Effects of active and inactive phospholipase D2 on signal transduction, adhesion, migration, invasion, and metastasis in EL4 lymphoma cells.

abstract：:Expression of the angiotensin II type 1 receptor (AT1-R) mRNA in vascular smooth muscle cells (VSMC) is down-regulated by a variety of agonists, including growth factors, agonists of Galphaq protein-coupled receptors, and activators of adenylyl cyclase. To determine whether cAMP-dependent protein kinases (PKA) partici...

journal_title：Molecular pharmacology

authors： Wang X,Murphy TJ

更新日期：1998-09-01 00:00:00

abstract：:Accumulated evidence suggests that dopamine and dopamine D1 agonists can activate phospholipase C in both brain and peripheral tissue. The receptor that mediates the hydrolysis of phosphoinositides has not been identified. The cloned dopamine D1A receptor that is generally thought to be linked to adenylyl cyclase, has...

journal_title：Molecular pharmacology

authors： Friedman E,Jin LQ,Cai GP,Hollon TR,Drago J,Sibley DR,Wang HY

更新日期：1997-01-01 00:00:00

abstract：:The insecticide imidacloprid and structurally related neonicotinoids act selectively on insect nicotinic acetylcholine receptors (nAChRs). To investigate the mechanism of neonicotinoid selectivity, we have examined the effects of mutations to basic amino acid residues in loop D of the nAChR acetylcholine (ACh) binding...

journal_title：Molecular pharmacology

authors： Shimomura M,Yokota M,Ihara M,Akamatsu M,Sattelle DB,Matsuda K

更新日期：2006-10-01 00:00:00

abstract：:Polycyclic aromatic hydrocarbons (PAHs) are widely distributed environmental toxicants derived from sources that include cigarette smoke, petroleum distillation, gas- and diesel-engine exhaust, and charcoal-grilled food. The gastrointestinal tract is the principal route of PAH exposures, even when inhaled. The most th...

journal_title：Molecular pharmacology

authors： Shi Z,Dragin N,Gálvez-Peralta M,Jorge-Nebert LF,Miller ML,Wang B,Nebert DW

更新日期：2010-07-01 00:00:00

abstract：:alpha4beta2 nicotinic acetylcholine receptors (nAChRs) are recognized as the principal nicotine binding site in brain. Recombinant alpha4beta2 nAChR demonstrate biphasic concentration-response relationships with low- and high-EC50 components. This study shows that untranslated regions (UTR) can influence expression of...

journal_title：Molecular pharmacology

authors： Briggs CA,Gubbins EJ,Marks MJ,Putman CB,Thimmapaya R,Meyer MD,Surowy CS

更新日期：2006-07-01 00:00:00

abstract：:We have observed that rodent cell lines (mouse, hamster) contain approximately 10 times the levels of dihydrofolate reductase as human cell lines, yet the sensitivity to methotrexate (ED(50)), the folate antagonist that targets this enzyme, is similar. Our previous studies showed that dihydrofolate reductase protein l...

journal_title：Molecular pharmacology

authors： Hsieh YC,Skacel NE,Bansal N,Scotto KW,Banerjee D,Bertino JR,Abali EE

更新日期：2009-10-01 00:00:00

abstract：:The in vivo bioavailability of exogenous fibroblast growth factor 2 (FGF2) was studied after i.v. injection of uniformly 14C-labeled FGF2 into young rats. 14C-FGF2 was rapidly accumulated in almost all solid organs within 5 min. After 30 min, more than 65% of FGF2 was retained in liver, 4.5% in kidneys, 1.2% in spleen...

journal_title：Molecular pharmacology

authors： Colin S,Jeanny JC,Mascarelli F,Vienet R,Al-Mahmood S,Courtois Y,Labarre J

更新日期：1999-01-01 00:00:00

abstract：:Identifying novel mechanisms to enhance glucagon-like peptide-1 (GLP-1) receptor signaling may enable nascent medicinal chemistry strategies with the aim of developing new orally available therapeutic agents for the treatment of type 2 diabetes mellitus. Therefore, we tested the hypothesis that selectively modulating ...

journal_title：Molecular pharmacology

authors： Willard FS,Wootten D,Showalter AD,Savage EE,Ficorilli J,Farb TB,Bokvist K,Alsina-Fernandez J,Furness SG,Christopoulos A,Sexton PM,Sloop KW

更新日期：2012-12-01 00:00:00

abstract：:Prostaglandin E(2) (PGE(2)), originally discovered as a pro-inflammatory mediator, also inhibits several chemoattractant-elicited neutrophil functions, including adhesion, secretion of cytotoxic enzymes, production of superoxide anions, and chemotaxis. In this study, we have examined the effects of PGE(2) and prostagl...

journal_title：Molecular pharmacology

authors： Burelout C,Thibault N,Levasseur S,Simard S,Naccache PH,Bourgoin SG

更新日期：2004-08-01 00:00:00

abstract：:Ca-dependent, K-stimulated 86Rb efflux, a measure of Ca-activated K conductance in rat brain synaptosomes, was blocked by phenothiazines and haloperidol. Micromolar concentrations of the phenothiazines, fluphenazine and trifluoperazine, and haloperidol, a non-phenothiazine antipsychotic and calmodulin antagonist, sele...

