Abstract:
:Accumulating evidence shows that tight junctions (TJs) in the granular layer contribute to the epidermal barrier, suggesting that the regulation of TJ assembly in keratinocytes may provide a clue to understanding the role of barrier formation in epidermis. In this study, we investigated the behavior of TJ-related molecules in cultured human keratinocytes during keratinization induced by transfer to high-calcium medium, and the effect of RNA interference of TJ-related molecules on intercellular permeability and morphological features. The expression of TJ-related molecules and transepithelial electrical resistance were increased by transfer to high-calcium medium. In cells under the same conditions, we observed by freeze-fracture electron microscopy that TJ strands developed on the apposing cell membranes. In contrast, the transepithelial electrical resistance was clearly suppressed when the expression of claudin-1 or occludin was blocked by RNA interference. The morphological features of these knock-down cells were the same as those of MOCK cells, except for a marked decrease in the number of TJ strands. Furthermore, claudin-1 suppression inhibited occludin localization at the cell membrane, whereas suppression of occludin did not influence the localization of claudin-1. These results suggest that claudin-1 plays a crucial role in recruiting occludin to TJs, and that occludin is involved in intercellular barrier function.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Yamamoto T,Saeki Y,Kurasawa M,Kuroda S,Arase S,Sasaki Hdoi
10.1007/s00403-008-0868-8subject
Has Abstractpub_date
2008-10-01 00:00:00pages
517-24issue
9eissn
0340-3696issn
1432-069Xjournal_volume
300pub_type
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00371642
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00511060
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journal_title:Archives of dermatological research
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doi:10.1007/s00403-004-0495-y
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journal_title:Archives of dermatological research
pub_type: 杂志文章
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417384
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00403890
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journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
pub_type: 杂志文章
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00373422
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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