subcellular localization studies can provide important hints to the proteins' metabolic functions. Depending on the biochemical task of an enzyme, certain subcellular environmental conditions as pH or availability of cofactors and substrates have to be fulfilled. Consequently, misdirected proteins often cannot conduct the proper chemical reaction. This study is aimed at detecting differences in bioinformatic and wet lab experiments and presenting ways for reliable analysis of subcellular localization. On a set of ten enzymes from the short-chain dehydrogenase/reductase (SDR) superfamily, we have performed predictions and experimental analyses of subcellular localization. Exemplarily, we show the localization studies on rat short-chain dehydrogenases/reductases dhrs7b and dhrs8. We demonstrate in particular that all of the prediction algorithms tested failed to assign the SDR enzymes to an experimentally verified subcellular compartment.

journal_name

Mol Cell Endocrinol

journal_title

Molecular and cellular endocrinology

authors

Keller B,Meier M,Adamski J

doi

10.1016/j.mce.2008.07.022

subject

Has Abstract

pub_date

2009-03-25 00:00:00

pages

43-6

issue

1-2

eissn

0303-7207

issn

1872-8057

pii

S0303-7207(08)00330-4

journal_volume

301

pub_type

杂志文章

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