Epithelial sodium channel inhibition in primary human bronchial epithelia by transfected siRNA.

abstract：:Mechanical stimulation of the airway epithelium, as would occur during bronchoconstriction, is a potent stimulus and can activate profibrotic pathways. We used DNA microarray technology to examine gene expression in compressed normal human bronchial epithelial cells (NHBE). Compressive stress applied continuously over...

journal_title：American journal of respiratory cell and molecular biology

authors： Chu EK,Cheng J,Foley JS,Mecham BH,Owen CA,Haley KJ,Mariani TJ,Kohane IS,Tschumperlin DJ,Drazen JM

更新日期：2006-12-01 00:00:00

abstract：:Remodeling of the airways, as occurs in asthmatic patients, is associated with the continual presence of inflammatory mediators and Th2 cytokines, especially interleukin (IL)-13, during cycles of epithelial injury and repair. In this study, we examined the effect of IL-13 on well-differentiated normal human bronchial ...

journal_title：American journal of respiratory cell and molecular biology

authors： Booth BW,Adler KB,Bonner JC,Tournier F,Martin LD

更新日期：2001-12-01 00:00:00

abstract：:Mycobacterium avium (MAV) and M. abscessus (MAB) are ubiquitous environmental organisms increasingly recognized to cause chronic lung disease in patients with apparently normal immune function. Little is yet known about their human pathophysiology. Our objective was to examine cytokine and chemokine responses (protein...

journal_title：American journal of respiratory cell and molecular biology

authors： Sampaio EP,Elloumi HZ,Zelazny A,Ding L,Paulson ML,Sher A,Bafica AL,Shea YR,Holland SM

更新日期：2008-10-01 00:00:00

abstract：:To identify mechanisms that allow p185HER2 expression in lung cancer, we performed Western, Southern, and Northern blot analyses of 14 cell lines derived from human non-small cell lung carcinomas and one cell line derived from a human mesothelioma. Human bronchiole epithelial cells and rat type II pneumocytes were fou...

journal_title：American journal of respiratory cell and molecular biology

authors： Kern JA,Robinson RA,Gazdar A,Torney L,Weiner DB

更新日期：1992-04-01 00:00:00

abstract：:Nontypable Haemophilus influenzae (NTHI) is being increasingly recognized as a cause of both adult pneumonia and acute infectious exacerbations in chronic bronchitis. We used a mouse model to study the immune enhancement of pulmonary clearance of NTHI after a primary immunization. BALB/c mice were immunized with whole...

journal_title：American journal of respiratory cell and molecular biology

authors： McGehee JL,Radolf JD,Toews GB,Hansen EJ

更新日期：1989-09-01 00:00:00

abstract：:Airway remodeling is strongly correlated with the progression of chronic obstructive pulmonary disease (COPD). In this study, our goal was to characterize progressive structural changes in site-specific airways, along with the temporal and spatial expression of transforming growth factor (TGF)-β in the lungs of male s...

journal_title：American journal of respiratory cell and molecular biology

authors： Hoang LL,Nguyen YP,Aspeé R,Bolton SJ,Shen YH,Wang L,Kenyon NJ,Smiley-Jewell S,Pinkerton KE

更新日期：2016-06-01 00:00:00

abstract：:Previous work demonstrated that pre-exposure to ozone primes innate immunity and increases Toll-like receptor-4 (TLR4)-mediated responses to subsequent stimulation with LPS. To explore the pulmonary innate immune response to ozone exposure further, we investigated the effects of ozone in combination with Pam3CYS, a sy...

journal_title：American journal of respiratory cell and molecular biology

authors： Oakes JL,O'Connor BP,Warg LA,Burton R,Hock A,Loader J,Laflamme D,Jing J,Hui L,Schwartz DA,Yang IV

