Abstract:
:Nonmuscle myosin light-chain kinase (MYLK) mediates increased lung vascular endothelial permeability in lipopolysaccharide-induced lung inflammatory injury, the chief cause of the acute respiratory distress syndrome. In a lung injury model, we demonstrate here that MYLK was also essential for neutrophil transmigration, but that this function was mostly independent of myosin II regulatory light chain, the only known substrate of MYLK. Instead, MYLK in neutrophils was required for the recruitment and activation of the tyrosine kinase Pyk2, which mediated full activation of beta(2) integrins. Our results demonstrate that MYLK-mediated activation of beta(2) integrins through Pyk2 links beta(2) integrin signaling to the actin motile machinery of neutrophils.
journal_name
Nat Immunoljournal_title
Nature immunologyauthors
Xu J,Gao XP,Ramchandran R,Zhao YY,Vogel SM,Malik ABdoi
10.1038/ni.1628subject
Has Abstractpub_date
2008-08-01 00:00:00pages
880-6issue
8eissn
1529-2908issn
1529-2916pii
ni.1628journal_volume
9pub_type
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