journal_title：Molecular pharmacology

authors： Benishin CG,Krueger BK,Blaustein MP

更新日期：1988-02-01 00:00:00

abstract：:A methylcholanthrene-induced rat sarcoma that can be propagated in vitro or in vivo was evaluated for resistance to antifolates and was found to be relatively resistant to methotrexate and 10-ethyl-10-deazaaminopterin but sensitive to trimetrexate. Rat sarcoma cell extracts contained low levels of dihydrofolate reduct...

journal_title：Molecular pharmacology

authors： Li WW,Lin JT,Schweitzer BI,Bertino JR

更新日期：1991-11-01 00:00:00

abstract：:Previous studies have demonstrated that cotreatment with mitogen activated-protein kinase kinase (MEK) 1/2 inhibitors (e.g., PD184352) and the checkpoint abrogator 7-hydroxystaurosporine (UCN-01) dramatically induces apoptosis in a variety of human leukemia and multiple myeloma cell types. The purpose of this study wa...

journal_title：Molecular pharmacology

authors： Dai Y,Dent P,Grant S

更新日期：2003-12-01 00:00:00

abstract：:Recent clinical studies reveal that selective agonists of group II metabotropic glutamate (mGlu) receptors have robust efficacy in treating positive and negative symptoms in patients with schizophrenia. Group II mGlu receptor agonists also modulate the in vivo activity of psychotomimetic drugs and reduce the ability o...

journal_title：Molecular pharmacology

authors： Benneyworth MA,Xiang Z,Smith RL,Garcia EE,Conn PJ,Sanders-Bush E

更新日期：2007-08-01 00:00:00

abstract：:The firmest candidate among the transmembrane portions of the nicotinic acetylcholine receptor (AChR) to be in contact with the lipid bilayer is the fourth segment, M4. To explore the contribution of alphaM4 amino acid residues of mouse AChR to channel gating, we combined site-directed mutagenesis with single-channel ...

journal_title：Molecular pharmacology

authors： Bouzat C,Roccamo AM,Garbus I,Barrantes FJ

更新日期：1998-07-01 00:00:00

abstract：:The relations between the single high affinity binding sites for azapropazone, phenylbutazone, chlorpropamide, sulfathiazole, and iophenoxate and the binding regions of human serum albumin represented by the marker ligands diazepam, phenol red, salicylate, and warfarin were examined by a series of competition experime...

journal_title：Molecular pharmacology

authors： Kragh-Hansen U

更新日期：1988-08-01 00:00:00

abstract：:Pancreatic cancer is one of the most lethal types of tumors with no effective therapy available; is currently the third leading cause of cancer in developed countries; and is predicted to become the second deadliest cancer in the United States by 2030. Due to the marginal benefits of current standard chemotherapy, the...

journal_title：Molecular pharmacology

authors： Carozzo A,Yaneff A,Gómez N,Di Siervi N,Sahores A,Diez F,Attorresi AI,Rodríguez-González Á,Monczor F,Fernández N,Abba M,Shayo C,Davio C

更新日期：2019-07-01 00:00:00

abstract：:In PC12 cells stably expressing alpha(1A)-adrenergic receptors (ARs), norepinephrine (NE) activates several mitogen-activated protein kinase pathways and causes differentiation (). Using retroviral luciferase reporters, we found that NE also activated both signal transducers and activators of transcription (Stat) and ...

journal_title：Molecular pharmacology

authors： Zhong H,Murphy TJ,Minneman KP

更新日期：2000-05-01 00:00:00

abstract：:A human recombinant L-type Ca2+ channel (alpha1C,77) was coexpressed with the rat angiotensin AT1A receptor in Xenopus laevis oocytes. In oocytes expressing only alpha1C,77 channels, application of human angiotensin II (1-10 microM) did not affect the amplitude or kinetics of Ba2+ currents (IBa). In sharp contrast, in...

journal_title：Molecular pharmacology

authors： Oz M,Melia MT,Soldatov NM,Abernethy DR,Morad M

更新日期：1998-12-01 00:00:00

abstract：:Metabolism of the pulmonary toxin, 4-ipomeanol, in microsomal preparations from rabbit liver and lung and in purified cytochrome P-450-dependent monooxygenase systems was investigated. The rate of formation of reactive electrophilic products from 4-ipomeanol was estimated by measuring covalent binding to protein or gl...

journal_title：Molecular pharmacology

authors： Wolf CR,Statham CN,McMenamin MG,Bend JR,Boyd MR,Philpot RM

更新日期：1982-11-01 00:00:00

abstract：:Multidrug resistance-associated protein 2 (MRP2) transports glutathione conjugates, glucuronide conjugates, and sulfated conjugates of bile acids. In the present study, we examined the role of charged amino acids in the transmembrane domains of rat Mrp2, conserved among MRP families, using the isolated membrane vesicl...