更新日期：2013-01-01 00:00:00

abstract：:Metformin has been used as first-line treatment in patients with type 2 diabetes, and is reported to reduce cancer risk and progression by activating the liver kinase B1 (LKB1)-AMP-activated protein kinase (AMPK) pathway. Cisplatin remains the main drug for treating advanced non-small-cell lung cancer. However, drug r...

journal_title：American journal of respiratory cell and molecular biology

authors： Lin CC,Yeh HH,Huang WL,Yan JJ,Lai WW,Su WP,Chen HH,Su WC

更新日期：2013-08-01 00:00:00

abstract：:To investigate mechanisms regulating intra-alveolar coagulation, we studied monolayers of the A549 human lung epithelial cell line. The surface of A549 cells delayed the onset of prothrombin-to-thrombin conversion and prevented total prothrombin consumption in normal plasma compared to plastic cell-free wells. Similar...

journal_title：American journal of respiratory cell and molecular biology

authors： Berry L,Andrew M,Post M,Ofosu F,O'Brodovich H

更新日期：1991-04-01 00:00:00

abstract：:IL-15 has recently been shown to induce the differentiation of functional dendritic cells (DCs) from human peripheral blood monocytes. Since DCs lay in close proximity to epithelial cells in the airway mucosa, we investigated whether airway epithelial cells release IL-15 in response to inflammatory stimuli and thereby...

journal_title：American journal of respiratory cell and molecular biology

authors： Regamey N,Obregon C,Ferrari-Lacraz S,van Leer C,Chanson M,Nicod LP,Geiser T

更新日期：2007-07-01 00:00:00

abstract：:The nose is an attractive source of airway epithelial cells, particularly in populations in which bronchoscopy may not be possible. However, substituting nasal cells for bronchial epithelial cells in the study of airway inflammation depends upon comparability of responses, and evidence for this is lacking. Our objecti...

journal_title：American journal of respiratory cell and molecular biology

authors： McDougall CM,Blaylock MG,Douglas JG,Brooker RJ,Helms PJ,Walsh GM

更新日期：2008-11-01 00:00:00

abstract：:To assess the temporal and spatial expression of the matrix-associated proteoglycan, biglycan, in a model of chronic hyperoxia-induced lung injury, changes in mRNA and protein were examined using Northern blot analyses and immunohistochemistry. Newborn rats were exposed to 85% or 100% oxygen for 6 and 4 wk, respective...

journal_title：American journal of respiratory cell and molecular biology

authors： Veness-Meehan KA,Rhodes DN,Stiles AD

更新日期：1994-11-01 00:00:00

abstract：:Pseudomonas aeruginosa colonizes the lower respiratory tracts of patients with severe bronchiectasis, including cystic fibrosis, a condition associated with increased airway mucus output. We have shown that an extract containing chloroform-soluble extracellular products of P. aeruginosa releases glycoconjugates into t...

journal_title：American journal of respiratory cell and molecular biology

authors： Somerville M,Taylor GW,Watson D,Rendell NB,Rutman A,Todd H,Davies JR,Wilson R,Cole P,Richardson PS

更新日期：1992-01-01 00:00:00

abstract：:Although nitric oxide (NO) is known to inhibit vascular smooth muscle cell proliferation, the subcellular molecular mechanisms involved with the inhibitory signal transduction pathways are uncertain. We investigated the effect of exogenous NO on cell proliferation and the expression of p53, p21, and phosphorylated p42...

journal_title：American journal of respiratory cell and molecular biology

authors： Mizuno S,Kadowaki M,Demura Y,Ameshima S,Miyamori I,Ishizaki T

更新日期：2004-08-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is a potent peptide growth factor specific for vascular endothelial cells, which promotes neovascularization and increases vascular permeability in vivo. Enhanced microvascular permeability and edema are common characteristics of inflammatory and neoplastic disorders. Two proi...

