Abstract:
:The present study describes the enzymatic properties and molecular identification of 5'-nucleotidase in soluble and microsomal fractions from rat cardiac ventricles. Using AMP as a substrate, the results showed that the cation and the concentration required for maximal activity in the two fractions was magnesium at a final concentration of 1 mM. The pH optimum for both fractions was 9.5. The apparent K(m) (Michaelis constant) values calculated from the Eadie-Hofstee plot were 59.7+/-10.4 microM and 134.8+/-32.1 microM, with V(max) values of 6.7+/-0.4 and 143.8+/-23.8 nmol P(i)/min/mg of protein (means+/-S.D., n=4) from soluble and microsomal fractions respectively. Western blotting analysis of ecto-5'-nucleotidase revealed a 70 kDa protein in both fractions, with the major proportion present in the microsomal fraction. The presence of these enzymes in the heart probably has a physiological function in adenosine signalling. Furthermore, the presence of ecto-5'-nucleotidase in the microsomal fraction could have a role in the modulation of the excitation-contraction-coupling process through involvement of the Ca(2+) influx into the sarcoplasmic reticulum. The measurement of maximal enzyme activities in the two fractions highlights the potential capacity of the different pathways of purine metabolism in the heart.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Pochmann D,Innocente AM,Cotomacci G,Barreto-Chaves ML,Sarkis JJdoi
10.1042/BSR20070039subject
Has Abstractpub_date
2008-10-01 00:00:00pages
267-73issue
5eissn
0144-8463issn
1573-4935pii
BSR20070039journal_volume
28pub_type
杂志文章abstract::The effect of sodium butyrate (NaBut) on cell growth was studied in normal rat kidney (NRK) fibroblasts, and in NRK cells stably transfected with either the adenoviral gene E1A (wild-type), or mutated E1A (E1Amut; with a deletion in the CR1 domain), or with the transforming Ha-ras (EJ) gene. The growth of all these ce...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01199053
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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abstract::CYP (cytochrome P450) 3A29 in pigs could be an important candidate gene responsible for xenobiotic metabolism, similar to CYP3A4 in humans. Accordingly, the tissue expression of CYP3A29 mRNA in domestic pigs has been determined by a real-time PCR. The enzymatic properties of CYP3A29, CYP3A4 and PLM (pig liver microsom...
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abstract::MicroRNA-326 (miR-326) was reported to be dysregulated and involved in the progression of multiple cancers. However, the clinical significance, biological role and underlying mechanism of miR-326 in the carcinogenesis of breast cancer are still unclear. In the present study, we showed that miR-326 was down-regulated i...
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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abstract::An antibody was raised to the synthetic pentapeptide pGluHisProGlyLys which, in radioimmunoassay (RIA), could detect the pentapeptide at a level of 10 fmole per tube and exhibited less than 0.5 per cent cross reactivity with a series of related peptides. The RIA was used to demonstrate the presence of C-terminally ext...
journal_title:Bioscience reports
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pub_type: 杂志文章,meta分析
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
pub_type: 杂志文章,评审
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