journal_title：Molecular pharmacology

authors： Ito K,Suzuki H,Sugiyama Y

更新日期：2001-05-01 00:00:00

abstract：:The precise mechanism by which the broad-spectrum anti-RNA virus agent ribavirin elicits its in vitro antiviral effect has remained a matter of debate. We have demonstrated that inhibition of cellular inosine monophosphate dehydrogenase (IMPDH) activity, and thus depletion of intracellular GTP pools, is the predominan...

journal_title：Molecular pharmacology

authors： Leyssen P,De Clercq E,Neyts J

更新日期：2006-04-01 00:00:00

abstract：:Agonist stimulation of group III metabotropic glutamate receptors (mGluRs) induces an inhibition of neurotransmitter release from neurons. The group III mGluRs are pharmacologically defined by activation with the glutamate analog L-amino-4-phosphonobutyric acid (L-AP4). The affinities of these receptors for L-AP4 and ...

journal_title：Molecular pharmacology

authors： Rosemond E,Wang M,Yao Y,Storjohann L,Stormann T,Johnson EC,Hampson DR

更新日期：2004-10-01 00:00:00

abstract：:(6R)-5,6,7,8-Tetrahydrobiopterin (BH4), which is synthesized intracellularly from GTP, caused a concentration-dependent increase in rat pheochromocytoma (PC12) cell proliferation when added exogenously. Incubation with sepiapterin, which is converted enzymatically to BH4 within cells, also increased PC12 cell prolifer...

journal_title：Molecular pharmacology

authors： Anastasiadis PZ,States JC,Imerman BA,Louie MC,Kuhn DM,Levine RA

更新日期：1996-01-01 00:00:00

abstract：:Bryostatin 1 and phorbol-12-myristate-13-acetate (PMA) are both potent activators of protein kinase C (PKC), although in primary mouse keratinocytes bryostatin 1 does not induce differentiation and blocks PMA-induced differentiation. We report here that in primary mouse keratinocytes PMA caused translocation of PKC-ep...

journal_title：Molecular pharmacology

authors： Szallasi Z,Denning MF,Smith CB,Dlugosz AA,Yuspa SH,Pettit GR,Blumberg PM

更新日期：1994-11-01 00:00:00

abstract：:Monoclonal antibodies specific for cytochromes P-450 induced by 3-methylcholanthrene (Mab 1-7-1) and phenobarbital (Mab 2-66-3) have been used in an unlabeled peroxidase-antiperoxidase immunohistochemical procedure to investigate the intralobular distribution and induction sites of the hemoproteins within the livers o...

journal_title：Molecular pharmacology

authors： Forkert PG,Mirehouse-Brown P,Park SS,Gelboin HV

更新日期：1988-12-01 00:00:00

abstract：:Ligands that selectively inhibit human α3β2 and α6β2 nicotinic acetylcholine receptor (nAChRs) and not the closely related α3β4 and α6β4 subtypes are lacking. Current α-conotoxins (α-Ctxs) that discriminate among these nAChR subtypes in rat fail to discriminate among the human receptor homologs. In this study, we desc...

journal_title：Molecular pharmacology

authors： Hone AJ,McIntosh JM,Azam L,Lindstrom J,Lucero L,Whiteaker P,Passas J,Blázquez J,Albillos A

更新日期：2015-11-01 00:00:00

abstract：:Hypertriglyceridemia is a frequent complication accompanying the treatment of patients with either retinoids or rexinoids, [retinoid X receptor (RXR)-selective retinoids]. To investigate the cellular and molecular basis for this observation, we have studied the effects of rexinoids on triglyceride metabolism in both n...

journal_title：Molecular pharmacology

authors： Davies PJ,Berry SA,Shipley GL,Eckel RH,Hennuyer N,Crombie DL,Ogilvie KM,Peinado-Onsurbe J,Fievet C,Leibowitz MD,Heyman RA,Auwerx J

更新日期：2001-02-01 00:00:00

abstract：:Batrachotoxin (BTX), from South American frogs of the genus Phyllobates, irreversibly activates voltage-gated sodium channels. Previous work demonstrated that a phenylalanine residue approximately halfway through pore-lining transmembrane segment IVS6 is a critical determinant of channel sensitivity to BTX. In this st...

journal_title：Molecular pharmacology

authors： Li HL,Hadid D,Ragsdale DS

更新日期：2002-04-01 00:00:00

abstract：:We describe here an approach to characterize various lesions induced in DNA by drug treatments, using three parameters: (a) release of single-stranded DNA fragments by cell lysis in dilute alkali, which result from enzymatic strand scission during DNA repair or chemical alterations of DNA; (b) the presence of high mol...

journal_title：Molecular pharmacology

authors： Lönn U,Lönn S

更新日期：1987-07-01 00:00:00

abstract：:An investigation of the conformational profiles of two cyclic delta-selective opioid peptides, [D-Pen2,D-Pen5]-enkephalin and [D-Pen2,L-Pen5]-enkephalin, has been made. The methods and procedures used are more extensive and systematic than those previously reported, involving a combination of nested grid rotations, cy...

journal_title：Molecular pharmacology

authors： Chew C,Villar HO,Loew GH

更新日期：1991-04-01 00:00:00