journal_title：American journal of respiratory cell and molecular biology

authors： Nauck M,Roth M,Tamm M,Eickelberg O,Wieland H,Stulz P,Perruchoud AP

更新日期：1997-04-01 00:00:00

abstract：:The glucocorticoids (GC) and retinoids (RA) modulate branching morphogenesis and cytodifferentiation in the developing lung. We investigated downstream target genes that link glucocorticoid stimulation to the achievement of a mature lung in glucocorticoid receptor (GR) knockout mice. All GR(null) mice and approximatel...

journal_title：American journal of respiratory cell and molecular biology

authors： Kaplan F,Comber J,Sladek R,Hudson TJ,Muglia LJ,Macrae T,Gagnon S,Asada M,Brewer JA,Sweezey NB

更新日期：2003-01-01 00:00:00

abstract：:Bronchiolitis obliterans (BO) is an increasingly important lung disease characterized by fibroproliferative airway lesions and decrements in lung function. Occupational exposure to the artificial food flavoring ingredient diacetyl, commonly used to impart a buttery flavor to microwave popcorn, has been associated with...

journal_title：American journal of respiratory cell and molecular biology

authors： Brass DM,Gwinn WM,Valente AM,Kelly FL,Brinkley CD,Nagler AE,Moseley MA,Morgan DL,Palmer SM,Foster MW

更新日期：2017-06-01 00:00:00

abstract：:The purpose of this study was to characterize basolateral anion channels in Calu-3 and normal human bronchial epithelial cells, and their role in anion secretion. Patch clamp studies identified an outwardly rectifying Cl- channel (ORCC), which could be activated by the adenosine receptor agonist 5'-(N-ethylcarboxamido...

journal_title：American journal of respiratory cell and molecular biology

authors： Szkotak AJ,Man SF,Duszyk M

更新日期：2003-12-01 00:00:00

abstract：:Previously it was reported that hyperoxia induced death of the human lung adenocarcinoma cell line (A549 cells) by necrosis, not by apoptosis. This study examined proliferation and death of untransformed human small airway epithelial (SAE) cells in normoxia or hyperoxia in comparison with A549 cells. We tested the hyp...

journal_title：American journal of respiratory cell and molecular biology

authors： Jyonouchi H,Sun S,Abiru T,Chareancholvanich S,Ingbar DH

更新日期：1998-09-01 00:00:00

abstract：:Histamine was one of the first inflammatory mediators thought to be important in the pathophysiology of asthma, but it is not now thought to be a mediator with primary importance in airway constriction. However, histamine has several effects that may be relevant. One of these effects, its immunoregulatory role, has be...

journal_title：American journal of respiratory cell and molecular biology

authors： Bissonnette EY

更新日期：1996-06-01 00:00:00

abstract：:Pulmonary infection with an exaggerated inflammatory response is the major cause of morbidity and mortality in cystic fibrosis (CF). The objective of this study was to determine whether differences in the innate immune system underlie the exaggerated immune response in CF. We established a model that recapitulates the...

journal_title：American journal of respiratory cell and molecular biology

authors： Bruscia EM,Zhang PX,Ferreira E,Caputo C,Emerson JW,Tuck D,Krause DS,Egan ME

更新日期：2009-03-01 00:00:00

abstract：:The differentiated phenotype of the alveolar type II cell is rapidly altered in vitro. To evaluate factors that might influence this process, we isolated and plated rat type II cells in serum-supplemented media to promote adherence and then maintained the cells in a simple nutrient medium in the absence (S- cells) or ...

journal_title：American journal of respiratory cell and molecular biology

authors： Paine R 3rd,Joyce-Brady M,Clement A,Brody JS

更新日期：1990-10-01 00:00:00

abstract：:Unresolved inflammation in the lung is thought to elicit loss of endothelial cell (EC) barrier integrity and impaired lung function. We have shown, in a mouse model of shock/sepsis, that neutrophil interactions with resident pulmonary cells appear central to the pathogenesis of indirect acute lung injury (iALI). Norma...

