TRAC in high-content gene expression analysis: applications in microbial population studies, process biotechnology and biomedical research.

Abstract:

:More than a decade of intensive use of microarray technology has flooded the scientific community with genome-wide expression data of diverse biological states. As a result, connection of the expression signatures of a relatively small number of genes related to, for example, disease states, patient responses or toxicological responses has become possible. Development of tools that enable cost- and time-efficient analysis of such signatures from large sample numbers is currently of major interest for research, drug screening and diagnostic purposes. A method named transcript analysis with aid of affinity capture (TRAC) is a novel solution hybridization and bead-based assay enabling multiplex mRNA target detection simultaneously from large sample numbers. Functionality of TRAC has been shown in a number of applications, including microbial quantification, gene expression-based monitoring of biotechnical processes, cell-based cancer marker gene screening and siRNA validation, which are reviewed here.

journal_name

Expert Rev Mol Diagn

authors

Rautio JJ,Satokari R,Vehmaan-Kreula P,Serkkola E,Söderlund H

doi

10.1586/14737159.8.4.379

subject

Has Abstract

pub_date

2008-07-01 00:00:00

pages

379-85

issue

4

eissn

1473-7159

issn

1744-8352

journal_volume

8

pub_type

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