journal_title：American journal of respiratory cell and molecular biology

authors： Lomas-Neira J,Venet F,Chung CS,Thakkar R,Heffernan D,Ayala A

更新日期：2014-01-01 00:00:00

abstract：:The molecular mechanisms behind the accumulation of hyaluronan during bleomycin-induced lung injury in rats were investigated. The stimulatory effects of bronchoalveolar lavage fluid (BALF) and alveolar macrophage (AM)-conditioned media on hyaluronan synthesis in normal rat lung fibroblast cultures were studied as wel...

journal_title：American journal of respiratory cell and molecular biology

authors： Teder P,Nettelbladt O,Heldin P

更新日期：1995-02-01 00:00:00

abstract：:Asthma arises from the complex interplay of inflammatory pathways in diverse cell types and tissues. We sought to undertake a comprehensive transcriptomic assessment of the epithelium and airway T cells that remain understudied in asthma and investigate interactions between multiple cells and tissues. Epithelial brush...

journal_title：American journal of respiratory cell and molecular biology

authors： Singhania A,Wallington JC,Smith CG,Horowitz D,Staples KJ,Howarth PH,Gadola SD,Djukanović R,Woelk CH,Hinks TSC

更新日期：2018-02-01 00:00:00

abstract：:The persistence of airway hyperresponsiveness (AHR) and serotonergic enhancement of airway smooth muscle (ASM) contraction induced by ozone (O3) plus allergen has not been evaluated. If this mechanism persists after a prolonged recovery, it would indicate that early-life exposure to O3 plus allergen induces functional...

journal_title：American journal of respiratory cell and molecular biology

authors： Moore BD,Hyde DM,Miller LA,Wong EM,Schelegle ES

更新日期：2014-07-01 00:00:00

abstract：:Identification of the genetic loci underlying asthma in humans has been hampered by variability in clinical phenotype, uncontrolled environmental influences, and genetic heterogeneity. To circumvent these complications, the genetic regulation of asthma-associated phenotypes was studied in a murine model. We characteri...

journal_title：American journal of respiratory cell and molecular biology

authors： Ewart SL,Kuperman D,Schadt E,Tankersley C,Grupe A,Shubitowski DM,Peltz G,Wills-Karp M

更新日期：2000-10-01 00:00:00

abstract：:The chemokine TARC is a ligand for the chemokine receptor CCR4 expressed on T helper (Th)2-type CD4 T cells. Allergic airway inflammation is characterized by a local increase in cells secreting Th2-type cytokines. We hypothesized that bronchial epithelial cells may be a source of chemokines known to chemoattract Th2 c...

journal_title：American journal of respiratory cell and molecular biology

authors： Berin MC,Eckmann L,Broide DH,Kagnoff MF

更新日期：2001-04-01 00:00:00

abstract：:We studied the effect of the nitric oxide synthase (NOS) inhibitor asymmetric dimethyl arginine (ADMA) and the inactive enantiomer N G-methyl-D-arginine (D-NMMA) on Pseudomonas aeruginosa infection of the respiratory mucosa in nasal turbinate organ cultures. We also investigated the effect of P. aeruginosa culture fil...

journal_title：American journal of respiratory cell and molecular biology

authors： Dowling RB,Newton R,Robichaud A,Cole PJ,Barnes PJ,Wilson R

更新日期：1998-12-01 00:00:00

abstract：:Type 2 alveolar epithelial cells (AEC2) are regarded as the progenitor population of the alveolus responsible for injury repair and homeostatic maintenance. Depletion of this population is hypothesized to underlie various lung pathologies. Current models of lung injury rely on either uncontrolled, nonspecific destruct...

journal_title：American journal of respiratory cell and molecular biology

authors： Garcia O,Hiatt MJ,Lundin A,Lee J,Reddy R,Navarro S,Kikuchi A,Driscoll B

更新日期：2016-03-01 00:00:00